Research

The research conducted in the laboratories of the CBTBR is internationally recognized. The two nodes of the CBTBR are positioned adjacently on the research continuum. The University of the Witwatersrand (Wits) node of the CBTBR is at the forefront of fundamental research in mycobacterial metabolism that is aimed at identifying, validating and characterizing novel drug targets and vaccine candidates for TB. The major areas of interest in this laboratory include the molecular mechanisms underlying the regulation of dormancy and persistence in M. tuberculosis and the mechanisms of DNA metabolism underlying the development of strain diversification and the evolution of drug resistance. These areas are being investigated using a highly inter-disciplinary approach involving molecular genetics, biochemistry, microbial physiology, bioinformatics, comparative genomics and transcriptomics.

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The Stellenbosch University (SU) node currently occupies a position bridging the gap between basic research and its application in clinical TB research and management. This laboratory is engaged in fundamental research towards the identification and characterisation of novel drug targets and towards a better understanding of the biology of the bacterium, which enables it to avoid destruction in the host and spread rapidly within human populations. It is also at the cutting edge of research to identify novel bacterial and host markers that will considerably shorten the time taken to evaluate new drug and vaccines and to develop new diagnostic tools and new, multidisciplinary approaches for understanding the epidemiology of the disease. Each one of these efforts is aimed at bridging the gap between fundamental research and its clinical application and some have broader application beyond the field of human TB, most notably in areas such as wildlife management and veterinary disease. For these reasons, the SU node has developed an extensive, collaborative clinical research network, which will be essential for the successful application of post-genomic research findings in a clinical setting.

The research focus of the CBTBR thus falls directly within the theme of 'cutting edge technology development for diseases of poverty' identified in the National R&D Strategy of the Department of Science and Technology. The highly integrated, inter- and multidisciplinary approach that is being adopted provides a particularly powerful platform for the training of young scientists on the new-to-established researcher track in state-of-the-art health research. The vast network of collaborations with which the laboratories are involved will also provide considerable international training opportunities for researchers associated with the CBTBR in leading institutions around the world.

Publications and Conferences

2016

Articles in Peer-Reviewed Journals (Total: 90) - click to open or close information

49/90 (54%) of publications were first- and/or last-authored by a member(s) of the CBTBR

Baartzes, N. Stringer, T. Seldon, R., Warner, D.F., de Kock, C., Smith, P.J., Smith, G.S. 2016. Synthesis, characterization and antimicrobial evaluation of mono- and polynuclear ferrocenyl derived amino and imino complexes, J. Organometallic Chem. doi: 10.1016/j.jorganchem.2016.02.033. IF:2.173

Baartzes, N., Stringer, T., Okombo, J., Seldon, R., Warner, D.F., de Kock, C., Smith, P.J., Smith G.S. (2016). Mono- and polynuclear ferrocenylthiosemicarbazones: synthesis, characterisation, and antimicrobial evaluation. J Organometallic Chem. 819:166-172. doi: 10.1016/j.jorganchem.2016.06.032 IF:2.173

Warner, D.F. 2016. Defining a diagnostic gene signature for tuberculosis. Lancet Respir. Med. http://dx.doi.org/10.1016/S2213-2600(16)00063-1 IF:15.328

Wood, R., Morrow, C., Barry, C.E. III, Bryden, W.A., Call, C.J., Hickey, A.J., Rodes, C.E., Scriba, T.J., Blackburn, J., Issarow, C., Mulder, N., Woodward, J., Moosa, A., Singh, V., Mizrahi, V., and Warner, D.F. 2016. Real-time investigation of tuberculosis transmission: Developing the Respiratory Aerosol Sampling Chamber (RASC). PLoS One doi: 10.1371/journal.pone.0146658  IF:3.234

Leinhardt, C., Lönnroth, K., Menzies, D., Balasegaram, M., Chakaya, J., Cobelens, F., Cohn, J., Denkinger, C.M., Evans, T.G., Källenius, G., Kaplan, G., Kumar, A.M.V., Matthiesen, L., Mgone, C.S., Mizrahi, V., Mukadi, Y., Nguyen, V.N., Nordström, A., Sizemore, C.F., Spigelman, M., Quire, S.B., Swaminathan, S., van Helden, P.D., Zumla, A., Weyer, K., Weil, D., and Raviglione, M. PLoS Med. DOI:10.1371/journal.pmed.1001965 IF:13.585

Djaout, K., Singh, V., Boum, Y., Katawera, V., Becker, H.F., Bush, N.G, Hearnshaw, S.J., Pritchard, J.E., Bourbon, P., Madrid, P.B., Maxwell, A., Mizrahi, V., Myllykallio, H., & Ekins, S. Predictive modeling targets thymidylate synthase ThyX in Mycobacterium tuberculosis. Sci. Rep. 2016; Jun 10;6:27792. doi: 10.1038/srep27792. IF:5.228

Kumar, M., Singh, K., Naran, K., Hamzabegovic, F., Hoft, D.F., Warner, D.F., Ruminski, P., Abate, G., and Chibale, K. 2016. Design, Synthesis and Evaluation of Novel Hybrid Efflux Pump Inhibitors for use against Mycobacterium tuberculosis. ACS Infect. Dis. doi: 10.1021/acsinfecdis.6b00111 IF: not available

Naran, K.*, Moosa, A.*, Barry, C.E. III, Boshoff, H.I.M., Mizrahi, V., and Warner, D.F. 2016. Bioluminescent reporters for rapid mechanism of action assignment in tuberculosis drug discovery. Antimicrob. Agents Chemother. doi:10.1128/AAC.01178-16 [*joint first authors] IF:4.415

Singh, V., Donini, S., Pacitto, A., Sala, C., Hartkoorn, R.C., Dhar, N., Keri, G., Ascher, D.B., Mondésert, G., Vocat, A., Lupien, A., Sommer, R., Vermet, H., Lagrange, S., Buechler, J., Warner, D.F., McKinney, J.D., Pato, J., Cole, S.T., Blundell, T.L., Rizzi, M., and Mizrahi, V. 2016. The inosine monophosphate dehydrogenase, GuaB2, is a vulnerable new bactericidal drug target for tuberculosis. ACS Infect. Dis. doi:10.1021/acsinfecdis.6b00102  IF: not available

Singh, V., and Mizrahi, V. Identification and validation of novel drug targets in Mycobacterium tuberculosis. Drug Discovery Today 2016; doi: 10.1016/j.drudis.2016.09.010. [IF=5.625] IF:5.625

Evans, J.C., Trujillo, C., Wang, Z., Eoh, H., Boshoff, H.I.M., Ehrt, S., Schnappinger, D., Rhee, K., Barry, C.E. III and Mizrahi, V. Validation of CoaBC as a bactericidal target in the coenzyme A pathway of Mycobacterium tuberculosis. ACS Infect. Dis. 2016; doi: 10.1021/acsinfecdis.6b00150. IF: not available

Singh, V., Dhar, N., Pato, J., Kolly, G.S., Korduláková, J., Forbák, M., Evans, J.C., Szekely, R.E., Rybnicker, J.L., Svetlíková, Z., Zemanová, J., Santi, I., Signorino-Gelo, F., Rodrigues L., Vocat, A., Covarrubias, A.S., Rengifo, M.G., Johnsson, K., Mowbray, S., Buechler, J., Delorme, V., Brodin, P., Knott, G.W., Ainsa, J., Warner, D.F., Keri, G., Mikušová, K., McKinney, J.D., Cole, S.T.*, Mizrahi, V.* and Hartkoorn, R.C.* Identification of Aminopyrimidine-Sulfonamides as potent modulators of Wag31-mediated cell elongation in mycobacteria. Mol. Microbiol. 2016; doi:10.1111/mmi.13535 [*Joint senior authors] IF:3.761

Majumdar, G., Mbau, R., Singh, V., Warner, D.F., Dragse, M.S., and Mukherjee, R. Genome-wide transposon mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis. In: In vitro mutagenesis: methods and protocols. Methods in Molecular Biology, Springer Science + Business Media, New York, 2017; vol. 1498, ch. 21, pp. 321-334. doi: 10.1007/978-1-4939-6472-7_21  IF: not available

Chengalroyen, M.D., Beukes, G.M., Gordhan, B.G., Streicher, E.M., Churchyard, G., Hafner, R., Warren, R., Otwombe, K., Martinson, N.A. and Kana B.D.* (2016). Detection and quantification of differentially culturable tubercle bacteria in tuberculosis patients. Am J Respir Crit Care Med. 194. p. 1532-1540 IF: 13.118

Su, H.W., Zhu, J.H., Li, H., Cai, R.J., Ealand, C., Wang, X., Chen, Y.X., Kayani, M.U., Zhu, T.F., Moradigaravand, D., Huang, H., Kana, B.D., Javid, B. (2016) The essential mycobacterial amidotransferase GatCAB is a modulator of specific translational fidelity. Nature Microbiol. 1. p. 16147. IF: not available

Gama, N.H., Elkhadir, A.Y., Gordhan, B.G., Kana, B.D., Darkwa, J. and Meyer, D. (2016) Activity of phosphino palladium(II) and platinum(II) complexes against HIV-1 and Mycobacterium tuberculosis. Biometals. 29. p. 637-50.  IF: 2.134

Karat, A.S., Tlali, M., Fielding, K.L., Charalambous, S., Chihota, V.N., Churchyard, G.J., Hanifa, Y., Johnson, S., McCarthy, K., Martinson, N.A., Omar, T., Kahn, K., Chandramohan, D., Grant, A.D. (2016). Measuring mortality due to HIV-associated tuberculosis among adults in South Africa: Comparing verbal autopsy, minimally-invasive autopsy, and research data. PLoS One. 12:e0174097 IF: 3.057

Bronner Murrison, L., Martinson, N., Moloney, R.M., Msandiwa, R., Mashabela, M., Samet, J.M., Golub, J.E. (2016) Tobacco Smoking and Tuberculosis among Men Living with HIV in Johannesburg, South Africa: A Case-Control Study. PLoS One. 11:e0167133 IF: 3.057

Kayigire, X.A., Friedrich, S.O., Karinja, M.N., van der Merwe, L., Martinson, N.A., Diacon, A.H. (2016) Propidium monoazide and Xpert MTB/RIF to quantify Mycobacterium tuberculosis cells. Tuberculosis (Edinb). 2016 Dec;101:79-84. IF: 2.952

Karat, A.S., Omar, T., von Gottberg, A., Tlali, M., Chihota, V.N., Churchyard, G.J., Fielding, K.L., Johnson, S., Martinson, N.A., McCarthy, K., Wolter, N., Wong, E.B., Charalambous, S., Grant, A.D. (2016) Autopsy Prevalence of Tuberculosis and Other Potentially Treatable Infections among Adults with Advanced HIV Enrolled in Out-Patient Care in South Africa. PLoS One. 11: e0166158. IF: 3.057

Lebina, L., Fuller, N., Osoba, T., Scott, L., Motlhaoleng, K., Rakgokong, M., Abraham, P., Variava, E., Martinson, N.A. (2016) The Use of Xpert MTB/Rif for Active Case Finding among TB Contacts in North West Province, South Africa. Tuberc Res Treat. 2016:4282313. IF: not available

Newton-Foot M, Warren R M, Sampson S L, van Helden P D and Gey van Pittius N C. The plasmid-mediated evolution of the mycobacterial ESX (Type VII) secretion systems. BMC Evol. Biol. (2016) 16:62. IF: 3.406

Gallant J L, Viljoen A J, van Helden P D and Wiid I J F. Glutamate Dehydrogenase Is Required by Mycobacterium bovis BCG for Resistance to Cellular Stress. PLoS ONE (2016) 11:e0147706. IF: 3.057

Macuamule C L S, Wiid I J, van Helden P D, Tanner M and Witthuhn R C. Effect of milk fermentation by kefir grains and selected single strains of lactic acid bacteria on the survival of Mycobacterium bovis BCG. Int. J. Food Microbiol. (2016) 217:170-176. IF:3.445

Miller M, Buss P, de Klerk-Lorist L-M, Hofmeyr J, Hausler G, Lyashchenko K, Lane E P, Botha L, Parsons S and van Helden P. Application of Rapid Serologic Tests for Detection of Mycobacterium bovis Infection in Free-Ranging Warthogs (Phacochoerus africanus)-Implications for Antemortem Disease Screening. J. Wildl. Dis. (2016) 52:180-182. IF:1.189

Seifert M, Georghiou S B, Catanzaro D, Rodrigues C, Crudu V, Victor T C, Garfein R S, Catanzaro A and Rodwell T C. MTBDRplus and MTBDRsl Assays: The Absence of Wild Type Probe Hybridization and Implications for the Detection of Drug-resistant Tuberculosis. J. Clin. Microbiol. (2016). IF:3.631

Tomasicchio M, Theron G, Pietersen E, Streicher E, Stanley-Josephs D, van Helden P, Warren R and Dheda K. The diagnostic accuracy of the MTBDRplus and MTBDRsl assays for drug-resistant TB detection when performed on sputum and culture isolates. Sci Rep (2016) 6:17850. IF:5.228

Miller M, Michel A, van Helden P and Buss P. Tuberculosis in Rhinoceros: An Underrecognized Threat? Transbound Emerg Dis (2016). IF:2.714

Theron G, Venter R, Calligaro G, Smith L, Limberis J, Meldau R, Chanda D, Esmail A, Peter J and Dheda K. Xpert MTB/RIF Results in Patients with Previous Tuberculosis: Can We Distinguish True from False Positive Results? Clin. Infect. Dis. (2016). IF: 8.736

Losi M, Knights A J, Mariani F, Altieri A M, Paone G, Loxton A G, Chegou N N, Kenneth J, Alma M G, Colizzi V, Walzl G, Saltini C, Boyle J and Amicosante M. QuantiFERON-TB performance enhanced by novel Mycobacterium tuberculosis-specific antigens. Eur. Respir. J. (2016) 47:660-664. IF: 8.332

Fang Z, Schubert W-D and Gey van Pittius N C. Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3. Biochemistry and Biophysics Reports (2016) 5:448-452. IF: not available

Phelan J E, Coll F, Bergval I, Anthony R M, Warren R, Sampson S L, Gey van Pittius N C, Glynn J R, Crampin A C, Alves A, Bessa T B, Campino S, Dheda K, Grandjean L, Hasan R, Hasan Z, Miranda A, Moore D, Panaiotov S, Perdigao J, Portugal I, Sheen P, de Oliveira Sousa E, Streicher E M, van Helden P D, Viveiros M, Hibberd M L, Pain A, McNerney R and Clark T G. Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages. BMC Genomics (2016) 17:151. IF: 3.867

Miller M, Kruger M, Kruger M, Olea-Popelka F and Buss P. A Scoring system to improve decision making and outcomes in the adaptation of recently captured white rhinoceroses (Ceratotherium Simum) to captivity. J. Wildl. Dis. (2016) 52: S78-85. IF: 1.189

