Research

The research conducted in the laboratories of the CBTBR is internationally recognized. The two nodes of the CBTBR are positioned adjacently on the research continuum. The University of the Witwatersrand (Wits) node of the CBTBR is at the forefront of fundamental research in mycobacterial metabolism that is aimed at identifying, validating and characterizing novel drug targets and vaccine candidates for TB. The major areas of interest in this laboratory include the molecular mechanisms underlying the regulation of dormancy and persistence in M. tuberculosis and the mechanisms of DNA metabolism underlying the development of strain diversification and the evolution of drug resistance. These areas are being investigated using a highly inter-disciplinary approach involving molecular genetics, biochemistry, microbial physiology, bioinformatics, comparative genomics and transcriptomics.

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The Stellenbosch University (SU) node currently occupies a position bridging the gap between basic research and its application in clinical TB research and management. This laboratory is engaged in fundamental research towards the identification and characterisation of novel drug targets and towards a better understanding of the biology of the bacterium, which enables it to avoid destruction in the host and spread rapidly within human populations. It is also at the cutting edge of research to identify novel bacterial and host markers that will considerably shorten the time taken to evaluate new drug and vaccines and to develop new diagnostic tools and new, multidisciplinary approaches for understanding the epidemiology of the disease. Each one of these efforts is aimed at bridging the gap between fundamental research and its clinical application and some have broader application beyond the field of human TB, most notably in areas such as wildlife management and veterinary disease. For these reasons, the SU node has developed an extensive, collaborative clinical research network, which will be essential for the successful application of post-genomic research findings in a clinical setting.

The research focus of the CBTBR thus falls directly within the theme of 'cutting edge technology development for diseases of poverty' identified in the National R&D Strategy of the Department of Science and Technology. The highly integrated, inter- and multidisciplinary approach that is being adopted provides a particularly powerful platform for the training of young scientists on the new-to-established researcher track in state-of-the-art health research. The vast network of collaborations with which the laboratories are involved will also provide considerable international training opportunities for researchers associated with the CBTBR in leading institutions around the world.

Publications and Conferences

2015

* The Names in bold are CBTBR staff and students
Books / Chapters in Books (Total: 1)

  • Walzl G, Haks MC, Joosten SA, Kleynhans L, Ronacher K, Ottenhoff THM. Clinical immunology and multiplex biomarkers of human tuberculosis. In: Tuberculosis. Editors Kaufmann SHE, Rubin EJ, Zumla A. Cold Spring Harbour Laboratory Press, New York, 2015. P 197-209.

Articles in Peer-Reviewed Journals (Total: 84)

  • Hassim F, Papadopoulos AO, Kana BD, Gordhan BG. (2015) A combinatorial role for MutY and Fpg DNA glycosylases in mutation avoidance in Mycobacterium smegmatis. Mutat Res. 779: 24-32. (IF=3.680)
  • Narrandes NC, Machowski EE, Mizrahi V, Kana BD. (2015) Cleavage of the moaX-encoded fused molybdopterin synthase from Mycobacterium tuberculosis is necessary for activity. BMC Microbiol. 15:22. (IF=2.729)
  • Williams MJ, Shanley CA, Zilavy A, Peixoto B, Manca C, Kaplan G, Orme IM, Mizrahi V, Kana BD. (2015) bis-Molybdopterin guanine dinucleotide is required for persistence of Mycobacterium tuberculosis in guinea pigs. Infect Immun. 83: 544-550. (IF=3.731)
  • Lebina L, Abraham PM, Motlhaoleng K, Rakgokong M, Variava E, Martinson NA. (2015) The use of decentralized GeneXpert by trained non-laboratory technicians in rural clinics in South Africa. Tuberculosis (Edinb). 95: 625-626. (IF=2.711)
  • Lala SG, Little KM, Tshabangu N, Moore DP, Msandiwa R, van der Watt M, Chaisson RE, Martinson NA. (2015) Integrated Source Case Investigation for Tuberculosis (TB) and HIV in the Caregivers and Household Contacts of Hospitalised Young Children Diagnosed with TB in South Africa: An Observational Study. PLoS One 10:e0137518. (IF=3.234)
  • Lebina L, Abraham PM, Milovanovic M, Motlhaoleng K, Chaisson RE, Rakgokong M, Golub J, Variava E, Martinson NA. (2015) Latent tuberculous infection in schoolchildren and contact tracing in Matlosana, North West Province, South Africa. Int J Tuberc Lung Dis. 19:1290-1292. (IF=2.315)
  • Omar T, Variava E, Moroe E, Billioux A, Chaisson RE, Lebina L, Martinson NA. (2015) Undiagnosed TB in adults dying at home from natural causes in a high TB burden setting: a post-mortem study. Int J Tuberc Lung Dis. 19:1320-1325. (IF=2.315)
  • Singh V, Brecik M, Mukherjee R, Evans JC, Svetlíková S, Blaško J, Blackburn J, Warner DF, Mikušová K, Mizrahi V. (2015) The complex mechanism of antimycobacterial action of 5-fluorouracil. Chem. Biol. 22:63-75. (IF=6.645)
  • Gopinath K, Warner DF, Mizrahi V. (2015) Targeted gene knockout and essentiality testing by homologous recombination. Methods Mol Biol. 1285:131-149. (IF=1.290)
  • Evans JC, Mizrahi V. (2015) The application of tetracycline regulated gene expression systems in the validation of novel drug targets in Mycobacterium tuberculosis. Front Microbiol. 6:812. (IF=3.989)
  • van der Westhuyzen R, Winks S, Wilson CR, Boyle GA, Gessner RK, Soares de Melo C, Taylor D, de Kock C, Njoroge M, Brunschwig C, Lawrence N, Rao SP, Sirgel F, van Helden PD, Seldon R, Moosa A, Warner DF, Arista L, Manjunatha UH, Smith PW, Street LJ, Chibale K. (2015) Pyrrolo[3,4-c]pyridine-1,3(2H)-diones: A Novel Antimycobacterial Class Targeting Mycobacterial Respiration. J. Med. Chem. 58(23): 9371-9381. (IF=5.447)
  • Soares de Melo C, Feng TS, van der Westhuyzen R, Gessner RK, Street LJ, Morgans GL, Warner DF, Moosa A, Naran K, Lawrence N, Boshoff HI, Barry CE 3rd, Harris CJ, Gordon R, Chibale K. (2015) Aminopyrazolo [1,5-a]pyrimidines as potential inhibitors of Mycobacterium tuberculosis: Structure activity relationships and ADME characterization. Bioorg Med Chem. 23(22):7240-7250. (IF=2.420)
  • Warner DF, Koch A, Mizrahi V. (2015) Diversity and disease pathogenesis in Mycobacterium tuberculosis. Trends Microbiol. 23(1):14-21. (IF=9.186)
  • Jones CE, Hesseling AC, Tena-Coki NG, Scriba TJ, Chegou NN, Kidd M, Wilkinson RJ, Kampmann B. (2015) The impact of HIV exposure and maternal Mycobacterium tuberculosis infection on infant immune responses to Bacille Calmette-Guérin vaccination. AIDS. 29(2):155-165. (IF=5.554)
  • Chigutsa E, Pasipanodya JG, Visser ME, van Helden PD, Smith PJ, Sirgel FA, Gumbo T, McIlleron H. (2015) The impact of non-linear interactions of pharmacokinetics and MICs on sputum bacillary kill rates as a marker of sterilizing effect in tuberculosis. AAC. 59(1):38-45. (IF=4.476)
  • Visser DH, Solomons RS, Ronacher K, van Well GT, Heymans MW, Walzl G, Chegou NN, Schoeman JF, van Furth AM. (2015) The host immune response to tuberculous meningitis. Clinical Infectious Diseases. 60(2):177-187. (IF=8.886)
  • Fang Z, van der Merwe RG, Warren RM, Schubert W-D, Gey van Pittius NC. (2015) Assessing the progress of Mycobacterium tuberculosis H37Rv structural genomics. Tuberculosis. 95(2):131–136. (IF=2.711)
  • Fang Z, Sampson SL, Warren RM, Gey van Pittius NC, Newton-Foot M. (2015) Iron acquisition strategies in mycobacteria. Tuberculosis. 95(2):123–130. (IF=2.711)
  • Ronacher K, Joosten S, van Crevel R, Dockrell H, Walzl G, Ottenhoff T. (2015) Acquired immunodeficiencies and tuberculosis: Focus on HIV/AIDS and Diabetes Mellitus. Immunological Reviews. 264(1):121-137. (IF=10.120)
  • Mbugi E, Katale B, Siame K, Keyyu J, Kendall S, Dockrell H, Streicher E, Michel A, Rweyemamu M, Warren R, Matee M, van Helden P. (2015) Genetic diversity of Mycobacterium tuberculosis isolated from tuberculosis patients in the Serengeti ecosystem in Tanzania. Tuberculosis. 95(2):170-178. (IF=2.711)
  • Miller M, Buss P, Hofmeyr J, Olea-Popelka F, Parsons S, van Helden P. (2015) Antemortem Diagnosis of Mycobacterium bovis Infection in Free-Ranging African Lions (Panthera leo) and Implications for Transmission. Journal of Wildlife Disease. 51(2): 493-497. (IF=1.355)
  • Le Roex N, Cooper D, van Helden PD, Hoal EG, Jolles AE. (2015) Disease Control in Wildlife: Evaluating a Test and Cull Programme for Bovine Tuberculosis in African Buffalo. Transbound Emerg Dis. (Epub Jan 2015). (IF=2.944)
  • Merker M, Blin C, Mona S, Duforet-Frebourg N, Lecher S, Willery E, Blum M, Rüsch-Gerdes S, Mokrousov I, Aleksic E, Allix-Béguec C, Antierens A, Augustynowicz-Kopeć E, Ballif M, Barletta F, Beck HP, Barry CE 3rd, Bonnet M, Borroni E, Campos-Herrero I, Cirillo D, Cox H, Crowe S, Crudu V, Diel R, Drobniewski F, Fauville-Dufaux M, Gagneux S, Ghebremichael S, Hanekom M, Hoffner S, Jiao WW, Kalon S, Kohl TA, Kontsevaya I, Lillebæk T, Maeda S, Nikolayevskyy V, Rasmussen M, Rastogi N, Samper S, Sanchez-Padilla E, Savic B, Shamputa IC, Shen A, Sng LH, Stakenas P, Toit K, Varaine F, Vukovic D, Wahl C, Warren RM, Supply P, Niemann S, Wirth T. (2015) Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage. Nature Genet. 47(3):242-249. (IF=29.352)
  • Mikota SK, Gairhe K, Giri K, Hamilton K, Miller M, Paudel S, Lyashchenko K, Larsen RS, Payer JB, Waters R, Greenwald R, Dumonceaux G, Vincent B, Kaufman GE. (2015) Tuberculosis surveillance of elephants (Elephas maximus) in Nepal at the captive-wild interface. European Journal of Wildlife Research. 61(2): 221-229. (IF=1.634)
  • Dippenaar A, Warren RM. (2015) Tuberculosis: Fighting an old disease with next-generation sequencing. eLife. 4:e06782. (IF=9.322)
  • Miller MA, Greenwald R, Lyashchenko KP. (2015) Serodiagnosis of TB in black rhinoceros. Journal of Zoo and Wildlife Medicine. 46(1): 100–104. (IF=0.424)
  • Locher CP, Jones SM, Hanzelka BL, Perola E, Shoen CM, Cynamon MH, Ngwane AH, Wiid IJ, van Helden PD, Betoudji F, Nuermberger EL, Thomson JA. (2015) A Novel Inhibitor of Gyrase B is a Potent Drug Candidate for the Treatment of Tuberculosis and Non-Tuberculosis Mycobacteria. Antimicrob Agents Chemother. 59(3):1455-1465. (IF=4.476)
  • Farhat MR, Mitnick CD, Franke MF, Kaur D, Sloutsky A, Murray M, Jacobson KR. (2015) Concordance of Mycobacterium tuberculosis fluoroquinolone resistance testing: implications for treatment. Int J Tuberc Lung Dis. 19(3):339-341. (IF=2.315)
  • Fishbein S, van Wyk N, Warren RM, Sampson SL. (2015) Phylogeny to function: PE/PPE protein evolution and impact on Mycobacterium tuberculosis pathogenicity. Mol Microbiol. 96(5):901-916. (IF=4.419)
  • Fortuin S, Tomazella G, Nagayaryan N, Sampson S, Gey van Pittius N, Da Cruz Soares N, Wiker H, De Souza G, Warren RM. (2015) Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: Expanding the mycobacterial phosphoproteome catalogue. Front. Microbiol. 6:6. (IF=3.989)
  • Mandalakas AM, Kirchner HL, Walzl G, Gie RP, Schaaf HS, Cotton MF, Grewal HM, Hesseling AC. (2015) Optimizing the Detection of Recent Tuberculosis Infection in Children in a High TB-HIV Burden Setting. Am J Respir Crit Care Med. 191(7):820-830. (IF=12.996)
  • Daya M, van der Merwe L, van Helden PD, Möller M, Hoal EG. (2015) Investigating the Role of Gene-Gene Interactions in TB Susceptibility. PloS One. 10(4):e0123970. (IF=3.234)
  • Masson L, Passmore JA, Liebenberg LJ, Werner L, Baxter C, Arnold KB, Williamson C, Little F, Mansoor LE, Naranbhai V, Lauffenburger DA, Ronacher K, Walzl G, Garrett NJ, Williams BL, Couto-Rodriguez M, Hornig M, Lipkin WI, Grobler A, Karim QA, Karim SS. (2015) Genital Inflammation and the Risk of HIV Acquisition in Women. Clin Infect Dis. 61(2):260-269. (IF=8.886)
  • Mutai P, Pavadai E, Wiid I, Ngwane A, Baker B, Chibale K. (2015) Synthesis, antimycobacterial evaluation and pharmacophore modeling of analogues of the natural product formononetin. Bioorganic & Medicinal Chemistry Letters. 25:2510–2513. (IF=2.420)
  • van Rensburg IC, Loxton AG. (2015) Transcriptomics: the key to biomarker discovery during tuberculosis? Biomark Med. 9(5):483-495. (IF=2.646)
  • Axelsson-Robertson R, Rao M, Loxton AG, Walzl G, Bates M, Zumla A, Maeurer M. (2015) Frequency of Mycobacterium tuberculosis-specific CD8+ T-cells in the course of anti-tuberculosis treatment. Int J Infect Dis. 32:23-29. (IF=1.859)
  • Hesseling AC, Jaspan HB, Black GF, Nene N, Walzl G. (2015) Immunogenicity of BCG in HIV-exposed and non-exposed infants following routine birth or delayed vaccination. Int J Tuberc Lung Dis. 19(4):454-462. (IF=2.315)
  • Pietersen E, Peter J, Streicher E, Sirgel F, Rockwood N, Mastrapa B, Te Riele J, Davids M, van Helden P, Warren R, Dheda K. (2015) High frequency of resistance, lack of clinical benefit, and poor outcomes in capreomycin treated South african patients with extensively drug-resistant tuberculosis. PloS One. 10(4):e0123655. (IF=3.234)
  • Landolfi J, Terio K, Miller M, Junecko B, Reinhart T. (2015) Pulmonary Tuberculosis in Asian Elephants (Elephas maximus): Histologic Lesions With Correlation to Local Immune Responses. Vet Pathology. 52(3):535-542. (IF=1.869)
  • Viljoen IM, van Helden PD, Millar RP. (2015) Mycobacterium bovis infection in the lion (Panthera leo): Current knowledge, conundrums and research challenges. Veterinary Microbiology. 177(3-4):252-260. (IF=2.511)
  • Hamasur B, Bruchfeld J, van Helden PD, Källenius G, Svenson S. (2015) A sensitive urinary lipoarabinomannan test for tuberculosis in HIV negative patients. Plos ONE. 10(4):e0123457. (IF=3.234)
  • Buss P, Miller M, Hofmeyr J, Mathebula N, Kruger M, Bruns A, Martin L, Olea-Popelka F. (2015) Evaluation of cardiorespiratory, blood gas, and lactate values during extended immobilization of white rhinoceros (Ceratotherium simum). J Zoo and Wildlife Medicine. 46(2):224-233. (IF=0.424)
  • Sy S, de Kock L, Diacon A, Werely CJ, Xia H, Rosenkranz B, van der Merwe L, Donald PR. (2015) N-Acetyltransferase genotypes and the pharmacokinetics and tolerability of para-aminosalicylic acid in patients with drug-resistant pulmonary tuberculosis. AAC. 59(7):4129-4138. (IF= 4.476)
  • Van Halsema CL, Chihota VN, Gey van Pittius NC, Fielding KL, Lewis JJ, van Helden PD, Churchyard GJ, Grant AD. (2015) Clinical relevance of non-tuberculous mycobacteria isolated from sputum in a gold mining workforce in South Africa: an observational, clinical study. BioMed Research International. 959107:1-10. (IF=3.169)
  • le Roex N, Berrington CM, Hoal EG, van Helden PD. (2015) Selective breeding: The future of TB management in African buffalo? Acta Tropica. 149:38-44. (IF=2.270)
  • Cliff JM, Kaufmann SH, McShane H, van Helden P, O'Garra A. (2015) The human immune response to tuberculosis and its treatment: a view from the blood. Immunol Rev. 264(1):88-102. (IF=10.120)
  • Baumann R, Kaempfer S, Chegou NN, Oehlmann W, Spallek R, Loxton AG, van Helden PD, Black GF, Singh M, Walzl G. (2015) A Subgroup of Latently Mycobacterium tuberculosis Infected Individuals Is Characterized by Consistently Elevated IgA Responses to Several Mycobacterial Antigens. Mediators of Inflammation. e364758.  (IF=3.236)
  • Cliff JM, Cho JE, Lee JS, Ronacher K, King EC, van Helden P, Walzl G, Dockrell HM. (2016) Excessive Cytolytic Responses Predict Tuberculosis Relapse After Apparently Successful Treatment. J Infect Dis. 213(3):485-495. (IF=5.997)
  • Whitfield MG, Soeters HM, Warren RM, York T, Sampson SL, Streicher EM, van Helden PD, van Rie A. (2015) A Global Perspective on Pyrazinamide Resistance: Systematic Review and Meta-analysis. PloS One. 10(7):e0133869. (IF=3.234)
  • Whitfield MG, Warren RM, Streicher EM, Sampson SL, Sirgel FA, van Helden PD, Mercante A, Willby M, Hughes K, Birkness K, Morlock G, van Rie A, Posey JE. (2015) Mycobacterium tuberculosis pncA polymorphisms that do not confer pyrazinamide resistance at a breakpoint concentration of 100ug/ml in MGIT. JCM. 53(11):3633-3635. (IF=3.993)
  • Kayigire XA, Friedrich SO, van der Merwe L, Donald PR, Diacon AH. (2015) Simultaneous staining of sputum smears for acid-fast and lipid-containing Myobacterium tuberculosis can enhance the clinical evaluation of antituberculosis treatments. Tuberculosis. 95(6):770-779. (IF=2.711)
  • Masson L, Salkinder AL, Olivier AJ, McKinnon LR, Gamieldien H, Mlisana K, Scriba TJ, Lewis DA, Little F, Jaspan HB, Ronacher K, Denny L, Abdool Karim SS, Passmore JS. (2015) Relationship between female genital tract infections, mucosal IL-17 production and local Th17 cells. Immunology. 146(4):557-567. (IF=3.795)                              
  • Goosen WJ, Cooper D, Miller MA, van Helden PD, Parsons SD. (2015) IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer). Clin Vaccine Immunol. 22(8):974-978. (IF=2.470)
  • Haw A, Hofmeyr M, Buss P, Miller M, Meyer L, Fuller A, Fleming G. (2015) Evaluation of butorphanol combined with oxygen insufflation to improve cardiorespiratory function during chemical immobilization of free-ranging white rhinoceros (Ceratotherium simum).  J. S. Afr. Vet. Assoc.  86(1):1-10 pages. (IF= 0.350)
  • Miller M, Buss P, Olea-Popelka F, Wanty R, Parsons S, van Helden P. (2015) Baseline hematologic parameters for free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.  J. Wildl. Dis.  doi:10.7589/2015-03-081. (IF=1.355)
  • Streicher EM, Sampson SL, Dheda K, Dolby T, Simpson JA, Victor TC, Gey van Pittius NC, van Helden PD, Warren RM. (2015) Molecular epidemiological interpretation of the extensively drug resistant tuberculosis epidemic in South Africa. JCM. 53(11):3650-3653. (IF=3.993)
  • Corstjens PL, Tjon Kon Fat EM, de Dood CJ, van der Ploeg-van Schip JJ, Franken KL, Chegou NN, Sutherland JS, Howe R, Mihret A, Kassa D, van der Vyver M, Sheehama J, Simukonda F, Mayanja-Kizza H, Ottenhoff TH, Walzl G, Geluk A; AE-TBC consortium. (2016) Multi-center evaluation of a user-friendly lateral flow assay to determine IP-10 and CCL4 levels in blood of TB and non-TB cases in Africa. Clin Biochem. 49(1):22-31. (IF=2.275)
  • Salie M, Daya M, Lucas LA, Warren RM, van der Spuy GD, van Helden PD, Hoal EG, Möller M. (2015) Association of toll-like receptors with susceptibility to tuberculosis suggests sex-specific effects of TLR8 polymorphisms. Infect Genet Evol. 34:221-229. (IF=3.015)
  • Torres JN, Paul LV, Rodwell TC, Victor TC, Amallraja AM, Elghraoui A, Goodmanson AP, Ramirez-Busby SM, Chawla A, Zadorozhny V, Streicher EM, Sirgel FA, Catanzaro D, Rodrigues C, Gler MT, Crudu V, Catanzaro A, Valafar F. (2015) Novel katG mutations causing isoniazid resistance in clinical M. tuberculosis isolates. Emerg Microbes Infect. 4(7):e42. (IF=2.260)
  • Van Rie A, Warren RM. (2015) MDR tuberculosis control: time to change the dogma? Lancet. 3(12):907-909. (IF=45.217)
  • Losi M, Knights AJ, Mariani F, Altieri AM, Paone G, Loxton AG, Chegou NN, Kenneth J, Alma MG, Colizzi V, Walzl G, Saltini C, Boyle J, Amicosante M. (2016) QuantiFERON-TB performance enhanced by novel Mycobacterium tuberculosis-specific antigens. Europ Resp Journal. 47(2):660-664. (IF=7.636)
  • Theron G, Jenkins HE, Cobelens F, Abubakar I, Khan AJ, Cohen T, Dowdy DW. (2015) Data for action: collection and use of local data to end tuberculosis. Lancet. 386(10010):2324-2333. (IF=45.217)
  • van der Westhuyzen R, Winks S, Wilson CR, Boyle GA, Gessner RK, Soares de Melo C, Taylor D, de Kock C, Njoroge M, Brunschwig C, Lawrence N, Rao SP, Sirgel F, van Helden PD, Seldon R, Moosa A, Warner DF, Arista L, Manjunatha UH, Smith PW, Street LJ, Chibale K. (2015) Pyrrolo[3,4-c]pyridine-1,3(2H)-diones: A Novel Antimycobacterial Class Targeting Mycobacterial Respiration. J Med Chem. 58(23):9371-81. (IF=5.447)
  • Hammond-Aryee K, van Helden LS, van Helden PD. (2015) The prevalence of antibodies to Toxoplasma gondii in sheep in the Western Cape, South Africa. OVJ. 82(1):E1-5. (IF=1.256)
  • Dippenaar A, Parsons SD, Sampson SL, van der Merwe RG, Drewe JA, Abdallah AM, Siame KK, Gey van Pittius NC, van Helden PD, Pain A, Warren RM. (2015) Whole genome sequence analysis of Mycobacterium suricattae. Tuberculosis. 95(6):682-688. (IF=2.711)
  • Black PA, de Vos M, Louw GE, van der Merwe RG, Dippenaar A, Streicher EM, Abdallah AM, Sampson SL, Victor TC, Dolby T, Simpson JA, van Helden PD, Warren RM, Pain A. (2015) Whole genome sequencing reveals genomic heterogeneity and antibiotic purification in Mycobacterium tuberculosis isolates. BMC Genomics. 16(1):857. (IF=3.986)
  • Pule CM, Sampson SL, Warren RM, Black PA, van Helden PD, Victor TC, and Louw GE. (2016) Efflux pump inhibitors: targeting mycobacterial efflux systems to enhance TB therapy. J Antimicrob Chemother. 71(1):17-26. (IF=5.313)
  • Aas M, Blokland GA, Chawner SJ, Choi SW, Estrada J, Forsingdal A, Friedrich M, Ganesham S, Hall L, Haslinger D, Huckins L, Loken E, Malan-Müller S, Martin J, Misiewicz Z, Pagliaroli L, Pardiñas AF, Pisanu C, Quadri G, Santoro ML, Shaw AD, Ranlund S, Song J, Tesli M, Tropeano M, van der Voet M, Wolfe K, Cormack FK, DeLisi L. (2016) Summaries of plenary, symposia, and oral sessions at the XXII World Congress of Psychiatric Genetics, Copenhagen, Denmark, 12-16 October 2014. Psychiatr Genet. 26(1):1-47. (IF=1.941)
  • Esterhuyse MM, Weiner J 3rd, Caron E, Loxton AG, Iannaccone M, Wagman C, Saikali P, Stanley K, Wolski WE, Mollenkopf HJ, Schick M, Aebersold R, Linhart H, Walzl G, Kaufmann SH. (2015) Epigenetics and Proteomics Join Transcriptomics in the Quest for Tuberculosis Biomarkers. mBIO. 6(5):e01187-15. (IF=6.786)
  • Nicol MP, Gnanashanmugam D, Browning R, Click ES, Cuevas LE, Detjen A, Graham SM, Levin M, Makhene M, Nahid P, Perez-Velez CM, Reither K, Song R, Spiegel HM,Worrell C, Zar HJ, Walzl G. (2015) A Blueprint to Address Research Gaps in the Development of Biomarkers for Pediatric Tuberculosis. Clin Infect Dis. 61(suppl 3): S164-S172. (IF=8.886)
  • Kriel M, Lotz JW, Kidd M, Walzl G. (2015) Evaluation of a radiological severity score to predict treatment outcome in adults with pulmonary tuberculosis. Int J Tuberc Lung Dis. 19(11):1354-1360. (IF=2.315)
  • Schurz H, Daya M, Möller M, Hoal EG, Salie M. (2015) TLR1, 2, 4, 6 and 9 variants associated with tuberculosis susceptibility: a systematic review and meta-analysis. Plos One. 10(10):e0139711. (IF=3.234)
  • Theron G. (2015) How should laboratories measure bacterial load? S Afr Resp J. 21(3):54. (IF=Unknown)
  • Macuamule CL, Wiid IJ, van Helden PD, Tanner M, and Witthuhn RC. (2016) Effect of milk fermentation by kefir grains and selected single strains of lactic acid bacteria on the survival of Mycobacterium bovis BCG. Int J Food Microbiol. 217:170-176. (IF=3.082)
  • ElMaraachli W, Slater M, Berrada ZL, Lin SY, Catanzaro A, Desmond E, Rodrigues C, Victor TC, Crudu V, Gler MT, Rodwell TC. (2015) Predicting differential rifamycin resistance in clinical Mycobacterium tuberculosis isolates by specific rpoB mutations. Int J Tuberc Lung Dis. 19(10):1222-1226. (IF=2.315)
  • Miller M, Buss P, Wanty R, Parsons S, van Helden P, Olea-Popelka F. (2015) Baseline hematologic results for free-ranging white rhinoceros (Ceratotherium simum) in Kruger National Park, South Africa.  J Wildl Dis.  51(4):916-922. (IF=1.355)
  • Ticha LA, Klaasen JA, Green IR, Naidoo S, Baker B, Pietersen RD. (2015) Phytochemical and Antimicrobial Screening of Flavanones and Chalcones from Galenia africana and Dicerothamnus rhinocerotis. Nat Prod Commun. 10(7):1185-1190. (IF=0.906)
  • Lemmer Y, Kalombo L, Pietersen RD, Jones AT, Semete-Makokotlela B, Van Wyngaardt S, Ramalapa B, Stoltz AC, Baker B, Verschoor JA, Swai HS, de Chastellier C. (2015) Mycolic acids, a promising mycobacterial ligand for targeting of nanoencapsulated drugs in tuberculosis. J Control Release. 211:94-104. (IF=7.705)
  • Uys P, Brand H, Warren RM, van der Spuy GD, Hoal EG, van Helden PD. (2015) The risk of tuberculosis reinfection soon after cure of a first disease episode is extremely high in a hyperendemic community. Plos One. 10(12):e0144487. (IF=3.234)
  • Du Plessis WJ, Walzl G, Loxton AG. (2015) B cells as multi-functional players during Mycobacterium tuberculosis infection and disease. Tuberculosis. (2015) 1-8. (IF=2.711)
  • Dawson R, Diacon AH, Everitt D, van Niekerk C, Donald PR, Burger DA, Schall R, Spigelman M, Conradie A, Eisenach K, Venter A, Ive P, Page-Shipp L, Variava E, Reither K, Ntinginya NE, Pym A, von Groote-Bidlingmaier F, Mendel CM. (2015) Efficiency and safety of the combination of moxifloxacin, pretomanid (PA-824), and pyrazinamide during the first 8 weeks of antituberculosis treatment: a phase 2b, open-label, partly randomised trial in patients with drug-susceptible or drug-resistant pulmonary tuberculosis. Lancet. 385(9979):1738-1747. (IF=45.217)
  • Boeree MJ, Diacon AH, Dawson R, Narunsky K, du Bois J, Venter A, Phillips PP, Gillespie SH, McHugh TD, Hoelscher M, Heinrich N, Rehal S, van Soolingen D, van Ingen J, Magis-Escurra C, Burger D, Plemper van Balen G, Aarnoutse RE; PanACEA Consortium. (2015) A dose-ranging trial to optimize the dose of rifampin in the treatment of tuberculosis. Am J Respir Crit Care Med. 191(9):1058-1065. (IF=12.996)
  • Heinrich N, Dawson R, du Bois J, Narunsky K, Horwith G, Phipps AJ, Nacy CA, Aarnoutse RE, Boeree MJ, Gillespie SH, Venter A, Henne S, Rachow A, Phillips PP, Hoelscher M, Diacon AH; Pan African Consortium for the Evaluation of Antituberculosis Antibiotics (PanACEA); Pan African Consortium for the Evaluation of Antituberculosis Antibiotics PanACEA. (2015) Early phase evaluation of SQ109 alone and in combination with rifampicin in pulmonary TB patients. J Antimicrob Chemother. 70(5):1558-1566. (IF=5.313)
  • Diacon AH, Dawson R, von Groote-Bidlingmaier F, Symons G, Venter A, Donald PR, van Niekerk C, Everitt D, Hutchings J, Burger DA, Schall R, Mendel CM. (2015) Bactericidal activity of pyrazinamide and clofazimine alone and in combinations with pretomanid and bedaquiline. Am J Respir Crit Care Med. 191(8):943-953. (IF=12.996)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total:161)

Plenary/Keynote Lectures (11)

  • Walzl G. Challenges facing preclinical TB vaccine development. Aeras TB vaccine symposium, 46th Union World Conference on Lung Health, Cape Town, South Africa, 2-6 December 2015.
  • Walzl G, Ronacher K, Malherbe S, Chegou NN, Stanley K, van der Spuy GD, Kriel M, Kidd M, Warwick J, Barry CE 3rd, Chen R, Dodd L, Johnson JL, Boom WH, Peppard T, Cliff J, Dockrell H, Schoolnik G, Dolganov G, Alland D, Zak D, Winter J. Potential and limitations of current candidate host biomarkers for treatment response. Symposium: Spelunking for Treasure: Searching for Candidate Biomarkers as Prognostic Indicators for Treatment Outcome. 46th Union World Conference on Lung Health, Cape Town, South Africa, 2-6 December 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Plenary lecture, HHMI Science Meeting, Janelia Farm Research Campus, Ashburn, MD, 9-11 June 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Invited lecture, K-RITH, Durban, 26 June 2015.
  • Mizrahi V. MM4TB Project Update from Cape Town. MM4TB Consortium Meeting #10, Institut Pasteur, Paris, 29 June – 1 July 2015.
  • Mizrahi V. Targeting core metabolic pathways in M. tuberculosis. Plenary lecture, Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015.
  • Evans J, Trujillo C, Ehrt S, Boshoff HIM, Schnappinger D, Mizrahi V. A pathway approach to identifying vulnerable and bactericidal targets in Coenzyme A biosynthesis in M. tuberculosis. Plenary lecture, Gordon Research Seminar on Tuberculosis Drug Discovery & Development, Girona, Spain, July 11-12 2015.
  • Mizrahi V. Identifying vulnerable steps in the CoA pathway of M. tuberculosis. Plenary Lecture, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015.
  • Mizrahi V. Undertaking the full spectrum of research in a TB-endemic country: the example from South Africa. Plenary lecture, 46th Union World Conference on Lung Health, Cape Town, 5 December 2015.
  • Van Helden PD. Drug resistance and the contribution of zoonotic TB Plenary lecture, 46th Union World Conference on Lung Health, Cape Town, 2-6 December 2015.
  • Van Helden PD. Bovine TB in wildlife. Plenary Lecture, Workshop on Accelerating bTB Control in Developing Countries, Rabat, Morocco, 2-6 December 2015.