Farhat M R, Sultana R, Lartchouk O, Bozeman S, Galagan J, Sisk P, Stolte C, Nebenzahl-Guimaraes H, Jacobson K, Sloutsky A, Kaur D, Posey J, Kreiswirth B N, Kurepina N, Rigouts L, Streicher E M, Victor T C, Warren R M, van Soolingen D and Murray M. Genetic Determinants of Drug Resistance in Mycobacterium tuberculosis and Their Diagnostic Value. Am. J. Respir. Crit. Care Med. (2016). IF: 13.118

Seifert M, Georghiou S B, Catanzaro D, Rodrigues C, Crudu V, Victor T C, Garfein R S, Catanzaro A and Rodwell T C. MTBDRplus and MTBDRsl Assays: The Absence of Wild Type Probe Hybridization and Implications for the Detection of Drug-resistant Tuberculosis. J. Clin. Microbiol. (2016). IF: 3.631

Sampson S L. Strength in Diversity: Hidden Genetic Depths of Mycobacterium tuberculosis. Trends Microbiol. (2016) 24:82-84. IF: 9.500

Cliff J M, Cho J-E, Lee J-S, Ronacher K, King E C, van Helden P, Walzl G and Dockrell H M. Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. J. Infect. Dis. (2016) 213:485-495. IF: 6.344

Zak D E, Penn-Nicholson A, Scriba T J, Thompson E, Suliman S, Amon L M, Mahomed H, Erasmus M, Whatney W, Hussey G D, Abrahams D, Kafaar F, Hawkridge T, Verver S, Hughes E J, Ota M, Sutherland J, Howe R, Dockrell H M, Boom W H, Thiel B, Ottenhoff T H M, Mayanja-Kizza H, Crampin A C, Downing K, Hatherill M, Valvo J, Shankar S, Parida S K, Kaufmann S H E, Walzl G, Aderem A, Hanekom W A and ACS and GC6-74 cohort study groups . A blood RNA signature for tuberculosis disease risk: a prospective cohort study. Lancet (2016). IF: 44.002

Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden P, Wiid I and Baker B. The Host Response to a Clinical MDR Mycobacterial Strain Cultured in a Detergent-Free Environment: A Global Transcriptomics Approach. PLoS ONE (2016) 11:e0153079. IF: 3.057

Honeyborne I, McHugh T D, Kuittinen I, Cichonska A, Evangelopoulos D, Ronacher K, van Helden P D, Gillespie S H, Fernandez-Reyes D, Walzl G, Rousu J, Butcher P D and Waddell S J. Profiling persistent tubercule bacilli from patient sputa during therapy predicts early drug efficacy. BMC Med (2016) 14:68.  IF: 8.005

Clarke, C., Van Helden, P., Miller, M. & Parsons, S., 2016, ‘Animal-adapted members of the Mycobacterium tuberculosis complex endemic to the southern African subregion’, Journal of the South African Veterinary Association 87(1), a1322. http://dx.doi.org/10.4102/ jsava.v87i1.1322 IF: 0.441

Melendez J, Sánchez C I, Philipsen R H H M, Maduskar P, Dawson R, Theron G, Dheda K and van Ginneken B. An automated tuberculosis screening strategy combining X-ray-based computer-aided detection and clinical information. Sci Rep (2016) 6:25265. IF: 5.228

Cliff J M, Cho J-E, Lee J-S, Ronacher K, King E C, van Helden P, Walzl G and Dockrell H M. Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. J. Infect. Dis. (2016) 213:485-495. IF: 6.344

Corstjens P L A M, Tjon Kon Fat E M, de Dood C J, van der Ploeg-van Schip J J, Franken K L M C, Chegou N N, Sutherland J S, Howe R, Mihret A, Kassa D, van der Vyver M, Sheehama J, Simukonda F, Mayanja-Kizza H, Ottenhoff T H M, Walzl G, Geluk A and AE-TBC consortium. Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa. Clin. Biochem. (2016) 49:22-31. IF: 2.382

du Plessis W J, Walzl G and Loxton A G. B cells as multi-functional players during Mycobacterium tuberculosis infection and disease. Tuberculosis (Edinb) (2016) 97:118-125. IF: 2.952

Losi M, Knights A J, Mariani F, Altieri A M, Paone G, Loxton A G, Chegou N N, Kenneth J, Alma M G, Colizzi V, Walzl G, Saltini C, Boyle J and Amicosante M. QuantiFERON-TB performance enhanced by novel Mycobacterium tuberculosis-specific antigens. Eur. Respir. J. (2016) 47:660-664. IF: 8.332

Pule C M, Sampson S L, Warren R M, Black P A, van Helden P D, Victor T C and Louw G E. Efflux pump inhibitors: targeting mycobacterial efflux systems to enhance TB therapy. J. Antimicrob. Chemother. (2016) 71:17-26. IF: 4.919

Demers A-M, Venter A, Friedrich S O, Rojas-Ponce G, Mapamba D, Jugheli L, Sasamalo M, Almeida D, Dorasamy A, Jentsch U, Gibson M, Everitt D, Eisenach K D and Diacon A H. Direct Susceptibility Testing of Mycobacterium tuberculosis for Pyrazinamide by Use of the Bactec MGIT 960 System. J. Clin. Microbiol. (2016) 54:1276-1281. IF:3.631

Seifert M, Ajbani K, Georghiou S B, Catanzaro D, Rodrigues C, Crudu V, Victor T C, Garfein R S, Catanzaro A and Rodwell T C. A performance evaluation of MTBDRplus version 2 for the diagnosis of multidrug-resistant tuberculosis. Int. J. Tuberc. Lung Dis. (2016) 20:631-637. IF: 2.148

Chegou N N, Sutherland J S, Malherbe S, Crampin A C, Corstjens P L A M, Geluk A, Mayanja-Kizza H, Loxton A G, van der Spuy G, Stanley K, Kotzé L A, van der Vyver M, Rosenkrands I, Kidd M, van Helden P D, Dockrell H M, Ottenhoff T H M, Kaufmann S H E, Walzl G and AE-TBC consortium. Diagnostic performance of a seven-marker serum protein biosignature for the diagnosis of active TB disease in African primary healthcare clinic attendees with signs and symptoms suggestive of TB. Thorax (2016). IF: 8.121

Mbugi E V, Katale B Z, Streicher E M, Keyyu J D, Kendall S L, Dockrell H M, Michel A L, Rweyemamu M M, Warren R M, Matee M I, van Helden P D, Couvin D and Rastogi N. Mapping of Mycobacterium Tuberculosis Complex Genetic Diversity Profiles in Tanzania and Other African Countries. PLoS ONE (2016) 11: e0154571. IF: 3.057

Parsons S D C, McGill K, Doyle M B, Goosen W J, van Helden P D and Gormley E. Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle. PLoS ONE (2016) 11: e0155440. IF: 3.057

Jabot-Hanin F, Cobat A l, Feinberg J, Grange G, Remus N, Poirier C, Boland-Auge A, Besse C l, Bustamante J, Boisson-Dupuis Sp, Casanova J-L, Schurr E, Alcaïs A, Hoal E G, Delacourt C and Abel L. Major Loci on Chromosomes 8q and 3q Control Interferon γ Production Triggered by Bacillus Calmette-Guerin and 6-kDa Early Secretory Antigen Target, Respectively, in Various Populations. J Infect Dis. (2016) 213:1173-1179. IF: 6.344

Jacobs R, Maasdorp E, Malherbe S, Loxton A G, Stanley K, van der Spuy G, Walzl G and Chegou N N. Diagnostic Potential of Novel Salivary Host Biomarkers as Candidates for the Immunological Diagnosis of Tuberculosis Disease and Monitoring of Tuberculosis Treatment Response. PLoS ONE (2016) 11: e0160546. IF: 3.057

du Plessis W J, Keyser A, Walzl G and Loxton A G. Phenotypic analysis of peripheral B cell populations during Mycobacterium tuberculosis infection and disease. J Inflamm (Lond) (2016) 13:23. IF: 1.975

Johnson G. The α/β Hydrolase Fold Proteins of Mycobacterium tuberculosis, With Reference to their Contribution to Virulence. Curr. Protein Pept. Sci. (2016). IF: 2.441

Garcia B J, Loxton A G, Dolganov G M, Van T T, Davis J L, de Jong B C, Voskuil M I, Leach S M, Schoolnik G K, Walzl G, Strong M and Walter N D. Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients. Tuberculosis (Edinb) (2016) 100:89-94. IF: 2.952

Malherbe S T, Shenai S, Ronacher K, Loxton A G, Dolganov G, Kriel M, Van T, Chen R Y, Warwick J, Via L E, Song T, Lee M, Schoolnik G, Tromp G, Alland D, Barry C E, Winter J, Walzl G, Catalysis TB–Biomarker Consortium , Lucas L, Spuy G v d, Stanley K, Theart L, Smith B, Burger N, Beltran C G G, Maasdorp E, Ellmann A, Choi H, Joh J, Dodd L E, Allwood B, Kogelenberg C, Vorster M and Griffith-Richards S. Persisting positron emission tomography lesion activity and Mycobacterium tuberculosis mRNA after tuberculosis cure. Nat. Med. (2016). IF: 30.357

Devonshire A S, O'Sullivan D M, Honeyborne I, Jones G, Karczmarczyk M, Pavšič J, Gutteridge A, Milavec M, Mendoza P, Schimmel H, Van Heuverswyn F, Gorton R, Cirillo D M, Borroni E, Harris K, Barnard M, Heydenrych A, Ndusilo N, Wallis C L, Pillay K, Barry T, Reddington K, Richter E, Mozioğlu E, Akyürek S, Yalçınkaya B, Akgoz M, Žel J, Foy C A, McHugh T D and Huggett J F. The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis. BMC Infect. Dis. (2016) 16:366. IF: 2.690

Esmail H, Lai R P, Lesosky M, Wilkinson K A, Graham C M, Coussens A K, Oni T, Warwick J M, Said-Hartley Q, Koegelenberg C F, Walzl G, Flynn J L, Young D B, Barry III C E, O'Garra A and Wilkinson R J. Characterization of progressive HIV-associated tuberculosis using 2-deoxy-2-[18F] fluoro-D-glucose positron emission and computed tomography. Nat Med (2016) advance online publication. IF: 30.357

Brüns A C, Tanner M, Williams M C, Botha L, O'Brien A, Fosgate G T, Van Helden P D, Clarke J and Michel A L. Diagnosis and Implications of Mycobacterium bovis Infection in Banded Mongooses (Mungos mungo) in the Kruger National Park, South Africa. J. Wildl. Dis. (2016). IF: 1.189

Geldenhuys, E.-M., Burger, E. H., van Helden, P. D., Mole, C. G. and Kotzé, S. H. (2016), Optimizing the use of cadavers by integrating pathology during anatomy dissection. American Association of Anatomists, 9: 575–582. doi:10.1002/ase.1617 IF: 2.303

Ngwane A, Macingwana L, Viljoen A, Ngcete Z, Madikane EV, Baker B, Chibale K, van Helden PD, Wiid IJ. (2016) In vitro activity of F1082, a novel synthetic furanone based antitubercular compound and its potential for TB drug development. IUBMB Life 68(8): 612-620. IF: 2.653

Leisching G, Pietersen R-D, Wiid IJ, Baker B. (2016) Virulence, Biochemistry, Morphology and Host-Interacting Properties of Detergent-Free Cultured Mycobacteria: An Update. Tuberculosis (Edinb). 100:53-60. IF: 2.952

Leisching G, Pietersen R-D, van Heerden C, van Helden PD, Wiid I, Baker B. (2016) Differential host  response  to  clinical  hypo-virulent  and  hyper-virulent  Beijing  strains indicates a role for induced apoptosis in virulence. Virulence, Oct 20, 1-11. IF: 5.418

Chingwaru W, Glashoff RH, Vidmar J, Kapewangolo P, Sampson SL. Mammalian cell cultures as models forMycobacterium tuberculosis-human immunodeficiency virus (HIV) interaction studies: A review. Asian Pacific Journal of Tropical Medicine. 2016 (9):832-838 IF: 0.841

Whitfield MG, Streicher EM, Dolby T, Simpson JA, Sampson SL, Van Helden PD, Van Rie A, Warren RM. Prevalence of pyrazinamide resistance across the spectrum of drug resistant phenotypes of Mycobacterium tuberculosis. Tuberculosis. 2016 (99):128-130 IF: 3.503

Bowker N, Salie M, Schurz H, van Helden PD, Kinnear CJ, Hoal EG, Möller M. 2016. Polymorphisms in the pattern recognition receptor Mincle gene (CLEC4E) and association with tuberculosis. Lung 194(5), 763-767 DOI 10.1007/s00408-016-9915-y. IF: 2.0

van Rensburg IC, Kleynhans L, Keyser A, Walzl G, Loxton AG. B-cells with a FasL expressing regulatory phenotype are induced following successful anti-tuberculosis treatment. Immun Inflamm Dis. 2016 Dec 27;5(1):57-67. doi: 10.1002/iid3.140. eCollection 2017 Mar. PubMed PMID: 28250925; PubMed Central PMCID: PMC5322165. IF: not available

du Plessis WJ, Kleynhans L, du Plessis N, Stanley K, Malherbe ST, Maasdorp E, Ronacher K, Chegou NN, Walzl G, Loxton AG. The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis. PLoS One. 2016 Apr 6;11(4): e0152710. doi: 10.1371/journal.pone.0152710. eCollection 2016. PubMed PMID: 27050308; PubMed Central PMCID: PMC4822853. IF: 3.057

Uren C, Kim M, Martin A R, Bobo D, Gignoux C R, Helden P D v, Möller M, Hoal E G and Henn B M. Fine-Scale Human Population Structure in Southern Africa Reflects Ecogeographic Boundaries. GENETICS September 1,2016 vol. 204 no. 1 303 314; https://doi.org/ 10.1534/genetics.116.187369 IF: 4.644

Glanzmann B, Möller M, le Roex N, Tromp G, Hoal E G and van Helden P D. The complete genome sequence of the African buffalo (Syncerus caffer). BMC Genomics (2016) 17(1):1001 IF: 3.867

Du Plessis N, Jacobs R, Gutschmidt A, Fang Z, van Helden PD, Lutz MB, Hesseling AC, Walzl G. Phenotypically resembling myeloid derived suppressor cells are increased in children with HIV and exposed/infected with Mycobacterium tuberculosis DOI: 10.1002/eji.201646658, Eur J Immunol. Epub 2016 Dec 16. IF: 4.179

Peter, J. G., Zijenah, L. S., Chanda, D., Clowes, P., Lesosky, M., Gina, P.,Theron, G., Dheda, K. (2016). The mortality impact of point-of-care urine lipoarabinomannan testing to guide tuberculosis treatment initiation in HIV-infected hospitalised patients: a multi-country randomised controlled trial. The Lancetin press(15), 1–11. http://doi.org/10.1016/S0140-6736(15)01092-2 IF: 44.002

Theron G, Peter J, Richardson M, Warren R, Dheda K, Steingart KR. (2016) GenoType® MTBDR#sl assay for resistance to second-line anti-tuberculosis drugs. Cochrane Database of Systematic Reviews 2016, Issue 9. Art. No.: CD010705 DOI:10.1002/14651858. CD010705.pub3. IF: 6.665

MD Chengalroyen, GM Beukes, BG Gordhan, EM Streicher, G Churchyard, R Hafner, R Warren, K Otwombe, N Martinson and BD Kana (2016) Detection and Quantification of Differentially Culturable Tubercle Bacteria in Sputum from Patients with Tuberculosis. Am J Respir Crit Care Med, 194(12):1532–1540 IF: 13.118

Awoniyi DO, Teuchert A, Sutherland JS, Mayanja-Kizza H, Howe R, Mihret A, Loxton AG, Sheehama J, Kassa D, Crampin AC, Dockrell HM, Kidd M, Rosenkrands I, Geluk A, Ottenhoff TH, Corstjens PL, Chegou NN, Walzl G; AE-TBC Consortium. Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease. J Infect. 2016 Sep;73(3):219-30. doi: 10.1016/j.jinf.2016.04.036. Epub 2016 Jun 14. PubMed PMID: 27311746. IF:4.382

Jacobs R, Tshehla E, Malherbe S, Kriel M, Loxton AG, Stanley K, van der Spuy G, Walzl G, Chegou NN. Host biomarkers detected in saliva show promise as markers for the diagnosis of pulmonary tuberculosis disease and monitoring of the response to tuberculosis treatment. Cytokine. 2016 May; 81:50-6. doi: 10.1016/j.cyto.2016.02.004. Epub 2016 Feb 13. PubMed PMID: 26878648. IF: 2.94

Corstjens PL, Tjon Kon Fat EM, de Dood CJ, van der Ploeg-van Schip JJ, Franken KL, Chegou NN, Sutherland JS, Howe R, Mihret A, Kassa D, van der Vyver M, Sheehama J, Simukonda F, Mayanja-Kizza H, Ottenhoff TH, Walzl G, Geluk A; AE-TBC consortium. Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa. Clin Biochem. 2016 Jan;49(1-2):22-31. doi: 10.1016/j.clinbiochem.2015.08.013. Epub 2015 Aug 15. PubMed PMID: 26285074. IF: 2.382

Malherbe ST, Walzl G. Can we prevent Tuberculosis transmission? South African Respiratory Journal. 22(4) 2016; 22(4) (editorial) IF: not available

Jacobs R, Malherbe S, Loxton AG, Stanley K, van der Spuy G, Walzl G, Chegou NN. Identification of novel host biomarkers in plasma as candidates for the immunodiagnosis of tuberculosis disease and monitoring of tuberculosis treatment response. Oncotarget. 2016 Sep 6;7(36):57581-57592. doi: 10.18632/oncotarget.11420. PubMed PMID: 27557501; PubMed Central PMCID: PMC5295374. IF: 5.008

Shenai S, Ronacher K, Malherbe S, Stanley K, Kriel M, Winter J, Peppard T, Barry CE, Wang J, Dodd LE, Via LE, Barry CE 3rd, Walzl G, Alland D. Bacterial Loads Measured by the Xpert MTB/RIF Assay as Markers of Culture Conversion and Bacteriological Cure in Pulmonary TB. PLoS One. 2016 Aug 10;11(8):e0160062. doi: 10.1371/journal.pone.0160062. eCollection 2016. PubMed PMID: 27508390; PubMed Central PMCID: PMC4980126. IF: 3.057

du Plessis WJ, Kleynhans L, du Plessis N, Stanley K, Malherbe ST, Maasdorp E, Ronacher K, Chegou NN, Walzl G, Loxton AG. The Functional Response of B Cells to Antigenic Stimulation: A Preliminary Report of Latent Tuberculosis. PLoS One. 2016 Apr 6;11(4): e0152710. doi: 10.1371/journal.pone.0152710. eCollection 2016. PubMed PMID: 27050308; PubMed Central PMCID: PMC4822853. IF: 3.057

Strijdom H, De Boever P, Walzl G, Essop MF, Nawrot TS, Webster I, Westcott C, Mashele N, Everson F, Malherbe ST, Stanley K, Kessler HH, Stelzl E, Goswami N. Cardiovascular risk and endothelial function in people living with HIV/AIDS: design of the multi-site, longitudinal EndoAfrica study in the Western Cape Province of South Africa. BMC Infect Dis. 2017 Jan 7;17(1):41. doi: 10.1186/s12879-016-2158-y. PubMed PMID: 28061822; PubMed Central PMCID: PMC5219697. IF: 2.69

Dambuza I, Keeton R, Hsu NJ, Allie N, Quesniaux VF, Ryffel B, Jacobs M., Persistent p55TNFR expression impairs T cell response during chronic tuberculosis and promotes reactivation. Sci. Rep. 2016, 6,39499. IF: 5.228

Lienhardt C, Lönnroth K, Menzies D, Balasegaram M, Chakaya J, Cobelens F, Cohn J, Denkinger CM, Evans TG, Källenius G, Kaplan G, Kumar AM, Matthiessen L, Mgone CS, Mizrahi V, Mukadi YD, Nguyen VN, Nordström A, Sizemore CF, Spigelman M, Squire SB, Swaminathan S, van Helden PD, Zumla A, Weyer K, Weil D, Raviglione M. Translational research for tuberculosis elimination: Priorities, challenges, and actions. PLOS Medicine. 2016 Mar 2; 13(3): e1001965. [Review] IF: 14.429

Mouton JM, Helaine S, Holden DW, Sampson SL. Elucidating population-wide mycobacterial replication dynamics at the single-cell level. Microbiology 2016. 162:966-978. IF: 3.026

Mouton JM, van der Merwe L, Goosen A, Revera M, Brink PA, Moolman-Smook JC, Kinnear C. MyBPH acts as modifier of cardiac hypertrophy in hypertrophic cardiomyopathy (HCM) patients. Human Genetics. 2016. 135(5):477-83. IF: 4.633

Warren RM. Implementation of new tools for multidrug-resistant tuberculosis detection and control. Int J Mycobacteriol. 2016 Dec;5 Suppl 1: S67. doi: 10.1016/j.ijmyco.2016.08.014. Epub 2016 Sep 21. IF: 0.271

Chisompola NK, Streicher EM, Warren RM, Sampson SL. Genetic diversity in drug resistant clinical isolates of Mycobacterium tuberculosis circulating within Ndola district; a high HIV prevalence district of Zambia. Antimicrobial Resistance and Infection Control 2016, 6(Suppl 1):1. DOI 10.1186/s13756-016-0153-0 IF: 2.716

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total=108)

Invited / Plenary / Keynote Lectures (11) National and International

Mizrahi, V. Identifying vulnerable steps in the CoA biosynthesis pathway of M. tuberculosis. Plenary lecture delivered at the ASBMB Annual Meeting, EB2016, San Diego, 4 April 2016

Mizrahi, V. Targeting core metabolic pathways in Mycobacterium tuberculosis. Invited talk, Chemistry Department, Ben-Gurion University of the Negev, Be’er Sheva, Israel, 7 June 2016

Mizrahi, V. The IMPDH, GuaB2, is a vulnerable bactericidal drug target for TB. Plenary lecture delivered at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

Mizrahi, V. Global health networks and networking. Plenary lecture, Imperial-UCT Graduate Summer School, Global Health Fellows Programme, University of Cape Town, 15 June 2016.

Kana, B.D. Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidases are required for cell division in mycobacteria. EMBO conference on Bacterial morphogenesis, survival and virulence: Regulation in 4D. 27th November – 1 December 2016. Estuary Island Resort, Kerala, India

Kana, B.D. Differentially Culturable Tubercle Bacteria During TB Treatment. Catalysis Bacterial Load Meeting. Bill and Melinda Gates Foundation. 10th – 11th August 2016. Seattle, USA.

Kana, B.D. The Impact of differentially culturable organisms on transmission. Bill and Melinda Gates Foundation Aerobiology Summit. 22nd – 23rd March 2016. The Maxwell Hotel, Cape Town, South Africa.

Kana, B.D. Biomimicry for Innovation. Wits University Innovator’s Forum. 15th September 2016. The Wits Club, University of the Witwaterstand, Johannesburg, South Africa.

Kana, B.D. Remodelling of the mycobacterial cell surface during tuberculosis disease: Separation anxiety and multiple personality disorder in mycobacteria. South African Society of Biochemistry and Molecular Biology. 10th-14th July 2016. East London International Convention Centre, East London, South Africa.

Kana, B.D. Transitions in Academic Careers. DST-NRF Postdoctoral Forum. 13th-15th March 2016. Lagoon Beach Hotel, Cape Town, South Africa.

Tabb DL. Keynote: Quality Control for Proteomics Keynote address presented at the HUPO-PSI 2016 meeting. University of Ghent, Ghent, Belgium, 18-20 April 2016.

Invited Talks (47) National and International

Reiche, M.A., Martin, Z., Lang, D., Dhar, N., McKinney, J.D., Mizrahi, V., and Warner, D.F. Visualizing the Mycobacterial Mutasome. Cape

Mbau, R.D. Whole-genome mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria. Early Career Scientist Conference, SAMRC Conference Centre, Parow, 19-20 October 2016.

Evans, J. Validation of CoaBC as a bactericidal drug target in the coenzyme A pathway of Mycobacterium tuberculosis. Invited talk presented at the 2016 H3D Symposium, Goudini Spa, Western Cape, 15-18 November 2016

Singh, V. The inosine monophosphate dehydrogenase GuaB2 is a vulnerable new bactericidal drug target in Mycobacterium tuberculosis. Invited talk presented at the 2016 H3D Symposium, Goudini Spa, Western Cape, 15-18 November 2016

Ealand, C., Mashigo, L., Mapela, L., Beukes, G., Machowski, E., Chengalroyen, M. and Kana, B. Resuscitation promoting factors are required for biofilm formation in Mycobacterium smegmatis. Oral presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Shaku. TM, Chengalroyen. MD and Kana. B. Characterization of LytM-domain containing proteins in Mycobacterium smegmatis. Oral presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Papadopoulos. A. and Kana. B   Breaking down walls! How do mycobacteria remodel their cell surface? Oral presentation at the Molecular Biosciences Research Thrust, University of the Witwatersrand. 8 December 2016. Won second prize for best oral presentation.

Peters, J.S., McIvor, A., Gordhan, B., Martinson, N., Waja, Z. and Kana B.D. Dynamics in DCTB decline during early treatment of tuberculosis. Oral presentation at the Clinical Symposium TB Centre meeting Vanderbilt University, TN, USA. 27 April 2016

Peters, J.S., McIvor, A., Gordhan, B., Martinson, N., Waja, Z. and Kana B.D. Strain distribution of infecting M. tuberculosis populations in two socio-economically diverse South African communities. Oral presentation at the Soweto Matlosana Collaborative Centre for HIV/AIDS & TB (SoMCAT) young researchers conference. 18 November 2016

McIvor. A., Gordhan. B., Martinson. N. and Kana. B.D. Enhancing detection of Mycobacterium tuberculosis by the BACTEC MGIT 960 culture system. Oral Presentation at the MRC Soweto Matlosana Collaborating Centre for HIV/AIDS and TB Conference (SoMCHAT). 18 November 2016

Ralefeta D., Machowski E. and Kana B.D. Mycobacterial DD-Carboxypetidases: Filling In The Gaps. Oral Presentation at the Wits 7th Cross Faculty Symposium. 1-2 March 2016. Won third prize for an oral presentation from the Faculty of Health Sciences

Senzani. S., Dhar. N. and Kana. B.D. Identification and Characterization of Mycobacterial Cell wall amidases. Oral presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016. Won first prize for an oral presentation by a student in the Infectious Diseases Track

Maphatsoe. M., Ealand. C., and Kana. B. D. Localization of Mycobacterium smegmatis DD- Cpases and cell wall remodeling enzymes. Oral presentation at the Molecular Bioscience Research Thrust (MBRT).  08 December 2016

Chengalroyen M., Said H., Beylis N., da Silva P., Padanilam X., Radebe D., Ramsamy S., Louw R. and Kana B.D. Genotypic and phenotypic heteroresistance of extensively drug resistant TB isolates. Oral presentation at the Somchat Young Researchers Conference. 18 November 2016

Narrandes. N. and Kana. B.D. Characterization of the mycobacterial electron transport chain- Implications for drug efficacy. Oral presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016. Won first prize for an oral presentation by a student in the Molecular and Comparative Biosciences Track

Loxton AG. Discovery of new TB Biomarker Candidates Talk presented at the TB Vaccine meeting 2016. Eurotel Victoria Hotel, Les Diablerets, Switzerland, 21 January-05 February 2016.

Whitfield MG, Streicher EM, Dolby T, Simpson JA, Sampson SL, van Helden PD, van Rie A, Warren RM. Prevalence of pyrazinamide resistance across the spectrum of drug resistant phenotypes of Mycobacterium Tuberculosis Talk presented at the 37th Annual Congress of the European Society of Mycobacteriology. Sheraton Catania Hotel & Conference Center, Catania, Sicily, Italy, 03-06 July 2016.

Whitfield MG, Streicher EM, Dolby T, Simpson JA, Sampson SL, van Helden PD, van Rie A, Warren RM. Prevalence of pyrazinamide resistance across the spectrum of drug resistant phenotypes of Mycobacterium Tuberculosis Talk presented at the 2nd Acid Fast Cape Town Meeting. Stellenbosch University, Cape Town, South Africa, 08 November 2016.

Tabb DL. Studying Bioinformatics at SUN Talk presented at the Square Kilometer Array Data Science Career Workshop. Sol Plaatjie University, Kimberley, South Africa, 07-08 September 2016.

Tabb DL. Quality Control for Proteomics Talk presented at the National Institute of Biological Sciences, Beijing, China, 20 September 2016.

Tabb DL. Publishing in a consortium Talk presented at the Phoenix Center, Beijing, China, 20 September 2016.

Tabb DL. Identification with Unbounded Databases Talk presented at the Institute for Computing Technology, Beijing, China, 21-22 September 2016.

Tabb DL. Publishing in a Consortium Talk presented at the Shanghai Jiao Tong University, Shanghai, China, 28 September 2016.

Van Helden PD. Drug Susceptibility testing: yesterday, today and tomorrow in the context of heteroresistance Talk presented at the Malaria, Tuberculosis and neglected tropical diseases: Progress in drug discovery and development. Goudini spa, Cape Town, South Africa, 15-18 November 2016.

Grobbelaar M, Sampson SL, Louw GE, Victor TC, van Helden PD, Warren RM and de Vos M . Phosphate ABC transporter system regulates the level of rifampicin resistance in Mycobacterium Tuberculosis Talk presented at the 37th Annual Congress of the European Society of Mycobacteriology. Sheraton Catania, Catania, Sicily, 03-06 July 2016.

Willemse D, Weber B, Warren RM, Adinolfi S, Pastore A, Williams MJ. Regulation of iron-sulphur cluster biogenesis in Mycobacterium Tuberculosis Talk presented at the FMHS 60th Annual Academic Year Day. Stellenbosch University, Cape Town, South Africa, 11 August 2016.