Invited Talks (81)

  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 9-11 June 2015. Annual meeting of the Howard Hughes Medical Institute. Janelia Farm Research Campus, Ashburn, Virginia, USA
  • Kana BD. Detection, quantification and characterization of differentially culturable tubercle bacteria in human TB disease. TB Biomarkers Annual Meeting. 3 – 5 June 2015, the Bill and Melinda Gates Foundation, Seattle, USA.
  • Kana BD. Differential bacterial growth states in active TB disease. “The New Post-2015 Global TB Strategy? The End Game”. 24 March 2015, The National Institute for Communicable Diseases, Sandringham, Johannesburg.
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 26 November 2015, The Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa.
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease. 1 October 2015, The Perinatal HIV Research Unit, Soweto, Johannesburg, South Africa
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease: Separation anxiety and schizophrenia in mycobacterial cells. 16 January 2015, The Kwazulu-Natal Institute for TB and HIV, Durban, South Africa.
  • Chegou N. Accuracy of a Seven-marker Host Serum Protein Biosignature in the Diagnosis of TB Disease in African Primary Health Care Clinic Attendees Presumed to have TB. 46th Union World Conference on Lung Health, Cape Town, 2 - 6 December 2015.
  • Chengalroyen M, Beukes G, Gordhan B, Neil Martinson, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral presentation, 15 – 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa
  • Chengalroyen M, Beukes B, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral Presentation, 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, Oral Presentation, October 2015, Postdoctoral Symposium, Wits University, Johannesburg, South Africa.
  • Gordhan B. The contribution of Nth and Nei DNA glycosylases to mutagenesis in Mycobacterium smegmatis. Oral Presentation, 15 – 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable and non-culturable bacteria in patients receiving first-line TB treatment, Oral presentation, 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Peters J, McIvor A, Papadopoulous A, Gordhan B, Kana B. Assessing the utility of Fatty acids in resuscitating DCTB from the sputum of TB infected patients. Oral and Poster. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • Peters J, McIvor A, Papadopoulous A, Gordhan B, Kana B. Establishing patterns in drug treatment response rates of tuberculosis disease based on non-culturable Mycobacterium tuberculosis in sputum. Oral presentation. 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable versus non-culturable bacteria in patients receiving first-line TB treatment: An endpoint analysis, Oral presentation, 24 July 2015, SoMCHAT Young Researchers conference, Chris Hani Baragwanath Hospital, Soweto, South Africa.
  • Ealand C, Kana B. DacB: An essential enzyme for mycobacterial growth, Poster with short talk, 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa
  • Ealand C, Kana B. An essential DD-carboxypeptidase determines localisation of peptidoglycan synthesis in mycobacteria, Oral, Health Sciences – Symposium for Postdoctoral and Carnegie Fellows, Johannesburg, South Africa.
  • Mizrahi V. Global health networks and networking. Invited lecture, Imperial-UCT Graduate Summer School, Global Health Fellows Programme, University of Cape Town, 26 January 2015.
  • Mizrahi V. The state of TB drug development and the role civil society can play. Treatment Action Campaign & Section 27 Symposium on Tuberculosis, Cape Town, 23 June 2015
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Talk presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Talk presented at the Pathology research day. FMHS, Stellenbosch University, Cape Town, South Africa, 04 June 2015.
  • Hoal EG. Genomic Disease Associations in TB in non-Euro-American Populations: The impact of ancestry. Talk presented at the NIAID Workshop on Host Response to TB-HIV Infection: A Genomic Perspective. NIAID, Rockville, USA, 14 January 2015.
  • Walzl G. Host biomarkers for active TB and for treatment response. South African Thoracic Society Conference, Cape Town, South Africa, 7-10 August 2015.
  • Walzl G. Deconfounding latent tuberculosis. South African Thoracic Society Conference, Cape Town, South Africa, 7-10 August 2015.
  • Walzl G. Host biomarkers for active TB and for treatment response. ACTG Annual Meeting, Washington DC, USA, June 2015
  • Walzl G. Immunodiagnostics of active TB disease. 4th Global Forum on TB Vaccines, Stop TB Partnership Working Group on New Vaccines. Shanghai, China, 21-24 April 2015.
  • Walzl G, Malherbe S, Ronacher K, Thiart L, Stanley K, van der Spuy GD, Kriel M, Warwick J, Barry CE 3rd, Peppard T, Alland D, Gregory Dolganov, Gary Schoolnik, Winter J. Human TB Treatment Response Studies Using PET/CT Imaging: Inconvenient Observations. Host Response in Tuberculosis- Keystone Symposia. Santa Fe, New Mexico, USA. January 22-27, 2015.
  • Warren RM. Genesis of drug resistance in Tuberculosis in South Africa. Plenary Talk presented at the PASER, HIVDR in the upcoming fight against antimicrobial resistance. Rainbow Towers Hotel, Harare, Zimbabwe, 08 May 2015.
  • Warren RM. The eveolving global TB epidemic- Focus on South Africa. Talk presented at the 5th Tuberculosis Control symposium. Education centre, The Woolcock, Glebe, Australia, 03-04 September 2015.
  • Klopper M. 1.Drug-resistance beyond XDR-TB: a health situation beyond repair? Talk presented at the Ukwanda Sustainable Rural Health Research Days. Worcester Campus, Stellenbosch University, Worcester, South Africa, 18-19 March 2015
  • Warren RM. Transmission of drug resistant TB- lessons from South Africa. Talk presented at the 5th Conference of the Union Asia Pacific Region. The Hilton Hotel, Sydney, Australia, 31 August - 02 September 2015.
  • Smith B. Application of Becton Dickinson FACSTM Combinatorial Antibody Profile (FACSTM CAP) technology to the identification of efficiency of tuberculosis therapy. Talk presented at the Stellenbosch University Annual Academic Year day. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Moller M. Population admixture: friend or foe in TB susceptibility. Talk presented at the 6th Biennial Congress of the Southern African Society of Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16 August - 19 September 2015.
  • le Roex N. Disease Control in Wildlife: BTB Test & Cull in African Buffalo. Talk presented at the International Congress of Conservation Biology (ICCB) 2015. Le Corum, Montpellier, France, 02-06 August 2015.
  • Moller M. Genome-wide association study of ancestry-specific TB risk in the South African Coloured population. Talk presented at the African Symposium on Genome Wide Association Studies for complex disease. African Institute for Mathematical Sciences of SA, Cape Town, South Africa, 23-24 April 2015.
  • Daya M. Using multi-way admixture mapping to elucidate TB susceptibility in the South African Coloured population. Talk presented at the African Symposium on Genome Wide Association Studies for complex disease. African Institute for Mathematical Sciences of SA, Cape Town, South Africa, 23-24 April 2015.
  • Klopper M. Next generation sequencing reveals hidden ethionamide resistance as a likely driver towards resistance beyond XDR-TB in a high burden population. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Tabb DL. Lipid Identification with Greazy and LipidLama. Talk presented at the Special Seminar for Department of Microbiology, Immunology, and Pathology. Colorado State University, Ft. Collins, CO, United States, 10 November 2015.
  • Whitfield M. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Whitfield M. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Kreiswirth B. Virtual sequencing of the entire pncA gene target in a single tube using LATE-PCR and Lights-On/Lights-Off probes to predict PZA susceptibility. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Kinnear C. Mutation Screening for Primary immunodeficiency disorders in a tuberculosis endemic region: a South African perspective. Talk presented at the 16th Biennial Congress of the South African Society for Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16-19 August 2015.
  • Kinnear C. Primary Immunodeficiency Disease management in tuberculosis endemic regions - are we aware -and how does a registry assist? Talk presented at the 4th African Society for Immunodeficiencies Congress. Hotel el Aurassi, Algiers, Algeria, 29-31 May 2015.
  • Sampson SL. Devastating Diseases and a Sustainable Society: Learning from the Tubercle Bacillus. Talk presented at the Lustrum conference, Science for Sustainability. Vrije University, Amsterdam, The Netherlands, 26 December 2015.
  • Sampson SL. Royal Society/Newton Fund International Exchanges Scheme. Talk presented at the Lustrum conference, Science for Sustainability. Vrije University, Amsterdam, The Netherlands, 26 November 2015.
  • Sampson SL. Single cell elucidation of mycobacterial replication dynamics. Talk presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Sampson SL. Single cell elucidation of mycobacterial replication dynamics. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Chegou NN. Host Serum Biosignatures for the diagnosis of TB disease. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 2-6 December 2015.
  • Willemse D. Rv1460 is required for growth of Mycobacterium tuberculosis in vitro. Talk presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Roos EO. Detecting Mycobacterial Infections in African Warthogs (Phacochoerus africanus) Using Serological Methods. Talk presented at the 6th Annual Wildlife Research Symposium. Onderstepoort, Pretoria, South Africa, 19-20 November 2015.
  • Theron G. Meet the Expert: Opportunities and challenges in TB diagnostic research: Overview and lessons learnt from two large diagnostic RCTs (Xpert and LAM). Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 06 December 2015.
  • Theron G. Point of care diagnostics for tuberculosis: where are we, and what is next?. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04 December 2015.
  • Theron G. Impact of Xpert MTB/RIF on TB case detection and treatment: experience in high-HIV settings. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Peter J, Theron G. Rapid point-of-care urine-based testing for tuberculosis and its impact on mortality: a multi-centre randomised controlled trial. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Lange B, Theron G. Diagnostic accuracy of the Xpert® MTB/RIF cycle threshold level to predict smear positivity in respiratory samples: a systematic review and meta-analysis. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Khaki A, Theron G. The effect of automated nucleic acid amplification assays on mortality in routine care settings: meta-analysis of individual participant data. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Loxton AG. TB immunology Research at Stellenbosch University Immunology Research Group. Annual ACTG Network Meeting, Washington, DC, 22 – 26 June 2015.
  • Pule C. Deciphering the physiology of drug tolerant and resistant Mycobacterium tuberculosis. Talk presented at the The 9th Annual Medical Research Council Early Career Scientist Conference. SAMRC conference centre, Cape Town, South Africa, 18-20 October 2015.
  • De Vos M. Rapid Single tube diagnosis of M(X)DR-TB. Talk presented at the Hain LifeSciences TB symposium. Holiday Inn, Harare, Zimbabwe, 25-26 March 2015.
  • De Vos M. The cutting edge of drug resistance diagnostics. Talk presented at the 6th FIDSSA congress. Champagne Sports Resort, Winterton, South Africa, 05-08 November 2015
  • De Vos M. Heterogeneity as revealed by whole genome sequencing in Mycobacterium tuberculosis. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • De Vos M. Construction and validation of a three colour single-tube assay for the detection of resistance to first- and second-line anti-TB antibiotics. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Ngwane A. Translational research in tuberculosis drug development. Talk presented at the Postdoctoral Research Symposium. Stellenbosch University, Cape Town, South africa, 29-30 September 2015.
  • Malherbe S. Evaluating TB treatment responses by [18F]FDG-PET/CT imaging – during and after treatment. 5th TB Biomarker Annual Meeting, Seattle, WA, 4 June 2015.
  • Miller MA. Elephant medicine and husbandry. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS. Continental Hotel, Canela, Brazil, 06 October 2015.
  • Miller MA. Tuberculosis in South African wildlife - why is it important? Talk presented at the Stellenbosch University Inaugural Lecture. FMHS, Stellenbosch University, Cape Town, South Africa, 23 June 2015.
  • Miller MA. Elephant medicine and husbandry. Invited Talk presented at the NIH Many Hosts of Mycobacteria Symposium. TNPRC, Covington, USA, 26 March 2015.
  • Miller MA. Why is diagnosis of TB so difficult? Talk presented at the BTB Outreach Day. Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa, 02 March 2015.
  • Miller MA. Tuberculosis diagnostic challenges in wildife and public health implications. Talk presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 05 December 2015.
  • Miller MA. Novel approaches to detection of tuberculosis in African wildlife. Talk presented at the US Animal Health Association annual conference. Providence Convention Center, Providence, USA, 26 October 2015.
  • Miller MA. Serodiagnostics for TB in wildlife species - can these play a role in detecting disease? Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Elephant TB - overview of clinical aspects, diagnosis, management and zoonotic risk. Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Warthog BTB - are they an underrecognized threat for BTB control?. Talk presented at the South African Veterinary Association Wildlife Group Congress. The Blades, Pretoria, South Africa, 20 March 2015.
  • Miller MA. Rumenitis and feed management in exotic ruminants. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Restraint, sedation, and immobilization of captive elephants and rhinos. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Hippopotamus medicine and immobilization. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 03 October 2015.
  • Miller MA. Field research in megavertebrate immobilization. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 05 October 2015.
  • Miller MA. Translating techniques used with free-ranging megavertebrates to zoo medicine. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015
  • Miller MA. Tuberculosis in wildlife in South Africa - are we winning the battle?. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015.
  • Miller MA. Diagnosis, management, and control of tuberculosis in captive elephants and rhinos. Talk presented at the 18th Congress of ABRAVAS and ALVEFAS, Workshop on Megavertebrate Medicine. Continental Hotel, Canela, Brazil, 04 October 2015.
  • Posters (69)
  • Senzani S, Bhaskar A, li D, Betzig E, Dhar N, Kana B. Identification and Characterization of Mycobacterial cell wall amidases. Oral and Poster. Swiss South Africa Joint Research Programme, Basel Switzerland.
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, School of Clinical Medicine Research Day. September 2015, Wits University, Johannesburg, South Africa
  • Chengalroyen M, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana B. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis, 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Moseki MR, Kana B. Functional analysis of cydDC-encoded type ABC transporter in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Nthambeleni GS, Kana B, Gordhan B. Is there a combined role for the Nth and MutY DNA glycosylases during DNA repair in Mycobacterium smegmatis? 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Hassim F, Papadopoulos AO, Kana BD, Gordhan BD. A combinatorial role for DNA glycosylases in mutation avoidance in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Papadopoulos A, Kana B. In silico analysis of M23-domain activators of peptidoglycan degrading amidases in Mycobacterium tuberculosis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Narrandes N, Kana B. Characterization of the electron transport chain in mycobacteria, 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z,Kana B. Detection of differentially culturable tubercle bacilli by exogenous cyclic-AMP in drug-susceptible patients at baseline,  15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • McIvor A, Gordhan B, Martinson N, Waja Z, Kana B. Detection of culturable and non-culturable TB bacteria in patients receiving first-line treatment, 9th Annual SAMRC Early Career Scientist Convention
  • Shaku M, Kana B. Characterization of LytM domain-containing proteins in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa
  • Maphatsoe MM, Ealana C, Kana B. Localization of low molecular weight proteins and other potential cell wall remodeling enzymes. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Rantsi TC, Kana B, Gordhan B. Molecular basis of the interplay between the Nth and the Nei DNA glycosylases in the base excision repair pathway in Mycobacterium smegmatis. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Sheik Ismail Z, Ealand C, Kana B. Characterization of mycobacterial DD- carboxypeptidases. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa. WON FIRST PRIZE
  • Sheik Ismail Z, Ealand C, Kana B. Characterization of mycobacterial DD- carboxypeptidases. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Ralefeta D, Machowski E, Kana B. Heterologous expression and characterisation of Mycobacterium tuberculosis DD-Carboxypeptidases in Mycobacterium smegmatis. 15 - 16 April, NHLS Pathology and Research Development Congress (PathRed), Johannesburg, South Africa.
  • Ralefeta D, Machowski E, Kana B. Mycobacterial DD-Carboxypeptidases: Filling In The Gaps. 3 December 2015, Molecular Biosciences Research Thrust (MBRT) Symposium, Wits University, Johannesburg, South Africa.
  • Ealand C, Kana B. An essential DD-carboxypeptidase determines localization of peptidoglycan synthesis in mycobacteria, Poster, 9th Annual SAMRC Early Career Scientist Convention.
  • Naran K. Construction and characterization of bioluminescent Mycobacterium tuberculosis reporter strains for mechanism of action studies in new TB drug discovery. Invited talk, K-RITH/IDM Scientific Symposium, Zimbali Lodge, Durban, 22-24 November 2015
  • Singh V. Identification and validation of GuaB2 as a tuberculosis drug target.  Invited talk, K-RITH/IDM Scientific Symposium, Zimbali Lodge, Durban, 22-24 November 2015
  • Evans J, Trujillo C, Ehrt S, Boshoff HIM, Schnappinger D, Mizrahi V. A pathway approach to identifying vulnerable and bactericidal targets in Coenzyme A biosynthesis in M. tuberculosis. Gordon Research Seminar on Tuberculosis Drug Discovery & Development, Girona, Spain, July 11-12 2015
  • Singh V, Pato J, Haartkorn R, Warner DF, Rizzi M, Keri G, Mizrahi V. Identification and validation of GuaB2 as a drug target. Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015
  • Mukherjee R, Ioerger T, Warner DF, Mizrahi V. Permeation in M. tuberculosis. Gordon Research Conference on Tuberculosis Drug Discovery & Development, Girona, Spain, 12-17 July 2015
  • Koch A, Brites D, Evans JC, Seldon R, Oni T, Nicol MP, Warner DF, Mizrahi V, Harris D, Parkhill J, Gagneux S, Martin DP, Wilkinson RJ. High Resolution Snapshot of Genetic Diversity within Mycobacterium tuberculosis in a Region of High HIV Co-Infection. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Mukherjee R, Ioerger T, Warner DF, Mizrahi V. Small hydrophilic molecule permeation in M. tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Broadley S, Warner DF, Sewell T. Structure determination of proteins involved in induced mutagenesis in Mycobacterium tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015
  • Singh V, Kigondu EM, Chibale K, Mizrahi V, Warner DF. Chlorpromazine potentiates the activity of spectinomycin against Mycobacterium tuberculosis. Poster, Biophysical Society Thematic meeting: Biophysics in the Understanding, Diagnosis and Treatment of Infectious Diseases, Spier Estate, Stellenbosch, South Africa, 16-20 November 2015.
  • Bunjun R, Rious C, Soares A, Hanekom W, Walzl G, Wilkinson R, Burgers W. Defects in multiple mycobacterial T helper subsets in blood and lungs in early HIV infection. Host Response in Tuberculosis- Keystone Symposia. Santa Fe, New Mexico, USA. 22-27 January 2015.
  • Corstjens PLAM, Tjon Kon Fat EM, Chegou NN, Ottenhoff THM, Walzl G, Geluk A. Assessment of lateral flow based assay in six African countries to determine IP-10 levels in stimulated whole blood samples of TB suspects. Host Response in Tuberculosis - Keystone Symposia. Santa Fe, New Mexico, USA. 22-27 January 2015.
  • Hammond-Aryee K. A high seroprevalence of Toxoplasma gondii antibodies in a population of feral cats in the Western Cape of South Africa. Poster presented at the13th International congress on Toxoplasmosis and Toxoplasma gondii research. Gettysberg College, Pennsylvania, USA, 17-20 June 2015.
  • Hammond-Aryee K. The prevalence of antibodies to Toxoplasma gondii in sheep in the Western Cape of South Africa. Poster presented at the13th International congress on Toxoplasmosis and Toxoplasma gondii research. Gettysberg College, Pennsylvania, USA, 17-20 June 2015.
  • Hammond-Aryee K. Genotypic characterization and strain diversity of Toxoplasma gondii from infected human and animal tissues from the Western Cape of South Africa. Poster presented at the59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Dippenaar A. Investigating microevolution in M. tuberculosis during transmission. Poster presented at the 36th annual Congress of the European Society of Mycobacteriology. Bellevue Park Hotel Riga, Riga, Latvia, 28 June - 01 July 2015.
  • Miller M, Parsons S, Buss P, Lyashchenko K, van Helden PD. Detection of immunological responses to BTB in African wildlife. Poster presented at the Keystone Symposium on Immunity to Veterinary Pathogens:  Informing Vaccine Development. Keystone resort, Colorado, USA, 20-25 January 2015.
  • Chegou, N. Diagnostic performance of a seven-marker serum protein biosignature for the diagnosis of active tb disease in African primary health care clinic attendees with suspected pulmonary tuberculosis. The Merck Africa Research Summit, in Geneva, Switzerland, from the 19-20 October 2015.
  • Da Camara NL. Large pncA deletions in Mycobacterium tuberculosis influence rapid detection of pyrazinamide susceptibility. Poster presented at the 5th Conference of The Union Asia Pacific Region. Hilton Hotel, Sydney, Australia, 31 August - 02 September 2015.
  • Da Camara NL. Targeted deep sequencing to detect heterogeneity in Mycobacterium tuberculosis populations. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kleynhans L, Ruzive S, Van der Spay G, Van Helden, PD, Walzl G, Ronacher, K. Evaluation of the endocrine changes during TB treatment. 4th European Congress of Immunology. Vienna, Austria, 6-9 September 2015.
  • Sao Emani C, Williams MJ, Wiid IJ, Baker B. Investigation of role of ergothioneine in Mycobacterium tuberculosis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Da Camara NL. Targeted deep sequencing of drug resistance associated genes to investigate heterogeneity in Mycobacterium tuberculosis populations. Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Parbhoo T, Mouton M, Sampson S. Optimization of Flow Cytometric Methods for Mycobacterial Viability Discrimination and Cell Enumeration. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kriel N, Heunis T, Williams M, Sampson S, Warren RM, van Helden PD. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Kriel N, Heunis T, Williams M, Sampson S, Warren RM, van Helden PD. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Kriel N, Heunis T, Sampson S, van Helden PD, Warren RM, Williams M. Global/High-throughput analysis of DNA-binding proteins in Mycobacterium smegmatis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Salie M, Daya M, Lucas LA, Warren RM, van der Spuy GD, van Helden PD, Hoal EG, Moller M. An investigation of toll-like receptors in tuberculosis susceptibility reveals sex-specific associations for TLR8 polymorphisms. Poster presented at the American Society of Human Genetics 2015 Annual Meeting. Baltimore Convention Centre, Baltimore, USA, 06-10 October 2015.
  • Kunsevi-Kilola C, Parbhoo T, Tshivhula H. Identification of distinct bio-signatures in whole blood and live M.tb stimulated PBMCs in TB house hold contacts with and without type 2 diabetes. Poster presented at the 1st IUIS-FAIS Southern African Immunology workshop and 6th Infectious Diseases in Africa Symposium. River Club, Cape Town, South Africa, 20-24 October 2015.
  • Salie M, Hoal EG. Investigation of TLR in TB susceptibility reveals sex-specific associations for TLR8. Poster presented at the ASHG conference. Baltimore conference centre, Baltimore, USA, 08 October 2015.
  • Daya M, Hoal EG. Using multi-way admixture mapping to elucidate TB susceptibility in the South african Coloured population. Poster presented at the ASHG conference. Baltimore conference centre, Baltimore, USA, 07 October 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Poster presented at the Southern African Society for Human Genetics. KleinKaap Boutique Hotel, Pretoria, South Africa, 16-19 August 2015.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with Primary Immunodeficiency Disorders. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the Postdoctoral Research Day. Stellenbosch University, Cape Town, South Africa, 29 October 2015.
  • Leisching G, Pietersen R-D, Mpongoshe V, van Heerden C, van Helden PD, Wiid I, Baker B. The host response to a clinical MDR Mycobacterial strain cultured in a detergent-free environment: A global transcriptomics approach. Poster presented at the 1st Biomedical Sciences Annual Research Day. FISAN Building, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Dippenaar A. Whole genome sequence analysis of Mycobacterium suricattae. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Whitfield M, Streicher EM, Mardarowicsz I, Scott L, Stevens W, Sampson SL,  van Helden PD, Warren RM, van Rie A. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Van Rie A, Whitfield M, Scott L, Warren RM, Voss De Lima Y, Stevens W, Sirgel F. Potential of Rifabutin in the Treatment of Rifampicin Resistant Tuberculosis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04-06 December 2015.
  • Visser H, de Vos M, van der Merwe RG, van Helden PD, Warren RM, Victor TC, Paul LV. Clofazimine: Mechanism of Resistance within M. tuberculosis. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 04 December 2015.
  • Sampson S, Mouton JM, Helaine S, Holden DW. Single cell elucidation of mycobacterial replication dynamics .Poster presented at the New Approaches and Concepts in Microbiology. EMBL, Heidelberg, Germany, 11-14 October 2015.
  • Chegou NN. Host biomarkers for the diangosis of TB disease. Poster presented at the UNESCO-Merck Africa Research Summit. Maotel Royal Hotel and Eden Palace Au Lac, Geneva, Switzerland, 19-20 October 2015.
  • Willemse D, Warren RM, Williams MJ. Generation and phenotypic characterisation of Rv1460 mutants of Mycobacterium tuberculosis. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Willemse D, Weber B, Warren RM, Williams MJ. Expression and purification of the Mycobacterium tuberculosis Rv1460, a possible suf system regulator. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Clarke C. Development of a Diagnostic Assay for Mycobacterium suricattae Infection in Meerkats (Suricata suricatta). Poster presented at the 1st Biomedical Sciences Annual Research Day. FMHS, Stellenbosch University, Cape Town, South Africa, 25 November 2015.
  • Roos EO. Diagnostic Tool Evaluation: Detection of Mycobacterial Infections in Warthogs (Phacochoerus africanus) Using Serological Tests. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 13 August 2015.
  • Theron G. Psychological distress and its relationship with nonadherence to TB treatment: a multicentre study. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 03 December 2015.
  • Calligaro G, Theron G, Khalfey H, Peter J, Miller M, Michell L, Joubert I, Dheda K. randomised controlled trial of the impact of Xpert® on tracheal aspirates within a burden of disease study in South African intensive care units. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Limberis J, Pietersen E, Jayakumar JJ, Theron G, Dheda K, Smith L, Warren RM, Clark T. Genetic markers of drug resistance and their association with clinical outcomes in patients with MDR-TB or XDR-TB. Poster presented at the 46th Union World Conference on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • De Vos M. Whole genome sequencing reveals genetic heterogeneity and suggests the role of selective bottleneck in defining the population structure of Mycobacterium tuberculosis clinical isolates. Poster presented at the 46th Union World Conferences on Lung Health. CTICC, Cape Town, South Africa, 02-06 December 2015.
  • Ngwane A, Baker B, Wiid I, van Helden PD. Design, synthesis of in vitro antitubercolosis activity of 2(5H) furanones. Poster presented at the 59th Annual Academic Day 2015. FMHS, Stellenbosch University, Cape Town, South Africa, 05 August 2015.
  • Kunsevi-Kilola C, Tshivula H. Characterization of human alveolar macrophage and blood monocyte derived macrophage responses to Mycobacterium tuberculosis in TB household contacts with and without type 2 diabetes. Poster presented at the 6th IDA Symposium & African Flow Cytometry Workshop. The River Club, Cape town, South Africa, 20-24 October 2015.