Chegou NN. Diagnostic Performance of a Seven-marker Serum Protein Biosignature for the Diagnosis of Active TB disease in African Primary Health Care Clinic Attendees with Signs and Symptoms Suggestive of TB Talk presented at the South African Immunology Society Congress. GLENBURN LODGE & SPA, Johannesburg, South Africa, 06 March-09 November 2016.

Miller M. TB in Wildlife Talk presented at the International Conservation Workshop for Arabias Biodiversity. Conservation Workshop, Sharjah, United Arab Emirates, 08-11 February 2016.

Miller M. Tuberculosis in Under-Recognized Species – Is this an Emerging Disease Threat? Talk presented at the American Association of Zoo Veterinarians Annual Conference. Sheraton Hotel Conference Center, Atlanta, USA, 16-22 July 2016.

Miller M. Infectious Disease Threats to Wild Mammals in Southern Africa:  Risks at Intra- and Interspecies Interfaces Talk presented at the Mammal Research Institute 50th Anniversary Workshop. Mopani, Kruger National Park, Mopani, South Africa, 12-15 September 2016.

Miller M. IP-10 Enhances the Diagnosis of Bovine Tuberculosis in African Buffaloes Talk presented at the U.S. Animal Health Association Annual Conference. Greensboro Conference Center, Greensboro, USA, 15-20 October 2016.

Theron GDV, Reeve BW. How well do different diagnostic tests detect heteroresistance in tuberculosis patients? Talk presented at the Departmental Meeting. Lecture Hall 7, Cape Town, South Africa, 23 September 2016.

Mujuru TN. How well do different diagnostic tests detect heteroresistance in tuberculosis patients? Talk presented at the Departmental Meeting. Lecture Hall 7, Cape Town, South Africa, 23 September 2016.

Warren RM. The genetic basis of drug resistance in tuberculosis Talk presented at the Diagnosis and Management of Drug-Resistant Tuberculosis, ESCMID Postgraduate Technical Workshop. University of Cape Town Faculty of Health Sciences, Cape Town, South Africa, 18 January 2016.

Warren RM. Overview of drug resistant tuberculosis Talk presented at the Infectious Diseases Day. University of Pretoria, Pretoria, South Africa, 12 February 2016.

Warren RM. Drug Resistant Tuberculosis – lessons from South Africa Talk presented at the SACORE Meeting. Lilongwe, Lilongwe, Malawi, 01 March 2016.

Warren RM. Lights-on Light-off: clinical evaluation in South Africa Talk presented at the CPTR Workshop 2016. Washington, Washington, United States of America, 04-07 April 2016.

Warren RM. Biomolecular diagnostics, gene sequencing and whole genome sequencing Talk presented at the Tenth International Child TB Training Course. Goudini Spa, Cape Town, South Africa, 27 September 2016.

Haylett W, Neethling A. Exploring mitochondrial dysfunction in LRRK2 and Parkin cellular models of Parkinson’s disease Talk presented at the FMHS 60th Annual Academic Year Day. Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa, 01 August 2016.

Borrageiro G. Investigation of differential gene expression in Parkinson’s disease patients:A whole transcriptome approach Talk presented at the FMHS 60th Annual Academic Year Day. Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa, 11 August 2016.

Miller M. Evaluation of serum ferritin in free-ranging black rhinoceros as a tool to understand factors affecting iron overload disorder Talk presented at the 2nd international Workshop on Iron Overload Disorder in Browsing Rhinoceros. Disney’s Animal Kingdom, Orlando, USA, 21-22 January 2016.

Bibi Sebate. Targeted-resequencing of genes involved in neurological conitions in South African patients with Parkinson’s disease Talk presented at the Biomedical Sciences Research Day. Lecture Hall F139, Cape Town, South Africa, 15-23 November 2016.

Bardien S. Targeted-resequencing of genes involved in neurological conitions in South African patients with Parkinson’s disease Talk presented at the African Society of Human Genetics meeting. Dakar, Dakar, Senegal, 15-17 May 2016.

Bardien S. The genetics of Parkinson’s disease in South Africa Talk presented at the Congress of the Neurological Association of South Africa. Central Drakensberg, Kwa-Zulu Natal, South Africa, 15-20 March 2016.

Oluwole OG. A pilot study to evaluate a targeted resequencing approach for identification of pathogenic mutations in South African Parkinson’s disease patients Talk presented at the South African Association of Neuroscience Symposium. UCT Medical School University of Cape Town, Cape Town, South Africa, 09-11 December 2016.

Mouton JM, Elucidating population-wide mycobacterial replication dynamics at the single-cell level. The Acid-Fast Club, University of Cape Town, South Africa, May 2016, Oral Presentation.

Van Helden P, Drug Susceptibility Testing: Yesterday, Today and Tomorrow in the context of Heteroresistance. H3D Pioneering World-class Drug Discovery in Africa, 15-18 November 2016, Goudini Spa, Western Cape.

Posters (50) National and International

Masuku, B., Young, E., Mizrahi, V., Wilkinson, R. J., Mkhwanai, N., Koch, A. and Warner, D. F. Beyond the lab and behind the lens: An anthropological exploration of a youth-based community engagement initiative and its reflection of the lived experience of TB in Khayelitsha, Cape Town. TB2016, International AIDS Society, Durban, 16-17 July 2016

Sewgoolam, B. Koch, A., Mizrahi, V. & Warner, D.F. Investigating the fitness cost of rifampicin-resistance in mycobacteria. TB2016, International AIDS Society, Durban, 16-17 July 2016

Koch, A., Brites, D., Stucki, D., vans, J., Seldon, R., Nicol, M., Oni, T., Warner, D.F., Mizrahi, V., Parkhill, J., Gagneux, S., Marin, D., Wilkinson, R.J. The evolution of Mycobacterium tuberculosis in HIV co-infected individuals in an HIV/TB endemic setting. Poster presented at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

Sewgoolam, B., Koch, A., Mizrahi, V., Warner, D.F. Investigating the cost of rifampicin resistance in mycobacteria. Poster presented at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

Reiche, M., Martin, Z., Lang, D., Dhar, N., McKinney, J.D., Mizrahi, V., Warner, D.F. Recruitment and regulation of the mycobacterial mutasome. Poster presented at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

Ray, P., Boshoff, H., Arora, K., Tsang, P., Bayliss, N., Harrison, J., Murugesan, D., Buchanan, K., Green, S., Zucotto, F., Read, K., Scullion, P., Epemolu, R., McKenzie, C., Mizrahi V., Warner, D.F., Barry, C., Wyatt, P. Poster presented at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

Van der Westhuyzen, R., Moosa, A., Naran, K., Warner, D.F., Chibale, K. Pyrrolo-[3,4-c]-pyridine-1,3(2H)-diones: a novel antimycoacterial class targeting mycobacterial respiration. Poster presented at the EMBO Conference on Tuberculosis 2016: Interdisciplinary research on tuberculosis and pathogenic mycobacteria, Institut Pasteur, Paris, 19-23 September 2016

van Coller, S.J., Ioerger, T. R., Mizrahi, V., Warner, D.F. DnaE2 plays no role in starvation-induced adaptive mutagenesis in Mycobacterium tuberculosis. DNA polymerase meeting: from molecular function to human diseases, Biarritz, France, 4–8 October 2016

Agarwal, P., and Mizrahi, V. Growth, persistence and drug susceptibility of Mycobacterium tuberculosis in foamy macrophages: implications for TB drug discovery. Poster presented at Metchnikoff’s Legacy: Tissue Phagocytes and Function. Napoli, Italy 12-14 October 2016

Morrow, C., Bryden, W., Silcott, D., Warner, D.F., Scriba, T., Blackburn, J., Mizrahi, V., and Wood, R. Real-time investigation of tuberculosis transmission. Poster presented at the 47th Union World Conference on Lung Health, Liverpool, 24-26 October 2016

Omollo, C; Chibale,K, Warner D.  Developing synergistic combinations to restore sensitivity in drug-resistant Mycobacterium tuberculosis. Poster presentation at a Drug discovery Symposium on Tuberculosis and Neglected Diseases, Goudini Spa Resort, Cape Town, 15-18 November 2016

Sikhosana. N., Chengalroyen M. and Kana. B. Mycobacterium amidases: Biological function and putative role in cell wall remodelling. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Papadopoulos. A. and Kana. B. Characterisation Of M23-Domain Activators of Peptidoglycan Degrading Amidases in Mycobacterium tuberculosis. Poster presentation at the 7th Cross-faculty Postgraduate Symposium, University of the Witwatersrand. 1-2 March 2016

Papadopoulos. A. and Kana. B   A novel family of proteins in Mycobacterium tuberculosis with putative, differential roles in bacterial cell wall degradation. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

McIvor. A., Peters. J., Papadopoulos. A., Gordhan. B., Martinson. M., Letutu. M. and Kana. B.D. Decline in non-culturable tubercle bacteria in HIV-positive and HIV-negative tuberculosis patients during early first-line treatment Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

McIvor. A., Peters. J., Papadopoulos. A., Gordhan. B., Martinson. M., Letutu. M. and Kana. B.D. Decline in non-culturable tubercle bacteria in HIV-positive and HIV-negative tuberculosis patients during early first-line treatment. Poster presentation at the Molecular Biosciences Research Thrust Postgraduate Research Day. 8 December 2016. Won second prize for best poster presentation.

Ralefeta D., Machowski E. and Kana B.D. Mycobacterial DD-CArboxypetidases: Filling In The Gaps. Poster presentation at the Wits 7th Cross Faculty Symposium. 1 September 2016

Mashilo. P. and Kana. B.D. Characterization of mycobacterial peptidoglycan remodelling enzymes. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Mashilo. P. and Kana. B.D. Characterization of mycobacterial peptidoglycan remodelling enzymes. Poster presentation at the Molecular Biosciences Research Thrust Research Day. 8 December 2016

Maphatsoe. M., Ealand. C., and Kana. B.D. Cellular localization of low molecular weight penicillin binding proteins and other cell wall remodelling enzymes in Mycobacterium smegmatis. Poster presentation at the Biennial Research Day and Postgraduate Expo, Faculty of Health Sciences. 01 September 2016

Chengalroyen M., Beukes G., Gordhan B., Martinson N., Otwombe K. and Kana B.D. The detection and quantification of differentially culturable tubercle bacteria in sputum from tuberculosis patients. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Narrandes. N. and Kana. B.D. Characterization of the mycobacterial electron transport chain. Poster presentation at the MBRT Postgraduate Research Day. Wits Club, West Campus, university of the Witwatersrand.  8 December 2016. Won third prize for best poster presentation.

Mashigo. L., Ealand. C. and Kana. B.D. DacB is essential in Mycobacterium smegmatis. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

Manamela. N., Kana. B.D. and Gordhan. B. DNA repair and mutagenesis in Mycobacterium smegmatis. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Expo. 1 September 2016

du Plessis N, Jacobs R, Fang Z, van Helden P, Lutz M, Walzl G. TUBERCULOSIS: Investigations into the role of MDSC Poster presented at the Regulatory Myeloid Suppressor Cells:  From Basic Discovery to Therapeutic Application. Wistar Institute, Philadelphia, USA, 23-26 June 2016.

Kleynhans L, Tshivhula H, Kunsevi C, Ronacher K. Changes in Endocrine and Immune Signatures during TB treatment Poster presented at the Keystone Symposium: B6, Tuberculosis Co-Morbidities and Immunopathogenesis. Keystone Resort, Colorado, USA, 28 February- 03 March 2016.

Niemand N, Weber B, Williams MJ. Investigating the function of A-type carrier proteins in mycobacteria Poster presented at the South African Society of Biochemistry and Molecular Biology (SASBMB). East London ICC, East London, South Africa, 10-13 July 2016.

Loxton AG. The Functional Response of B cells to Antigenic Stimulation during Latent Tuberculosis Poster presented at the International Conference of Immunology 2016. Melbourne Convention Centre, Melbourne, Australia, 21 August-27 November 2016.

Loxton AG, van Rensburg I. The Functional Response of B cells to Antigenic Stimulation during Latent Tuberculosis Poster presented at the B Cells at the Intersection of Innate and Adaptive Immunity (E3) - Keystone Symposia. Clarion Hotel Sign, Stockholm, Sweden, 29 May- 02 June 2016.

Salie M, Schurz H, van Helden PD, Möller M, Hoal EGl. Genetic contributions to the MHC and LRC regions of a South African population and its effect on TB vaccine efficacy Poster presented at the The American Society of Human Genetics 66th Annual Meeting. Vancouver Convention Centre, Vancouver, Canada, 18-22 October 2016.

Kreiswirth B, Rice J, Wangh L, Whitfield MG, Warren RM, Posey J, Bifani P, Marras S. Virtual sequencing of the entire pncA gene target in a single tube using Late-PCR and Lights-On/Lights-Off probes to predict PZA susceptibility Poster presented at the 37th Annual Congress of the European Society of Mycobacteriology. Sheraton Catania Hotel & Conference Center, Catania, Sicily, Italy, 03-06 July 2016.

Sylvester TT, van Helden P, Miller MA, Parsons SDC, Loxton AG. Screening of commercially available antibodies for flow cytometric analysis in African lions (Panthera leo) : A pilot study Poster presented at the FMHS 60th Annual Academic Year Day. Teaching Building, Tygerberg Campus, Stellenbosch University, Cape Town, South Africa, 11 August 2016.

Willemse D, Warren RM, Williams MJ. Truncation of Rv1460 increases sensitivity of Mycobacterium tuberculosis to oxidative stress Poster presented at the H3D Symposium. Goudini Spa, Cape Town, South Africa, 15-18 November 2016.

Malherbe ST, Ronacher K, Warwick J, Walzl G. Tuberculosis treatment response with 18F-FDG-PET/CT imaging Poster presented at the Western Cape Provincial Health Research Day. SUN Faculty of Medicine Health Sciences, Tygerberg, Cape Town, South Africa, 04 November 2016.

Werely C, Cloete R, Akurugu W, van Helden P, Christoffels A. Modelling the Structural and functional effects of nucleotide polymorphisms in the NAT1 drug metabolising enzyme. Poster presented at the 8th Santorini Conference Biology Prospective: Systems Medicine, Personalised Health and Therapy.  European Society of Pharmacogenomics and Personalised Therapy. Petros M. Nomikos Conference Centre, Thira, Santorini Island, Greece, 03-05 October 2016.

Naidoo CC. Extensively drug-resistant M. tuberculosis strains of the F15/LAM4/KZN genotype exhibit enhanced proinflammatory cytokine induction in THP-1 macrophages Poster presented at the 2016 TB SUMMIT. The ICC, Durban, South Africa, 16-17 July 2016.

Venter R, Derendinger B, de Vos M, Naidoo S, van Helden PD, Warren R, Theron, G. DNA extracted from used Xpert MTB/RIF cartridges can be used for second-line drug susceptibility testing. Poster presented at the 2016 TB SUMMIT. The ICC, Durban, South Africa, 17 July 2016.

Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden P, Wiid I, Baker B. The Host Response to a Clinical MDR Mycobacterial Strain Cultured in a Detergent-Free Environment: A Global Transcriptomics Approach Poster presented at the Tuberculosis Co-Morbidities And Immunopathogenesis. Keystone, Colorado, USA, 29 February-03 March 2016.

Sao Emani C, Williams M, Wiid I, Van Helden P, Baker B. The role of ergothioneine in the physiology of Mycobacterium smegmatis. Poster presented at the Keystone Symposia Conference: Tuberculosis Co-Morbidities and Immunopathogenesis, Keystone, Colorado, USA, 28 February – 03 March 2016.

Derendinger B, de Vos M, van Helden PD, Warren RM, Theron G. Decreased sensitivity and increased indeterminate rates of GenoType MTBDRplus (v2.0) are associated with use of inappropriate PCR ramp rate: Implications for routine diagnostic laboratories Poster presented at the 2016 TB SUMMIT. Durban International Conference Centre, Durban, South Africa, 16-17 July 2016.

Pule C. Underlying physiological changes of drug resistant Mycobacterium tuberculosis Poster presented at the 2016 TB SUMMIT. Cineworld, Greenwich, London, UK, 21-23 June 2016.

Jacobs R, Walzl G, Chegou NN. Evaluation of novel host markers detected in plasma and saliva as biosignatures for the rapid diagnosis of TB disease and monitoring of the response to TB treatment Poster presented at the 5th South African Society for Immunology Congress. GLENBURN LODGE & SPA, Johannesburg, South Africa, 06-09 March 2016.

Jacobs R, Walzl G, Chegou NN. Identification of novel tuberculosis diagnostic biomarkers in plasma and saliva Poster presented at the European Respiratory Society International Congress 2016. London ExCel, London, United Kindgom, 03-07 September 2016.

Jacobs R, Walzl G, Chegou NN. Identification of novel plasma and salivary biosignatures for the diagnosis of TB disease and monitoring of treatment response Poster presented at the 8th EDCTP Forum. New Government Complex, Lusaka, Zambia, 06-09 November 2016.

Haylett W. ATPAF1 and SEPT9 are novel protein substrates of Parkin Poster presented at the 11th Annual Meeting of the Genetic Epidemiology of Parkinson's Disease Consortium and the 3rd International Parkinson's Disease Symposium. Luxembourg Centre for Systems Biomedicine, University of Louxembourg, Esch-Sur-Alzette, Luxembourg, 05-08 October 2016.

Borrageiro G. Investigation into the genetic etiology in South Africa Parkinson's disease patients Poster presented at the 20th International Congress of Parkinson's Disease and Movement Disorders. City Cube, Berlin, Germany, 19-23 June 2016.

Oluwole OG. A pilot study to evaluate a targeted resequencing approach for identification of pathogenic mutations in South African Parkinson's disease patients Poster presented at the FMHS 60th Annual Academic Year Day. Faculty of Medicine and Health Sciences, Tygerberg Campus, SU, Cape Town, South Africa, 11 August 2016.

Oluwole OG. A pilot study to evaluate a targeted resequencing approach for identification of pathogenic mutations in South African Parkinson's disease patients Poster presented at the Biomedical Science Research Day. Faculthy of medicine and Health Sciences, Tygerberg campus, SU, Cape Town, South Africa, 23 November 2016.

Willemse D., Weber B., Warren R.W., Williams M.J. Rv1460 functions as a repressor of the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon in Mycobacterium tuberculosis (Student poster presentation, Keystone Symposium, New Developments in Our Basic Understanding of Tuberculosis (A5), Vancouver, British Columbia Canada January 14—18, 2017).

Baatjies L., Niemand N. and Williams M.J. Investigating the susceptibility of a Mycobacterium smegmatis MSMEG_4272 conditional mutant to various compounds. (Student poster presentation, H3D Symposium 2016, Goudini Spa, Western Cape, 15 – 18 November 2016).


Products / Artifacts / Patents

Title: Host biomarkers for immunodiagnosis and monitoring of tuberculosis disease. Inventors: Jacobs, R, Walzl, G, Chegou, NN,. Applicant: Stellenbosch University, Application type: Provisional patent application, Country: South Africa, Application: ZA 2016/02557, Filing date: 2016/04/15, Status: Expired, PCT Filed;

Same patent: Country: PCT/WIPO, Application number: PCT/IB2017/052142, Filing date: 13/04/2017, Status: Pending

2015

* The Names in bold are CBTBR staff and students
Books / Chapters in Books (Total: 1)

  • Walzl G, Haks MC, Joosten SA, Kleynhans L, Ronacher K, Ottenhoff THM. Clinical immunology and multiplex biomarkers of human tuberculosis. In: Tuberculosis. Editors Kaufmann SHE, Rubin EJ, Zumla A. Cold Spring Harbour Laboratory Press, New York, 2015. P 197-209.

Articles in Peer-Reviewed Journals (Total: 84)