Products / Artifacts / Patents (2)

  • Method for diagnosing tuberculous meningitis.  Inventors: Chegou, NN, Walzl, G, van Furth, AM, Visser, DH, Applicant: Stellenbosch University. South African Provisional patent Application No: 2014/02743, Filing date: 2014/04/15. Worldwide (PCT) application was filed on the 15/04/2015 with application number: PCT/IB2015/052751, Status: Pending
  • Method for diagnosing tuberculosis. Inventors: Chegou, NN, Walzl, G, Mihret, A, Applicant: Stellenbosch University, Application type: PCT, claiming priority from the South African provisional patent: ZA 2014/01456, Country: PCT / WIPO Application No: PCT/IB2015/051435, Filing date: 2015/02/26, Status: Pending

 

2014

* The Names in bold are CBTBR staff and students
Books / Chapters in Books (Total: 3)

  • Du Plessis N, Walzl G. Helminth-M. TB co-infection. Adv Exp Med Biol. 2014; 828:49-74. doi: 10.1007/978-1-4939-1489-0_3. Review. PubMed PMID: 25253027.
  • Warner DF, Evans JC, Mizrahi,V. 2014. Nucleotide metabolism and DNA replication. In: Molecular Genetics of the Mycobacteria, 2nd Edition (Hatfull, G.F. & Jacobs, W.R., Jr., eds), ch. 30, pp. 635-656, ASM Press, Washington, D.C. Microbiol. Spectrum 2(5): MGM2-001-2013. doi: 10.1128/microbiolspec.MGM2-001-2013.
  • Warner DF. 2014 Mycobacterium tuberculosis metabolism. In: Tuberculosis: From bench to bedside (Rubin EJ, Kaufmann SHE & Zumla A, eds.). CSHL Press. doi: 10.1101/cshperspect.a021121.

Articles in Peer-Reviewed Journals (Total: 64)

  • Turapov O, Glenn S, Kana BD, Makarov V, Andrew PW, Mukamolova GV (2014) The In Vivo Environment Accelerates Generation of Resuscitation-Promoting Factor–Dependent Mycobacteria. American Journal of Respiratory and Critical Care Medicine; 190:1455-1457. (IF=11.99)
  • Martinson NA, McLeod KE, Milovanovic M, Msandiwa R, Lebina L. (2014) Implementation of isoniazid preventive therapy for HIV-infected adults: overstatement of district reports. Int. J. Tuberc. Lung. Dis. 18:1005. (IF=2.76)
  • Kana BD, Karakousis PC, Parish T, Dick T. (2014) Future target-based drug discovery for tuberculosis. Tuberculosis. 94. p.551-556. (IF=3.50)
  • Martinson NA, Gupte N, Msandiwa R, Moulton LH, Barnes GL, Ram M, Gray G, Hoffmann C, Chaisson RE. (2014) CD4 and viral load dynamics in antiretroviral-naïve HIV-infected adults from Soweto, South Africa: a prospective cohort. PLoS One 9:e96369. (IF=3.53)
  • Machowski EE, Senzani S, Ealand C, Kana BD. (2014) Comparative genomics for mycobacterial peptidoglycan remodelling enzymes reveals extensive genetic multiplicity. BMC Microbiol. 14. p75. (IF=2.98)
  • Moolla N, Goosens VJ, Kana BD, Gordhan BG. (2014) The contribution of Nth and Nei DNA glycosylases to mutagenesis in Mycobacterium smegmatis. DNA Repair (Amst). 13: 32-41. (IF=3.36)
  • Williams MJ, Mizrahi V, Kana BD. (2014) Molybdenum cofactor: a key component of Mycobacterium tuberculosis pathogenesis? Crit Rev Microbiol. 40(1): 18-29. (IF=6.09)
  • Koch A, Mizrahi V, Warner DF.  (2014) The physiological consequences of bacterial drug resistance: what can we learn from rifampicin? Emerg. Microbes Infect. 3: e17. (IF=Not available)
  • Mjambili F, Njoroge M, Naran K, Mizrahi V, Warner DF, Chibale K. (2014) Synthesis and biological evaluation of 2-aminothiazole derivatives as antimycobacterial and antiplasmodial agents. Biorg. Med. Chem. Lett. 24:560-564. (IF=2.33)
  • Xu Z, Wei Y, Marinelli LK, Evans JC, Chen J, Yu Y, Wilson DJ, Mizrahi V, Qiao C, Aldrich CC. (2014) Reaction intermediate analogues as bisubstrate inhibitors of pantothenate synthetase. Bioorg. Med. Chem. 22:1726-1735. (IF=2.95)
  • Arora K, Ochoa-Montano B, Tsang PS, Blundell TL, Dawes SS, Mizrahi V, Bayliss T, Mackenzie, CL, Cleghorn LAT, Ray PC, Wyatt PG, Uh E, Lee J, Barry CE III, Boshoff HI. (2014) Respiratory flexibility in response to inhibition of cytochrome c oxidase in M. tuberculosis. Antimicrob. Agents Chemother. 58(11):6962-6965. (IF=4.45)
  • Warner DF, Mizrahi V. (2014) Treatment shortening for tuberculosis – back to basics. New Engl. J. Med. 371:1642-1643. (IF=51.66)
  • Warner DF, Mizrahi V. (2014) Translation of genomics research into control of tuberculosis: lessons learned and future prospects. Genome Biol. 15:514. (IF=10.29)
  • Kigondu EM, Wasuna A, Warner DF, Chibale K. (2014) Pharmacologically active metabolites, combination screening and target identification-driven drug repositioning in antituberculosis drug discovery. Bioorg Med Chem. 22(16):4453-4461. (IF=2.33)
  • Singh K, Kumar M, Pavadai E, Naran K, Warner DF, Ruminski PG, Chibale K. (2014) Synthesis of new verapamil analogues and their evaluation in combination with rifampicin against Mycobacterium tuberculosis and molecular docking studies in the binding site of efflux protein Rv1258c. Bioorg. Med. Chem. Lett. 24(14):2985-2990. (IF=2.33)
  • Kigondu EM, Njoroge M, Singh K, Njuguna N, Warner DF, Chibale K. (2014) Synthesis and synergistic antimycobacterial screening of chlorpromazine and its metabolites. MedChemComm. 5: 502-506. (IF=2.63)
  • Baumann R, Jooste P, Chegou N, Oehlmann W, Loxton AG, Kaufmann SHE, Van Helden PD, Black G, Singh M, Walzl G. (2014) Serologic diagnosis of tuberculosis by combining Ig classes against selected mycobacterial targets. Journal of Infectious Diseases 69: 581-589. (IF=5.78)
  • Bekker A, Schaaf HS, Seifart HI, Draper HR, Werely CJ, Cotton MF, Hesseling AC. (2014) Pharmacokinetics of isoniazid in low-birth-weight and premature infants. Antimicrobial Agents and Chemotherapy 58(4): 2229-2234. (IF=4.45)
  • Black PA, Warren RM, Louw GE, Van Helden PD, Victor TC, Kana BD. (2014) Energy metabolism and drug efflux in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 58(5): 2491-2503. (IF=4.45)
  • Chegou N, Heyckendorf J, Walzl G, Lange C, Ruhwald M. (2014) Beyond the IFN-γ horizon: Biomarkers for immuno-diagnosis of infection with M. tuberculosis. European Respiratory Journal 43: 1472-1486. (IF=7.13)
  • Chimusa ER, Zaitlen N, Daya M, Möller M, Van Helden PD, Mulder NJ, Price AL, Hoal EG. (2014) Genome-wide association study of ancestry-specific TB risk. Human Molecular Genetics 23(3): 796-809. (IF=6.68)
  • Coetzee L, Nicol MP, Jacobson R, Schubert P, Van Helden PD, Warren RM, Wright CA. (2014) Rapid diagnosis of pediatric tuberculosis lymphadenitis utilizing fine needle aspiration biopsy. Pediatric Infectious Disease Journal 33(9): 893-896. (IF=3.14)
  • Daya M, Van Der Merwe L, Gignoux CR, Van Helden PD, Möller M, Hoal EG. (2014) Using multi-way admixture mapping to elucidate TB susceptibility in the South African Coloured population. BMC Genomics 15: 1021. (IF=4.04)
  • Daya M, Van Der Merwe L, Van Helden PD, Möller M, Hoal EG. (2014) The role of ancestry in TB susceptibility of an admixed South African population. Tuberculosis 94(4): 413-420. (IF=3.50)
  • Dheda K, Gumbo T, Gandhi NR, Murray M, Theron G, Udwadia Z, Migliori GB, Warren RM. (2014) Global control of tuberculosis: from extensively drug-resistant to untreatable tuberculosis. Lancet 2(4): 321-338. (IF=39.06)
  • Diacon AH, Pym A, Grobusch MP, De Los Rios JM, Gotuzzo E, Vasilyeva I, Leimane V, Andries K, Bakare N, De Marez T, Haxaiere-Theeuwes M, Lounis N, Meyvisch P, De Paepe E, Van Heeswijk R, Dannemann B. (2014) Multidrug-resistant tuberculosis and culture conversion with bedaquilline. New England Journal of Medicine 371(8): 723-731. (IF=51.66)
  • Diacon AH, Van Der Merwe L, Demers A, Von Groote-Bidlingmaier F, Venter A, Donald PR. (2014) Time to positivity in liquid culture predicts colony forming unit counts of Mycobacterium tuberculosis in sputum specimens. Tuberculosis 94: 148-151. (IF=3.50)
  • Diacon AH, Van Der Merwe L, Demers A, Von Groote-Bidlingmaier F, Venter A, Donald PR. (2014) Pre-treatment mycobacterial sputum load influences individual on-treatment measurements. Tuberculosis 94: 690-694. (IF=3.50)
  • Essone PN, Chegou N, Loxton AG, Stanley K, Kriel M, Van Der Spuy GD, Franken KL, Ottenhoff THM, Walzl G. (2014) Host Cytokine Responses Induced after Overnight Stimulation with Novel M. tuberculosis Infection Phase-Dependent Antigens Show Promise as Diagnostic Candidates for TB Disease. PLoS ONE 9(7): e102584. (IF=3.53)
  • Essone PN, Kalsdorf B, Chegou N, Loxton AG, Kriel M, Preyer R, Ersnt M, Walzl G, Lange C. (2014) Bifunctional T-cell-derived cytokines for the diagnosis of tuberculosis and treatment monitoring. Respiration 88(3): 251-261. (IF=2.92)
  • Garcia-Prats AJ, Willemse M, Seifart HI, Jordaan AM, Werely CJ, Donald PR, Schaaf HS. (2014) Acquired drug resistance during inadequate therapy in a young child with tuberculosis. Pediatric Infectious Disease Journal 33(8): 883-885. (IF=3.14)
  • Goosen WJ, Cooper D, Warren RM, Miller M, Van Helden PD, Parsons SDC. (2014) The evaluation of candidate biomarkers of cell-mediated immunity for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology 162(2-3): 198-202. (IF=1.75)
  • Goosen WJ, Miller M, Chegou N, Cooper D, Warren RM, Van Helden PD, Parsons SDC. (2014) Agreement between assays of cell-mediated immunity utilizing Mycobacterium bovis-specific antigens for the diagnosis of tuberculosis in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology 160(1-2): 133-138. (IF=1.75)
  • Gumbo T, Chigutsa E, Pasipanodya J, Visser ME, Van Helden PD, Sirgel FA, Mcilleron H. (2014) The pyrazinamide susceptibility breakpoint above which combination therapy fails. Journal of Analytical Chemistry 69(9): 2420-2425. (IF=0.81)
  • Hammond-Aryee K, Esser M, Van Helden PD. (2014) Toxoplasma gondii seroprevalence studies on humans and animals in Africa. South African Family Practice (Geneeskunde: The Medicine Journal) 56(2): 119-124. (IF=Not available)
  • Hammond-Aryee K, Esser M, Van Helden PD. (2014) Toxoplasmosis in South Africa - Old Disease in a New Context. Journal of Natural Sciences Research 4(22): 101-105. (IF=Not available)
  • Hillery N, Groessl EJ, Trollip A, Catanzaro D, Jackson L, Rodwell TC, Garfein RS, Lin G, Eisenach K, Ganiats TG, Park D, Valafar F, Rodrigues C, Crudu V, Victor TC, Catanzaro A. (2014) The Global Consortium for Drug-resistant Tuberculosis Diagnostics (GCDD): design of a multi-site, head-to-head study of three rapid tests to detect extensively drug-resistant tuberculosis. Trials 15: 434. (IF=2.12)
  • Hlokwe T, Van Helden PD, Michel AL. (2014) Evidence of increasing intra and inter-species transmission of Mycobacterium bovis in South Africa: Are we losing the battle? Preventive Veterinary Medicine 115(1-2): 10-17. (IF=2.51)
  • Hoshide M, Qian L, Rodrigues C, Warren RM, Victor TC, EvascoIi HB, Tupasi T, Crudu V, Douglas J. (2014) Geographical Differences Associated with SNPs in Nine Gene Targets among Resistant Clinical Isolates of Mycobacterium tuberculosis. Journal of Clinical Microbiology 58(5): 1322-1329. (IF=4.23)
  • Karinji MN, Esterhuizen TM, Friedrich SO, Diacon AH. (2014) Sputum volume predicts sputum mycobacterial load during the first two weeks of anti-tuberculosis treatment. Journal of Clinical Microbiology 1: 1-23. (IF=4.23)
  • Katale BZ, Mbugi EV, Botha L, Keyyu JD, Kendal S, Dockrell HM, Michel AL, Kazwala R, Rweyemamu M, Van Helden PD, Matee MLI. (2014) Species diversity of non-tuberculous mycobacteria isolated from humans, livestock and wildlife in the Serengeti ecosystem, Tanzania. BMC Infectious Diseases 14: 616. (IF=2.56)
  • Kaufmann SHE, Cotton MF, Eisele B, Gengenbacher M, Grode L, Hesseling AC, Walzl G. (2014) The BCG replacement vaccine VPM1002: from drawing board to clinical trial. Expert Review of Vaccines 13(5): 619-630. (IF=4.22)
  • Kibleur Y, Brochart H, Schaaf HS, Diacon AH, Donald PR. (2014) Dose regimen of Para-Aminosalicylic Acid Gastro-Resistant Formulation (PAS-GR) in multidrug-resistant Tuberculosis. Clinical Drug Investigation 34: 269-276. (IF=1.70)
  • Kolwijck E, Friedrich SO, Karinja MN, Van Ingen J, Warren RM, Diacon AH. (2014) Early stationary phase culture supernatant accelerates growth of sputum cultures collected after initiation of anti-tuberculosis treatment. Clinical Microbiology and Infection 20: 3. (IF=5.20)
  • Lin SY, Rodwell TC, Victor TC, Rider EC, Pham L, Catanzaro A, Desmond EP. (2014) Pyrosequencing for Rapid Detection of Extensively Drug-Resistant Tuberculosis in Clinical Isolates and Clinical Specimens. Journal of Clinical Microbiology 52(2): 475-482. (IF=4.23)
  • Marais E, Mlambo CK, Lewis JJ, Rastogi N, Zozio T, Grobusch MP, Duse AG, Victor TC, Warren RM. (2014) Treatment Outcomes of Multidrug-Resistant Tuberculosis Patients in Gauteng, South Africa. Infection 42(2): 405-413. (IF=2.86)
  • Marx FM, Dunbar R, Enarson DA, Williams BG, Warren RM, Van Der Spuy GD, Van Helden PD, Beyers N. (2014) The temporal dynamics of relapse and reinfection tuberculosis after successful treatment: a retrospective cohort study. Clinical Infectious Diseases 58(12): 1676-1683. (IF=9.42)
  • Mcgrath M, Gey Van Pittius NC, Sirgel FA, Van Helden PD, Warren RM. (2014) Moxifloxacin retains antimycobacterial activity in the presence of gyrA mutations. Antimicrobial Agents and Chemotherapy  58(5): 2912-2915. (IF=4.45)
  • Mcgrath M, Gey Van Pittius NC, Van Helden PD, Warren RM, Warner DF. (2014) Mutation rate and the emergence of drug resistance in Mycobacterium tuberculosis. Journal of Antimicrobial Chemotherapy 69(2): 292-302. (IF=4.45)
  • Mihret A, Loxton AG, Bekele Y, Kaufmann SHE, Kidd M, Haks MC, Ottenhoff THM, Aseffa A, Howe R, Walzl G. (2014) Combination of Gene Expression Patterns in Whole Blood Discriminate Between Tuberculosis Infection State. BMC Infectious Diseases 14: 257. (IF=2.56)
  • Nel HJ, Du Plessis N, Kleynhans L, Loxton AG, Van Helden PD, Walzl G. (2014) Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens. BMC Microbiology 14: 9. (IF=2.98)
  • Ota MO, Mendy JF, Donkor S, Togun T, Daramy M, Gomez MP, Chegou N, Sillah AK, Owolabi O, Kampmann B, Walzl G, Sutherland J. (2014) Rapid diagnosis of tuberculosis using ex vivo host biomarkers in sputum. European Respiratory Journal 44(1): 254-257. (IF=7.13)
  • Pietersen E, Ignatius E, Streicher EM, Mastrapa B, Padanilam X, Pooran A, Badri M, Lesosky M, Van Helden PD, Sirgel FA, Warren RM, Dheda K. (2014) Long-term outcomes of patients with extensively drug-resistant tuberculosis in South Africa: a cohort study. Lancet 6736(13): 1-10. (IF=39.21)
  • Ritter C, Lucke K, Sirgel FA, Warren RM, Van Helden PD, Böttger EC, Bloemberg G. Evaluation of the AID TB Resistance line probe assay for rapid detection of genetic alterations associated with Mycobacterium tuberculosis drug resistance. Journal of Clinical Microbiology 52(3): 940-946. (IF=4.23)
  • Rodwell TC, Valafar F, Douglas J, Qian L, Garfein RS, Chawla A, Torres J, Zadorozhny V, Kim MS, Hoshide M, Catanzaro D, Jackson L, Lin G, Desmond EP, Rodrigues C, Eisenach K, Victor TC, Ismail N, Crudu V, Gler MT, Catanzaro A. (2014) Predicting extensively drug-resistant tuberculosis (XDR-TB) phenotypes with genetic mutations. Journal of Clinical Microbiology 52(3): 781-789. (IF=4.23)
  • Roug A, Geoghegan C, Wellington E, Miller W, Travis E, Porter D, Cooper D, Clifford D, Mazet J, Parsons SDC. (2014) Utility of a fecal real-time PCR protocol for detection of Mycobacterium bovis infection in African buffalo Syncerus caffer. Journal of Wildlife Diseases 50(1): 140-142. (IF=1.31)
  • Salie M, Van Der Merwe L, Möller M, Daya M, Van Der Spuy GD, Van Helden PD, Martin MP, Goa X, Warren RM, Carrington M, Hoal EG. (2014) Associations between Human HLA Class-I Variants and the Mycobacterium tuberculosis Subtypes Causing Infection. Journal of Infectious Diseases 209(2): 216-223. (IF=5.48)
  • Streicher EM, Maharaj K, York T, Van Heerden CJ, Barnard M, Diacon AH, Mendel CM, Bosman ME, Hepple J, Pym A, Warren RM, Van Helden PD. (2014) Rapid sequencing of the Mycobacterium tuberculosis pncA gene for the detection of pyrazinamide susceptibility. Journal of Clinical Microbiology 52(11): 4056-4057. (IF=4.23)
  • Sutherland J, Loxton AG, Haks MC , Kassa D, Ambrose L, Lee JS, Ran L, Van Baarle D, Maertzdorf J, Howe R, Mayanja-Kizza H, Boom WH, Thiel BA, Crampin AC, Hanekom W, Ota MO, Dockrell HM, Walzl G, Kaufmann SHE, Ottenhoff THM. (2014) Differential gene expression of activating Fcγ receptor classifies active tuberculosis regardless of human immunodeficiency virus status or ethnicity. Clinical Microbiology and Infection 20(4): 230-238. (IF=5.20)
  • Todorov SD, Gombossy De Melo Franco B, Wiid IJF. (2014) In vitro study of beneficial properties and safety of lactic acid bacteria isolated from Portuguese fermented meat products. Beneficial Microbes 24: 1-16. (IF=1.50)
  • Van Crevel R, Dockrell HM, Eckold CE, Cliff JM, Moore D, Griffiths UK, Laurence YV, van Crevel R, Netea MG, Aarnoutse R, Lachmandas E, Kaufmann SH, Beigier-Bompadre M, Leitner S, Löwe D, Golinski R, Ottenhoff TH, Haks MC, Joosten SA, Vrieling F, Walzl G, Ronacher K, Malherbe S, van der Spuy G, Critchley JA, Kerry SR, Pearson F, Hill PC, McAllister SM, Ioana M, Panduru NM, Riza AL, Cimpoeru A, Nicoli R, Wijmenga C, Kumar V, Ugarte-Gil C, Lopez S, Coronel J, Alisjahbana B, Ruslami R, Soetedjo NN, Santoso P, Koesoemadinata RC, Schacht C, Büch J. (2014) TANDEM: understanding diabetes and tuberculosis. Lancet 2(4): 270-272. (IF=39.21)
  • Van Der Merwe RG, Van Helden PD, Warren RM, Sampson SL, Gey Van Pittius NC. (2014) Phage-based detection of bacterial pathogens. Analyst 139(11): 2617-2626. (IF=3.91)
  • Wallis RS, Dawson R, Friedrich SO, Venter A, Paige D, Zhu T, Silvia A, Gobey J, Ellery C, Zhang Y, Eisenach K, Miller P, Diacon AH. (2014) Mycobactericidal Activity of Sutezolid (PNU-100480) in Sputum (EBA) and Blood (WBA) of Patients with Pulmonary Tuberculosis. PLoS ONE  9(4): 1. (IF=3.53)
  • Zvada SP, Denti P, Sirgel FA, Chigutsa E, Hatherill M, Charalambous S, Mungofa S, Whitney K, Simonsson ASH, Jindani A, Harrison T, Mcilleron H. (2014) Moxifloxacin population pharmacokinetics and model-based comparison of efficacy between Moxifloxacin and Ofloxacin in African patients. Antimicrobial Agents and Chemotherapy 58(1): 503-510. (IF=4.45)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 118)