  • Hassim F, Papadopoulos AO, Kana BD, Gordhan BG. (2015) A combinatorial role for MutY and Fpg DNA glycosylases in mutation avoidance in Mycobacterium smegmatis. Mutat Res. 779: 24-32. (IF=3.680)
  • Narrandes NC, Machowski EE, Mizrahi V, Kana BD. (2015) Cleavage of the moaX-encoded fused molybdopterin synthase from Mycobacterium tuberculosis is necessary for activity. BMC Microbiol. 15:22. (IF=2.729)
  • Williams MJ, Shanley CA, Zilavy A, Peixoto B, Manca C, Kaplan G, Orme IM, Mizrahi V, Kana BD. (2015) bis-Molybdopterin guanine dinucleotide is required for persistence of Mycobacterium tuberculosis in guinea pigs. Infect Immun. 83: 544-550. (IF=3.731)
  • Lebina L, Abraham PM, Motlhaoleng K, Rakgokong M, Variava E, Martinson NA. (2015) The use of decentralized GeneXpert by trained non-laboratory technicians in rural clinics in South Africa. Tuberculosis (Edinb). 95: 625-626. (IF=2.711)
  • Lala SG, Little KM, Tshabangu N, Moore DP, Msandiwa R, van der Watt M, Chaisson RE, Martinson NA. (2015) Integrated Source Case Investigation for Tuberculosis (TB) and HIV in the Caregivers and Household Contacts of Hospitalised Young Children Diagnosed with TB in South Africa: An Observational Study. PLoS One 10:e0137518. (IF=3.234)
  • Lebina L, Abraham PM, Milovanovic M, Motlhaoleng K, Chaisson RE, Rakgokong M, Golub J, Variava E, Martinson NA. (2015) Latent tuberculous infection in schoolchildren and contact tracing in Matlosana, North West Province, South Africa. Int J Tuberc Lung Dis. 19:1290-1292. (IF=2.315)
  • Omar T, Variava E, Moroe E, Billioux A, Chaisson RE, Lebina L, Martinson NA. (2015) Undiagnosed TB in adults dying at home from natural causes in a high TB burden setting: a post-mortem study. Int J Tuberc Lung Dis. 19:1320-1325. (IF=2.315)
  • Singh V, Brecik M, Mukherjee R, Evans JC, Svetlíková S, Blaško J, Blackburn J, Warner DF, Mikušová K, Mizrahi V. (2015) The complex mechanism of antimycobacterial action of 5-fluorouracil. Chem. Biol. 22:63-75. (IF=6.645)
  • Gopinath K, Warner DF, Mizrahi V. (2015) Targeted gene knockout and essentiality testing by homologous recombination. Methods Mol Biol. 1285:131-149. (IF=1.290)
  • Evans JC, Mizrahi V. (2015) The application of tetracycline regulated gene expression systems in the validation of novel drug targets in Mycobacterium tuberculosis. Front Microbiol. 6:812. (IF=3.989)
  • van der Westhuyzen R, Winks S, Wilson CR, Boyle GA, Gessner RK, Soares de Melo C, Taylor D, de Kock C, Njoroge M, Brunschwig C, Lawrence N, Rao SP, Sirgel F, van Helden PD, Seldon R, Moosa A, Warner DF, Arista L, Manjunatha UH, Smith PW, Street LJ, Chibale K. (2015) Pyrrolo[3,4-c]pyridine-1,3(2H)-diones: A Novel Antimycobacterial Class Targeting Mycobacterial Respiration. J. Med. Chem. 58(23): 9371-9381. (IF=5.447)
  • Soares de Melo C, Feng TS, van der Westhuyzen R, Gessner RK, Street LJ, Morgans GL, Warner DF, Moosa A, Naran K, Lawrence N, Boshoff HI, Barry CE 3rd, Harris CJ, Gordon R, Chibale K. (2015) Aminopyrazolo [1,5-a]pyrimidines as potential inhibitors of Mycobacterium tuberculosis: Structure activity relationships and ADME characterization. Bioorg Med Chem. 23(22):7240-7250. (IF=2.420)
  • Warner DF, Koch A, Mizrahi V. (2015) Diversity and disease pathogenesis in Mycobacterium tuberculosis. Trends Microbiol. 23(1):14-21. (IF=9.186)
  • Jones CE, Hesseling AC, Tena-Coki NG, Scriba TJ, Chegou NN, Kidd M, Wilkinson RJ, Kampmann B. (2015) The impact of HIV exposure and maternal Mycobacterium tuberculosis infection on infant immune responses to Bacille Calmette-Guérin vaccination. AIDS. 29(2):155-165. (IF=5.554)
  • Chigutsa E, Pasipanodya JG, Visser ME, van Helden PD, Smith PJ, Sirgel FA, Gumbo T, McIlleron H. (2015) The impact of non-linear interactions of pharmacokinetics and MICs on sputum bacillary kill rates as a marker of sterilizing effect in tuberculosis. AAC. 59(1):38-45. (IF=4.476)
  • Visser DH, Solomons RS, Ronacher K, van Well GT, Heymans MW, Walzl G, Chegou NN, Schoeman JF, van Furth AM. (2015) The host immune response to tuberculous meningitis. Clinical Infectious Diseases. 60(2):177-187. (IF=8.886)
  • Fang Z, van der Merwe RG, Warren RM, Schubert W-D, Gey van Pittius NC. (2015) Assessing the progress of Mycobacterium tuberculosis H37Rv structural genomics. Tuberculosis. 95(2):131–136. (IF=2.711)
  • Fang Z, Sampson SL, Warren RM, Gey van Pittius NC, Newton-Foot M. (2015) Iron acquisition strategies in mycobacteria. Tuberculosis. 95(2):123–130. (IF=2.711)
  • Ronacher K, Joosten S, van Crevel R, Dockrell H, Walzl G, Ottenhoff T. (2015) Acquired immunodeficiencies and tuberculosis: Focus on HIV/AIDS and Diabetes Mellitus. Immunological Reviews. 264(1):121-137. (IF=10.120)
  • Mbugi E, Katale B, Siame K, Keyyu J, Kendall S, Dockrell H, Streicher E, Michel A, Rweyemamu M, Warren R, Matee M, van Helden P. (2015) Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania. Tuberculosis. 95(2):170-178. (IF=2.711)
  • Miller M, Buss P, Hofmeyr J, Olea-Popelka F, Parsons S, van Helden P. (2015) Antemortem Diagnosis of Mycobacterium bovis Infection in Free-Ranging African Lions (Panthera leo) and Implications for Transmission. Journal of Wildlife Disease. 51(2): 493-497. (IF=1.355)
  • Le Roex N, Cooper D, van Helden PD, Hoal EG, Jolles AE. (2015) Disease Control in Wildlife: Evaluating a Test and Cull Programme for Bovine Tuberculosis in African Buffalo. Transbound Emerg Dis. (Epub Jan 2015). (IF=2.944)
  • Merker M, Blin C, Mona S, Duforet-Frebourg N, Lecher S, Willery E, Blum M, Rüsch-Gerdes S, Mokrousov I, Aleksic E, Allix-Béguec C, Antierens A, Augustynowicz-Kopeć E, Ballif M, Barletta F, Beck HP, Barry CE 3rd, Bonnet M, Borroni E, Campos-Herrero I, Cirillo D, Cox H, Crowe S, Crudu V, Diel R, Drobniewski F, Fauville-Dufaux M, Gagneux S, Ghebremichael S, Hanekom M, Hoffner S, Jiao WW, Kalon S, Kohl TA, Kontsevaya I, Lillebæk T, Maeda S, Nikolayevskyy V, Rasmussen M, Rastogi N, Samper S, Sanchez-Padilla E, Savic B, Shamputa IC, Shen A, Sng LH, Stakenas P, Toit K, Varaine F, Vukovic D, Wahl C, Warren RM, Supply P, Niemann S, Wirth T. (2015) Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage. Nature Genet. 47(3):242-249. (IF=29.352)
  • Mikota SK, Gairhe K, Giri K, Hamilton K, Miller M, Paudel S, Lyashchenko K, Larsen RS, Payer JB, Waters R, Greenwald R, Dumonceaux G, Vincent B, Kaufman GE. (2015) Tuberculosis surveillance of elephants (Elephas maximus) in Nepal at the captive-wild interface. European Journal of Wildlife Research. 61(2): 221-229. (IF=1.634)
  • Dippenaar A, Warren RM. (2015) Tuberculosis: Fighting an old disease with next-generation sequencing. eLife. 4:e06782. (IF=9.322)
  • Miller MA, Greenwald R, Lyashchenko KP. (2015) Serodiagnosis of TB in black rhinoceros. Journal of Zoo and Wildlife Medicine. 46(1): 100–104. (IF=0.424)
  • Locher CP, Jones SM, Hanzelka BL, Perola E, Shoen CM, Cynamon MH, Ngwane AH, Wiid IJ, van Helden PD, Betoudji F, Nuermberger EL, Thomson JA. (2015) A Novel Inhibitor of Gyrase B is a Potent Drug Candidate for the Treatment of Tuberculosis and Non-Tuberculosis Mycobacteria. Antimicrob Agents Chemother. 59(3):1455-1465. (IF=4.476)
  • Farhat MR, Mitnick CD, Franke MF, Kaur D, Sloutsky A, Murray M, Jacobson KR. (2015) Concordance of Mycobacterium tuberculosis fluoroquinolone resistance testing: implications for treatment. Int J Tuberc Lung Dis. 19(3):339-341. (IF=2.315)
  • Fishbein S, van Wyk N, Warren RM, Sampson SL. (2015) Phylogeny to function: PE/PPE protein evolution and impact on Mycobacterium tuberculosis pathogenicity. Mol Microbiol. 96(5):901-916. (IF=4.419)
  • Fortuin S, Tomazella G, Nagayaryan N, Sampson S, Gey van Pittius N, Da Cruz Soares N, Wiker H, De Souza G, Warren RM. (2015) Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: Expanding the mycobacterial phosphoproteome catalogue. Front. Microbiol. 6:6. (IF=3.989)
  • Mandalakas AM, Kirchner HL, Walzl G, Gie RP, Schaaf HS, Cotton MF, Grewal HM, Hesseling AC. (2015) Optimizing the Detection of Recent Tuberculosis Infection in Children in a High TB-HIV Burden Setting. Am J Respir Crit Care Med. 191(7):820-830. (IF=12.996)
  • Daya M, van der Merwe L, van Helden PD, Möller M, Hoal EG. (2015) Investigating the Role of Gene-Gene Interactions in TB Susceptibility. PloS One. 10(4):e0123970. (IF=3.234)
  • Masson L, Passmore JA, Liebenberg LJ, Werner L, Baxter C, Arnold KB, Williamson C, Little F, Mansoor LE, Naranbhai V, Lauffenburger DA, Ronacher K, Walzl G, Garrett NJ, Williams BL, Couto-Rodriguez M, Hornig M, Lipkin WI, Grobler A, Karim QA, Karim SS. (2015) Genital Inflammation and the Risk of HIV Acquisition in Women. Clin Infect Dis. 61(2):260-269. (IF=8.886)
  • Mutai P, Pavadai E, Wiid I, Ngwane A, Baker B, Chibale K. (2015) Synthesis, antimycobacterial evaluation and pharmacophore modeling of analogues of the natural product formononetin. Bioorganic & Medicinal Chemistry Letters. 25:2510–2513. (IF=2.420)
  • van Rensburg IC, Loxton AG. (2015) Transcriptomics: the key to biomarker discovery during tuberculosis? Biomark Med. 9(5):483-495. (IF=2.646)
  • Axelsson-Robertson R, Rao M, Loxton AG, Walzl G, Bates M, Zumla A, Maeurer M. (2015) Frequency of Mycobacterium tuberculosis-specific CD8+ T-cells in the course of anti-tuberculosis treatment. Int J Infect Dis. 32:23-29. (IF=1.859)
  • Hesseling AC, Jaspan HB, Black GF, Nene N, Walzl G. (2015) Immunogenicity of BCG in HIV-exposed and non-exposed infants following routine birth or delayed vaccination. Int J Tuberc Lung Dis. 19(4):454-462. (IF=2.315)
  • Pietersen E, Peter J, Streicher E, Sirgel F, Rockwood N, Mastrapa B, Te Riele J, Davids M, van Helden P, Warren R, Dheda K. (2015) High frequency of resistance, lack of clinical benefit, and poor outcomes in capreomycin treated South african patients with extensively drug-resistant tuberculosis. PloS One. 10(4):e0123655. (IF=3.234)
  • Landolfi J, Terio K, Miller M, Junecko B, Reinhart T. (2015) Pulmonary Tuberculosis in Asian Elephants (Elephas maximus): Histologic Lesions With Correlation to Local Immune Responses. Vet Pathology. 52(3):535-542. (IF=1.869)
  • Viljoen IM, van Helden PD, Millar RP. (2015) Mycobacterium bovis infection in the lion (Panthera leo): Current knowledge, conundrums and research challenges. Veterinary Microbiology. 177(3-4):252-260. (IF=2.511)
  • Hamasur B, Bruchfeld J, van Helden PD, Källenius G, Svenson S. (2015) A sensitive urinary lipoarabinomannan test for tuberculosis in HIV negative patients. Plos ONE. 10(4):e0123457. (IF=3.234)
  • Buss P, Miller M, Hofmeyr J, Mathebula N, Kruger M, Bruns A, Martin L, Olea-Popelka F. (2015) Evaluation of cardiorespiratory, blood gas, and lactate values during extended immobilization of white rhinoceros (Ceratotherium simum). J Zoo and Wildlife Medicine. 46(2):224-233. (IF=0.424)
  • Sy S, de Kock L, Diacon A, Werely CJ, Xia H, Rosenkranz B, van der Merwe L, Donald PR. (2015) N-Acetyltransferase genotypes and the pharmacokinetics and tolerability of para-aminosalicylic acid in patients with drug-resistant pulmonary tuberculosis. AAC. 59(7):4129-4138. (IF= 4.476)
  • Van Halsema CL, Chihota VN, Gey van Pittius NC, Fielding KL, Lewis JJ, van Helden PD, Churchyard GJ, Grant AD. (2015) Clinical relevance of non-tuberculous mycobacteria isolated from sputum in a gold mining workforce in South Africa: an observational, clinical study. BioMed Research International. 959107:1-10. (IF=3.169)
  • le Roex N, Berrington CM, Hoal EG, van Helden PD. (2015) Selective breeding: The future of TB management in African buffalo? Acta Tropica. 149:38-44. (IF=2.270)
  • Cliff JM, Kaufmann SH, McShane H, van Helden P, O'Garra A. (2015) The human immune response to tuberculosis and its treatment: a view from the blood. Immunol Rev. 264(1):88-102. (IF=10.120)
  • Baumann R, Kaempfer S, Chegou NN, Oehlmann W, Spallek R, Loxton AG, van Helden PD, Black GF, Singh M, Walzl G. (2015) A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens. Mediators of Inflammation. e364758.  (IF=3.236)
  • Cliff JM, Cho JE, Lee JS, Ronacher K, King EC, van Helden P, Walzl G, Dockrell HM. (2016) Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. J Infect Dis. 213(3):485-495. (IF=5.997)
  • Whitfield MG, Soeters HM, Warren RM, York T, Sampson SL, Streicher EM, van Helden PD, van Rie A. (2015) A Global Perspective on Pyrazinamide Resistance: Systematic Review and Meta-analysis. PloS One. 10(7):e0133869. (IF=3.234)
  • Whitfield MG, Warren RM, Streicher EM, Sampson SL, Sirgel FA, van Helden PD, Mercante A, Willby M, Hughes K, Birkness K, Morlock G, van Rie A, Posey JE. (2015) Mycobacterium tuberculosis pncA polymorphisms that do not confer pyrazinamide resistance at a breakpoint concentration of 100ug/ml in MGIT. JCM. 53(11):3633-3635. (IF=3.993)
  • Kayigire XA, Friedrich SO, van der Merwe L, Donald PR, Diacon AH. (2015) Simultaneous staining of sputum smears for acid-fast and lipid-containing Myobacterium tuberculosis can enhance the clinical evaluation of antituberculosis treatments. Tuberculosis. 95(6):770-779. (IF=2.711)
  • Masson L, Salkinder AL, Olivier AJ, McKinnon LR, Gamieldien H, Mlisana K, Scriba TJ, Lewis DA, Little F, Jaspan HB, Ronacher K, Denny L, Abdool Karim SS, Passmore JS. (2015) Relationship between female genital tract infections, mucosal IL-17 production and local Th17 cells. Immunology. 146(4):557-567. (IF=3.795)                              
  • Goosen WJ, Cooper D, Miller MA, van Helden PD, Parsons SD. (2015) IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer). Clin Vaccine Immunol. 22(8):974-978. (IF=2.470)
  • Haw A, Hofmeyr M, Buss P, Miller M, Meyer L, Fuller A, Fleming G. (2015) Evaluation of butorphanol combined with oxygen insufflation to improve cardiorespiratory function during chemical immobilization of free-ranging white rhinoceros (Ceratotherium simum).  J. S. Afr. Vet. Assoc.  86(1):1-10 pages. (IF= 0.350)
  • Miller M, Buss P, Olea-Popelka F, Wanty R, Parsons S, van Helden P. (2015) Baseline hematologic parameters for free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.  J. Wildl. Dis.  doi:10.7589/2015-03-081. (IF=1.355)
  • Streicher EM, Sampson SL, Dheda K, Dolby T, Simpson JA, Victor TC, Gey van Pittius NC, van Helden PD, Warren RM. (2015) Molecular epidemiological interpretation of the extensively drug resistant tuberculosis epidemic in South Africa. JCM. 53(11):3650-3653. (IF=3.993)
  • Corstjens PL, Tjon Kon Fat EM, de Dood CJ, van der Ploeg-van Schip JJ, Franken KL, Chegou NN, Sutherland JS, Howe R, Mihret A, Kassa D, van der Vyver M, Sheehama J, Simukonda F, Mayanja-Kizza H, Ottenhoff TH, Walzl G, Geluk A; AE-TBC consortium. (2016) Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa. Clin Biochem. 49(1):22-31. (IF=2.275)
  • Salie M, Daya M, Lucas LA, Warren RM, van der Spuy GD, van Helden PD, Hoal EG, Möller M. (2015) Association of toll-like receptors with susceptibility to tuberculosis suggests sex-specific effects of TLR8 polymorphisms. Infect Genet Evol. 34:221-229. (IF=3.015)
  • Torres JN, Paul LV, Rodwell TC, Victor TC, Amallraja AM, Elghraoui A, Goodmanson AP, Ramirez-Busby SM, Chawla A, Zadorozhny V, Streicher EM, Sirgel FA, Catanzaro D, Rodrigues C, Gler MT, Crudu V, Catanzaro A, Valafar F. (2015) Novel katG mutations causing isoniazid resistance in clinical M. tuberculosis isolates. Emerg Microbes Infect. 4(7):e42. (IF=2.260)
  • Van Rie A, Warren RM. (2015) MDR tuberculosis control: time to change the dogma? Lancet. 3(12):907-909. (IF=45.217)
  • Losi M, Knights AJ, Mariani F, Altieri AM, Paone G, Loxton AG, Chegou NN, Kenneth J, Alma MG, Colizzi V, Walzl G, Saltini C, Boyle J, Amicosante M. (2016) QuantiFERON-TB performance enhanced by novel Mycobacterium tuberculosis-specific antigens. Europ Resp Journal. 47(2):660-664. (IF=7.636)
  • Theron G, Jenkins HE, Cobelens F, Abubakar I, Khan AJ, Cohen T, Dowdy DW. (2015) Data for action: collection and use of local data to end tuberculosis. Lancet. 386(10010):2324-2333. (IF=45.217)
  • van der Westhuyzen R, Winks S, Wilson CR, Boyle GA, Gessner RK, Soares de Melo C, Taylor D, de Kock C, Njoroge M, Brunschwig C, Lawrence N, Rao SP, Sirgel F, van Helden PD, Seldon R, Moosa A, Warner DF, Arista L, Manjunatha UH, Smith PW, Street LJ, Chibale K. (2015) Pyrrolo[3,4-c]pyridine-1,3(2H)-diones: A Novel Antimycobacterial Class Targeting Mycobacterial Respiration. J Med Chem. 58(23):9371-81. (IF=5.447)
  • Hammond-Aryee K, van Helden LS, van Helden PD. (2015) The prevalence of antibodies to Toxoplasma gondii in sheep in the Western Cape, South Africa. OVJ. 82(1):E1-5. (IF=1.256)
  • Dippenaar A, Parsons SD, Sampson SL, van der Merwe RG, Drewe JA, Abdallah AM, Siame KK, Gey van Pittius NC, van Helden PD, Pain A, Warren RM. (2015) Whole genome sequence analysis of Mycobacterium suricattae. Tuberculosis. 95(6):682-688. (IF=2.711)
  • Black PA, de Vos M, Louw GE, van der Merwe RG, Dippenaar A, Streicher EM, Abdallah AM, Sampson SL, Victor TC, Dolby T, Simpson JA, van Helden PD, Warren RM, Pain A. (2015) Whole genome sequencing reveals genomic heterogeneity and antibiotic purification in Mycobacterium tuberculosis isolates. BMC Genomics. 16(1):857. (IF=3.986)
  • Pule CM, Sampson SL, Warren RM, Black PA, van Helden PD, Victor TC, and Louw GE. (2016) Efflux pump inhibitors: targeting mycobacterial efflux systems to enhance TB therapy. J Antimicrob Chemother. 71(1):17-26. (IF=5.313)
  • Aas M, Blokland GA, Chawner SJ, Choi SW, Estrada J, Forsingdal A, Friedrich M, Ganesham S, Hall L, Haslinger D, Huckins L, Loken E, Malan-Müller S, Martin J, Misiewicz Z, Pagliaroli L, Pardiñas AF, Pisanu C, Quadri G, Santoro ML, Shaw AD, Ranlund S, Song J, Tesli M, Tropeano M, van der Voet M, Wolfe K, Cormack FK, DeLisi L. (2016) Summaries of plenary, symposia, and oral sessions at the XXII World Congress of Psychiatric Genetics, Copenhagen, Denmark, 12-16 October 2014. Psychiatr Genet. 26(1):1-47. (IF=1.941)
  • Esterhuyse MM, Weiner J 3rd, Caron E, Loxton AG, Iannaccone M, Wagman C, Saikali P, Stanley K, Wolski WE, Mollenkopf HJ, Schick M, Aebersold R, Linhart H, Walzl G, Kaufmann SH. (2015) Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers. mBIO. 6(5):e01187-15. (IF=6.786)
  • Nicol MP, Gnanashanmugam D, Browning R, Click ES, Cuevas LE, Detjen A, Graham SM, Levin M, Makhene M, Nahid P, Perez-Velez CM, Reither K, Song R, Spiegel HM,Worrell C, Zar HJ, Walzl G. (2015) A Blueprint to Address Research Gaps in the Development of Biomarkers for Pediatric Tuberculosis. Clin Infect Dis. 61(suppl 3): S164-S172. (IF=8.886)
  • Kriel M, Lotz JW, Kidd M, Walzl G. (2015) Evaluation of a radiological severity score to predict treatment outcome in adults with pulmonary tuberculosis. Int J Tuberc Lung Dis. 19(11):1354-1360. (IF=2.315)
  • Schurz H, Daya M, Möller M, Hoal EG, Salie M. (2015) TLR1, 2, 4, 6 and 9 variants associated with tuberculosis susceptibility: a systematic review and meta-analysis. Plos One. 10(10):e0139711. (IF=3.234)
  • Theron G. (2015) How should laboratories measure bacterial load? S Afr Resp J. 21(3):54. (IF=Unknown)
  • Macuamule CL, Wiid IJ, van Helden PD, Tanner M, and Witthuhn RC. (2016) Effect of milk fermentation by kefir grains and selected single strains of lactic acid bacteria on the survival of Mycobacterium bovis BCG. Int J Food Microbiol. 217:170-176. (IF=3.082)
  • ElMaraachli W, Slater M, Berrada ZL, Lin SY, Catanzaro A, Desmond E, Rodrigues C, Victor TC, Crudu V, Gler MT, Rodwell TC. (2015) Predicting differential rifamycin resistance in clinical Mycobacterium tuberculosis isolates by specific rpoB mutations. Int J Tuberc Lung Dis. 19(10):1222-1226. (IF=2.315)
  • Miller M, Buss P, Wanty R, Parsons S, van Helden P, Olea-Popelka F. (2015) Baseline hematologic results for free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.  J Wildl Dis.  51(4):916-922. (IF=1.355)
  • Ticha LA, Klaasen JA, Green IR, Naidoo S, Baker B, Pietersen RD. (2015) Phytochemical and Antimicrobial Screening of Flavanones and Chalcones from Galenia africana and Dicerothamnus rhinocerotis. Nat Prod Commun. 10(7):1185-1190. (IF=0.906)
  • Lemmer Y, Kalombo L, Pietersen RD, Jones AT, Semete-Makokotlela B, Van Wyngaardt S, Ramalapa B, Stoltz AC, Baker B, Verschoor JA, Swai HS, de Chastellier C. (2015) Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. J Control Release. 211:94-104. (IF=7.705)
  • Uys P, Brand H, Warren RM, van der Spuy GD, Hoal EG, van Helden PD. (2015) The risk of tuberculosis reinfection soon after cure of a first disease episode is extremely high in a hyperendemic community. Plos One. 10(12):e0144487. (IF=3.234)
  • Du Plessis WJ, Walzl G, Loxton AG. (2015) B cells as multi-functional players during Mycobacterium tuberculosis infection and disease. Tuberculosis. (2015) 1-8. (IF=2.711)
  • Dawson R, Diacon AH, Everitt D, van Niekerk C, Donald PR, Burger DA, Schall R, Spigelman M, Conradie A, Eisenach K, Venter A, Ive P, Page-Shipp L, Variava E, Reither K, Ntinginya NE, Pym A, von Groote-Bidlingmaier F, Mendel CM. (2015) Efficiency and safety of the combination of moxifloxacin, pretomanid (PA-824), and pyrazinamide during the first 8 weeks of antituberculosis treatment: a phase 2b, open-label, partly randomised trial in patients with drug-susceptible or drug-resistant pulmonary tuberculosis. Lancet. 385(9979):1738-1747. (IF=45.217)
  • Boeree MJ, Diacon AH, Dawson R, Narunsky K, du Bois J, Venter A, Phillips PP, Gillespie SH, McHugh TD, Hoelscher M, Heinrich N, Rehal S, van Soolingen D, van Ingen J, Magis-Escurra C, Burger D, Plemper van Balen G, Aarnoutse RE; PanACEA Consortium. (2015) A dose-ranging trial to optimize the dose of rifampin in the treatment of tuberculosis. Am J Respir Crit Care Med. 191(9):1058-1065. (IF=12.996)
  • Heinrich N, Dawson R, du Bois J, Narunsky K, Horwith G, Phipps AJ, Nacy CA, Aarnoutse RE, Boeree MJ, Gillespie SH, Venter A, Henne S, Rachow A, Phillips PP, Hoelscher M, Diacon AH; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA); Pan African Consortium for the Evaluation of Antituberculosis Antibiotics PanACEA. (2015) Early phase evaluation of SQ109 alone and in combination with rifampicin in pulmonary TB patients. J Antimicrob Chemother. 70(5):1558-1566. (IF=5.313)
  • Diacon AH, Dawson R, von Groote-Bidlingmaier F, Symons G, Venter A, Donald PR, van Niekerk C, Everitt D, Hutchings J, Burger DA, Schall R, Mendel CM. (2015) Bactericidal activity of pyrazinamide and clofazimine alone and in combinations with pretomanid and bedaquiline. Am J Respir Crit Care Med. 191(8):943-953. (IF=12.996)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total:161)

Plenary/Keynote Lectures (11)

  • Walzl G. Challenges facing preclinical TB vaccine development. Aeras TB vaccine symposium, 46th Union World Conference on Lung Health, Cape Town, South Africa, 2-6 December 2015.
  • Walzl G, Ronacher K, Malherbe S, Chegou NN, Stanley K, van der Spuy GD, Kriel M, Kidd M, Warwick J, Barry CE 3rd, Chen R, Dodd L, Johnson JL, Boom WH, Peppard T, Cliff J, Dockrell H, Schoolnik G, Dolganov G, Alland D, Zak D, Winter J. Potential and limitations of current candidate host biomarkers for treatment response. Symposium: Spelunking for Treasure: Searching for Candidate Biomarkers as Prognostic Indicators for Treatment Outcome. 46th Union World Conference on Lung Health, Cape Town, South Africa, 2-6 December 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Plenary lecture, HHMI Science Meeting, Janelia Farm Research Campus, Ashburn, MD, 9-11 June 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Invited lecture, K-RITH, Durban, 26 June 2015.
  • Mizrahi V. MM4TB Project Update from Cape Town. MM4TB Consortium Meeting #10, Institut Pasteur, Paris, 29 June – 1 July 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Plenary lecture, Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015.
  • Evans J, Trujillo C, Ehrt S, Boshoff HIM, Schnappinger D, Mizrahi V. A pathway approach to identifying vulnerable and bactericidal targets in Coenzyme A biosynthesis in M. tuberculosis. Plenary lecture, Gordon Research Seminar on Tuberculosis Drug Discovery & Development, Girona, Spain, July 11-12 2015.
  • Mizrahi V. Identifying vulnerable steps in the CoA pathway of M. tuberculosis. Plenary Lecture, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015.
  • Mizrahi V. Undertaking the full spectrum of research in a TB-endemic country: the example from South Africa. Plenary lecture, 46th Union World Conference on Lung Health, Cape Town, 5 December 2015.
  • Van Helden PD. Drug resistance and the contribution of zoonotic TB Plenary lecture, 46th Union World Conference on Lung Health, Cape Town, 2-6 December 2015.
  • Van Helden PD. Bovine TB in wildlife. Plenary Lecture, Workshop on Accelerating bTB Control in Developing Countries, Rabat, Morocco, 2-6 December 2015.

Invited Talks (81)