Plenary/Keynote Lectures

  • Kana BD. Academic Career Management. Plenary lecture presented at the SA PhD Project – KwaZulu Natal Regional Conference. Coastlands Convention Centre, Umhlanga Rocks. 18 – 19 November 2014.
  • Mizrahi V. Targeting pyrimidine metabolism in M. tuberculosis. Plenary lecture, Microbiology after the genomics revolution: Genomes 2014, EMBO Conference, Institut Pasteur, Paris, 24-27 June 2014.
  • Mizrahi V. Targeting core metabolic pathways in Mycobacterium tuberculosis. Plenary presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Falls, Zambia, 27-29 August 2014.
  • Mizrahi V. Targeting pyrimidine metabolism for TB drug discovery. Keynote lecture presented at the South African Immunology Society (SAIS) Conference, Cape Town, 03 February 2014.
  • Mizrahi V. The practice of research and publication in South Africa, Keynote lecture, UCT Libraries Research Week, Cape Town, 12 May 2014.
  • Streicher EM. The molecular war against drug resistant tuberculosis. Plenary speaker at the SACORE scientific meeting, Lilongwe, Malawi, 24-31 August 2014.
  • Invited Talks
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease: Separating anxiety and schizophrenia in mycobacterial cells. Invited lecture presented at the Weill Cornel Medical College, New York, USA, 9 September 2014
  • Kana BD. Peptidoglycan remodelling during mycobacterial cell division and tuberculosis disease: Separating anxiety and schizophrenia in mycobacterial cells. Invited lecture presented at the Rutgers Medical School, New Jersey, USA. 10 September 2014
  • Ealand CS, Kana BD. dacB: an essential enzyme for mycobacterial growth.  Oral presentation at the Wits Faculty of Health Sciences Research Day and Expo.  Johannesburg, South Africa,17 September 2014.
  • Ealand CS, Kana BD. dacB: an essential enzyme for mycobacterial growth.  Oral presentation at the 4th SA TB conference. International Convention Centre, Durban, South Africa, 10 – 13 June 2014.
  • Chengalroyen MD, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana BD. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis. Oral presentation at the 4th SA TB conference. International Convention Centre, Durban, South Africa, 10 – 13 June 2014.
  • Machowski E, Ealand CS, Senzani S, Kana BD. How Flexible Can Mycobacterial Cell Walls Be? A Bioinformatic Approach. Oral Presentation at the 4th South African TB Conference. Durban, South Africa, 10-13 June 2014.
  • Senzani S, Kana BD. Identification and Characterisation of Mycobacterial cell wall amidases. Oral presentation at the 4th SA TB conference. International Convention Centre, Durban, South Africa, 10 – 13 June 2014.
  • Senzani S, Kana BD. Identification and Characterisation of Mycobacterial cell wall amidases. Oral presentation at the 6th Cross Faculty Graduate Symposium 2014, University of the Witwatersrand, Johannesburg, 28 - 29 October 2014.
  • Senzani S, Kana BD. Identification and Characterisation of Mycobacterial cell wall amidases. Oral presentation at the Molecular Biosciences Research Thrust 2014 Symposium, 4th December 2014.
  • Beukes G, Chengalroyen M, Martinson N, Gordhan B, Kana BD. The effect of temperature on culture filtrates in stimulating bacterial culturability in tuberculous sputum. Oral presentation at the 4th SA TB conference. International Convention Centre, Durban, South Africa, 10 – 13 June 2014.
  • Narrandes NC, Kana BD. Characterisation of the mycobacterial electron transport chain: Implications for drug efficacy. Oral presentation at the 4th SA TB conference. International Convention Centre, Durban, South Africa, 10 – 13 June 2014.
  • Narrandes NC, Kana BD. Characterisation of the mycobacterial electron transport chain: Implications for drug efficacy. Oral presentation at the Biannual Faculty of Health Science Research Day and Postgraduate Symposium, 17 September 2014.
  • Sheik Ismail Z and Kana BD. Characterisation of mycobacterial DD-Carboxypeptidases. Poster and oral presentation at the 6th Cross Faculty Graduate Symposium 2014, University of the Witwatersrand, Johannesburg, 28 - 29 October 2014.
  • Mizrahi V. Tuberculosis drug development: lessons learned and future prospects. 36th Bernard Pimstone Memorial Lecture, Department of Medicine Research Day, University of Cape Town, 9 October 2014.
  • Mizrahi V. The IDM Today. Plenary lecture, Ten-Year Anniversary Symposium, Institute of Infectious Disease & Molecular Medicine, University of Cape Town, 2 November 2014.
  • Mizrahi V. Global health networks and networking. Invited lecture, Imperial-UCT Graduate Summer School, Global Health Fellows Programme, University of Cape Town, 26 January 2015.
  • Warner DF. Mechanisms of replication fidelity in Mycobacterium tuberculosis. South African Society for Biochemistry and Molecular Biology (SASBMB), Goudini Spa, 6-9 July, 2014.
  • Warner DF. A vitamin B12 shot for Mycobacterium tuberculosis. Invited lecture, K-RITH Seminar Series, KwaZulu-Natal Reseach Institute for TB and HIV (K-RITH), Durban, 25 April, 2014.
  • Warner DF. A vitamin B12 shot for Mycobacterium tuberculosis. Invited lecture, Department of Biomedical Sciences, Stellenbosch University. 2 June, 2014.
  • Ditse Z, Mizrahi V, Warner DF. Replication fidelity in the microevolution of Mycobacterium tuberculosis. 9th International Conference on the Pathogenesis of Mycobacterial Infections, Grand Hotel Saltsjӧbaden, Stockholm, Sweden. 25-29 June 2014.
  • Ditse Z, Mizrahi V, Warner DF. Replication fidelity in the microevolution of Mycobacterium tuberculosis. IDM Ten-Year Symposium, University of Cape Town, 2-4 November 2014.
  • Ditse Z, Mizrahi V, Warner DF. Replication fidelity in the microevolution of Mycobacterium tuberculosis. UCT-KRITH Retreat, Devon Valley Hotel, Stellenbosch 6-7 October 2014.
  • Broadley SG, Warner DF, Sewell BT. Structure determination of ImuC from Mycobacterium tuberculosis. Poster presented at the South African Society of Biochemistry and Molecular Biology conference.  Cape Town, 6-9 July 2014.
  • Chegou NN, Sutherland, JS, Mayanja-Kizza H, Howe R, Van der Vyver M, Kassa D, Crampin AC, Walzl G, AE-TBC Consortium. Utility of Mycobacterium tuberculosis-specific host cytokine signatures in whole blood culture supernatants in the diagnosis of Tuberculosis disease. Talk presented at the 7th EDCTP Forum, Theme: The partnership journey: New horizon for better health. 30 June – 2 July 2014.
  • Chegou NN, Loxton AG, Walzl G. Utility of M. tuberculosis specific host cytokine signatures in whole blood culture supernatants as diagnostic markers tuberculosis disease. Talk presented at the Seventh EDCTP Forum. The Partnership Journey: New Horizon for Better Health. Maritim proArte Hotel Berlin, Berlin, Germany, 28 June - 02 July 2014.
  • Loxton AG. TB Biomarkers: Diagnoses, treatment response and cure. Oral presentation at TB vaccine meeting, Les Diablerets, Switzerland 28-31 January 2014
  • Warren RM. Next Generation Sequencing: Applications in Tuberculosis. Invited talk presented at the NHLS Cape Town, South Africa, February 2014.
  • Warren RM. Emergence and spread of extensively and totally drug-resistant Mycobacterium tuberculosis. Invited talk presented at the ICAAC, Washington, USA, 7 September 2014.
  • Warren RM. Comparative Genomics of Drug-Resistant TB. Invited talk presented at the 4th TB Conference, Durban, South Africa, 10-13 June 2014.
  • Warren RM. Phenotypic and extended DST. Invited talk presented at the XDR-TB Workshop, UCT, Cape Town, South Africa, 10-12 October 2014.
  • Warren RM. Genesis of MDR and XDR-TB: Can we prevent this from happening in the future? Invited talk presented at the XDR-TB Workshop, UCT, Cape Town, South Africa, 10-12 October 2014.
  • Warren RM. Drug resistant tuberculosis: Creating the monster. Talk presented at the K-RITH Seminar series. K-RITH, University of KwaZulu-Natal, Durban, South Africa, 30 May 2014.
  • Sampson SL. In vivo imaging of Mycobacterium tuberculosis infection. Talk presented at the UK-SA Seminar on Imaging in Host-Pathogen Interactions. Cape Town, South Africa. November 2014.
  • Whitfield M, Streicher EM, York T, Mardarowicz I, Scott L, Stevens W, van Helden PD, Sampson S, Warren RM, Van Rie A. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium Tuberculosis Isolates. Talk presented at the 4th SA TB Conference 2014. Durban ICC, Durban, South Africa, 10-13 June 2014.
  • Whitfield MG, Streicher EM, York T, Mardarowicz I,  Scott L,  Stevens W,  van Helden PD,  Van Rie A,  Warren RM. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 9th International Conference on the Pathogenesis of Mycobacterial Infections. Grand Hotel Saltsjöbaden, Stockholm, Sweden, 26-29 June 2014.
  • Van Helden PD. Crossroads in TB research: where are we heading?. Talk presented at the 9th International Conference on the Pathogenesis of Mycobacterial Infections. Grand Hotel Saltsjobaden, Stockholm, Sweden, 26-29 June 2014.
  • Van Helden PD. Chalenging Dogmas using Molecular Biology to understand TB. Talk presented at the SASBMB Biennial Conference. Goudini Spa, Worcester, South Africa, 6-9 July 2014.
  • Whitfield MG, Streicher EM, York T, Mardarowicz I, Scott L, Stevens W, van Helden PD, van Rie A, Warren RM. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Talk presented at the 58th Annual Academic Day. Tygerberg, Cape Town, South Africa, 13 August 2014.
  • Malherbe ST. Measuring TB treatment response through PET/CT. Talk presented at South African Society of Nuclear Medicine 16th Biannual Congress. Maharani Hotel, Durban, South Africa, 21 September 2014.
  • Malherbe ST. TB treatment response. Talk presented at the The Spinal Surgery Masters Symposium. Protea Vinyard Hotel, Cape Town, South Africa, 01 November 2014.
  • Malherbe ST. Measuring Tb treatment response through PET/CT imaging. Talk presented at the H3-D TB drug development Symposium. Zambezi Sun, Livingstone, Zambia, 27-29 August 2014.
  • Malherbe ST. Measuring TB treatment responses through 18-FDG-PET/CT.Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Lucas L. Circulating MicroRNAs: Potential markers of early treatment response in pulmonary Tuberculosis. Talk presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Du Plessis J. The role of Bcells during Mtb infection. Talk presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Mgadi K. Role of monocytes in TB-Diabetes disease. Talk presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Streicher EM. Rapid prediction of Pyrazinamide resistance by pncA gene sequencing. Talk Presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Streicher EM. Shifting the Blame: Transmission vs Acquisition of XDR-TB. Talk Presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Pule C. The effect of efflux pump inhibitors on First and Second-Line anti-tuberculosis drugs differs in rifampicin mono-resistant clinical Isolates of Mycobacterium tuberculosis. Talk Presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Van der Merwe R. WGS pipeline applications to interrogate genetic diversity among clinical strains of Mycobacterium tuberculosis. Talk presented at the 4th South African TB Conference, Durban, South Africa, 10-13 June 2014.
  • Hammond-Aryee K. Toxoplasma gondii seroprevalence in humans and animals in Africa. Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • De Vos M. Construction of a Three Color Single-Tube Assay for Resistance to First, Second, and Third Line Antibiotics used to Diagnose and Treat XDR-TB. Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Van der Merwe R. A Whole Genome Sequencing approach to investigate the evolution of drug resistance in the Western Cape of South Africa. Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Aywoniyi D. Evaluation of cytokine responses against novel Mtb antigens as diagnostic markers for TB disease. Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Paul L. Validation of novel isoniazid resistance causing mutations in the KatG catalase-peroxidase of Mycobacterium tuberculosis. Talk presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Miller M. Mycobacterial infection and tuberculosis in free-ranging African lions (Panthera leo):  potential impact of environmental mycobacteria on diagnostic testing. Talk presented at the American Association of Zoo Veterinarians Conference, Orlando, USA, 19-24 October 2014.
  • Miller M. Effect of azaperone on blood pressure and other cardiorespiratory parameters in etorphine-immobilized free-ranging African elephants (Loxodonta africana) – role in managing anesthetic complications. Talk presented at the American Association of Zoo Veterinarians Conference, Orlando, USA, 19-24 October 2014.
  • Pule C. The effect of efflux pump inhibitors On First and Second-Line Anti-Tuberculosis Drugs Differs in Rifampicin Mono-Resistant Clinical Isolates of Mycobacterium Tuberculosis. Talk Presented at the European Society of Mycobacteriology 35th Annual Congress,Vienna, Austria, 29 June - 02 July 2014.
  • Miller M. Detection of immunological responses to Mycobacterium bovis in naturally infected African lions (Panthera leo) in Kruger National Park, South Africa. Talk presented at the V Annual Research Symposium in Wildlife Health, hosted by National Zoological Gardens, Pretoria, South Africa, 20-21 November 2014.
  • Streicher EM. Shifting the Blame: Transmission vs Acquisition of XDR-TB. Talk presented at the XDR-TB workshop UCT, Cape Town, South Africa, 10-12 October 2014.
  • Posters
  • Kana BD. Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidases play essential, non-redundant roles in growth and cell division in Mycobacterium smegmatis. Poster presentation at the Annual HHMI Scholars Meeting, 3-5 September 2014.
  • Kana BD. Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidases play essential, non-redundant roles in growth and cell division in Mycobacterium smegmatis. Poster presentation at the Gordon Research Conference on Bacterial Cell Surfaces, 22 - 27 June 2014.
  • Chengalroyen MD, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana BD. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis. Poster presentation at the Molecular Bioscienes Research Trust. University of Witwatersrand, South Africa, 4 December 2014.
  • Chengalroyen MD, Beukes G, Gordhan B, Martinson N, Otwombe K, Kana BD. The detection and quantification of differentially culturable bacilli in patients with active tuberculosis. Poster presentation at the Faculty of Health Sciences Biennial Research Day and Postgraduate Symposium. University of Witwatersrand, South Africa, 17 September 2014.
  • Senzani S and Kana BD. Identification and Characterisation of Mycobacterial cell wall amidases. Poster presentation at the Wits Health Science Research day and post graduate Expo 2014, Faculty of Health Science, University of the Witwatersrand, Johannesburg, 17 September 2014.
  • Beukes G, Chengalroyen M, Martinson N, Gordhan B, Kana BD. The effect of temperature on culture filtrates in stimulating bacterial culturability in tuberculous sputum. Poster presentation at the 6th Biennial Research Day and Postgraduate Expo 2014, Faculty of Health Science, University of the Witwatersrand, Johannesburg, 17  September  2014.
  • Beukes G, Chengalroyen M, Martinson N, Gordhan B, Kana BD. The effect of temperature on culture filtrates in stimulating bacterial culturability in tuberculous sputum. Poster presentation at the 6th Cross Faculty Graduate Symposium 2014, University of the Witwatersrand, Johannesburg, 28 - 29 October 2014.
  • Beukes G, Chengalroyen M, Martinson N, Gordhan B, Kana BD. The effect of temperature on culture filtrates in stimulating bacterial culturability in tuberculous sputum. Poster presentation at Molecular Biosciences Research Thrust 2014, University of the Witwatersrand, Johannesburg, 4 December 2014.
  • Sheik Ismail Z, Kana BD. DD-Carboxypeptidases affect crosslinking in mycobacteria. Poster presentation at the 4th Annual SA TB Conference, 2014, ICC, Durban, 10 – 13 June 2014.
  • Sheik Ismail Z, Kana BD. Characterisation of mycobacterial DD-Carboxypeptidases. Poster presentation at the 6th biennial Research day and Postgraduate Expo 2014, Faculty of Health Science, University of the Witwatersrand, Johannesburg, 17 September 2014.
  • Sheik Ismail Z, Kana BD. Characterisation of mycobacterial DD-Carboxypeptidases. Poster presented at the Molecular Biosciences Research Thrust 2014, University of the Witwatersrand, Johannesburg, South Africa, 4 December 2014.
  • Ralefeta D, Machowski E. and Kana BD. Heterologous expression and characterisation of Mycobacterium tuberculosis DD-Carboxypeptidases in Mycobacterium smegmatis. Poster presentation at the 6th Cross-Faculty Graduate Symposium 2014. University of the Witwatersrand, Johannesburg, South Africa, 28 - 29 October 2014.
  • Ralefeta D, Machowski E. and Kana BD. Heterologous expression and characterisation of Mycobacterium tuberculosis DD-Carboxypeptidases in Mycobacterium smegmatis. Poster presentation at the Molecular Biosiences Research Trust Research Day 2014. University of the Witwatersrand, Johannesburg, South Africa, 04 December 2014.
  • Kigondu E, Singh V, Warner DF, Chibale K. In vitro synergistic interactions and possible mechanism of action of chlorpromazine and its metabolites in combination with anti-TB drugs against Mycobacterium tuberculosis. Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Fals, Zambia, 27-29 August 2014.
  • Moosa A, Mizrahi V, Warner DF. Smart screening for TB drug discovery.  Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Falls, Zambia, 27-29 August 2014.
  • Naran K, Mizrahi V, Warner DF. An SOS-deficient mutant of Mycobacterium tuberculosis for use in phenotypic anti-TB drug screens Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Falls, Zambia, 27-29 August 2014.
  • Omollo C, Kigondu E, Warner DF, Chibale K. In vitro synergistic interactions of fusidic acid with known anti-tuberculosis drugs.  Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Falls, Zambia, 27-29 August 2014.
  • Wasuna A, Moosa A, Warner DF, Chibale K. Mutation in fusA1 causes in vitro resistance of Mycobacterium tuberculosis to fusidic acid. Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Falls, Zambia, 27-29 August 2014.
  • Evans JC, Abrahams GL, Mizrahi V. Identification of vulnerable steps in the Coenzyme A biosynthetic pathway of Mycobacterium tuberculosis. Poster presented at the Keystone Symposium on Novel Therapeutic Approaches to Tuberculosis. Keystone, Colorado USA, 30 March - 4 April 2014.
  • Broadley SG, Warner DF, Sewell BT. Structure determination of ImuC from Mycobacterium tuberculosis. Poster presented at the Higher European Research Course for Users of Large Experimental Systems. Grenoble, France, 23 February - 26 March 2014.
  • Broadley SG, Warner DF, Sewell BT. Structure determination of ImuC from Mycobacterium tuberculosis. Poster presented at the International Conference on the Crystallization of Biological Macromolecules. Hamburg, Germany, 17-20 September 2014.
  • Hammond-Aryee K. Toxoplasma gondii IgG Antibody Avidity testing at National Health Laboratory Services, Tygerberg Academic Hospital Cape Town. Poster presented at the African Society of Laboratory Medicine Conference, Cape Town, South Africa, 30 November - 04 December 2014.
  • Willemse D. Expression of the Mycobacterium tuberculosis SufR homologue. Poster presentation at the SASBMS conference, Cape Town, South Africa, 6-9 July 2014.
  • Black P. Novel mechanisms contrubuting to evolution of drug resistance in Mycobacterium tuberculosis. Poster presented at the 35th Annual Congress of the European Society of Mycobacteriology, Vienna, Austria, 29 June - 02 July 2014.
  • De Vos M. Construction of a Three Color Single-Tube Assay for Resistance to First, Second, and Third Line Antibiotics used to Diagnose and Treat XDR-TB. Poster presented at the 45th Union World Conference on Lung Health, Barcelona, Spain, 28 October - 01 November 2014.
  • De Vos M. Detecting resistance to nine anti Tuberculosis drugs in a single reaction tube. Poster presented at the 45th Union World Conference on Lung Health. Barcelona, Spain, 28 October - 01 November 2014.
  • Steyn N. Investigating the localisation of the ESX-3 secretion system in Mycobacterium smegmatis. Poster presented at the 52nd Annual Microscopy Society of Southern Africa (MSSA 2014) hosted by Stellenbosch University, Cape Town, South Africa, 2-5 December 2014.
  • Mouton J. Single cell elucidation of mycobacterial replication dynamics. Poster presented at the 52nd Annual Microscopy Society of Southern Africa (MSSA 2014) hosted by Stellenbosch University, Cape Town, South Africa, 2-5 December 2014.
  • Hammond-Aryee K. Current Toxoplasma gondii seroprevalence studies in humans and animals in the western cape and policy implications. Poster presented at the 58th Annual Academic Day, Stellenbosch Univerrsity, Cape Town, South Africa, 13 August 2014.
  • Klopper M. Deciphering the Evolution of Totally Drug-Resistant tuberculosis: A catalogue of genome variation at single-base pair resolution. Poster presented at the 58th Annual Academic Day, Stellenbosch Univerrsity, Cape Town, South Africa, 13 August 2014.
  • Black P. Novel mechanisms contrubuting to evolution of drug resistance in Mycobacterium tuberculosis. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Fang Z. Functional study of Mycosin-3, an essential subtilisin-like serine protease in Mycobacterium tuberculosis - A proteomic approach. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Bowker N. Detection of novel TB susceptibility variants using next generation sequencing of the Major Histocompatibility and Leukocyte Receptor Complexes. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Goosen WJ. The Use Of Mycobacterium Bovis-Specific Antigens For The Diagnosis Of Tuberculosis In African Buffaloes (Syncerus Caffer).. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Kayigire X. Quantification of viable Mycobacterium tuberculosis using propidium mononazide combined with Xpert MTB/RIF. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • McFayden R. Investigating the Humoral Immune Response to M. bovis Infection in African Buffaloes (Syncerus caffer). Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Sao Emani C. Ergothioneine is secreted by slow growing mycobacteria. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Schlechter N. Identification of novel candidate genes for susceptibility to tuberculosis by identifying disease-causing mutations in individuals with PIDs. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Theys M. Characterising two essential penicillin-binding proteins of Mycobacterium tuberculosis. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Visser H. Deciphering the Resistance Mechanisms of Mycobacterium tuberculosis to Clofazimine. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Uren C. Fine-scale population structure between the Nama and Khomani San of Southern Africa. Poster presented at the 58th Annual Academic Day, Stellenbosch University, Cape Town, South Africa, 13 August 2014.
  • Fortuin S. Whole genome sequencing and Proteomic analysis of a heteroresistant Mycobacterium tuberculosis clinical isolate suggest in vitro evolution lead to an altered metabolic state of the rpoB resistant strain. Poster presented at the SACORE scientific meeting, Lilongwe, Malawi, 24-31 August 2014.
  • Miller M. Use of rapid serological assays for detection of M. bovis in free-ranging African lions (Panthera leo). Poster presented at the VI International M. bovis Conference, Cardiff, Wales, 16-19 June 2014.
  • Fortuin S. Whole genome sequencing and proteomic analysis of a heteroresistant Mycobacterium tuberculosis clinical isolate suggest in vitro evolution lead to an altered metabolic state of the rpoB resistant strain. Poster presented at the Wellcome Trust Emerging Scientific Researchers Conference, Uganda, 19-24 May 2014.
  • Uren C. Fine-scale population structure between the Nama and Khomani San of southern Africa. Poster presented at the Society of Molecular Biology and Evolution Annual Conference. Hilton Hotel, San Juan, Puerto Rico, 8-12 June 2014.
  • Uren C. Investigating fine-scale population structure between the Nama and Khomani San of southern Africa. Poster presented at the SASBi-SAGS Joint Congress. Kwalata Game Ranch, Tswane, South Africa, 23-26 September 2014.
  • Kleynhans L, Ronacher K, Walzl G, Loxton AG, Malherbe ST. Evaluation of the endocrine changes during TB treatment. Poster presented at the H 3-D Drug Discovery & Development:  Innovative approaches to TB drug discovery: Laboratory to Bedside. Zambezi Sun, Livingston, Zambia, 27-29 August 2014.
  • Williams MJ. Biosynthesis of bis-molybdopterin guanine dinucleotide is dispensable for virulence of Mycobacterium tuberculosis in mice. Poster presented at the South African Society for Biochemistry and Molecular Biology Conference. Guidini Spa, Cape Town, South Africa, 6-9 July 2014.
  • Lucas LA, Walzl G, du Plessis J, Mgadi K. Circulating MicroRNAs: Potential Markers of Early Treatment Response in Pulmonary Tuberculosis. Poster presented at the 4th SA TB Conference. Durban ICC, Durban, South Africa, 10-13 June 2014.
  • Salie M, van der Merwe L, Möller M, Daya M, van der Spuy G, van Helden PD, Warren R, Hoal EG. Associations Between Human Leukocyte Antigen Class I Variants and the Mycobacterium tuberculosis Subtypes Causing Disease. Poster presented at the IUATLD. Barcelona Conference Centre, Barcelona, Spain, 30 October 2014.
  • Whitfield MG, Streicher EM, York T, Mardarowicz I, Scott L, Stevens W, Van Helden PD, Van Rie A, Warren RM. Association between genotypic and phenotypic pyrazinamide resistance in Mycobacterium tuberculosis. Poster presented at the 16th International Congress on Infectious Diseases. CTICC, Cape Town, South Africa, 02-05 April 2014.
  • Kigondu E, Singh V, Warner DF, Chibale K. In vitro synergistic interactions and possible mechanism of action of chlorpromazine and its metabolites in combination with anti-TB drugs against Mycobacterium tuberculosis. Poster presented at the H3-D Symposium on Innovative Approaches to TB Drug Discovery: from Bench to Bedside. Victoria Fals, Zambia, 27-29 August 2014.
  • De Vos M, Rice J, Rice L, Kreiswirth B, Kurepina N, Streicher EM, van Helden PD, Warren RM, Wangh  L. Evaluation and Validation of a Highly Multiplexed  LATE-PCR Single-tube Assay for M(X)DR-TB. Poster presented at the 16th International Congress of Infectious Dieseases. CTICC, Cape Town, South Africa, 02-05 April 2014.
2013

* The Names in bold are CBTBR staff
Books / Chapters in Books (Total: 0)
Articles in Peer-Reviewed Journals (Total: 56)

  • Gous N, Cunningham B, Kana BD, Stevens W, Scott LE. (2013) Performance monitoring of Mycobacterium tuberculosis Dried Culture Spots for use on the GeneXpert within a National Program in South Africa. J. Clin. Microbiol. 51:4018-4021. (IF=4.07)
  • Small JL, Park SW, Kana BD, Ioerger TR, Sacchettini JC, Ehrt S. (2013) Perturbation of cytochrome c maturation reveals adaptability of the respiratory chain in Mycobacterium tuberculosis. MBio, 4:e00475-13. (IF=5.62)
  • Li Y, de Kock C, Smith PJ, Hendricks DT, Naran K, Mizrahi V, Warner DF, Chibale K, Smith GS. (2013) Synthesis, characterization, and pharmacological evaluation of silicon-containing aminoquinoline organometallic complexes as antiplasmodial, antitumor, and antimycobacterial agents. Organometallics 32:141-150. (IF=4.15)
  • Gopinath K, Venclovas Č, Ioerger T, Sacchettini JC, McKinney JD, Mizrahi V, Warner DF.  (2013) An ABC transporter essential for vitamin B12 acquisition in Mycobacterium tuberculosis. Open Biology 3:120175. (IF=3.63)
  • Warner DF, Tonjüm T, Mizrahi V. (2013) DNA metabolism in mycobacterial pathogenesis. Curr. Top. Microbiol. Immunol.doi: 10.1007/82_2013_328. (IF=4.86)
  • Gopinath K, Moosa A, Mizrahi V, Warner DF. (2013) Vitamin B12 metabolism in mycobacteria. Future Microbiol. 8:1405-1418. (IF=4.02)
  • Kumar A, Casey A, Odingo J, Kesicki EA, Abrahams GL, Vieth M, Masquelin T, Mizrahi V, Hipskind PA, Sherman DR, Parish T. (2013) A high-throughput screen against pantothenate synthetase (PanC) identifies 3-biphenyl-4-cyanopyrrole-2-carboxylic acids as a new class of inhibitor with activity against Mycobacterium tuberculosis. PLoS One 8:e72786. (IF=3.73)
  • Ioerger T, O’Malley T, Liao R, Guinn K, Hickey M, Mohaideen N, Murphy K, Boshoff H, Mizrahi V, Rubin EJ, Sassetti CM, Barry CE III, Sherman D, Parish T,  Sacchettini JC. (2013) Identification of new drug targets and resistance mechanisms in Mycobacterium tuberculosis. PLoS One8:e75245. (IF=3.73)
  • Warner DF, Mizrahi V. (2013) Complex genetics of drug resistance in Mycobacterium tuberculosis. Nat. Genet. 45:1107-1108. (IF=35.21)
  • Hoffman AE, Destefano M, Shoen C, Gopinath K, Warner DF, Cynamon M, and Doyle RP. (2013) Co(II) and Cu(II) pyrophosphate complexes have selectivity and potency against Mycobacteria including Mycobacterium tuberculosis. Eur. J. Med. Chem. 70:589-593. (IF=3.50)
  • Axelsson-Robertson R, Loxton AG, Walzl G, Ehlers MM, Kock MM, Zumla A, Maeurer M. (2013) A Broad Profile of Co-Dominant Epitopes Shapes the Peripheral Mycobacterium tuberculosis Specific CD8+ T-Cell Immune Response in South African Patients with Active Tuberculosis. PLoS One. 2013; 8(3):e58309. (IF=3.73)
  • Ansong CK, Payne SH, Haft DH, Corrie O, Chauvigné-Hines LM; Purvine SO; Shukla AK; Fortuin S, Smith RD, Adkins JN, Grundner C, Wright AT. (2013) Novel and Widespread Adenosine Nucleotide-Binding in Mycobacterium tuberculosis. Chem Biol. 20(1):123-133. (IF=6.16)
  • Ayles H, Muyoyeta M, du Toit E, Schaap A, Floyd S, Simwinga M, Shanaube K, Chichinga N, Bond V, Dunbar R, de Haas P, James A, Gey van Pittius NC, Claassens M, Fielding K, Fenty J, Sismanidis C, Hayes R, Beyers N, Godfrey-Faussett P and the ZAMSTAR team (including PD van Helden). 2013 Effect of household and community interventions on the burden of tuberculosis in southern Africa: the ZAMSTAR community-randomised trial. Lancet. 382(9899):1183-1194. (IF=39.06)
  • Baumann R, Kaempfer S, Chegou NN, Nene NF, Veenstra H, Spallek R, Bolliger C, Lukey P, van Helden PD, Singh M, Walzl G. (2013) Serodiagnostic markers for the prediction of the outcome of intensive phase tuberculosis therapy. Tuberculosis. 93(2):239-245. (IF=3.03)
  • Beatty KE, Williams M, Carlson BL, Swarts BM, Warren RM, van Helden PD, Bertozzi CR. (2013) Sulfatase-Activated Fluorophores for Rapid Discrimination of Mycobacterial Species and Strains. PNAS. 110(32):12911-12916. (IF=9.74)
  • Borrell S, Teo Y, Giardina F, Streicher EM, Klopper M, Feldmann J, Müller B, Victor TC, Gagneux S. (2013)  Epistasis between antibiotic resistance mutations drives the evolution of extensively drug-resistant  tuberculosis. Evolution, Medicine and Public Health. doi:10.1093/emph/eot003 (IF=Not available)
  • Botha L, Gey van Pittius NC, van Helden PD. (2013) Mycobacteria and disease in Southern Africa. Trans Emerg Dis. 60:147-156. (IF=2.10)
  • Bryant J, Harris SR, Parkhill J, Dawson R, Diacon AH, Van Helden PD, Pym A, Mahayiddin AA, Chuchottaworn C, Sanne IM, Louw C, Boeree MJ, Hoelscher M, Mchugh TD, Bateson A, Hunt RD, Mwaigwisya S, Wright L, Gillespie SH, Bentley SD. (2013) Whole genome sequencing of Mycobacterium tuberculosis for determination of relapse or reinfection. Lancet 1(10): 786-792. (IF=39.06)
  • Chegou NN, Detjen AK, Thiart L, Walters E, Mandalakas AM, Hesseling AC, Walzl G. (2013) Utility of host markers detected in Quantiferon supernatants for the diagnosis of tuberculosis in children in a high -burden setting. PLoS One. 8(5): e64226. (IF=3.73)
  • Chihota VN, van Halsema CL, Grant AD, Fielding KL, van Helden PD, Churchyard GJ, Gey van Pittius NC. (2013) Spectrum of non-tuberculous mycobacteria identified using standard biochemical testing versus 16S sequencing. IJTLD. 17(2):236-269. (IF=2.61)
  • Claassens M, van Schalkwyk C, den Haan L, Floyd S, Dunbar R, van Helden PD, Godfrey-Faussett P, Ayles H, Borgdoff M, Enarson D, Beyers N. (2013) High Prevalence of Tuberculosis and Insufficient Case Detection in Two Communities in the Western Cape, South Africa. Plos One. 8(4): e58689. (IF=3.73)
  • Cliff JM, Lee JS, Constantinou N, Cho JE, Clark T, Ronacher K, King EL, Lukey PT, Duncan K, van Helden PD, Walzl G, Dockrell HM. (2013) Distinct phases of blood gene expression pattern through tuberculosis treatment reflect modulation of the humoral immune response. J Infect Dis. 207(1):18-29. (IF=5.85)
  • Cobat A, Hoal EG, Gallant CJ, Simkin L, Black GF, Stanley K, Jais JP, Yu TH,  Boland-Auge A, Grange G, Delacourt C, van Helden PD, Casanova JL, Abel L, Alcais A, Schurr E. (2013) Identification of a major locus, TNF1, that controls BCG-triggered TNF production by leukocytes in an area hyperendemic for tuberculosis. Clin Infect Dis. 57(7):963-970. (IF=9.37)
  • Diacon AH, Dawson R, Von Groote-Bidlingmaier F, Symons G, Venter A, Donald PR, Conradie A, Erondu N, Ginsberg AM, Egizi E, Winter H, Becker P, Mendel CM (2013): Randomized dose-ranging study of the 14-day early bactericidal activity of bedaquiline (TMC207) in patients with sputum microscopy smear-positive pulmonary tuberculosis. Antimicrob Agents Chemother. 57(5):2199-2203. (IF=4.57)
  • De Vos M, Muller B, Borrell S, Black PA, van Helden PD, Warren RM, Gagneux S, Victor TC. (2013) Putative compensatory mutations in the rpoC gene of rifampicin-resistant Mycobacterium tuberculosis are associated with ongoing transmission. Antimicrobial Agents and Chemotherapy. 57(2):827-832. (IF=4.57)
  • Du Plessis N, Kleynhans L, Thiart L, van Helden PD, Brombacher F, Horsnell WGC, Walzl G. (2013) Acute helminth infection enhances early macrophage mediated control of mycobacterial infection. Mucosal Immunology. 6(5):931-941. (IF=7.01)
  • Du Plessis N, Loebenberg L, Kriel M, von Groote-Bidlingmaier F, Ribechini E, Loxton AG, van Helden PD, Lutz MB, Walzl G. (2013) Increased frequency of myeloid-derived suppressor cells during active tuberculosis and after recent mycobacterium tuberculosis infection suppresses T-cell function. AJRCCM. 188(6):724-732. (IF=11.04)
  • Durr S, Muller B, Alonso S, Hattenhorf J, Laisse C, van Helden PD, Zinsstag J. (2013) Differences in primary sites of infection between zoonotic and human tuberculosis: Results from a worldwide systematic review. PLoS Negl Trop Dis. 7(8):e2399. (IF=4.57)
  • Farhat MR, Shapiro BJ, Kieser KJ, Sultana R, Jacobson KR, Victor TC, Warren RM, Streicher EM, Calver A, Sloutsky A, Kaur D, Posey JE, Plikaytis B, Oggioni MR, Gardy JL, Johnston JC, Rodrigues M, Tang PK, Kato-Maeda M, Borowsky ML, Muddukrishna B, Kreiswirth BN, Kurepina N, Galagan J, Gagneux S, Birren B, Rubin EJ, Lander ES, Sabeti PC, Murray M. (2013) Genomic analysis identifies targets of convergent positive selection in drug-resistant Mycobacterium tuberculosis. Nat Genet. 45(10):1183-1189. (IF=35.21)
  • Friedrich SO, Rachow A, Saathoff E, Singh K, Mangu CD, Dawson R, Phillips PPJ, Venter A, Bateson A, Boehme CC, Heinrich N, Hunt RD, Boeree MJ, Zumla A, McHugh TD, Gillespie SH, Diacon AH, Hoelscher M, on behalf of the Pan African Consortium for the Evaluation of Anti-tuberculosis Antibiotics (PanACEA) (2013): Assessment of the sensitivity and specificity of Xpert MTB/RIF assay as an early sputum biomarker of response to tuberculosis treatment. Lancet Resp Med. 1(6):462–470. (IF=Not available)
  • Hanekom M, Streicher EM, van de Berg D, Cox H, McDermid C, Bosman M, Gey van Pittius NC, Victor TC, Kidd M, van Soolingen D, van Helden PD, Warren RM. (2013) Population Structure of Mixed Mycobacterium tuberculosis Infection Is Strain Genotype and Culture Medium Dependent. Plos One. 8(7):e70178. (IF=3.73)
  • Jacobson KR, Theron D, Kendall EA, Franke MF, Barnard M, van Helden PD, Victor TC, Streicher EM, Murray MB, Warren RM. (2013) Implementation of GenoType MTBDRplus Reduces Time to Multidrug-Resistant Tuberculosis Therapy Initiation in South Africa. Clin Infect Dis. 56(4):503-508. (IF=9.37)
  • Katale BZ, Mbugi EV, Karimuribo ED, Keyyu JD, Kendal S, Kibiki GS, Godfrey-Faussett P, Michel AL, Kazwala RR, van Helden PD, Matee MI. (2013) Prevalence and risk factors for infection of bovine tuberculosis in indigenous cattle in the Serengeti ecosystem, Tanzania. BMC Vet Res. 9:267. (IF=1.86)
  • Kayigire XA, Friedrich SO, Venter A, Dawson R, Cillespie SH, Boeree MJ, Heinrich N, Hoelscher M, Diacon AH. (2013) Direct Comparison of Xpert MTB/RIF Assay with Liquid and Solid Mycobacterial Culture for Quantification of Early Bactericidal Activity. J. Clin. Microbiol. 51(6):1894-1898. (IF=4.07)
  • Kleinsteuber K, Heesch K, Schattling S, Kohns M, Sander-Jülch C, Walzl G, Hesseling A, Mayatepek E, Fleischer B, Marx FM, Jacobsen M. (2013) Decreased Expression of miR-21, miR-26a, miR-29a, and miR-142-3p in CD4(+) T Cells and Peripheral Blood from Tuberculosis Patients. PLoS One. 2013; 8(4):e61609. (IF=3.73)
  • Kleynhans L, Du Plessis N, Allie N, Jacobs M, Kidd M, van Helden PD, Walzl G, Ronacher K. (2013) The Contraceptive Depo Medroxyprogesterone Acetate Impairs Mycobacterial Control and Inhibits Cytokine Secretion in Mice Infected with M. tuberculosis. Infection and Immunity. 81(4):1234-1244. (IF=4.07)
  • Klopper M, Warren RM, Hayes C, Gey van Pittius NC, Streicher EM, Muller B, Sirgel FA, Chabula-Nxiweni M, Hoosain E, Coetzee G, van Helden PD, Victor TC, Trollop AP. (2013) Emergence and spread of Extensively and Totally Drug Resistant Tuberculosis in South Africa. EID. 19(3):439-445. (IF=5.99)
  • Kolwijck E, Mitchell M, Venter A, Friedrich SO, Dawson R, Diacon AH. (2013) Short-term storage does not affect the quantitative yield of Mycobacterium tuberculosis in sputum in early bactericidal activity studies. J. Clin. Microbiol. 51(4):1094-1098. (IF=4.07)
  • Le Roex N, Koets AD, van Helden PD, Hoal EG. (2013) Gene Polymorphisms in African Buffalo Associated with Susceptibility to Bovine Tuberculosis Infection. PloS One. 8(5):e64494. (IF=3.73)
  • Le Roex N, van Helden PD, Koets AP, Hoal EG. (2013) Bovine TB in livestock and wildlife: what's in the genes? Physiol Genomics. 45(15):631-637. (IF=2.81)
  • Loots DT, Meissner-Roloff RJ, Newton-Foot M, Gey van Pittius NC. (2013) A metabolomics approach exploring the function of the ESX-3 type VII secretion system of M. smegmatis. Metabolomics. 9:631–641. (IF=4.43)
  • Marais B, Mlambo C, Rastogi N, Zozio T, Duse A, Victor TC, Marais E, Warren RM.  (2013) Epidemic spread of multidrug-resistant (MDR) tuberculosis in Johannesburg, South Africa. J. Clin. Microbiol. 51(6):1818-1825. (IF=4.07)
  • Mihret A, Bekele Y, Bobosha K, Kidd M, Aseffa A, Howe R, Walzl G. (2013) Plasma cytokines and chemokines differentiate between active disease and non-active tuberculosis infection. J Infect. 66:357-365. (IF=4.07)
  • Müller B, Chihota VN, Pillay M, Klopper M, Streicher EM, Coetzee G, Trollip A, Hayes C, Bosman ME, Gey van Pittius NC, Victor TC, Gagneux S, van Helden PD, Warren RM. (2013) Programmatically selected multidrug-resistant strains drive the emergence of extensively drug-resistant tuberculosis in South Africa. Plos One.  8(8):e70919. (IF=3.73)
  • Muller B, Durr S, Alonso S, Hattenhoff J, Laisse CJM, Parsons SDC, van Helden PD, Zinsstag J. (2013) Zoonotic Mycobacterium bovis - induced tuberculosis in humans. EID. 19(6):899-908. (IF=5.99)
  • Newton-Foot M, Gey van Pittius NC. (2013) The complex architecture of mycobacterial promoters. Tuberculosis. 93:60-74. (IF=3.03)
  • Parsons SDC, Drewe JA, Gey van Pittius NC, Warren RM, van Helden PD. (2013) Novel Cause of Tuberculosis in Meerkats, South Africa. EID. 19(12):2004-2007. (IF=5.99)
  • Phalane K, Kriel M, Loxton A, Menezes A, Stanley K, van der Spuy G, Walzl G, Chegou NN. (2013) Differential expression of host biomarkers in saliva and serum samples from individuals with suspected pulmonary tuberculosis. Mediators of Inflammation. Article ID 981984. (IF=3.88)
  • Sao Emani C, Williams M, Wiid I, Hiten N, Viljoen A, Pietersen R, van Helden PD, Baker B. (2013) Ergothioneine is a secreted antioxidant in Mycobacterium smegmatis. Antimicrobial Agents and Chemotherapy. 57(7):3202-3207. (IF=4.57)
  • Shanley CA, Streicher EM, Warren RM, Victor TC, Orme IM. (2013) Characterization of W-Beijing isolates of Mycobacterium tuberculosis from the Western Cape. Vaccine. 31(50):5934-5939. (IF=3.49)
  • Shenai S, Amisano D, Ronacher K, Kriel M, Banada P, Song T, Lee M, Joh J, Winter J, Thayer R, Via L, Kim S, Barry C, Walzl G, Alland D. (2013) Exploring alternative biomaterials for diagnosis of pulmonary tuberculosis in HIV negative patients using the Xpert MTB/RIF assay. J Clin Microbiol. 51(12):4161-4166. (IF=4.07)
  • Sirgel FA, Warren RM, Bottger EC, Klopper M, Victor TC, van Helden PD. (2013) The rationale for using Rifabutin in the treatment of MDR and XDR tuberculosis outbreaks. Plos One. 8(3):e59414. (IF=3.73)
  • Sutherland J, Lalor M, Black GF, Ambrose L, Loxton A, Chegou N, Kassa  D, Mihret A, Howe R, Mayanja-Kizza H, Gomez M, Donkor S, Franken K, Boom W, Thiel B, Crampin A, Hanekom W, Klein M, Parida S, Ota M, Walzl G, Ottenhoff T, Dockrell H, Kaufmann S and the GCGH Biomarkers for TB consortium. (2013)  Analysis of host responses to secreted, latent and reactivation Mycobacterium tuberculosis antigens in a large multi-site study of subjects with different TB and HIV infection states in sub-Saharan Africa. PLoS One. 8(9):e74080. (IF=3.73)
  • Sutherland JS, Loxton AG, Haks MC, Kassa D, Ambrose L, Lee JS, Ran L, van Baarle D, Maertzdorf J, Howe R, Mayanja-Kizza H, Boom WH, Thiel BA, Crampin AC, Hanekom W, Ota MO, Dockrell H, Walzl G, Kaufmann SH, Ottenhoff TH; the GCGH Biomarkers for TB consortium. Differential gene expression of activating Fcγ receptor classifies active tuberculosis regardless of human immunodeficiency virus status or ethnicity. Clin Microbiol Infect. 2013 Sep 5. doi: 10.1111/1469-0691.12383. PMID: 24205913. (IF=4.58)
  • Viegas S, Machado A, Groenheit R, Ghebremichel S, Pennhag A, Gudo P, Cuna Z, Langa E, Miotto P, Cirillo D, Rastogi N, Warren RM, van Helden PD, Koivula T, Kallenius G. (2013) Mycobacterium tuberculosis Beijing Genotype Is Associated with HIV Infection in Mozambique. Plos One. 8(8):e71999. (IF=3.73)
  • Viljoen AJ, Kirsten CJ, Baker B, van Helden PD, Wiid IJ. (2013) The Role of Glutamine Oxoglutarate Aminotransferase and Glutamate Dehydrogenase in Nitrogen Metabolism in Mycobacterium bovis BCG PLoS One 8(12):e84452. (IF=3.73)

Non Peer-Reviewed Articles (Total: 4)

  • Van Helden PD. (2013) Data-driven hypothesis. EMBO reports. 14:104. (IF=7.19)
  • Van Helden PD, Hoal EG. (2013) A New TB Vaccine: fact or fiction? CIMID. 36(3): 287-294. (IF=1.81)
  • Van Helden PD, van Helden LS, Hoal EG. (2013) One world, one health. EMBO reports. 14(6):497-501. (IF=7.19)
  • Kana BD, Churchyard G. (2013) Tuberculosis: The global killer. South African Journal of Science, 109:9-10. (IF=0.84)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 90)

Plenary/Keynote Lectures

  • Kana BD. Developing a Scientific Career. Invited Plenary lecture Young Researchers Forum – South Africa Society for Human Genetics. School of Public Health, Wits University,South Africa, 5-9 October 2013
  • Kana BD. Stretching the bacterial skin: How much do we know about mycobacterial growth? Invited Opening Plenary lecture presented at the Gordon Research Seminar on Tuberculosis Drug Development. Il Ciocco Hotel and Resort, Lucca (Barga), Italy, 21 – 22 July 2013
  • Gopinath K, Venclovas C, Ioerger T, Sacchettini JC, McKinney JD, Mizrahi V. Vitamin B12 metabolism in M. tuberculosis. Plenary lecture delivered at the Keystone Symposium on TB: Understanding the Enemy, Whistler, B.C., Canada, 13-18 March, 2013
  • Mizrahi V. Understanding and targeting core metabolic pathways in M. tuberculosis. Inaugural Lecture, Centre for Structural Systems Biology Seminar Series, EMBL, Hamburg, Germany, 18 June 2013.
  • Mizrahi V. Understanding the biology of Mycobacterium tuberculosis and why it matters. Keynote Lecture, Annual Academic Day, Faculty of Health Sciences, Stellenbosch University, 15 August 2013.
  • Mizrahi V. Tuberculosis drug discovery: lessons learned and future prospects. Plenary lecture, SACORE TB Symposium, Cape Town, 31 August 2013.
  • Mizrahi V. Gearing for the future: configuration, culture and values for research and training. Keynote Lecture, 2013 UCT Research Function, University of Cape Town, 10 September 2013.

Invited Talks

  • Ealand CS, Kana BD. The Role of Mycobacterial DD-Carboxypeptidases in Peptidoglycan Remodeling and Turnover. Short Talk and Poster Presentation, Tuberculosis: Understanding the Enemy Keystone Conference. March 13 - March 18, 2013, Whistler Conference Centre, Whistler, British Columbia Canada
  • Gordhan BG. Empowering women to be the best researcher/scientific writers. Oral presentation at the "Women in Science and technology conference" 14-15th, Sandton Convention Centre, Johannesburg.
  • Kana BD. Differential Bacterial Culturability in tuberculous sputum: Can we look at both sides of the same coin at once? Invited talk presented at the Gordon Research Conference on Tuberculosis Drug Development. Il Ciocco Hotel and Resort, Lucca (Barga), Italy, 22 – 26 July 2013
  • Kana BD. What makes a good target? Invited talk presented at the Working group on new TB drugs Symposium at the Gordon Research Conference on Tuberculosis Drug Development. Il Ciocco Hotel and Resort, Lucca (Barga), Italy, 22 – 26 July 2013
  • Kana BD. Eliminating TB. Lecture Given at the 8th Prestigious Research Lecture in the Faculty of Health Sciences. Wits School of Public Health. 29th May 2013
  • Kana BD. Peptidoglycan Remodelling during TB Infection: Separation Anxiety and Bipolar Disorder in mycobacterial cells. Invited lecture as part of the Frontiers in Biological Sciences Seminar Series, Tsinghua University, Beijing, China. 12 November 2013
  • Kana BD. Success in Academic Careers. Invited Lecture and workshop. Tsinghua University, Beijing, China. 11 November 2013
  • Kana BD. Counter-screening models for TB drug development – Exploiting the mycobacterial cell wall, 30th October 2013, Helmholtz Institute-South Africa joint research meeting, Braunschweig, Germany.
  • Kana BD. Peptidoglycan Remodelling: Implications for mycobacterial growth and TB disease. Invited Lecture, 16 September 2013, Johns Hopkins University, Baltimore, USA.
  • Ditse Z, Mizrahi V, Warner DF. Replication fidelity in M. tuberculosis. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013.
  • Ditse Z, Mizrahi V, Warner DF. Replication fidelity in the microevolution of Mycobacterium tuberculosis. 5th CLS/HUB Postgraduate Research Day, UCT, 4 September 2013. [Winner, best oral presentation prize]
  • Ditse Z, Mizrahi, V, Warner DF. Replication fidelity in the microevolution of Mycobacterium tuberculosis. FIDSSA Conference, Drakensberg, 10-12 [Winner, best oral presentation prize]
  • Ditse Z. Replication fidelity in the microevolution of mycobacteria. IDM/MCB Carnegie Scholars Retreat, Silver Mist Mountain Lodge, Cape Town, 14 October 2013.
  • Evans J, Mizrahi V. Identifying vulnerable steps in CoA biosynthesis. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Evans J, Mizrahi V. CoaBC, CoaD and CoaE. Lecture presented at the Second HIT-TB Consortium meeting, Cape Town, 26-28 February 2013
  • Mizrahi V. Conditional mutants as tools for TB drug discovery. Invited lecture, SA MRC-HZI Symposium on Infection Research, Braunschweig, Germany, 30 October 2013.
  • Mizrahi V. Target-based whole-cell screening. Lecture presented at the Second HIT-TB Consortium meeting, Cape Town, 26-28 February 2013
  • Mizrahi V. Target-based whole-cell screening. Talk presented at NITD & H3-D workshop, Vineyard Hotel, Cape Town, 12 June 2013
  • Mizrahi V. Co-factor metabolism in M. tuberculosis: doing things differently. Invited talk presented at the MRC Centre for Molecular Bacteriology & Infection, Imperial College, London, 17 June 2013.
  • Mizrahi V. Tuberculosis drug discovery research: a personal perspective from South Africa. After-dinner speech presented at the DST budget vote dinner, South African Museum, Cape Town, 16 May 2013.
  • Mizrahi V. Tuberculosis drug discovery: lessons learned and future prospects. SACORE TB Symposium, Cape Town, 31 August 2013.
  • Moosa A, Seldon R, Mizrahi V, Warner DF. Smart screening for TB drug discovery. H3-D TB Projects meeting, Hermanus, 21-22 November, 2013.
  • Narrandes N, Mizrahi V, Kana BD. Functional characterization of molybdopterin synthase-encoding genes in mycobacteria. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Singh V, Evans J, Warner D, Mizrahi V. The role of the thymidylate synthase, ThyX, in the mechanism of action of 5-fluorouracil in M. tuberculosis. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Singh V, Evans J, Warner DF, Mizrahi V. Update on conditional knockdown of Wag31. Consortium Meeting 6, MM4TB, Lille, France, 4-5 July 2013.
  • Warner DF. How M. tuberculosis gets its vitamin B12 shot. Invited talk presented at the University of Oslo, Norway, 2 April 2013
  • Warner DF. SATRII Biology Component capacity. Lecture presented at the Second HIT-TB Consortium meeting, Cape Town, 26-28 February 2013
  • Warner DF. The changing nature of the role of the institution and challenges for supervisors with regards to research students. Invited talk presented at the UFCT Postdoctoral Supervision Training Programme, Mont Fleur, Stellenbosch, 11-12 November 2013
  • Warner DF. Translesion synthesis in Mycobacterium tuberculosis and its impact on antibiotic resistance. 11th International Conference on Environmental Mutagens (11th ICEM), the XI Congress of SBMCTA and the IX Congress of ALAMCTA: Bacterial Models for Mutagenesis Studies: From Basic Mechanisms to Infectious Diseases, Foz do Iguaçu Brazil, 2-8 November 2013.
  • Warner DF. What it means to be an academic in the 21st century. Launch of Phase II of the Carnegie Corporation’s Developing the Next Generation Academics programme, UCT, 23 April 2013
  • Sao Emani C. The role of ergothioneine in mycobacteria. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Macingwana L. An Old drug: Sulfamethoxazole. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Anderson D. TB Genomics. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Fortuin S. TB Proteomics. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Newton-Foot M. TB Functional Biology. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • De Vos M. TB Diagnostics. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Klopper M. Molecular epidemiology of Drug resistant TB. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Grobbelaar M. Rifampicin resistance. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Black P. Novel mechanisms of resistance. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Moller M. Host TB genetics. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Du Plessis N. Innate immunity to mycobacterial infections. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Kleynhans L. Immune endocrine interactions during TB. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Chegou NN. Host Biomarkers for active TB. Talk presented at the CBTBR Symposium, Stellenbosch University, 9-10 April 2013
  • Chegou NN. Evaluation of M. tuberculosis specific host cytokine signatures in whole blood culture supernatants as diagnostic markers for active tuberculosis. Talk presented at the 2nd South African-German workshop on IT-based Technologies for Rural Health Care. Berlin University of Applied Sciences, Berlin, Germany. 25-28 February 2013
  • Chegou NN. Utility of M. tuberculosis specific host cytokine signatures in whole blood culture supernatants as diagnostic markers tuberculosis disease. Talk presented at the MRC Early Career Scientist Convention. MRC, Parow, Cape Town, South Africa. 16 October 2013
  • Daya M. A panel of ancestry informative markers for the South African Coloured population. Talk presented at the DAAD conference. Stellenbosch Protea Hotel, Stellenbosch, South Africa. 08-10 November 2013
  • De Vos M, Louw GE, van Helden PD, Victor TC, Warren RM. Understanding the biology of poly-rifampicin resistance in Mycobacterium tuberculosis. Talk presented at the 34th European Society of Mycobacteriology Annual Congress. Demidoff Hotel, Florence, Italy. 30 June - 03 July 2013
  • Du Plessis N. MDSCs in TB. Talk presented at the Respiratory Unit Research Meeting. Tygerberg Pulmonary Division, Cape Town, South Africa. 16 October 2013
  • Du Plessis N. Suppressive Innate Immunity to Mycobacterium tuberculosis infection. Talk presented at the Institute of Infectious Diseases Seminars. University of Cape Town, 30 October 2013
  • Essone P. Diagnostic utility of antigen-stimulated host markers in an overnight whole blood culture assay. Talk presented at the EDCTP. Dakar Hotel, Dakar, Senegal. 20 October - 20 October 2013
  • Fortuin S. Deciphering the impact of the evolution of drug resistance on the physiology of Mycobacterium tuberculosis. Talk presented at the Southern African Consortuim for Research Excellence TB Workshop. Vineyard Hotel, Cape Town, South Africa. 31 August - 02 September 2013
  • Fortuin S. Proteomic analysis of Mycobacterium tuberculosis strains demonstrating varying levels of virulence and drug resistance. Talk presented at the Southern African Corsortium for Research Excellence TB Meeting. Vinyard Hotel, Cape Town, South Africa. 31 August - 02 September 2013
  • Hoal EG. Life in South Africa: Political situation, recent history, social life. Talk presented at the Christian Albrechts University Strategic Planning workshop. Christian Albrechts University, Kiel, Germany. 14-17 November 2013
  • Klopper M, Streicher EM, Warren RM, Victor TC. The evolution of Totally Drug-Resistant TB in South Africa. Talk presented at the Southern African Consortuim for Research Excellence TB Meeting. The Vineyard Hotel, Cape Town, South Africa. 31 August - 04 September 2013
  • Le Roex N, van Helden PD, Hoal EG, Parsons S, Goosen W Bovine TB in buffalo: what's in the genes?. Talk presented at the Annual Bovine Tuberculosis Meeting and Workshop. Veterinary Wildlife Services, Kruger National Park, South Africa. 12-13 September 2013
  • Malherbe S, Walzl G. Recent, current and future biomarker discovery at SUN-IRG to aid TB diagnosis and cure. Talk presented at the Desmond Tutu Trial Centre Dissemination meeting. Klein Joostenberg, Cape Town, South Africa. 13 December 2013
  • Moller M. TB research in South Africa. Talk presented at the Christian Albrechts University Strategic Planning workshop. Christian Albrechts University, Kiel, Germany. 14-17 November 2013
  • Ronacher-Mansvelt K. Biomarkers of TB treatment response. Talk presented at the Meeting. Helmholz Insitute, Braunschweig, Germany. 28 October 2013
  • Sampson SL. Mice in TB Research - what's new and how is this useful in a Southern African context? Talk presented at the Southern African Consortium for Research Excellence, Workshop: Understanding and intervening in tuberculosis in Southern Africa. Vineyard Hotel, Cape Town, South Africa. 31 August 2013
  • Steyn NL, Newton-Foot M, Warren RM, van Helden PD, Gey van Pittius NC. Investigating the localisation of the ESX-3 secretion system in Mycobacterium smegmatis. Talk presented at the 57th FHSM Annual Academic Day. Stellenbosch University, Cape Town, South Africa. 14-15 August 2013
  • Van Helden PD. Internationalisation in Science. Talk presented at the Christian Albrechts University Strategic Planning workshop. Christian Albrechts University, Kiel, Germany. 14-17 November 2013
  • Van Helden PD. Rapid genetic detection of pyrazinamide susceptibility. Talk presented at the The Annual ACTG Network Meeting. Grand Hyatt Washington Hotel, Washington DC, USA. 29 July - 02 August 2013
  • Van Helden PD. Session chair. Talk presented at the Gordon Research Conference on Tuberculosis Drug Development. Renaissance Tuscany Il Ciocco Resort, Lucca (Barga), Italy. 21-26 July 2013
  • Walzl G. TB biomarkers in peripheral blood. Talk presented at the 34th Annual Conference of the European Society of Mycobacteriology. Florence, Italy. 30 June - 03 July 2013
  • Walzl G. TB biomarkers in peripheral blood. Talk presented at the Biomarkers for Tuberculosis: New Questions, New Tools Meeting.  Washington, USA. 8-11 September 2013
  • Warren RM. Rapid genetic detection of pyrazinamide susceptibility. Talk presented at the The Critical Path to TB Drug Regimens Workshop. Marriott Renaissance Hotel, Washington, USA. 30 September - 01 October 2013
  • Warren RM. The Genesis and Transmission of Drug Resistant TB in SA: Molecular Epidemiologic View. Talk presented at the XDR-TB Workshop. University of Cape Town, South Africa. 16-17 November 2013
  • Warren RM. The Molecular Epidemiology of Drug-Resistant TB in South Africa. Talk presented at the Southern African Consortuim for Research Excellence TB Workshop. Vineyard Hotel, Cape Town, South Africa. 31 August - 02 September 2013
  • Warren RM. Virtual DNA sequencing: Lights-on/Lights-off assay platform rpoB as an example. Talk presented at the The Critical Path to TB Drug Regimens Workshop. Marriott Renaissance Hotel, Washington, USA. 30 September - 01 October 2013
  • Warren RM. The Molecular Epidemiology of Drug-Resistant TB in SA. Talk presented at the 3rd Pan-African Infectious Diseases Conference. Gallagher Convention Centre, Johannesburg, 07-08 May 2013
  • Williams MJ, Peixoto B, Manca C, Kaplan G, Mizrahi V, Kana BD. Biosynthesis of bis-molybdopterin guanine dinucleotide is dispensable for virulence of Mycobacterium tuberculosis. Talk presented at the 57th FHSM Annual Academic Day. Stellenbosch University, Cape Town, South Africa. 14-15 August 2013

Posters

  • Kana BD. Resuscitation promoting factors in bacterial growth and cell wall remodelling - Extreme Makeover for the Cell wall. Poster presentation at the Annual HHMI Scholars Meeting, HHMI Janelia Farm Research Campus, Ashburn, Virginia, USA. 10-12 February 2013
  • Kana BD. A role for N-acetylmuramoyl-L-alanine amidases and DD-carboxypeptidases in mycobacterial growth and cell division. Poster presentation at the Bacterial Cell Biology Conference 2013, Occidental Grand Xcaret Resort, Mexico. 9-12 December 2013
  • Singh V, Evans J, Warner D, Mizrahi V. The role of the thymidylate synthase, ThyX, in the mechanism of action of 5-fluorouracil in M. tuberculosis. Poster presented at the Keystone Symposium on TB: Understanding the Enemy, Whistler, Vancouver, Canada, 13-18 March 2013.
  • Evans J, Abrahams GL, Mizrahi V. Identification of vulnerable steps in the coenzyme A biosynthesis pathway of Mycobacterium tuberculosis. Poster presented at the Gordon Research Conference on Tuberculosis Drug Development, Lucca (Barga), Italy, 22-26 July 2013
  • Naran K, Mizrahi V, Warner DF. Mechanisms of antibiotic subversion in M. tuberculosis: role of the SOS response. Poster presented at the Gordon Research Conference on Tuberculosis Drug Development, Lucca (Barga), Italy, 22-26 July 2013
  • Mjambili F, Ngoroje M, Naran K, Warner DF, Chibale K. Synthesis, ADME and pharmacological evaluation of 2-amino-4-(2--‐pyridyl)-thiazole derivatives as antimycobacterial agents. Poster presented at the Gordon Research Conference on Tuberculosis Drug Development, Lucca (Barga), Italy, 22-26 July 2013
  • Soares De Melo C, Feng T, Gessner R, Boshoff H, Moosa A, Naran K, Warner DF, Street L,  Chibale K. Hit-to-lead progression of anti-tuberculosis leads. Poster presented at the Gordon Research Conference on Tuberculosis Drug Development, Lucca (Barga), Italy, 22-26 July 2013
  • Hammond-Aryee K. Toxoplasama gondii seroprevalence studies in Africa. Poster presented at the 12th International Conference on Toxoplasmosis. St Cathrine's College, Oxford, UK. 22-27 June 2013
  • Hammond-Aryee K. Toxoplasama gondii seroprevalence studies in Africa. Poster presented at the EMBO Aids related Mycoses conference 2013. University of Cape Town, Cape Town, South Africa. 03-05 July 2013
  • Klopper M. Deciphering the evolution of totally drug-resistant tuberculosis - a catalogue of genome variation at single-base pair resolution. Poster presented at the Keystone Symposia. Tuberculosis: Understanding the Enemy. Whistler Conference Centre, Whistler, Canada. 13-18 March 2013
  • Dippenaar A, Warren RM, Gey van Pittius NC, van Helden PD, McNerney R, Clark T, Abdallah AM, Pain A Deciphering the evolutionary history of the Mycobacterium tuberculosis Latin American Mediterranean (LAM) genotype. Poster presented at the 34th European Society of Mycobacteriology Annual Congress. Hotel Demidoff, Florence, Italy. 30 June - 03 July 2013
  • Sao Emani, Williams MJ, Wiid IJF, Baker B. Ergothioneine is secreted by mycobacteria. Poster presented at the 57th FHSM Annual Academic Day. Stellenbosch University, Tygerberg Campus, Cape Town, South Africa. 14-15 August 2013
  • Gallant JL, Viljoen AJ, Tshoko S, van Helden PD, Wiid IJF. Purification and characterisation of the aspartate aminotransferases from the mycobacteria. Poster presented at the 57th FHSM Annual Academic Day. Stellenbosch University, Tygerberg Campus, Cape Town, South Africa. 14-15 August 2013
  • Chegou NN, Walzl G, Loxton A, Ronacher-Mansvelt K, Malherbe S, Smith B. Utility of M.tuberculosis specific host cytokine signatures in whole blood culture supernatants for the diagnosis of TB disease. Poster presented at the Biomarkers for Tuberculosis, New Questions, New Tools and FDA Workshop on Immune Correlates of Protection against Tuberculosis Vaccines. Westfields Marriott, Chantilly, Virginia, USA. 08-11 September 2013
  • Chegou NN, Walzl G, Loxton A, Ronacher-Mansvelt K, Smith B. Beyond the IFN-gamma horizon: Biomarkers for immunodiagnosis of infection with M. Tuberculosis. Poster presented at the Biomarkers for Tuberculosis: New Questions, New Tools and FDA Workshop on Immune Correlates of Protection against Tuberculosis Vaccines. Westfields Marriott, Chantilly, Virginia, USA. 08-11 September 2013
  • Newton-Foot M, Warren RM, van Helden PD, Gey van Pittius NC. The evolution of the mycobacterial Type VII ESX secretion system. Poster presented at the 57th FHSM Annual Academic Day. Stellenbosch University, Cape Town, South Africa. 14-15 August 2014
  • Steyn N, Newton-Foot M, Warren R, van Helden PD, Gey van Pittius NC. Investigating the localisation of the ESX-3 secretion system in Mycobacterium smegmatis. Poster presented at the Keystone Symposium: Tuberculosis Understanding the enemy. Whistler Conference Centre, Vancouver, Canada. 13-18 March 2013
  • Whitfield M, Streicher E, York T, Mardarowicz I, Scott L, Stevens W, Van Helden PD, Van Rie A, Warren RM. Association between Genotypic and Phenotypic Pyrazinamide Resistance in Rifampicin Resistant Mycobacterium tuberculosis Isolates. Poster presented at the 57th Annual Academic Day. Stellenbosch University, Tygerberg, South Africa. 14-15 August 2013
  • Klopper M, Hill-Cawthorne G, Abdallah AM, Rangkuti F, Hayes C, McNerney R, Clarke T, Dheda K, Trollip A, van Helden PD, Victor TC, Warren RM, Pain A. Deciphering the evolution of totally drug-resistant TB. Poster presented at the Keystone symposia: Tuberculosis: Understanding the enemy (x8). Whistler Conference Centre, Vancouver, Canada. 13-18 March 2013
  • Chegou NN, Ronacher-Mansvelt K, Loxton A, Walzl G. Utility of host markers detected in Quantiferon supernatants for the diagnosis of tuberculosis in children in a high-burden setting. Poster presented at the Keystone Symposium: Tuberculosis: Understanding the Enemy and Host Response in Tuberculosis. Whistler Conference Centre, Vancouver, Canada. 13-18 March 2013
  • Chegou NN, Walzl G. Utility of M.tuberculosis specific host cytokine signatures in whole blood culture supernatants for the diagnosis of TB disease. Poster presented at the Biomarkers for Tuberculosis, New Questions, New Tools. Washington Dulles, Chantilly, Virginia, USA. 08-11 September 2013
  • Chegou NN, Walzl G. Beyond the IFN‐g horizon: Biomarkers for immunodiagnosis of infection with M. tuberculosis. Poster presented at the Biomarkers for Tuberculosis, New Questions, New Tools. Washington Dulles, Chantilly, Virginia, USA. 08-11 September 2013
  • Loxton AG. Immunogenicity of novel DosR candidate vaccine antigens of M.tb in ARV-naive HIV+ patients with a range of CD4 counts from a high-burden country (South Africa). Poster presented at the AIDS Vaccine 2013. Barcelona, Barcelona, Spain. 07-10 October 2013
  • Ronacher-Mansvelt K, Kleynhans L, Ruzive S, Ehlers L, Thiart L, Walzl G. Hormones as markers of TB treatment response. Poster presented at the Keystone Meeting. Whistler Conference Centre, Vancouver, Canada. 13-18 March 2013
2012