  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 9-11 June 2015. Annual meeting of the Howard Hughes Medical Institute. Janelia Farm Research Campus, Ashburn, Virginia, USA
  • Kana BD. Detection, quantification and characterization of differentially culturable tubercle bacteria in human TB disease. TB Biomarkers Annual Meeting. 3 – 5 June 2015, the Bill and Melinda Gates Foundation, Seattle, USA.
  • Kana BD. Differential bacterial growth states in active TB disease. “The New Post-2015 Global TB Strategy? The End Game”. 24 March 2015, The National Institute for Communicable Diseases, Sandringham, Johannesburg.
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 26 November 2015, The Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 1 October 2015, The Perinatal HIV Research Unit, Soweto, Johannesburg, South Africa
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease: Separation anxiety and schizophrenia in mycobacterial cells. 16 January 2015, The Kwazulu-Natal Institute for TB and HIV, Durban, South Africa.
  • Chegou N. Accuracy of a Seven-marker Host Serum Protein Biosignature in the Diagnosis of TB Disease in African Primary Health Care Clinic Attendees Presumed to have TB. 46th Union World Conference on Lung Health, Cape Town, 2 - 6 December 2015.
  • Chengalroyen M, Beukes G, Gordhan B, Neil Martinson, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral presentation, 15 – 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa
  • Chengalroyen M, Beukes B, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral Presentation, 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral Presentation, October 2015, Postdoctoral Symposium, Wits University, Johannesburg, South Africa.
  • Gordhan B. The contribution of Nth and Nei DNA glycosylases to mutagenesis in Mycobacterium smegmatis. Oral Presentation, 15 – 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable and non-culturable bacteria in patients receiving first-line TB treatment, Oral presentation, 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Peters J, McIvor A, Papadopoulous A, Gordhan B, Kana B. Assessing the utility of Fatty acids in resuscitating DCTB from the sputum of TB infected patients. Oral and Poster. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • Peters J, McIvor A, Papadopoulous A, Gordhan B, Kana B. Establishing patterns in drug treatment response rates of tuberculosis disease based on non-culturable Mycobacterium tuberculosis in sputum. Oral presentation. 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable versus non-culturable bacteria in patients receiving first-line TB treatment: An endpoint analysis, Oral presentation, 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • Ealand C, Kana B. DacB: An essential enzyme for mycobacterial growth, Poster with short talk, 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa
  • Ealand C, Kana B. An essential DD-carboxypeptidase determines localisation of peptidoglycan synthesis in mycobacteria, Oral, Health Sciences – Symposium for Postdoctoral and Carnegie Fellows, Johannesburg, South Africa.
  • Mizrahi V. Global health networks and networking. Invited lecture, Imperial-UCT Graduate Summer School, Global Health Fellows Programme, University of Cape Town, 26 January 2015.
  • Mizrahi V. The state of TB drug development and the role civil society can play. Treatment Action Campaign & Section 27 Symposium on Tuberculosis, Cape Town, 23 June 2015
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Talk presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Talk presented at the Pathology research day. FMHS, Stellenbosch University, Cape Town, South Africa, 04 June 2015.
  • Hoal EG. Genomic Disease Associations in TB in non-Euro-American Populations: The impact of ancestry. Talk presented at the NIAID Workshop on Host Response to TB-HIV Infection: A Genomic Perspective. NIAID, Rockville, USA, 14 January 2015.
  • Walzl G. Host biomarkers for active TB and for treatment response. South African Thoracic Society Conference, Cape Town, South Africa, 7-10 August 2015.
  • Walzl G. Deconfounding latent tuberculosis. South African Thoracic Society Conference, Cape Town, South Africa, 7-10 August 2015.
  • Walzl G. Host biomarkers for active TB and for treatment response. ACTG Annual Meeting, Washington DC, USA, June 2015
  • Walzl G. Immunodiagnostics of active TB disease. 4th Global Forum on TB Vaccines, Stop TB Partnership Working Group on New Vaccines. Shanghai, China, 21-24 April 2015.
  • Walzl G, Malherbe S, Ronacher K, Thiart L, Stanley K, van der Spuy GD, Kriel M, Warwick J, Barry CE 3rd, Peppard T, Alland D, Gregory Dolganov, Gary Schoolnik, Winter J. Human TB Treatment Response Studies Using PET/CT Imaging: Inconvenient Observations. Host Response in Tuberculosis- Keystone Symposia. Santa Fe, New Mexico, USA. January 22-27, 2015.
  • Warren RM. Genesis of drug resistance in Tuberculosis in South Africa. Plenary Talk presented at the PASER, HIVDR in the upcoming fight against antimicrobial resistance. Rainbow Towers Hotel, Harare, Zimbabwe, 08 May 2015.
  • Warren RM. The eveolving global TB epidemic- Focus on South Africa. Talk presented at the 5th Tuberculosis Control symposium. Education centre, The Woolcock, Glebe, Australia, 03-04 September 2015.
  • Klopper M. 1.Drug-resistance beyond XDR-TB: a health situation beyond repair? Talk presented at the Ukwanda Sustainable Rural Health Research Days. Worcester Campus, Stellenbosch University, Worcester, South Africa, 18-19 March 2015
  • Warren RM. Transmission of drug resistant TB- lessons from South Africa. Talk presented at the 5th Conference of the Union Asia Pacific Region. The Hilton Hotel, Sydney, Australia, 31 August - 02 September 2015.
  • Smith B. Application of Becton Dickinson FACSTM Combinatorial Antibody Profile (FACSTM CAP) technology to the identification of efficiency of tuberculosis therapy. Talk presented at the Stellenbosch University Annual Academic Year day. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Moller M. Population admixture: friend or foe in TB susceptibility. Talk presented at the 6th Biennial Congress of the Southern African Society of Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16 August - 19 September 2015.
  • le Roex N. Disease Control in Wildlife: BTB Test & Cull in African Buffalo. Talk presented at the International Congress of Conservation Biology (ICCB) 2015. Le Corum, Montpellier, France, 02-06 August 2015.
  • Moller M. Genome-wide association study of ancestry-specific TB risk in the South African Coloured population. Talk presented at the African Symposium on Genome Wide Association Studies for complex disease. African Institute for Mathematical Sciences of SA, Cape Town, South Africa, 23-24 April 2015.
  • Daya M. Using multi-way admixture mapping to elucidate TB susceptibility in the South African Coloured population. Talk presented at the African Symposium on Genome Wide Association Studies for complex disease. African Institute for Mathematical Sciences of SA, Cape Town, South Africa, 23-24 April 2015.
  • Klopper M. Next generation sequencing reveals hidden ethionamide resistance as a likely driver towards resistance beyond XDR-TB in a high burden population. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Tabb DL. Lipid Identification with Greazy and LipidLama. Talk presented at the Special Seminar for Department of Microbiology, Immunology, and Pathology. Colorado State University, Ft. Collins, CO, United States, 10 November 2015.
  • Whitfield M. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Whitfield M. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Kreiswirth B. Virtual sequencing of the entire pncA gene target in a single tube using LATE-PCR and Lights-On/Lights-Off probes to predict PZA susceptibility. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Kinnear C. Mutation Screening for Primary immunodeficiency disorders in a tuberculosis endemic region: a South African perspective. Talk presented at the 16th Biennial Congress of the South African Society for Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16-19 August 2015.
  • Kinnear C. Primary Immunodeficiency Disease management in tuberculosis endemic regions - are we aware -and how does a registry assist? Talk presented at the 4th African Society for Immunodeficiencies Congress. Hotel el Aurassi, Algiers, Algeria, 29-31 May 2015.
  • Sampson SL. Devastating Diseases and a Sustainable Society: Learning from the Tubercle Bacillus. Talk presented at the Lustrum conference, Science for Sustainability. Vrije University, Amsterdam, The Netherlands, 26 December 2015.
  • Sampson SL. Royal Society/Newton Fund International Exchanges Scheme. Talk presented at the Lustrum conference, Science for Sustainability. Vrije University, Amsterdam, The Netherlands, 26 November 2015.
  • Sampson SL. Single cell elucidation of mycobacterial replication dynamics. Talk presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Sampson SL. Single cell elucidation of mycobacterial replication dynamics. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Chegou NN. Host Serum Biosignatures for the diagnosis of TB disease. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 2-6 December 2015.
  • Willemse D. Rv1460 is required for growth of Mycobacterium tuberculosis in vitro. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Roos EO. Detecting Mycobacterial Infections in African Warthogs (Phacochoerus africanus) Using Serological Methods. Talk presented at the 6th Annual Wildlife Research Symposium. Onderstepoort, Pretoria, South Africa, 19-20 November 2015.
  • Theron G. Meet the Expert: Opportunities and challenges in TB diagnostic research: Overview and lessons learnt from two large diagnostic RCTs (Xpert and LAM). Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 06 December 2015.
  • Theron G. Point of care diagnostics for tuberculosis: where are we, and what is next?. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04 December 2015.
  • Theron G. Impact of Xpert MTB/RIF on TB case detection and treatment: experience in high-HIV settings. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Peter J, Theron G. Rapid point-of-care urine-based testing for tuberculosis and its impact on mortality: a multi-centre randomised controlled trial. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Lange B, Theron G. Diagnostic accuracy of the Xpert® MTB/RIF cycle threshold level to predict smear positivity in respiratory samples: a systematic review and meta-analysis. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Khaki A, Theron G. The effect of automated nucleic acid amplification assays on mortality in routine care settings: meta-analysis of individual participant data. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Loxton AG. TB immunology Research at Stellenbosch University Immunology Research Group. Annual ACTG Network Meeting, Washington, DC, 22 – 26 June 2015.
  • Pule C. Deciphering the physiology of drug tolerant and resistant Mycobacterium tuberculosis. Talk presented at the The 9th Annual Medical Research Council Early Career Scientist Conference. SAMRC conference centre, Cape Town, South Africa, 18-20 October 2015.
  • De Vos M. Rapid Single tube diagnosis of M(X)DR-TB. Talk presented at the Hain LifeSciences TB symposium. Holiday Inn, Harare, Zimbabwe, 25-26 March 2015.
  • De Vos M. The cutting edge of drug resistance diagnostics. Talk presented at the 6th FIDSSA congress. Champagne Sports Resort, Winterton, South Africa, 05-08 November 2015
  • De Vos M. Heterogeneity as revealed by whole genome sequencing in Mycobacterium tuberculosis. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • De Vos M. Construction and validation of a three colour single-tube assay for the detection of resistance to first- and second-line anti-TB antibiotics. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Ngwane A. Translational research in tuberculosis drug development. Talk presented at the Postdoctoral Research Symposium. Stellenbosch University, Cape Town, South africa, 29-30 September 2015.
  • Malherbe S. Evaluating TB treatment responses by [18F]FDG-PET/CT imaging – during and after treatment. 5th TB Biomarker Annual Meeting, Seattle, WA, 4 June 2015.
  • Miller MA. Elephant medicine and husbandry. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS. Continental Hotel, Canela, Brazil, 06 October 2015.
  • Miller MA. Tuberculosis in South African wildlife - why is it important? Talk presented at the Stellenbosch University Inaugural Lecture. FMHS, Stellenbosch University, Cape Town, South Africa, 23 June 2015.
  • Miller MA. Elephant medicine and husbandry. Invited Talk presented at the NIH Many Hosts of Mycobacteria Symposium. TNPRC, Covington, USA, 26 March 2015.
  • Miller MA. Why is diagnosis of TB so difficult? Talk presented at the BTB Outreach Day. Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa, 02 March 2015.
  • Miller MA. Tuberculosis diagnostic challenges in wildife and public health implications. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 05 December 2015.
  • Miller MA. Novel approaches to detection of tuberculosis in African wildlife. Talk presented at the US Animal Health Association annual conference. Providence Convention Center, Providence, USA, 26 October 2015.
  • Miller MA. Serodiagnostics for TB in wildlife species - can these play a role in detecting disease? Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Elephant TB - overview of clinical aspects, diagnosis, management and zoonotic risk. Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Warthog BTB - are they an underrecognized threat for BTB control?. Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Rumenitis and feed management in exotic ruminants. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Restraint, sedation, and immobilization of captive elephants and rhinos. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Hippopotamus medicine and immobilization. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Field research in megavertebrate immobilization. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 05 October 2015.
  • Miller MA. Translating techniques used with free-ranging megavertebrates to zoo medicine. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015
  • Miller MA. Tuberculosis in wildlife in South Africa - are we winning the battle?. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015.
  • Miller MA. Diagnosis, management, and control of tuberculosis in captive elephants and rhinos. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015.
  • Posters (69)
  • Senzani S, Bhaskar A, li D, Betzig E, Dhar N, Kana B. Identification and Characterization of Mycobacterial cell wall amidases. Oral and Poster. Swiss South Africa Joint Research Programme, Basel Switzerland.
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, School of Clinical Medicine Research Day. September 2015, Wits University, Johannesburg, South Africa
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Moseki MR, Kana B. Functional analysis of cydDC-encoded type ABC transporter in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Nthambeleni GS, Kana B, Gordhan B. Is there a combined role for the Nth and MutY DNA glycosylases during DNA repair in Mycobacterium smegmatis? 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Hassim F, Papadopoulos AO, Kana BD, Gordhan BD. A combinatorial role for DNA glycosylases in mutation avoidance in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Papadopoulos A, Kana B. In silico analysis of M23-domain activators of peptidoglycan degrading amidases in Mycobacterium tuberculosis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Narrandes N, Kana B. Characterization of the electron transport chain in mycobacteria, 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z,Kana B. Detection of differentially culturable tubercle bacilli by exogenous cyclic-AMP in drug-susceptible patients at baseline,  15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable and non-culturable TB bacteria in patients receiving first-line treatment, 9th Annual SAMRC Early Career Scientist Convention
  • Shaku M, Kana B. Characterization of LytM domain-containing proteins in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa
  • Maphatsoe MM, Ealana C, Kana B. Localization of low molecular weight proteins and other potential cell wall remodeling enzymes. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Rantsi TC, Kana B, Gordhan B. Molecular basis of the interplay between the Nth and the Nei DNA glycosylases in the base excision repair pathway in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Sheik Ismail Z, Ealand C, Kana B. Characterization of mycobacterial DD- carboxypeptidases. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa. WON FIRST PRIZE
  • Sheik Ismail Z, Ealand C, Kana B. Characterization of mycobacterial DD- carboxypeptidases. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Ralefeta D, Machowski E, Kana B. Heterologous expression and characterisation of Mycobacterium tuberculosis DD-Carboxypeptidases in Mycobacterium smegmatis. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • Ralefeta D, Machowski E, Kana B. Mycobacterial DD-Carboxypeptidases: Filling In The Gaps. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Ealand C, Kana B. An essential DD-carboxypeptidase determines localization of peptidoglycan synthesis in mycobacteria, Poster, 9th Annual SAMRC Early Career Scientist Convention.
  • Naran K. Construction and characterization of bioluminescent Mycobacterium tuberculosis reporter strains for mechanism of action studies in new TB drug discovery. Invited talk, K-RITH/IDM Scientific Symposium, Zimbali Lodge, Durban, 22-24 November 2015
  • Singh V. Identification and validation of GuaB2 as a tuberculosis drug target.  Invited talk, K-RITH/IDM Scientific Symposium, Zimbali Lodge, Durban, 22-24 November 2015
  • Evans J, Trujillo C, Ehrt S, Boshoff HIM, Schnappinger D, Mizrahi V. A pathway approach to identifying vulnerable and bactericidal targets in Coenzyme A biosynthesis in M. tuberculosis. Gordon Research Seminar on Tuberculosis Drug Discovery & Development, Girona, Spain, July 11-12 2015
  • Singh V, Pato J, Haartkorn R, Warner DF, Rizzi M, Keri G, Mizrahi V. Identification and validation of GuaB2 as a drug target. Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015
  • Mukherjee R, Ioerger T, Warner DF, Mizrahi V. Permeation in M. tuberculosis. Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015
  • Koch A, Brites D, Evans JC, Seldon R, Oni T, Nicol MP, Warner DF, Mizrahi V, Harris D, Parkhill J, Gagneux S, Martin DP, Wilkinson RJ. High Resolution Snapshot of Genetic Diversity within Mycobacterium tuberculosis in a Region of High HIV Co-Infection. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Mukherjee R, Ioerger T, Warner DF, Mizrahi V. Small hydrophilic molecule permeation in M. tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Broadley S, Warner DF, Sewell T. Structure determination of proteins involved in induced mutagenesis in Mycobacterium tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Singh V, Kigondu EM, Chibale K, Mizrahi V, Warner DF. Chlorpromazine potentiates the activity of spectinomycin against Mycobacterium tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015.
  • Bunjun R, Rious C, Soares A, Hanekom W, Walzl G, Wilkinson R, Burgers W. Defects in multiple mycobacterial T helper subsets in blood and lungs in early HIV infection. Host Response in Tuberculosis- Keystone Symposia. Santa Fe, New Mexico, USA. 22-27 January 2015.
  • Corstjens PLAM, Tjon Kon Fat EM, Chegou NN, Ottenhoff THM, Walzl G, Geluk A. Assessment of lateral flow based assay in six African countries to determine IP-10 levels in stimulated whole blood samples of TB suspects. Host Response in Tuberculosis - Keystone Symposia. Santa Fe, New Mexico, USA. 22-27 January 2015.
  • Hammond-Aryee K. A high seroprevalence of Toxoplasma gondii antibodies in a population of feral cats in the Western Cape of South Africa. Poster presented at the13th International congress on Toxoplasmosis and Toxoplasma gondii research. Gettysberg College, Pennsylvania, USA, 17-20 June 2015.
  • Hammond-Aryee K. The prevalence of antibodies to Toxoplasma gondii in sheep in the Western Cape of South Africa. Poster presented at the13th International congress on Toxoplasmosis and Toxoplasma gondii research. Gettysberg College, Pennsylvania, USA, 17-20 June 2015.
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Poster presented at the59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Dippenaar A. Investigating microevolution in M. tuberculosis during transmission. Poster presented at the 36th annual Congress of the European Society of Mycobacteriology. Bellevue Park Hotel Riga, Riga, Latvia, 28 June - 01 July 2015.
  • Miller M, Parsons S, Buss P, Lyashchenko K, van Helden PD. Detection of immunological responses to BTB in African wildlife. Poster presented at the Keystone Symposium on Immunity to Veterinary Pathogens:  Informing Vaccine Development. Keystone resort, Colorado, USA, 20-25 January 2015.
  • Chegou, N. Diagnostic performance of a seven-marker serum protein biosignature for the diagnosis of active tb disease in African primary health care clinic attendees with suspected pulmonary tuberculosis. The Merck Africa Research Summit, in Geneva, Switzerland, from the 19-20 October 2015.
  • Da Camara NL. Large pncA deletions in Mycobacterium tuberculosis influence rapid detection of pyrazinamide susceptibility. Poster presented at the 5th Conference of The Union Asia Pacific Region. Hilton Hotel, Sydney, Australia, 31 August - 02 September 2015.
  • Da Camara NL. Targeted deep sequencing to detect heterogeneity in Mycobacterium tuberculosis populations. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kleynhans L, Ruzive S, Van der Spay G, Van Helden, PD, Walzl G, Ronacher, K. Evaluation of the endocrine changes during TB treatment. 4th European Congress of Immunology. Vienna, Austria, 6-9 September 2015.
  • Sao Emani C, Williams MJ, Wiid IJ, Baker B. Investigation of role of ergothioneine in Mycobacterium tuberculosis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Da Camara NL. Targeted deep sequencing of drug resistance associated genes to investigate heterogeneity in Mycobacterium tuberculosis populations. Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Parbhoo T, Mouton M, Sampson S. Optimization of Flow Cytometric Methods for Mycobacterial Viability Discrimination and Cell Enumeration. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kriel N, Heunis T, Williams M, Sampson S, Warren RM, van Helden PD. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Kriel N, Heunis T, Williams M, Sampson S, Warren RM, van Helden PD. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kriel N, Heunis T, Sampson S, van Helden PD, Warren RM, Williams M. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Salie M, Daya M, Lucas LA, Warren RM, van der Spuy GD, van Helden PD, Hoal EG, Moller M. An investigation of toll-like receptors in tuberculosis susceptibility reveals sex-specific associations for TLR8 polymorphisms. Poster presented at the American Society of Human Genetics 2015 Annual Meeting. Baltimore Convention Centre, Baltimore, USA, 06-10 October 2015.
  • Kunsevi-Kilola C, Parbhoo T, Tshivhula H. Identification of distinct bio-signatures in whole blood and live M.tb stimulated PBMCs in TB house hold contacts with and without type 2 diabetes. Poster presented at the 1st IUIS-FAIS Southern African Immunology workshop and 6th Infectious Diseases in Africa Symposium. River Club, Cape Town, South Africa, 20-24 October 2015.
  • Salie M, Hoal EG. Investigation of TLR in TB susceptibility reveals sex-specific associations for TLR8. Poster presented at the ASHG conference. Baltimore conference centre, Baltimore, USA, 08 October 2015.
  • Daya M, Hoal EG. Using multi-way admixture mapping to elucidate TB susceptibility in the South african Coloured population. Poster presented at the ASHG conference. Baltimore conference centre, Baltimore, USA, 07 October 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Poster presented at the Southern African Society for Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16-19 August 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the Postdoctoral Research Day. Stellenbosch University, Cape Town, South Africa, 29 October 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the 1st Biomedical Sciences Annual Research Day. FISAN Building, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Dippenaar A. Whole genome sequence analysis of Mycobacterium suricattae. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Whitfield M, Streicher EM, Mardarowicsz I, Scott L, Stevens W, Sampson SL,  van Helden PD, Warren RM, van Rie A. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Van Rie A, Whitfield M, Scott L, Warren RM, Voss De Lima Y, Stevens W, Sirgel F. Potential of Rifabutin in the Treatment of Rifampicin Resistant Tuberculosis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Visser H, de Vos M, van der Merwe RG, van Helden PD, Warren RM, Victor TC, Paul LV. Clofazimine: Mechanism of Resistance within M. tuberculosis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04 December 2015.
  • Sampson S, Mouton JM, Helaine S, Holden DW. Single cell elucidation of mycobacterial replication dynamics .Poster presented at the New Approaches and Concepts in Microbiology. EMBL, Heidelberg, Germany, 11-14 October 2015.
  • Chegou NN. Host biomarkers for the diangosis of TB disease. Poster presented at the UNESCO-Merck Africa Research Summit. Maotel Royal Hotel and Eden Palace Au Lac, Geneva, Switzerland, 19-20 October 2015.
  • Willemse D, Warren RM, Williams MJ. Generation and phenotypic characterisation of Rv1460 mutants of Mycobacterium tuberculosis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Willemse D, Weber B, Warren RM, Williams MJ. Expression and purification of the Mycobacterium tuberculosis Rv1460, a possible suf system regulator. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Clarke C. Development of a Diagnostic Assay for Mycobacterium suricattae Infection in Meerkats (Suricata suricatta). Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Roos EO. Diagnostic Tool Evaluation: Detection of Mycobacterial Infections in Warthogs (Phacochoerus africanus) Using Serological Tests. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Theron G. Psychological distress and its relationship with nonadherence to TB treatment: a multicentre study. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 03 December 2015.
  • Calligaro G, Theron G, Khalfey H, Peter J, Miller M, Michell L, Joubert I, Dheda K. randomised controlled trial of the impact of Xpert® on tracheal aspirates within a burden of disease study in South African intensive care units. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Limberis J, Pietersen E, Jayakumar JJ, Theron G, Dheda K, Smith L, Warren RM, Clark T. Genetic markers of drug resistance and their association with clinical outcomes in patients with MDR-TB or XDR-TB. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • De Vos M. Whole genome sequencing reveals genetic heterogeneity and suggests the role of selective bottleneck in defining the population structure of Mycobacterium tuberculosis clinical isolates. Poster presented at the 46th Union World Conferences on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Ngwane A, Baker B, Wiid I, van Helden PD. Design, synthesis of in vitro antitubercolosis activity of 2(5H) furanones. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 05 August 2015.
  • Kunsevi-Kilola C, Tshivula H. Characterization of human alveolar macrophage and blood monocyte derived macrophage responses to Mycobacterium tuberculosis in TB household contacts with and without type 2 diabetes. Poster presented at the 6th IDA Symposium & African Flow Cytometry Workshop. The River Club, Cape town, South Africa, 20-24 October 2015.

Products / Artifacts / Patents (2)

  • Method for diagnosing tuberculous meningitis.  Inventors: Chegou, NN, Walzl, G, van Furth, AM, Visser, DH, Applicant: Stellenbosch University. South African Provisional patent Application No: 2014/02743, Filing date: 2014/04/15. Worldwide (PCT) application was filed on the 15/04/2015 with application number: PCT/IB2015/052751, Status: Pending
  • Method for diagnosing tuberculosis. Inventors: Chegou, NN, Walzl, G, Mihret, A, Applicant: Stellenbosch University, Application type: PCT, claiming priority from the South African provisional patent: ZA 2014/01456, Country: PCT / WIPO Application No: PCT/IB2015/051435, Filing date: 2015/02/26, Status: Pending

 

 


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