* The Names in bold are CBTBR staff

Books / Chapters in Books (Total: 2)

  • De Wit E, Moller M, Hoal EG. (2012) Genetic Perspectives of Tuberculosis in Southern Africa. In: Genomics and Health in the Developing World, D Kumar (Ed) Oxford University Press, New York
  • Warner DF, Mizrahi V. (2012) Tuberculosis Drug Discovery: Target Identification and Validation. In: Drug Discovery in Africa (Chibale, K.C., Davies-Coleman, M. & Masimerembwa, C., eds.), Springer, ch. 3, pp. 53-84

Articles in Peer-Reviewed Journals (Total: 47)

  • Griffin JE, Pandey AK, Gilmore SA, Mizrahi V, McKinney JD, Bertozzi CR, Sassetti CM. (2012) Cholesterol catabolism by Mycobacterium tuberculosis requires transcriptional and metabolic adaptation. Chem Biol. 19(2): 218-227. (IF=5.976)
  • Warner DF, Mizrahi V. (2012) A pseudokinase debut at the mycobacterial cell wall. Sci Signal. 5(208):pe3. (IF=7.500)
  • Warner DF, Mizrahi V. (2012) Approaches to target identification and validation for tuberculosis drug discovery: a UCT perspective. S Afr Med J. 102(6): 457-460. (IF=1.610)
  • Abrahams GL, Kumar A, Savvi S, Hung AW, Wen S, Abell C, Barry CE III, Sherman DR, Boshoff HIM, Mizrahi V. (2012) Pathway-selective sensitization of Mycobacterium tuberculosis for target-based whole-cell screening, Chem Biol. 19:844-854. (IF=5.976)
  • Barnard M, Gey van Pittius NC, van Helden PD, Bosman M, Coetzee G, Warren RM. (2012) Diagnostic performance of Genotype® MTBDRplus Version 2 line probe assay is equivalent to the Xpert®MTB/RIF assay. J Clin. Microbiol. 50(11):3712-3716. (IF=4.258)
  • Chegou NN, Black GF, Loxton AG, Stanley K, Essone PN, Klein MR, Parida SK, Kaufmann SH, Doherty TM, Friggen AH, Franken KL, Ottenhoff TH, Wazlz G. (2012)  Potential of novel Mycobacterium tuberculosis infection phase-dependent antigens in the diagnosis of TB disease in a high burden setting. BMC Infect Dis. 12(1):10. (IF=3.011)
  • Chegou NN, Essone PN, Loxton AG, Stanley K, Black GF, van der Spuy GD, van Helden PD, Franken KL, Parida SK, Klein MR, Kaufmann SHE, Ottenhoff TH, Walzl G. (2012) Potential of Host Markers Produced by Infection Phase-dependent Antigen-stimulated Cells for the Diagnosis of Tuberculosis in a Highly Endemic Area. PLoS ONE. 7(6):e38501. (IF=4.610)
  • Chihota V, Müller B, Mlambo C, Pillay M, Tait M, Streicher EM, Marais E, Van der Spuy G, Hanekom M, Coetzee G, Trollip A, Hayes C, Bosman M, Gey van Pittius NC, Victor TC, Van Helden PD, Warren RM. (2012) The population structure of multi-and extensively drug-resistant tuberculosis in South Africa. J Clin Microbiol. 50(3): 995-1002. (IF=4.258)
  • Cohen T, van Helden PD, Wilson D, Colijn C, McLaughlin MM, Abubakar I, Warren RM. (2012) Mixed strain Mycobacterium tuberculosis infections: implications for tuberculosis treatment and control. Clin Microbiol Rev. 25(4):708-719. (IF=19.832)
  • De Beer JL, Kremer K, Ködmön C, Supply P, van Soolingen D,Global network for the molecular surveillance of tuberculosis 2009 (Consortium of authors include Warren RM (2012) First worldwide proficiency study on variable numbers of tandem repeats typing of Mycobacterium tuberculosis complex strains. J Clin Microbiol. 50(3): 662-669. (IF=4.258)
  • Diacon AH, Maritz S, Venter A, Van Helden PD, Dawson R, Donald P. (2012) Time to liquid culture positivity as a substitute for colony counting on agar plates in early bactericidal activity studies of antituberculosis agents. Clin. Microbiol Infect 18(7): 711-717. (IF=4.034)
  • Diacon AH, Dawson R, du Bois J, Narunsky K, Venter A, Donald PR, van Niekerk C, Erondu N, Ginsberg AM, Becker P, Spigelman MK. (2012) Phase II Dose-Ranging Trial of the Early Bactericidal Activity of PA-824. Antimicrob Agents Chemother. 56(6):3027-3031. (IF=4.680)
  • Diacon AH, Donald PR, Pym A, Grobusch M, Patientia RF, Mahanyele R, Bantubani N, Narasimooloo R, De Marez T, van Heeswijk R, Lounis N, Meyvisch P, Andries K, McNeeley DF. (2012) Randomized pilot trial of eight weeks of bedaquiline (TMC207) treatment for multidrug-resistant tuberculosis: long-term outcome, tolerability, and effect on emergence of drug resistance. Antimicrob Agents Chemother. 56(6): 3271-3276. (IF=4.680)
  • Donald PR, Maritz JS, Diacon AH. (2012) Pyrazinamide pharmacokinetics and efficacy in adults and children. Tuberculosis (Edinb) 92(1): 1-8. (IF=3.149)
  • Drewe JA, O’Riain MJ, Beamish E, Currie H, Parsons S. (2012) Survey of infections transmissible between baboons and humans, Cape Town, South Africa. Emerg Infect Dis 18(2): 298-301. (IF=6.996)
  • Gey van Pittius NC, van Helden PD, Warren RM. (2012) Characterization of Mycobacterium orygis. Letter in Emerg Infect Dis. 18(10):1708-1709. (IF=6.689)
  • Hatherill M, Verver S, Mahomed H; Taskforce on Clinical Research Issues, Stop TB Partnership Working Group on TB Vaccines (Walzl, G). (2012) Consensus statement on diagnostic end points or infant tuberculosis vaccine trials. Clin Infect Dis. 54(4): 493-501. (IF=7.898)
  • Hesseling AC, Kim S, Madhi S, Nachman S, Schaaf HS, Violari A, Victor TC, McSherry G, Mithell C, Cotton MF. (2012) High prevalence of drug resistance amongst HIV-exposed and –infected children in a tuberculosis prevention trial. Int J Tuberc Lung Dis 16(2): 192-195. (IF=2.426)
  • Katale BZ, Mbugi EV, Kendal S, Fyumagwa RD, Kibiki GS, Godfrey-Faussett P, Keyyu JD, van Helden PD, Matee MI. (2012) Bovine tuberculosis at the human-livestock-wildlife interface: Is it a public health problem in Tanzania? A review. Onderstepoort Journal of Veterinary Research. 79(2):1-8. (IF=0.890)
  • Kiser JJ, Zhu R, D’argenio DZ, Cotton MF, Bobat R, McSherry GD, Madhi SA, Carey VJ, Seifart HI, Werely CJ, Fletcher CV. (2012) Isoniazid Pharmacokinetics, Pharmacodynamics, and dosing in South African infants. Ther Drug Monit. 34(4):446-451. (IF=2.605)
  • Loxton AG, Black GF, Stanley K, Walzl G. (2012) Heparin-Binding Hemagglutinin Induces IFN-γ+ IL-2+ IL-17+ Multifunctional CD4+ T Cells during Latent but Not Active Tuberculosis Disease. Clin Vaccine Immunol. 19(5): 746-751. (IF=2.567)
  • Macingwana L, Baker B, Ngwane A, Harper C, Cotton M, Hesseling A,  Diacon A, van Helden PD, Wiid I. (2012) Sulfamethoxazole enhances the antimycobacterial activity of Rifampicin. J Antimicrob Chemother. 67:2908-2911. (IF=4.480)
  • Mandalakas AM, Kirchner HL, Lombard C, Walzl G, Grewal HMS, Gie RP, Hesseling AC. (2012) Well-quantified tuberculosis exposure is a reliable surrogate measure of tuberculosis infection. Int J Tuberc Lung Dis. 16(8):1033–1039. (IF=2.426)
  • Mbugi EV, Katale BZ, Kendall S, Good L, Kibiki GS, Keyyu JD, Godfrey-Faussett P, van Helden PD, Matee MI. (2012) ‘Tuberculosis cross-species transmission in Tanzania: Towards a One-Health concept. Onderstepoort Journal of Veterinary Research. 79(2):1-6. (IF=0.890)
  • Mbugi EV, Kayunze KA, Katale BZ, Kendall S, Good L, Kibik GS, Keyyu JD, Godfrey-Fausett P, van Helden PD, Matee MI. (2012) ‘One Health’ infectious diseases surveillance in Tanzania: Are we all on board the same flight? Onderstepoort Journal of Veterinary Research. 79(2):1-7. (IF=0.890)
  • McEvoy CRE, Cloete R, Muller B, Schurch AC, van Helden PD, Gagneux S, Warren RM, Gey van Pittius NC. (2012) Comparative analysis of Mycobacterium tuberculosis PE and PPE genes reveals high sequence variation and apparent absence of selective constraints. Plos One 7(4): e30593. (IF=4.610)
  • Miller M, Joubert J, Mathebelu N, De Klerk- Lorist L, Lyashchenko KP, Bengis R, van Helden PD, Hofmeyer M. Olea–Popelka F, Greenwald R, Esfandiara J, Buss P. (2012) Detection of antibodies to tuberculosis antigens in free –ranging lions (Panthera Leo) infected with Mycobacterium bovis in Kruger National Park, South Africa. JZWM 43(2): 317-323. (IF=0.710)
  • Muller A, Möller M, Adams LA, Warren RM, Hoal EG, van Helden PD. (2012) Comparative analysis of putative TB-susceptibility gene, MC3R (Melanocortin-3-receptor), and pseudogene sequence in cattle (Bos taurus), African buffalo (Syncerus caffer), hyena (Crocuta crocuta), rhino (Ceratotherium simum) and other African bovids and ruminants. Cytogenet Gen Res. 136(2): 117-122. (IF=2.074)
  • Mukinda FK, Theron D, Van der Spuy GD, Jacobson KR, Roscher M, Streicher EM, Musekiwa A, Coetzee GJ, Victor TC, Marais BJ, Nachega JB, Warren RM, Schaaf HS. (2012) Rise in Rifampicin-monoresistant Tuberculosis in Western Cape, South Africa. Int J Tuberc Lung Dis 16(2): 196-202. (IF=2.426)
  • Parsons SDC, Menezes AM, Cooper D, Walzl G, Warren RM, van Helden PD. (2012) Development of a diagnostic gene expression assay for tuberculosis and its use under field conditions in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology. 148(3-4):337-342. (IF=2.221)
  • Parsons SDC, Warren RM, Ottenhoff THM, Gey van Pittius NC, Van Helden PD. (2012) Detection of Mycobacterium tuberculosis infection in dogs in a high-risk setting.  Res Vet Sci. 92(3): 414-419. (IF=1.544)
  • Riou C, Peixoto BP, Roberts L, Ronacher K, Walzl G, Manca C, Rustomjee R, Mthiyane T, Fallows D, Gray CM, Kaplan G. (2012) Effect of standard Tuberculosis treatment on Plasma Cytokine levels in patients with Active Pulmonary Tuberculosis. PLoS One. 7(5):e36886. (IF=4.610)
  • Robertson BD, Altmann D, Barry C, Bishai B, Cole S, Thomas Dick, Duncan K, Dye C, Ehrt S, Esmail H, Flynn J, Hafner R, Handley G, Hanekom W, van Helden PD, Kaplan G, Kaufmann SH, Kim P, Lienhardt C, Mizrahi V, Rubin E, Schnappinger D, Sherman D, Thole J, Vandal O, Walzl G, Warner D, Wilkinson R, Young D. (2012) Detection and treatment of subclinical tuberculosis. Tuberculosis (Edinb). 92 (6):447-452.(IF=3.149)
  • Seddon JA, Hesseling AC, Marais BJ, Jordaan AM, Victor TC, Schaaf HS. (2012) The evolving epidemic of drug-resistant tuberculosis among children in Cape Town, South Africa. Int J Tuberc Lung Dis. 16(7):928–933. (IF=2.426)
  • Seddon JA, Jordaan AM, Victor TC, Schaaf HS. (2012) Discordant Drug Susceptibility for Mycobacterium tuberculosis within Families. Pediatr Infect Dis J. 31(7): 783-785. (IF=3.271)
  • Seddon JA, Visser DH, Bartens M, Jordaan AM, Victor TC, van Furth AM, Schoeman JF, Schaaf HS. (2012) Impact of drug resistance on clinical outcome in children with tuberculous meningitis. Pedriatr Infect Dis J 31(7): 711-716. (IF=3.271)
  • Seddon JA, Warren RM, Enarson DA, Beyers N, Schaaf HS. (2012) Drug-resistant tuberculosis in children is caused by transmission and amplification of resistance within families. Emerg Infect Dis 18(8):1342-1345 (IF=6.996)
  • Sirgel FA, Tait M, Warren RM, Streicher EM, Böttger EC, van Helden PD, Gey Van Pittius NC, Coetzee G, Hoosain EY, Chabula-Nxiweni M, Hayes C, Victor TC, Trollip A. (2012) Mutations in the rrs A1401G Gene and Phenotypic Resistance to Amikacin and Capreomycin in Mycobacterium tuberculosis. Microb Drug Resist. 18(2): 193-197. (IF=1.847)
  • Sirgel FA, Warren RM, Streicher EM, Victor TC, van Helden PD, Böttger EC. (2012) gyrA mutations and phenotypic susceptibility levels to ofloxacin and moxifloxacin in clinical isolates of Mycobacterium tuberculosis. J Antimicrob Chemother. 67(5): 1088-1093. (IF=4.480)
  • Streicher EM, Bergval I, Dheda K, Bottger E, Gey van Pittius NC, Bosman M, Coetzee G, Anthony R, van Helden PD, Victor TC, Warren RM. (2012) Mycobacterium tuberculosis population structure determines the outcome of genetic based second-line drug testing. Antimicrob. Agents Chemother. 56(5): 2420-2427. (IF=4.680)
  • Streicher EM, Müller B, Chihota V, Mlambo C, Tait M, Pillay M, Trollip A, Hoek KGP, Sirgel FA, Gey van Pittius NC, Van Helden PD, Victor TC, Warren RM. (2012) Emergence and treatment of multidrug resistant (MDR) and extensively drug-resistant (XDR) tuberculosis in South Africa. Infection, Genetics and Evolution. 12(4):686–694. (IF=3.055)
  • Van Rie A, Mellet K, John M-A, Scott L, Page-Shipp L, Dansey H, Victor TC, Warren RM. (2012) False positive Rifampicin resistance on Xpert®MTB/RIF: case report and clinical implications. Int J Tuberc Lung Dis 16(2): 206-208. (IF=2.426)
  • Van Schalkwyk C, Cule M, Welte A, van Helden PD, van der Spuy G, Uys P. (2012) Towards eliminating bias in Cluster Analysis of TB Genotyped data. PloS One. 7(3):e34109 (IF=4.610)
  • Van Soelen N, Mandalakas AM, Kirchner HL, Walzl G, Grewal HM, Jacobsen M, Hesseling AC. (2012) Effect of Ascaris Lumbricoides specific IgE on tuberculin skin test responses in children in a high-burden setting: a cross-sectional community-based study. BMC Infect Dis. 12(1):211 (IF=3.236)
  • Visser ME, Stead MC, Walzl G, Warren RM, Schomaker M, Grewal HM, Swart EC, Maartens G. (2012) Baseline predictors of sputum culture conversion in pulmonary tuberculosis: importance of cavities, smoking, time to detection an w-beijing genotype. PloS One 7(1): e29588. (IF=4.610)
  • Weiner B, Gomez J, Victor TC, Warren RM, Sloutsky A, Plikatis BB, Posey JE, van Helden PD, Gey van Pittius NC, Koehrsen M, Sisk P, Stolte C, White J, Gagneux S, Birren B, Hung D, Murray M, Galagen J. (2012) Independent large scale duplications in multiple M. tuberculosis lineages overlapping the same genomic region. PloS One 7(2): e26038. (IF=4.610)
  • Zhu R, Kiser JJ, Seifart HI, Werely CJ, Mitchell CD, D’Argenio DZ, Fletcher CV. (2012) The Pharmacogenetics of NAT2 enzyme maturation in perinatally HIV exposed infants receiving isoniazid. J Clin Pharmacol. 52(4): 511-519. (IF=3.387)

Non Peer-Reviewed Articles (Total: 3)

  • Van Helden PD. (2012) Simplicity. EMBO reports 13:172. (IF=7.488)
  • Van Helden PD. (2012) The cost of research in developing countries. EMBO reports 13(5): 395. (IF=7.488)
  • Van Helden PD. (2012) Not too much and not too little. EMBO Reports. 13:942.

Published Abstracts (Total: 0)

Technical Reports (Total: 0)

Products / Artefacts / Patents (Total: 0)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 92)

Plenary/Keynote/ Distinguished Lectures

  • Mizrahi V. Development and Application of New Tools for TB Drug Discovery: A View from South Africa. Keynote lecture presented at the 4th Annual CEND Symposium: Fighting the Diseases of Poverty, Centre for Emerging and Neglected Diseases, UC Berkeley, 13 January 2012
  • Mizrahi V. New Tools for TB Drug Discovery. Plenary lecture delivered at the Symposium on Scientific Advances in TB Pathogenesis and Treatment, 10th Conference on Retroviruses and Opportunistic Infections (CROI 2012), Seattle, WA, 5-9 March 2012
  • Mizrahi V. Drug Resistance in TB: Scope of the problem and underlying mechanisms. Lecture presented at the pre-Symposium Workshop. Keystone Symposium on Drug Resistance and Persistence in Tuberculosis, Speke Resort, Kampala, Uganda, 13-18 May 2012
  • Mizrahi V. Mechanisms driving mutations to drug resistance. Plenary lecture presented at the Keystone Symposium on Drug Resistance and Persistence in Tuberculosis, Speke Resort, Kampala, Uganda, 13-18 May 2012
  • Mizrahi V. New Tools for TB Drug Discovery. Plenary lecture presented at the 3rd SA TB Conference 2012, Durban, 12-15 June 2012
  • Mizrahi V. Knowing the Enemy: Survival and Subversion Strategies of Mycobacterium tuberculosis. Inaugural Lecture, University of Cape Town, 22 August 2012.
  • Mizrahi V. New Tools for Tuberculosis Drug Discovery: A South African Perspective. Wolfson Colloquium, University of Cape Town, 4 September 2012.
  • Mizrahi V. Pathway-selective sensitization of Mycobacterium tuberculosis for use in target-based whole-cell screening. Invited lecture presented at the H3-D Symposium on New Paradigms in Drug Discovery: Challenges and Opportunities in Africa, 16 October 2012.
  • Kana B. Peptidoglycan remodelling during mycobacterial growth and persistence. Opening talk in the “Innovations from Africa Theme”. Kwazulu-Natal Research Institute for TB and HIV (K-RITH) Opening Scientific Symposium. Hilton Hotel, Durban, South Africa. 10th October 2012.

Invited Talks

  • Mizrahi V. New Tools for TB Drug Discovery. Invited lecture presented at the Department of Medicine Seminar Series, University of Cape Town, 15 March 2012
  • Evans J, Mizrahi V. Identifying vulnerable targets in the CoA biosynthetic pathway of M. tuberculosis. Lecture presented at the Kick-Off meeting, High-Quality Hits for Tuberculosis (HIT-TB) Consortium, Cambridge, UK, 27 March 2012
  • Mizrahi V. Mechanisms driving mutations to drug resistance. Lecture presented at the IIDMM/ICGEB Seminar Series, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, 9 May 2012
  • Singh V, Warner DF, Mizrahi V. Pyrimidine metabolism in M. tuberculosis – an update from UCT. Invited talk presented at the Fourth Consortium Meeting, More Medicines for Tuberculosis (MM4TB), Tällberg, Sweden, 5-6 July 2012
  • Gopinath K, McKinney JD, Mizrahi V, Warner DF. BacA is essential for vitamin B12 transport in M. tuberculosis. Invited talk presented at Tuberculosis 2012, Paris, 11-15 September, 2012
  • Gopinath K, McKinney JD, Mizrahi V, Warner DF. Identification of genes requires for transport and assimilation of vitamin B12 in M. tuberculosis. Invited talk presented at the Vitamin B12 Symposium, Nancy, France, 20-22 September 2012.
  • Mizrahi V. Pathway-selective sensitization of Mycobacterium tuberculosis for use in target-based whole-cell screening. Invited talk presented at the H3-D Symposium on New Paradigms in Drug Discovery, Cape Town, 16 October 2012.
  • Kana B. Remodelling the mycobacterial cell wall for new TB drugs. Invited talk at École polytechnique fédérale de Lausanne EPFL. Lausanne, Switzerland, 7th September 2012.
  • Kana B. Remodelling the mycobacterial cell wall for new TB drugs. Invited talk at the University of Leicester. Leicester, United Kingdom. 10th July 2012.
  • Kana B. Peptidoglycan remodelling during TB infection. Invited talk at the Kwazulu-Natal Research Institute for TB and HIV (K-RITH), Durban, South African, 31st May 2012.
  • Kana B. Career Guidance for young scientists and NRF ratings. Oral presentation at the Research Indaba. Midrand graduate institute. Johannesburg. 22nd October 2012.
  • Kana B. How to Build an Academic Career in Science. Oral presentation at the Research Capacity Building Session of the 3rd SA TB Conference. International Convention Centre, Durban, South Africa. 12-15 June 2012.
  • Kana B. Building an Academic Career in Science. Invited talk given at the Career Guidance for Young Scientists Workshop at theSASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Hassim F, Gordhan B. Construction and phenotypic characterization of M. smegmatis mutants deficient in the MutY DNA glycosylase. Oral presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Hassim F, Gordhan B. Construction and phenotypic characterization of M. smegmatis mutants deficient in the MutY DNA glycosylase. Oral presentation at the MRC Early Career Scientists Conference 2012, 24th – 25th October 2012.
  • Narrandes N, Mizrahi V, Kana B. Functional characterization of molybdopterin synthase encoding genes in mycobacteria. Oral presentation at the SASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Narrandes N, Mizrahi V, Kana B. Functional characterization of molybdopterin synthase encoding genes in mycobacteria. Oral presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Sensani S. My Journey at the CBTBR. Oral presentation at the DST/NRF Centre of Excellence Director’s Forum. Sanlam Centre, Pretoria, South Africa. 13th November 2012
  • Senzani S, Kana B. Analysis of peptidoglycan degrading amidases. Oral presentation at the MRC Early Career Scientists Conference 2012, 24th – 25th October 2012.
  • Moolla N, Gordhan B. Construction and Phenotypic Characterization of Mycobacterium smegmatis Mutants Deficient in the Nth DNA Glycosylase. Oral presentation at the MRC Early Career Scientists Conference 2012, 24th – 25th October 2012.
  • Ealand C, Kana B. The role of mycobacterial DD-carboxypeptidases in peptidoglycan remodeling and turnover. Oral presentation at the SASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Ealand C, Kana B. The role of mycobacterial DD-carboxypeptidases in peptidoglycan remodeling and turnover. Oral presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Ealand C, Kana B. The role of mycobacterial DD-carboxypeptidases in peptidoglycan remodeling and turnover. Oral and poster presentation at the MRC Early Career Scientists Conference 2012, 24th – 25th October 2012.
  • Hoal EG. Genomics of Infectious Diseases in Africa. Invited talk presented at the Golden Helix Symposia: Genomic Medicine: Translating Genes into Health. 18-21 April 2012. Turin, Italy.
  • Warren RM. Characterizing Drug-Resistant TB epidemics in South Africa. Invited talk presented at Stellenbosch University. 19th April 2012.   
  • Le Roux N. Host genetic susceptibility to M. bovis in buffalo. Talk presented at the Annual Bovine TB Workshop, Kruger National Park, May 2012.
  • Grobbelaar M. Adaptation of the Mycobacterium tuberculosis transcriptome in response to Rifampicin. Talk presented at the Keystone Symposia, Uganda during 13-18 May 2012.
  • Parsons SDC. Adaptation of the QuantiFERON TB-Gold assay for the diagnosis of TB in wildlife. Talk presented at the International Wildlife TB Conference, Skukuza, Kruger national Park, South Africa, May 2012.
  • Parsons SDC. Development of a Gene Expression Assay for the diagnosis of TB. Talk presented at a meeting of the BTB Study Group, Skukuza, KNP, September 2012.
  • Warren RM. Deciphering the Epidemiology of drug-resistant tuberculosis through comparative genomics. Invited speaker at the 6th Annual New England TB Symposium, Focus on Drug-resistance, Broad Institute, Cambridge, 28 June 2012.
  • Le Roux N. Novel SNP identification and association with bovine TB in African buffalo. Invited talk presented at the International Society of Animal Genetics (ISAG) 2012 conference, Cairns, Australia, 15-20 July 2012.
  • Klopper M. Molecular characterization of the drug-resistant tuberculosis epidemic in the Eastern Cape, South Africa. Talk presented at SACEMA Research day, Stellenbosch, April 2012.
  • Streicher EM. Mycobacterium tuberculosis population structure determines the outcome of genetic based second-line drug resistance testing. Talk presented at the Keystone Symposium, Drug Resistance and Persistence in Tuberculosis, Kampala Uganda, May 2012.
  • Fortuin S. The evolution of the Mycobacterium tuberculosis proteome. Invited speaker at the Welcome Trust All Directors Meeting, Accra, Ghana 2012.
  • Fortuin S. Identification of virulence proteins of Mycobacterium tuberculosis. Invited speaker at the Southern Africa Consortium for Research Excellence Annual Meeting, Gaborone, Botswana, September 2012.
  • Fortuin S. The phosphoproteomes of a hypo- and hyper-virulent clinical M. tuberculosis Beijing strains. Talk presented at the Stellenbosch University 56th Annual Academic Year day 2012, 15-16 August 2012.
  • Van Helden PD. Molecular Epidemiology of Mycobacterium TB. Invited talk presented at the SA Clinicians Society Conference 2012, Cape Town, South Africa, 25-28 November 2012.
  • Van Helden PD. Antibiotics and omics of TB. Invited talk presented at the Structural Biology in the Bio Economy Conference, Cape Town, South Africa, 2-4 December 2012.
  • Walzl G. TB biomarkers in peripheral blood. Invited talk presented at the 43rd Union World Conference on Lung Health, Kuala Lumpur Malaysia , 13-17 November 2012.
  • Walzl G. Clinical impact of recent advances for the prevention, diagnosis and treatment of tuberculosis. Invited talk presented at the European Respiratory Society Annual Congress, 1-5 September 2012, Vienna, Austria.
  • Walzl G. Lessons from the search for biomarkers for active TB and TB treatment response.  Invited talk presented at the Joint EVI/TBVI Symposium. Les Diablerets, Switzerland, 31 January 2012.
  • Walzl G. Utility of multiplex cytokine arrays in TB biomarker discovery. Invited talk presented at the Tuberculosis Research Unit Annual meeting, Cleveland, USA, 26-27 November 2012.
  • Le Roux N. Novel SNP identification and association with bovine TB in African buffalo. Talk presented at the International Wildlife Tuberculosis Conference, Kruger National Park, South Africa, 9-12 September 2012.
  • Werely CJW. TB treatment considerations for neonates and infants. Talk presented at the 43rd Union World Conference on Lung Health, Kuala Lumpur, Malaysia, 13-17 November 2012.

Posters

  • Koch, A., Mizrahi, V., Warner, D.F. Physiology of drug-resistant mycobacteria: implications for pathogenesis. Poster presented at the Keystone Symposium on Drug Resistance and Persistence in Tuberculosis, Speke Resort, Kampala, Uganda, 13-18 May 2012.
  • Mapela L, Beukes G, Senzani S, Kana B. Peptidoglycan remodelling in mycobacterial growth and pathogenesis. Poster presentation at the Tuberculosis 2012: Biology, pathogenesis, intervention strategies. Institut Pasteur, Paris, France, 11th-15th September 2012.
  • Kana B. Resuscitation promoting factors in bacterial growth and cell wall remodelling - Extreme Makeover for the Cell wall.  Poster presentation at the Annual HHMI Scholars Meeting. HHMI Headquarters, Chevy Chase, Maryland, USA. 14-17 February 2012.
  • Asmal R, Kana B. Identification and cellular localization of DD-Carboxypeptidase-interacting proteins in Mycobacterium smegmatis. Poster presentation at the 4th Cross Faculty Graduate Symposium: Showcasing Postgraduate Research at Wits 2012. Wits University – Professional Development Hub, Johannesburg, South Africa. 19th and 22nd October.
  • Asmal R, Kana B. Identification and cellular localization of DD-Carboxypeptidase-interacting proteins in Mycobacterium smegmatis. Poster presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Hassim F, Gordhan B. Construction and phenotypic characterization of M. smegmatis mutants deficient in the MutY DNA glycosylase. Poster presentation at the SASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Narrandes N, Mizrahi V, Kana B. Functional characterization of molybdopterin synthase encoding genes in mycobacteria. Poster presentation at the Tuberculosis 2012: Biology, pathogenesis, intervention strategies. Institut Pasteur, Paris, France, 11th-15th September 2012.
  • Narrandes N, Mizrahi V, Kana B. Functional characterization of molybdopterin synthase encoding genes in mycobacteria. Poster presentation at the 4th Cross Faculty Graduate Symposium: Showcasing Postgraduate Research at Wits 2012. Wits University – Professional Development Hub, Johannesburg, South Africa. 19th and 22nd October.
  • Senzani S, Kana B. Analysis of peptidoglycan degrading amidases. Poster presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Senzani S, Kana B. The role of resuscitation promoting factors in peptidoglycan hydrolysis and reactivation from dormancy in Mycobacterium smegmatis. Poster presentation at the SASBMB/FASBMB Congress 2012. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Beukes G, Mapela L, Kana B. The role of resuscitation promoting factors in peptidoglycan hydrolysis and reactivation from dormancy in Mycobacterium smegmatis. Poster presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Moolla N, Gordhan B. Construction and Phenotypic Characterization of Mycobacterium smegmatis Mutants Deficient in the Nth DNA Glycosylase. Poster presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Moolla N, Gordhan B. Construction and Phenotypic Characterization of Mycobacterium smegmatis Mutants Deficient in the Nth DNA Glycosylase. Poster presentation at the SASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Goosens V, Mizrahi V, Gordhan B. The role of DNA glycosylases in mutagenesis and adaptation to stress in mycobacteria. Poster presentation at the Keystone Symposia |Drug resistance and persistence in tuberculosis. Kampala, Uganda. 13th – 18th May 2012.
  • Chengalroyen M, Kana B. The role of resuscitation promoting factors in the reactivation of Mycobacterium tuberculosis in sputum. Poster presentation at the Wits Health Sciences Research Day & Postgraduate Expo 2012. Wits Medical School, Johannesburg, South Africa, 19th September 2012.
  • Chengalroyen M, Kana B. A tool to improve the diagnosis of Mycobacterium tuberculosis. Poster presentation at the Spring Science Showcase. Delta Environmental Centre, Johannesburg, South Africa, 9th September 2012.
  • Chengalroyen M, Kana B. The role of resuscitation promoting factors in the reactivation of Mycobacterium tuberculosis in sputum. Poster presentation at the SASBMB/FASBMB Congress 2012 - The South African Society of Biochemistry and Molecular Biology. Champagne Sports Resort, Drakensberg, KwaZulu-Natal, 29 January – 1 February 2012.
  • Moller M. A CD14 promoter polymorphism is implicated in tuberculosis susceptibility in a South African population. Poster presented at the 62nd Annual Meeting of the American Society of Human Genetics, San Francisco, USA, 6 – 10 November 2012.
  • Daya M. A panel of ancestry informative markers for the South African Coloured population. Poster presented at the 62nd Annual Meeting of the American Society of Human Genetics, San Francisco, USA, 6-10 November 2012.
  • Salie M. HLA class-I supertypes are associated with specific M. tuberculosis strain infections. Poster presented at the 62nd Annual Meeting of the American Society of Human Genetics, San Francisco, USA, 6-10 November 2012.
  • De Vos M. Increasing levels of rifampicin resistance in Mycobacterium tuberculosis results in minor changes in the genomes of in vitro rifampicin resistant mutants. Poster presented at Keystone Symposium: E1, Drug Resistance and Persistence in Tuberculosis, Kampala, Uganda, May 2012.
  • Black PA. Regulatory response to Rifampicin in multi-drug resistant Mycobacterium tuberculosis strains. Poster presented at the Keystone Symposia on Drug Resistance and Persistence in Tuberculosis, May 2012.
  • Salie M. Poster presented at the EMBO|EMBL Symposium: New Perspectives on Immunity to Infection, Heidelberg, Germany, 19-22 May 2012.
  • Louw GE. Mycobacterium tuberculosis sputum cultures show polyphenotypic rifampicin resistance. Poster presented at Keystone Symposium, Drug Resistance and Persistence in Tuberculosis, Kampala Uganda, May 2012.
  • Le Roux. Novel SNP identification and association with bovine TB in African buffalo. Poster presented at the International Society of Animal Genetics (ISAG) 2012 conference, Cairns, Australia, 15-20 July 2012.
  • Willemse D. Regulation of efflux in rifampicin resistant mutants of Mycobacterium tuberculosis. Poster presented at the EMBO Tuberculosis 2012: Biology, pathogenesis, intervention strategies Conference, Institut Pasteur, Paris, France, 11-15 September 2012.
  • McGrath M. Nucleoside analogs and their possible role in the emergence of TB drug resistance.  Poster presented at the EMBO Tuberculosis: Biology, Pathogenesis, Intervention strategies Conference, Paris, France, 11-15 September 2012.
  • Viljoen A. Genetic study of the nitrogen assimilatory pathways of Mycobacterium bovis BCG. Poster presented at the EMBO Tuberculosis: Biology, Pathogenesis, Intervention strategies Conference, Paris, France, 11-15 September 2012.
  • Sao Emani C. The role of ergothioneine in the physiology of Mycobacterium smegmatis. Poster presented at the EMBO Tuberculosis: Biology, Pathogenesis, Intervention strategies Conference, Paris, France, 11-15 September 2012.
  • Fortuin S. A phosphoproteomic approach to characterise mechanisms of virulence in clinical M. tuberculosis Beijing strains. Poster presented at the Keystone Symposia (Proteomics, Interactomes), Stockholm, Sweden 2012. 
  • Fortuin S. The phosphoproteomes of a hypo- and hyper-virulent clinical M. tuberculosis Beijing strains. Poster presented at the MRC Early Career Scientist Conference, 25 October 2012.
  • Fang Z. Mycosin-3 of Mycobacterium tuberculosis, functional and structural studies.  Poster presented at the Structural Biology in the Bio Economy Conference, Cape Town, South Africa, 2-4 December 2012.
  • Parsons SDC. Development of a diagnostic gene expression assay for tuberculosis in African buffaloes (Syncerus caffer). Poster presented at the 8th Conference of the Federation of African Immunological Societies, Durban, December 2012.
  • Parsons SDC. A novel “dassie bacillus” variant isolated from meerkats. Poster presented at the International Wildlife TB Conference, Skukuza, KNP, September 2012.
  • Dippenaar A. Evolution of the members of the group 2 Latin American-Mediterranean (LAM) genotype of M. Tuberculosis. Poster presented at EMBO course:  Computational Biology: from genomes to cells and systems, 14-20 October 2012.
  • Ngwane A. Identification of a novel anti- M. tuberculosis compound (F1082) for TB drug development. Poster presented at Keystone Symposium, Drug Resistance and Persistence in Tuberculosis, Kampala Uganda, May 2012.
  • Ngwane A. Evaluation of Vav1Contribution in B cell Activation and Immunosuppression.  Poster presented at Novartis Next Generation of Scientist Meeting, 23 August 2012.
  • Loxton A. The frequency of CD103 as a marker of regulatory CD4+ T-cells and suppressor CD8+ T-cells is not affected by ARVs, but has a negative correlation with absolute CD4 cell numbers. Poster presented at the XIX International AIDS Conference, Washington DC, USA, 22-27 July 2012.
  • Loxton A. Regulatory T-cells and high levels of FOXP3 mRNA lead to decreased immune responses during HIV-TB co-infection .Poster presented at European Respiratory Society (ERS) 2012 Conference, Vienna, Austria, 1-5 September 2012.

Other Relevant Outputs (including honours and awards to staff)

  • SARCHi Research Chairs: The NRF has provisionally allocated 3 new SARCHi’s to the CBTBR in 2012, evidence of the strength of the CBTBR and the NRF’s confidence in the entity
  • Dr Bavesh Kana was selected as an Early Career Scientist of the Howard Hughes Medical Institute after a fiercely competitive process which involved almost 800 applicants worldwide. Dr. Kana is one of only 26 scientists from around the world to receive this prestigious award and represents one of only two awards made in Africa. The award comes with a $ 715 000 research grant, over the period of five years, Further details for this award can be found at http://www.hhmi.org/research/iecs/
  • Dr. Bavesh Kana was appointed as one of the top 200 Young South Africans by the Mail and Guardian newspaper. The Mail and Gaurdian 200 Young South African list comprises those individuals who are expected to make a significant impact in South Africa and play a key leadership role in the country. More details on this can be found at http://ysa2012.mg.co.za/
  • Dr Bhavna Gordhan was honored for her research achievements and leveraging significant research funding at the 2012 Annual Faculty of Health Sciences Research Awards.
  • Dr Bhavna Gordhan was awarded the Keystone Symposia Global Health Travel Award to attend the Keystone Symposium on Drug resistance and persistence in tuberculosis. Kampala, Uganda. 13th – 18th May 2012.
  • Dr Bavesh Kana was selected as an Early Career Scientist of the Howard Hughes Medical Institute after a fiercely competitive process which involved almost 800 applicants worldwide. Dr. Kana is one of only 26 scientists from around the world to receive this prestigious award and represents one of only two awards made in Africa. The award comes with a $ 715 000 research grant, over the period of five years, Further details for this award can be found at http://www.hhmi.org/research/iecs/
  • Prof. Mizrahi was selected as a Senior International Research Scholar of the HHMI and awarded five-year a $500,000 research grant. This highly prestigious SIRS award was given to 13 basic science researchers from countries outside the USA. This is Prof. Mizrahi’s third award from the HHMI. Details of this award can be found at http://www.hhmi.org/news/SIRS20120926.html. Dr. Williams was awarded a Columbia University-Southern African Fogarty AITRP Traineeship and a PHRI-AURUM-Global Infectious Diseases Research Fogarty Traineeship, July-Dec 2012
2011

* The Names in bold are CBTBR staff

Books / Chapters in Books (Total: 4)

  • *Muller B, Warren, RM, Williams M, Bottger EC, Gey van Pittius NC, Victor TC. 2011. Acquisition, transmission and amplification of drug-resistant tuberculosis. In: Antituberculosis chemotherapy. Progress in Respiratory Research, volume 40, Karger, pp. 96–104.
  • Donald PR, van Helden PD. Progress in Respiratory Research – Vol 40, Antituberculosis Chemotherapy. Edited by Peter R Donald, and Paul D van Helden. Published by S Karger AG, Basel, Switzerland, 2011. ISBN 978-3805-596275.
  • Williams M, Müller B, Uys P, Victor TC, Warren RM, Gey van Pittius NC. (2011) Tuberculosis Recurrence: Exogenous or Endogenous? In: Antituberculosis chemotherapy. Progress in Respiratory Research, volume 40, Karger, pp 73-80.
  • Diacon AH, Maritz JS, Donald PR. (2011) Early bactericidal activity of antituberculosis agents. In: Antituberculosis chemotherapy. Progress in Respiratory Research, volume 40, Karger, pp 213-219.

Articles in Peer-Reviewed Journals (Total: 47)

  • Scott LE, Gous N, Cunningham BE, Kana BD, Perovic O, Erasmus L, Coetzee G, Koornhof H, Stevens W. 2011. Dried Culture Spots for Xpert MTB/RIF External Quality Assessment: Results of phase 1 pilot study from South Africa. J Clin Microbiol. 49:4356-4360. [IF=4.220]
  • Kondratieva T, Rubakova E, Kana BD, Biketov S, Potapov V, Kaprelyants A, Apt A. 2011. Mycobacterium tuberculosis attenuated by multiple deletions of rpf genes effectively protects mice against TB infection. Tuberculosis (Edinb) 91:219-223. [IF=2.650]
  • Ahmadou Ahidjo B, Kuhnert D, McKenzie J, Machowski EE, Gordhan BG, Arcus VL, Abrahams GL, Mizrahi V. 2011. VapC toxins from Mycobacterium tuberculosis are ribonucleases that inhibit mycobacterial growth and are neutralized by cognate VapB antitoxins. PLoS ONE6:e21738. [IF=4.411]
  • Warner DF, Mizrahi V. 2011. Making Ends Meet in Mycobacteria. Mol. Microbiol. 79:283-287. [MicroCommentary] [IF=4. 819]
  • Williams MJ, Kana BD, Mizrahi V. 2011. Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a novel fused molybdopterin synthase in Mycobacterium tuberculosis. J. Bacteriol. 193:98-106. [IF=3.726]
  • Adams LA, Möller M, Nebel A, Schreiber S, Van der Merwe L, Van Helden PD, Hoal EG. (2011) Polymorphisms in MC3R promoter and CTSZ 3'UTR are associated with tuberculosis susceptibility. Eur J Hum Genet. 19: 676-681. [IF=4.380]
  • Berg S, Garcia-Pelayo MC, Müller B, Hailu E, Asiimwe B, Kremer K, Dale J, Boniotti MB, Rodriguez S, Hilty M, Rigouts L, Firdessa R, Machado A, Mucavele C, Ngandolo BNR, Bruchfeld J, Boschiroli L, Muller A, Sahraoui N, Pacciarini M, Cadmus S, Joloba M, Van Soolingen D, Michel AL, Djonne B, Aranaz A, Zingsstag J, Van Helden P, Prtaels F, Kazwala R, Kallenius G, Hewinson RG, Aseffa A, Gordon SV, Smith NH. (2011) African 2, a clonal complex in Mycobacteruim bovis epidemiologically important in East Africa. Journal of Bacteriology. 193(3): 670-678. [IF=3.726]
  • Carugati M, Zanini F, Schiroli C, Gori A, Franzetti F, Hanekom M, Van der Spuy GD, Gey van Pittius NC, McEvoy CR, Ndabambi SL, Victor TC, Hoal EG, van Helden PD, Warren RM. (2011) Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeat Analysis and Beijing/W Family of Mycobacterium tuberculosis. J Clin Microbiol. 49(7): 2780-2781. . [IF=4.220]
  • Chegou NN, Hoek KG, Kriel M, Warren RM, Victor TC, Walzl G.(2011) Tuberculosis assays: past, present and future. Expert Rev Anti Infect Ther. 4: 457-469. [IF=3.090]
  • De Souza GA, Arntzen MO, Fortuin S, Schürch AC, Malen H, McEvoy CR,  Van Soolingen D, Thiede B, Warren RM, Wiker HG. (2011) Proteogenomic analysis of polymorphisms and gene annotation divergences in prokaryotes using a clustered mass spectrometry-friendly database. Mol & Cell Proteomics. 10: 10.1074/mcp.M110.002527 1-10. [IF=8.354]
  • De Wit E, Van der Merwe L, Van Helden PD, Hoal EG. (2011) Gene-gene interaction between tuberculosis candidate genes in a South African population. Mammalian Genome. 22: 100-110. [IF=2.771]
  • Diacon AH, Dawson R, Hanekom M, Narunsky K, Venter A, Hittel N, Geiter LJ, Wells CD, Paccaly AJ, Donald PR. (2011) Early bactericidal activity of delamanid (OPC-67683) in smear-positive pulmonary tuberculosis patients. Int J Tuberc Lung Dis. 15(7): 949-54. [IF=2.557]
  • Donald PR, Maritz JS, Diacon AH. (2011) The pharmacokinetics and pharmacodynamics of rifampicin in adults and children in relation to the dosage recommended for children. Tuberculosis. 91: 196-207. [IF=2.650]
  • Friedrich SO, Venter A, Kayigire XA, Dawson R, Donald PR, Diacon AH. (2011) Suitability of Xpert MTB/RIF and Genotype MTBDRplus for patient selection for a tuberculosis clinical trial. J Clin Micro. 49(8): 2827-2831. [IF=4.220]
  • Govender L, Abel B, Hughes EJ, Scriba TJ, Kagina BM, De Kock M, Walzl G, Black G, Rosenkrands I, Hussey GD, Mahomed H, Andersen P, Hanekom WA. (2011) Higher human CD4 T cell response to novel Mycobacterium tuberculosis latency associated antigens Rv2660 and Rv2659 in latent infection compared with tuberculosis disease. Vaccine. 29(1): 51-57. [IF=3.572]
  • Hanekom M, Gey van Pittius NC, McEvoy C, Victor TC, Van Helden PD, Warren RM. (2011) Mycobacterium tuberculosis Beijing genotype: A template for success. Tuberculosis. 91: 510-523. [IF=2.650]
    Hans RH, Wiid IJF, Van Helden PD, Wan B, Franzblau SG, Gut J, Rosenthal PJ, Chibale K. (2011) Novel thiolactone-isatin hybrids as potential antimalarial and antitubercular agents. Bioorganic & Medicinal Chemistrly Letters. 21: 2055-2058. [IF=2.661]
  • Hoek KGP, Van Rie A, Van Helden PD, Warren RM, Victor TC. (2011) Detecting Drug Resistant Tuberculosis: The Importance of Rapid Testing. Molecular Diagnosis & Therapy. 15(4): 189-194. [IF=2.561]
  • Honeyborne I, McHugh TD, Phillips PPJ, Bannoo S, Bateson A, Carroll N, Perrin FM, Ronacher K, Wright L, van Helden PD, Walzl G, Gillespie SH. (2011) Molecular Bacterial Load Assay, a culture-free biomarker for rapid and accurate quantification of sputum Mycobacterium tuberculosis bacillary load during treatment. J Clin Micro. 49(11): 3905-3911. [IF=4.220]
  • Jacobson KR, Theron D, Victor TC, Streicher EM, Warren RM, Murray MB. (2011) Treatment Outcomes of Isoniazid-Resistant Tuberculosis Patients, Western Cape Province, South Africa. Clinical Infectious Diseases. 53(4): 369-372. [IF=8.186]
  • Jacobsen M, Repsilber D, Kleinsteuber K, Gutschmidt A, Schommer-Leitner S, Black G, Walzl G, Kaufmann SHE. (2011) Suppressor of cytokine signaling-3 is affected in T cells from TuberculosisTB patients. Clin Microbiol Infect. 17(9): 1323-1331. [IF=4.784]
  • Jeon CY, Calver AD, Victor TC, Warren RM, Shin SS, Murray MB. (2011) Use of fluoroquinolone antibiotics leads to tuberculosis treatment delay in a South African gold mining community. Int J Tuberc Lung Dis. 15(1): 77-83. [IF=2.557]
  • Kleynhans L, Du Plessis N, Black GF, Loxton AG, Kidd M, Van Helden PD, Walzl G, Ronacher K. (2011) Medroxyprogesterone acetate alters Mycobacterium bovis BCG-induced cytokine production in peripheral blood mononuclear cells of contraceptive users. Plos ONE. 6(9): e24639. [IF=4.411]
  • Laisse CJM, Gavier-Widén D, Ramis G, Bila CG, Machado A, Quereda JJ, Ågren EO, Van Helden PD. (2011) Characterization of tuberculous lesions in naturally infected African buffalo (Syncerus caffer). J Vet Diagn Invest. 23(5): 1022-1027. [IF=1.381]
  • Ligthelm LJ, Nicol MP, Hoek KGP, Jacobson R, Van Helden PD , Marais BJ, Warren RM,  Wright CA. (2011) Xpert® MTB/RIF for the rapid diagnosis of tuberculous lymphadenitis from Fine Needle Aspiration biopsy specimens. J Clin Micro. 49(11): 3967-3970. [IF=4.220]
  • Louw GE, Warren RM, Gey van Pittius NC, Leon R, Jimenez A, Hernandez-Pando R, McEvoy CRE, Grobbelaar M, Murray M, Van Helden PD, Victor TC. (2011) Rifampicin reduces susceptibility to Ofloxacin in Rifampicin-resistant Mycobacterium tuberculosis through efflux. Am J Respir Crit Care Med. 184: 269-276. [IF=10.191]
  • Maertzdorf J, Repsilber D, Parida SK, Stanley K, Roberts T, Black G, Walzl G, Kaufmann SH. (2011) Human gene expression profiles of susceptibility and resistance in tuberculosis. Genes Immun. 12:15-22. [IF=4.637]
  • Matthews K, Wilkinson KA, Kalsdorf B, Roberts T, Diacon A, Walzl G, Wolske J, Ntsekhe M, Syed F, Russel J, Mayosi BM, Dawson R, Dheda K, Wilkinson RJ, Hanekom WA, Scriba TJ. (2011). Predominance of interleukin-22 over interleukin-17 at the site of disease in human tuberculosis. Tuberculosis. 91(6): 587-593. [IF=2.650]
  • Mestre O, Luo T, Dos Vultos T, Kremer K, Murray A, Namouchi A, Jackson C, Rauzier J, Bifani P, Warren RM, Rasolofo V, Mei J, Gao Q, Gicquel B. (2011) Phylogeny of Mycobacterium tuberculosis Beijing strains constructed from polymorphisms in genes involved in DNA replication, recombination and repair. Plos ONE. 6(1): e16020. [IF=4.411]
  • Mkhize-Kwitshana ZL, Taylor M, Jooste P, Mabaso ML, Walzl G. (2011) The influence of different helminth infection phenotypes on immune responses against HIV in co-infected adults in South Africa. BMC Infect Dis. 11(1): 273. [IF=2.825]
  • Müller B, De Klerk-Lorist L-M, Henton MM, Lane E, Parsons SDC, Gey van Pittius NC, Kotze A, Van Helden PD, Tanner M. (2011) Mixed infections of Corynebacterium pseudotuberculosis and non-tuberculous mycobacteria in South African antelopes presenting with tuberculosis-like lesions. Vet Micro. 147: 340-345. [IF=3.256]
  • Müller B, Streicher EM, Hoek KGP, Tait M, Trollip A, Bosman ME,  Coetzee GJ, Chabula-Nxiweni EM, Hoosain E, Gey van Pittius NC, Victor TC, Van Helden PD, Warren RM. (2011) inhA promoter mutations: a gateway to extensively drug-resistant tuberculosis in South Africa? Int J Tuberc Lung Dis. 15(3): 344-351. [IF=2.557]
  • Nachega JB, Rosenkranz B, Simon G, Chaisson RE, Diacon AH, Taljaard JJ. (2011) Management of adult active tuberculosis disease in era of HIV pandemic, current practices and future perspectives. Infect Disord Drug Targets. 11(2): 134-143. [IF=3.061]
  • Onajole OK, Sosibo S, Govender P, Govender T, Van Helden PD, Maguire GEM, Mlinarić-Majerski K, Wiid IJF, Kruger HG. (2011) Novel linear diamine disubstituted polycyclic "cage" derivatives as potential anti-mycobacterial candidates. Chem Biol Drug Des. 78: 1022-1030. [IF=2.527]
  • Parsons SDC, Cooper D, McCall AJ, McCall WA, Streicher EM, Le Maitre NC, Müller A, Gey van Pittius NC, Warren RM, Van Helden PD. (2011) Modification of the QuantiFERON-TB Gold (In-Tube) assay for the diagnosis of Mycobacterium bovis infection in African buffaloes (Syncerus caffer). Veterinary Immunology and Immunopathology. 142: 113-118. [IF=2.176]
  • Schürch AC, Kremer K, Warren RM, Hung NV, Zhao Y, Wan K, Boeree MJ, Siezen RJ, Smith NH, Van Soolingen D. (2011) Mutations in the regulatory network that underlie the recent clonal expansion of a dominant subclone of the Mycobcterium tuberculosis Beijing genotype. Infection, Genetics and Evolution. 11: 587-597. [IF=3.086]
  • Schürch AC, Kremer K, Hendriks ACA, Freyee B, McEvoy CRE, Van Crevel R, Boeree MJ, Van Helden PD, Warren RM, Siezen RJ, Van Soolingen D. (2011) SNP/RD typing of Mycobacterium tuberculosis Beijing strains reveals local and worldwide disseminated clonal complexes. PLoS ONE. 6(12): e28365. [IF=4.411]
  • Shanaube K, Hargreaves J, Fielding K, Schaap A, Lawrence K-A, Hensen B, Sismanidis C, Menezes A, Beyers N, Ayles H, Godfrey-Faussett P. (2011) Risk Factors Associated with Positive QuantiFERON-TB Gold In-Tube and Tuberculin Skin Tests Results in Zambia and South Africa. PLoS ONE 6(4): e18206. [IF=4.411]
  • Smith NH, Berg S, Dale J, Allen A, Rodriguez S, Romero B, Matos F, Ghebremichael S, Karoui C, Donati C, Machado AC, Mucavele C, Kazwala RR, Hilty M, Cadmus S, Ngandolo BNR, Habtamu M, Oloya J, Muller A, Milian-Suazo F, Andrievskaia O, Projahn M, Barandiarán S, Macías A, Müller B, Zanini MS, Ikuta CY, Rodriguez CAR, Pinheiro SR, Figueroa A, Cho S-N, Mosavari N, Chuang P-C, Jou R, Zinsstag J, Van Soolingen D, Costello E, Aseffa A, Proaño-Perez F, Portaels F, Rigouts L, Cataldi AA, Collins DM, Boschiroli ML, Hewinson RG, Ferreira Neto JS, Surujballi O, Tadyon K, Botelho A, Zárraga AM, Buller N, Skuce R, Michel A, Aranaz A, Gordon SV, Jeon B-Y, Källenius G, Niemann S, Boniotti MB, Van Helden PD, Harris B, Zumárraga MJ, Kremer K. (2011) European 1: A globally important clonal complex of Mycobacterium bovis. Infection, Genetics and Evolution. 11: 1340-1351. [IF=3.086]
  • Tadokera R, Meintjies G, Skolimowska KH, Wilkinson KA, Matthews K, Seldon R, Chegou NN, Maartens G, Rangaka MX, Rebe K, Walzl G, Wilkinson RJ. (2010) Hypercytokinaemia accompanies HIV-tuberculosis immune reconstitution inflammatory syndrome. Eur Resp J. 37 (5): 1248-1259. [IF=5.922]
  • Thee S, Seddon JA, Donald PR, Seifart HI, Werely CJ, Hesseling AC, Rosenkrantz B, Roll S, Magdorf K, Schaaf HS. (2011) Pharmacokinetics of isoniazid, rifampicin and pyrazinamide in children younger than two years of age with tuberculosis: evidence for implementation of revised world health organization recommendations. Antimicrobial Agents and Chemotherapy. 55(12): 5560-5567. [IF=4.672]
  • Theron G, Peter J, Van Zyl-Smit R, Mishra H, Streicher E, Murray S, Dawson R, Whitelaw A, Hoelscher M, Sharma S, Pai M, Warren R, Dheda K. (2011) Evaluation of the Xpert MTB/Rif Assay for the diagnosis of pulmonary tuberculosis in a high HIV prevalence setting. Am J Respir Crit Care Med. 184: 132-140. [IF=10.191]
  • Van der Spuy DA, Cader S, Van der Spuy GD, Westwood AT. (2011) Improving nutritional status of children with cystic fibrosis at Red Cross War Memorial Children's Hospital. J Paediatr Child Health. 47: 282-286. [IF=1.122]
  • Van Wyk AC, Marais BJ, Warren RM, Van Wyk SS, Wright CA. The use of light-emitting diode fluorescence to diagnose mycobacterial lymphadenitis in fine-needle aspirates from children. Int J Tuberc Lung Dis. 15(1): 56-60. [IF=2.557]
  • Visser ME, Grewal HM, Swart EC, Dhansay MA, Walzl G, Swanevelder S, Lombard C, Maartens G. (2011) The effect of vitamin A and zinc supplementation on treatment outcomes in pulmonary tuberculosis: a randomized controlled trial. Am J Clin Nutr. 93(1): 93-100. [IF=6.606]
  • Walzl G, Ronacher K, Hanekom W, Scriba TJ, Zumla A. (2011) Immunological biomarkers of Tuberculosis. Nature Rev Immunol. 11(5): 343-354. [IF=35.196]
  • Warren RM, Hoek K, Sirgel F, Tait M, Gey van Pittius NC, Müller B, Streicher EM, Victor TC, Van Helden PD. (2011) Emergence and treatment of drug resistant tuberculosis: A comedy of errors. South African Respiratory J. 16(4): 112-116. [IF=Not Available]

Non Peer-Reviewed Articles (Total: 4)

  • Van Helden PD. (2011) Adaptation, redundancy or resilience. EMBO Reports. 12(9): 872.
  • Van Helden PD. (2011) Algorithms and surrogate markers in translational research. EMBO Reports. 12(12): 1206.
  • Van Helden PD. (2011) Treasure our rarities. EMBO Reports. 12(7): 611.
  • Van Helden PD. (2011) Water. EMBO Reports. 12(1): 2.

Published Abstracts (Total: 0)

Technical Reports (Total: 0)

Products / Artefacts / Patents (Total: 0)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 69)

  • Kana B. Mycobacterial heterogeneity in TB Infection - Extreme makeover for the cell wall. Howard Hughes Medical Institute Early Career Scientist Competition. Janelia Farm Research Campus, Ashburn, Virginia, USA, 7 November.
  • Kana B. Mycobacterial heterogeneity in TB Infection - Extreme makeover for the cell wall. Invited Plenary Lecture. MRC Research Day, MRC Conference Centre, Parow, Cape Town, 19-20 October.
  • Kana B. Remodelling the mycobacterial cell wall for better TB drugs. Invited oral presention. 4th Federation of Infectious Diseases Societies of Southern Africa, Durban, 8-11 September.
  • Beukes G, Mapela L, Kana B. The role of resuscitation promoting factors in peptidoglycan hydrolysis and reactivation from dormancy in Mycobacterium smegmatis. Oral Presentation. MRC Research Day, MRC Conference Centre, Parrow, Cape Town, 19-20 October.
  • Narrandes N, Mizrahi V., Kana B. Functional characterization of molybdopterin synthase encoding genes in mycobacteria. Oral Presentation. MRC Research Day, MRC Conference Centre, Parow, Cape Town, 19-20 October.
  • Gordhan B. The role of DNA glycosylases in mutagenesis and adaptation to stress in mycobacteria. Oral Presentation. MRC Research Day, MRC Conference Centre, Parrow, Cape Town, 19-20 October.
  • Williams M, Kana B, Mizrahi V. Phenotypic characterization of ∆mobA mutants of Mycobacterium tuberculosis and Mycobacterium smegmatis. Talk presented at the South African Society of Microbiology (SASM) Conference, Cape Town, 6-9 November 2011.
  • Mizrahi V. New tools for tuberculosis drug discovery: the development of novel screening strains of M. tuberculosis and application in target-based approaches. Invited lecture presented at the CIDRI and Imperial College Wellcome Centre for Clinical Tropical Medicine Joint Annual Meeting, University of Cape Town, 12-14 March 2011.
  • Abrahams GL, Savvi S, Kumar A, Hung A, Ciulli A, Sherman DR, Barry CE III, Boshoff HIM, Mizrahi V. The development of novel screening Strains of M. tuberculosis for use in target-led approaches to tuberculosis drug discovery. Plenary lecture delivered at the Eighth International Conference on the Pathogenesis of Mycobacterial Infections, Saltsjöbaden, Sweden, 29 June – 3 July, 2011.
  • Mizrahi V. The development of novel screening strains of M. tuberculosis for use in target-led approaches to tuberculosis drug discovery. Plenary lecture delivered at the K-RITH Groundbreaking Symposium, Durban, 13-14 July 2011.
  • Gopinath K, Mizrahi V, Warner DF. Identification of genes required for vitamin B12 transport in M. tuberculosis.  Oral presentation delivered at the Fourth Postgraduate Research Day, Faculty of Health Sciences, University of Cape Town.  7 September 2011.
  • Warner DF. Mycobacterial metabolism and human disease. Plenary lecture presented at the FIDSSA Congress, Durban, South Africa, 8-11 September 2011.
  • Gopinath K, Mizrahi, V., and Warner, D.F. Identification of genes required for vitamin B12 transport in M. tuberculosis. UCT Postdoctoral Retreat, Cape Town, 12 -13 September 2011.
  • Walzl G. Biomarkers for different forms of tuberculosis. Keystone Symposia. Vancouver, Canada. 16-20 January 2011 (Co-organiser and speaker at conference)
  • Hoal EG. Association of toll-like receptor 1, 2, 4, 8 and 9 genes with susceptibility to pulmonary tuberculosis. Invited talk presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Warren RM. Stellenbosch University, South Africa: New molecular epidemiology insights of tuberculosis in Southern Africa. Invited talk presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Le Roex N. Host genetics and disease susceptibility. Invited talk presented at WOTRO Midterm Symposium. Utrecht, Netherlands, 27-29 June 2011.
  • Victor TC. High infection pressure: implications on the TB epidemic in South Africa. Invited talk presented at the University of Antwerp, Belgium, 23rd September 2011.
  • Essone P, Kalsdorf B, Chegou NN, Loxton A, Kriel D, Schöllhorn V, Ernst M, Lange C, and Walzl G. Bifunctional T-cell derived cytokines allow for discrimination between latent and active tuberculosis. Invited talk presented at the ERS meeting during September 2011 in Amsterdam.
  • Warren RM. Characterizing Drug-Resistant Epidemics in South Africa. Invited talk presented at the Center for Communicable Disease Dynamics 2nd Annual Symposium. Boston, USA. 3-4 October 2011.
  • Walz G. Biomarkers of treatment response. Chronic Lung Disease Conference, Cape Town. November 2011.
  • Van Helden PD. Progress in TB vaccine development for humans and animals. Invited talk presented at the 30th World Veterinary Congress 2011. Cape Town International Convention Centre, Cape Town, South Africa. 10-14 October 2011.
  • Van Helden PD. Burden of disease from a medical perspective. Invited talk presented at the Coloquium on Zoonoses and neglected infectious diseases of Africa. Johannesburg, South Africa. 1-4 November 2011.
  • Van Helden PD. Diagnostic Development. Invited talk presented at the Coloquium on Zoonoses and neglected infectious diseases of Africa. Johannesburg, South Africa. 1
  • Van Helden PD. The African tuberculosis situation including Mycobacteria outside the TB complex species. Invited talk presented at the 46th Annual Meeting of The American Society for Veterinary Clinical Pathology. Nashville, Tennessee, USA, 3
  • Warren RM. Seminar presented on Drug-resistant TB for World TB Day. Youngsfield Military Base, Cape Town, 24 March 2011.
  • Warren RM. Emergence of Drug resistant Tuberculosis: A comedy of errors. Invited talk presented at the 4th FIDSSA Congress. Durban, South Africa, 8-11 September 2011.
  • Willemse D, Williams MJ, Victor TC. Regulation of efflux in rifampicin resistant mutants of Mycobacterium tuberculosis. Invited talk presented at the 55th Annual Faculty of Health Sciences Academic Year Day. Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Corfield V. Promoting women in science across cultures:  the contribution of science centers. Talk presented at the 6th Science Centre World Congress. Cape Town, South Africa, September 2011.
  • Corfield V. “Science and Society” be squeezed into a one-size-fits-all Science Centre experience? Talk presented at the 6th Science Centre World Congress. Cape Town, South Africa, September 2011.
  • Mapela L, Beukes G, Gordhan B, Kana B. Resuscitation promoting factors in bacterial growth, peptidoglycan remodelling and muropeptide production. Poster presentation. Gordon Research Conference on TB Drug Development. Il Ciocco Hotel and Resort, Lucca (Barga), Italy, 3-8 July.
  • Chengalroyen M, Kana B. The role of resuscitation promoting factor in the reactivation of Mycobacterium tuberculosis in sputum. Poster Presentation. MRC Research Day, MRC Conference Centre, Parow, Cape Town, 19-20 October.
  • Chengalroyen M, Kana B. The role of resuscitation promoting factor in the reactivation of Mycobacterium tuberculosis in sputum. Poster Presentation. Wits Molecular Biosciences Research Thrust Symposium, 7 December.
  • Williams M, Kana B, Mizrahi V. Analysis of molybdopterin cofactor biosynthetic genes in mycobateria. Poster presented at the Keystone Symposium on Mycobacteria: Physiology, Metabolism and Pathogenesis - Back to the Basics (J4), Vancouver, Canada, January 5-20, 2011
  • Ndwandwe DE, Venclovas C, Mizrahi V, Warner DF A novel mutasome in Mycobacterium tuberculosis. Poster presented at Keystone Symposiun on Mycobacteria: Physiology, Metabolism and Pathogenesis - Back to the Basics. Vancouver, Canada, January 15 - 20, 2011.
  • Ndwandwe DE, Venclovas C, Mizrahi, V, Warner, DF A novel mutasome in Mycobacterium tuberculosis. Poster presented at Fifth Annual New England TB Symposium on Vaccine Development: from Basic Research to Clinical Trials.  June 23, 2011. Broad Institute, Cambridge, USA.
  • Williams M, Kana BD, Mizrahi V. Phenotypic characterization of ∆mobA mutants of Mycobacterium tuberculosis and Mycobacterium smegmatis. Poster presented at the MRC Research Conference, Cape Town, 21 October 2011.
  • Moosa A, Mizrahi V, Warner D F. A Mycobacterium-specific protein family member required for vitamin B12 biosynthesis in M. tuberculosis. Poster presented at Keystone Symposiun on Mycobacteria: Physiology, Metabolism and Pathogenesis - Back to the Basics. Vancouver, Canada, January 15 - 20, 2011.
  • Koch A, Mizrahi V, Warner DF. The physiological implications of drug resistance mutations in mycobacteria. Poster presented at the 4th Postgraduate Research Day, Faculty of Health Sciences, University of Cape Town, 7 September 2011.
  • Naran K, Fenner C, Harrison STL, Mizrahi V, Warner DF. Characterization of the Antimycobacterial Effect of a Pseudomonas-Derived Activity. Poster presented at the 4th Postgraduate Research Day, Faculty of Health Sciences, University of Cape Town, 7 September 2011.
  • Naran K, Fenner C, Harrison STL, Mizrahi V, Warner DF. Characterization of the Antimycobacterial Effect of a Pseudomonas-Derived Activity. Poster presented at the South African Society of Microbiology (SASM) Conference, Cape Town, 6-9 November 2011.
  • Kleynhans L, Walzl G, Ronacher K. Medroxyprogesterone acetate alters mycobacterium bovis BCG induced cytokine expression in vitro and in PBMCS of contraceptive users. Poster presented at Tuberculosis:  Immunology, Cell Biology and Novel Vaccination Strategies (Keystone meeting), Faimont Hotel, Vancouver, British Columbia, Canada, 15-20 January 2011.
  • Macingwana L. Investigation of a synergistic effect of Sulfamethoxazole and Trimethoprim in combination with first-line TB drugs as potential first-line combination drug regime against Mycobacterium tuberculosis. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Victor T.  XDR-TB is evolving towards TDR-TB in the Eastern Cape, South Africa. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Gey van Pittius NC. A metabolomics approach to exploring the function of the ESX-3 type VII secretion system of M. smegmatis. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Louw GE. Rifampicin reduces susceptibility to ofloxacin in rifampicin resistant Mycobacterium tuberculosis through efflux. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Parsons S. Adaptation pf the QuantiFERON-TB gold (in tube) assay for the diagnoses of bovine tuberculosis in African buffaloes (Syncerus caffer). Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • van der Merwe R. The isolation, characterization and comparative genomics of novel South African mycobacteriophages from soil samples. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Wiid I. Elucidation of the physiological roles of Glutamine synthesas and Glutamate dehydrogenase in Mycobacterium smegmatis. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Svenson S. Differential activation of macrophages and dendritic cells by MDR and Non-MDR Mycobacterium tuberculosis strains. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Källenius G. Beijing Mycobacterium tuberculosis genotype is emerging in Mozambique in association with HIV co-infection. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Källenius G. Interferon inducible protein-10 (IP10) as a marker for Mycobacterium tuberculosis infection. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Källenius G. Mycobacterium tuberculosis strains causing the TB epidemic in Sweden a century ago. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Källenius G. A protective role for CD103+ lung dendritic cells during pulmonary tuberculosis.  Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Källenius G. Bystander effects of mycobacteria-infected macrophages on dendritic cells and their ability to mediate HIV-1 trans-infection. Poster presented at the 8th International Conference on the Pathogenesis of Mycobacterial Infections. Stockholm, Sweden, 30 June – 3 July 2011.
  • Möller M, Adams LA, Nebel A, Schreiber S, van der Merwe L, van Helden PD, Hoal EG. Polymorphisms in MC3R promoter and CTSZ 3`UTR are associated with tuberculosis susceptibility. Poster presented at the 12th International Congress of Human Genetics (ICHG) and the 61st ASHG Annual Meeting, Montréal, Canada, 11-15 October 2011.
  • Möller M, Quintana-Murci L, de Wit E, Delport W, Harmant C, Rugamika CE, Meintjes A, Balanovsky O, Zaporozhchenko V, Bormans C, van Helden PD, Seoighe C, Behar DM, Hoal EG. The History in our Genes: the Complex Structure of the South African Coloured Population. Poster presented at the Joint International Conference of the African and Southern Africa Societies of Human Genetics, Cape Town, South Africa, 6-9 March 2011.
  • Salie M, Möller M, Duh F-M, Martin MP, Hoal EG, Carrington M. The role of killer cell immunoglobulin-like receptors and their human leukocyte antigen class-I ligands in susceptibility to tuberculosis. Poster presented at the Joint International Conference of the African and Southern African Societies of Human Genetics. Cape Town, South Africa, 6-9 March 2011.
  • Le Roex N. Solid SNPs in Syncerus: Novel SNP detection in African buffalo. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Ehlers L, Chegou NN, Walzl G, van Helden PD, Ronacher K. Effect of TB Treatment on Metabolic Hormone Profiles. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Möller M, Adams LA, Nebel A, Schreiber S, van der Merwe L, van Helden PD, Hoal EG. Polymorphisms in MC3R promoter and CTSZ 3`UTR are associated with tuberculosis susceptibility. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Siame KK. A phylogenomic and Proteomic Investigation into the Evolution and Biological Characteristics of the Principal Genetic Group 1 Members of Mycobacterium tuberculosis. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Steyn NL. Stellenbosch University, South Africa. Investigating the localization of ESX-3 secretion system components in Mycobacterium smegmatis. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Van der Merwe C. An investigation into the role of mitochondrial dysfunction in Parkinson's disease. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Wagman C, Möller M, van der Merwe L, Hoal EG. Investigating single nucleotide polymorphisms and interactions of candidate genes involved in susceptibility to tuberculosis in a South African population. Poster presented at the 55th Annual Faculty of Health Sciences Academic Year Day, Stellenbosch University, Cape Town, South Africa, 17-18 August 2011.
  • Corfield V. Catalysing partnerships: can science centres bridge the gap and promote dialogue between scientists and the public in biomedicine? Poster presented at the 6th Science Centre World Congress. Cape Town, South Africa, September 2011.
  • Corfield V. SA WISE: South African sisters in science and engineering network with science centres. Poster presented at the 6th Science Centre World Congress. Cape Town, South Africa, September 2011.
  • Ehlers L. Effect of TB Treatment on Metabolic Hormone Profiles. Poster presented at the Medical Research Council Research Day. Cape Town, South Africa, 19-20 October 2011.
  • Willemse D, Victor TC, Williams MJ. Regulation of efflux in rifampicin resistant mutants of Mycobacterium tuberculosis. Poster presented at the Medical Research Council Research Day. Cape Town, South Africa, 19-20 October 2011.

Other Relevant Outputs (including honours and awards to staff)

  1. In October 2011, Dr. Bavesh Kana from the Wits node was invited to give the opening plenary lecture at the MRC Research Day, held at the MRC Conference Centre in Parrow. Dr. Kana’s lecture was attended by all participants of the Research Day, the acting president of the MRC and some members from the MRC Board. Dr. Kana was invited on the basis of being an MRC Career Awardee.
  2. Dr. Kana from the Wits node received a renewal for his MRC Career Development Award. This was based on a competitive renewal process for this program and provides a further two years of funding to the Wits node.
  3. Dr. Bhavna Gordhan from the Wits node was selected to give oral presentations at two local conferences/meetings as testimony to the high quality of their research.
  4. Prof. Mizrahi was invited to London for an interview under the auspices of the Exchanges at the Frontier program of the UK-based Wellcome Collection. The interview, which took place at the Wellcome Collection on 21 October 2011. An abridged version of the interview was broadcast by BBC World on 9 Dec 2011 to an estimated audience of 40-50 million listeners worldwide. The podcast is available online and can be downloaded from http://www.bbc.co.uk/podcasts/series/discovery
  5. Dr. Digby Warner was the overall winner of the prestigious 2011 BioVision-Lilly Award in conjunction with the Academy of Sciences for the Developing World (TWAS). The award recognises young researchers from developing countries for outstanding scientific achievements in tuberculosis-related research. The winners were announced at a special ceremony at the 7th Biovision World Life Sciences Forum
  6. Dr. Digby Warner awarded a K-RITH Collaborative Travel Award.
  7. Prof. Mizrahi was appointed to the Editorial Board of the international journal, Emerging Microbes & Infection
  8. Dr. Digby Warner was appointed as an Associate Member of the IIDMM
  9. Gey van Pittius, NC was elected as a member of the Academy of Science of South Africa (ASSAf)
  10. Hoal, EG. Obtained a B2 rating by the NRF (2011)
  11. Walzl G. Invited to the AERAS vaccine research workshop
  12. Walzl G. Workgroup member of Endpoints and Biomarker Working Groups of the Critical Path to New TB Drug Regimens
  13. Walzl G. A member of the core writing team of the International Roadmap for Tuberculosis Research
2010

Books / Chapters in Books (Total: 2)

  • McEvoy CRE, Warren RM, van Helden PD. 2010. Molecular Methods and Their Application in TB Epidemiology. In: Schaaf HS, Zumla AI (ed.) Tuberculosis. A Comprehensive Clinical Reference. Elsevier Publications. pp 28 – 37.
  • Dheda K, Warren RM, Zumla A, Grobusch MP. 2010. Extensively drug-resistant tuberculosis: epidemiology and management challenges. Infect Dis Clin North Am 24:705-725.

Articles in Peer-Reviewed Journals (Total: 46)

  • Warner DF, Ndwandwe DE, Abrahams GL, Kana BD, Machowski EE, Venclovas C, Mizrahi V. (2010) Essential roles for imuA'- and imuB-encoded accessory factors in DnaE2-dependent mutagenesis in Mycobacterium tuberculosis. Proc Natl Acad Sci USA. 107(29):13093-13098. (IF=10.312)
  • Kana BD, Mizrahi V, Gordhan BG. (2010) Depletion of resuscitation-promoting factors has limited impact on the drug susceptibility of Mycobacterium tuberculosis. J Antimicrob Agents Chemother. 65(8):1583-1585 (IF=4.294)
  • Ioerger TR, Feng Y, Ganesula K, Chen X, Dobos KM, Fortune S, Jacobs WR Jr, Mizrahi V, Parish T, Rubin E, Sassetti C, Sacchettini JC. (2010) Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories. J Bacteriol. 192(14):3645-3653. (IF=4.035)
  • Kana BD, Abrahams GL, Sung N, Warner DF, Gordhan BG, Machowski EE, Tsenova L, Sacchettini JC, Stoker NG, Kaplan G, Mizrahi V. (2010) Role of the DinB homologs Rv1537 and Rv3056 in Mycobacterium tuberculosis. J Bacteriol. 192(8):2220-2227. (IF=4.035)
  • Capron M, Mizrahi V. (2010) Highlights from the first meeting of the Europe-Africa frontier research conference series infectious diseases: from basic to translational research. FEMS Immunol Med Microbiol. 58(1):1-2. (IF=2.352)
  • Kana BD, Mizrahi V. (2010) Resuscitation-promoting factors as lytic enzymes for bacterial growth and signalling. FEMS Immunol Med Microbiol. 58(1):39-50. (IF=2.352)
  • Aguilar D, Hanekom M, Mata D, Gey van Pittius NC, van Helden PD, Warren RM, Hernandez-Pando R. (2010) Mycobacterium tuberculosis strains with the Beijing genotype demonstrate variability in virulence associated with transmission. Tuberculosis (Edinb) 90:319-25. (IF=2.986)
  • Alexander KA, Laver PN, Michel AL, Williams M, van Helden PD, Warren RM, Gey van Pittius NC. (2010) Novel Mycobacterium tuberculosis complex pathogen, M. mungi. Emerg Infect Dis 16:1296-9. (IF=6.497)
  • Calver AD, Falmer AA, Murray M, Strauss OJ, Streicher EM, Hanekom M, Liversage T, Masibi M, van Helden PD, Warren RM, Victor TC. (2010) Emergence of increased resistance and extensively drug-resistant tuberculosis despite treatment adherence, South Africa. Emerg Infect Dis 16:264-271. (IF=6.497)
  • Cilliers K, Labadarios D, Schaaf HS, Willemse M, Maritz JS, Werely CJ, Hussey G, Donald PR. (2010) Pyridoxal-5-phosphate plasma concentrations in children receiving tuberculosis chemotherapy including isoniazid. Acta Paediatr. 99(5):705-710 (IF=1.995)
  • Cobat A, Gallant CJ, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Hoal EG, Alcaïs A, Schurr E. (2010) High heritability of antimycobacterial immunity in an area of hyperendemicity for tuberculosis disease. J Infect Dis 201:15-9. (IF=5.698)
  • De Souza GA, Fortuin S, Aguilar D, Hernandez Pando R, McEvoy CRE, van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG (2010). Using a label-free proteomic method to identify differentially abundant proteins in closely related hypo- and hyper-virulent clinical Mycobacterium tuberculosis Beijing isolates. Mol. Cell Proteomics. 9: 2414-2423. (IF=9.606)
  • Demers A-M, Boulle A, Warren RM, Verver S, van Helden PD, Behr MA, Coetzee D. (2010) Use of simulated sputum specimens to estimate the specificity of laboratory-diagnosed tuberculosis. Int J Tuberc Lung Dis 14:1016-23. (IF=2.442)
  • Demers A-M, Mostowy S, Coetzee D, Warren RM, van Helden PD, Behr MA. (2010) Mycobacterium africanum is not a major cause of human tuberculosis in Cape Town, South Africa. Tuberculosis (Edinb) 90:143-4. (IF=2.986)
  • Dheda K, Shean K, Zumla A, Badri M, Streicher EM, Page-Shipp L, Willcox P, John M-A, Reubenson G, Govindasamy D, Wong M, Padanilam X, Dziwiecki A, van Helden PD, Siwendu S, Jarand J, Menezes CN, Burns A, Victor TC, Warren RM, Grobusch MP, van der Walt M, Kvasnovsky C. (2010) Early treatment outcomes and HIV status of patients with extensively drug-resistant tuberculosis in South Africa: a retrospective cohort study. Lancet 375:1798-807. (IF=29.443)
  • Dheda K, Warren RM, Zumla A, Grobusch MP. 2010. Extensively drug-resistant tuberculosis: epidemiology and management challenges. Infect Dis Clin North Am 24:705-725. (IF=2.290)
  • Diacon AH, Dawson R, Hanekom M, Narunsky K, Maritz SJ, Venter A, Donald PR, Van Niekerk C, Whitney K, Rouse DJ, Laurenzi MW, Ginsberg AM, Spigelman MK. (2010) Early bactericidal activity and pharmacokinetics of PA-824 in smear-positive Tuberculosis patients. Antimicrob Agents Chemother. 54(8): 3402-3407. (IF=4.294)
  • Diacon AH, Maritz JS, Venter A, Van Helden PD, Andries K, McNeeley DF, Donald PR. (2010) Time to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents. Eur J Clin Microbiol Infect Dis. 29 (12): 1561-5. (IF=2.560)
  • Gallant CJ, Cobat A, Hoal EG, Schurr E. (2010) Quantifying latent TB infection: The dilemma continues. Chest. 138: 460-461. (IF=5.442)
  • Gallant CJ, Cobat A, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Alcaïs A, Hoal EG, Schurr E. (2010) Impact of age and sex on mycobacterial immunity in an area of high tuberculosis incidence. Int J Tuberc Lung Dis 14:952-9. (IF=2.442)
  • Gallant CJ, Cobat A, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Alcaïs A, Hoal EG, Schurr E. (2010) Tuberculin skin test and in vitro assays provide complementary measures of antimycobacterial immunity in children and adolescents. Chest 137:1071-7. (IF=5.442)
  • Govender L, Abel B, Hughes EJ, Scriba TJ, Kagina BM, de Kock M, Walzl G, Black G, Rosenkrands I, Hussey GD, Mahomed H, Andersen P, Hanekom WA. (2010) Higher human CD4 T cell response to novel Mycobacterium tuberculosis latency associated antigens Rv2660 and Rv2659 in latent infection compared with tuberculosis disease. Vaccine 29(1): 51-57. (IF=3.508)
  • Harper CJ, Hayward D, Kidd M, Wiid I, van Helden PD. (2010) Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis. BMC Microbiol 10:138. (IF=3.329)
  • Hesseling AC, Marais BJ, Kirchner HL, Mandalakas AM, Brittle W, Victor TC, Warren RM, Schaaf HS. (2010) Mycobacterial genotype is associated with disease phenotype in children. Int J Tuberc Lung Dis 14:1252-8. (IF=2.442)
  • Hesseling AC, Walzl G, Enarson DA, Carroll NM, Duncan K, Lukey PT, Lombard C, Donald PR, Lawrence KA, Gie RP, van Helden PD, Beyers N. (2010) Baseline sputum time to detection predicts month two culture conversion and relapse in non-HIV-infected patients. Int J Tuberc Lung Dis 14:560-70. (IF=2.442)
  • Human H, Hagen CM, de Jong G, Harris T, Lombard D, Christiansen M, Bardien S. (2010) Investigation of mitochondrial sequence variants associated with aminoglycoside-induced ototoxicity in South African TB patients on aminoglycosides. Biochem Biophys Res Commun 393:751-6. (IF=2.270)
  • Innes S, Schaaf HS, Hoek KGP, Rabie H, Cotton MF. (2010) Unsuspected fatal drug-resistant tuberculosis in a closely monitored child: a plea for improved source-case tracing and drug susceptibility testing. South Afr J Epidemiol Infect. 25(2): 30-32. (IF not available)
  • Ioerger TR, Feng Y, Chen X, Dobos KM, Victor TC, Streicher EM, Warren RM, Gey van Pittius NC, Van Helden PD, Sacchettini JC. (2010) The Non-clonality of Drug Resistance in Beijing-genotype Isolates of Mycobacterium tuberculosis from the Western Cape of South Africa. BMC Genomics. 11: 670. (IF=4.179)
  • Johnson R, Warren RM, van der Spuy GD, Gey van Pittius NC, Theron D, Streicher EM, Bosman M, Coetzee GJ, van Helden PD, Victor TC. (2010) Drug-resistant tuberculosis epidemic in the Western Cape driven by a virulent Beijing genotype strain. Int J Tuberc Lung Dis 14:119-21. (IF=2.442)
  • Koegelenberg CFN, Bolliger CT, Theron J, Walzl G, Wright CA, Louw M, Diacon AH. (2010) Direct comparison of the diagnostic yield of ultrasound-assisted Abrams and Tru-Cut needle biopsies for pleural tuberculosis. Thorax 65:857-62. (IF=6.906)
  • Mallard K, McNerney R, Crampin AC, Houben R, Ndlovu R, Munthali L, Warren RM, French N, Glynn JR. (2010) Molecular detection of mixed infections of Mycobacterium tuberculosis in sputum samples from patients in Karonga District, Malawi. J Clin Microbiol. 48 (12): 4512-4518. (IF=4.126)
  • Michel AL, Müller B, van Helden PD. (2010) Mycobacterium bovis at the animal-human interface: a problem, or not? Vet Microbiol 140:371-381.
  • Möller M, de Wit E, Hoal EG. (2010) Past, present and future directions in human genetic susceptibility to tuberculosis. FEMS Immunol Med Microbiol 58:3-26. (IF=2.352)
  • Möller M, Flachsbart F, Till A, Thye T, Horstmann RD, Meyer CG, Osei I, van Helden PD, Hoal EG, Schreiber S, Nebel A, Franke A. (2010) A functional haplotype in the 3'untranslated region of TNFRSF1B is associated with tuberculosis in two African populations. Am J Respir Crit Care Med 181:388-93. (IF=9.881)
  • Möller M, Hoal EG. (2010) Current findings, challenges and novel approaches in human genetic susceptibility to tuberculosis. Tuberculosis (Edinb) 90:71-83. (IF=2.986)
  • Möller M, Nebel A, van Helden PD, Schreiber S, Hoal EG. (2010) Analysis of eight genes modulating interferon gamma and human genetic susceptibility to tuberculosis: a case-control association study. BMC Infect Dis 10:154. (IF=2.715)
  • Muyoyeta M, de Haas PEW, Mueller DH, van Helden PD, Mwenge L, Schaap A, Kruger C, Gey van Pittius NC, Lawrence K, Beyers N, Godfrey-Faussett P, Ayles H. (2010) Evaluation of the Capilia TB assay for culture confirmation of Mycobacterium tuberculosis infections in Zambia and South Africa. J Clin Microbiol 48:3773-5. (IF=4.126)
  • Onajole OK, Govender P, van Helden PD, Kruger HG, Maguire GEM, Wiid I,  Govender T. (2010) Synthesis and evaluation of SQ109 analogues as potential anti-tuberculosis candidates. Eur J Med Chem 45:2075-9. (IF=3.363)
  • Parsons SDC, de Villiers C, Gey van Pittius NC, Warren RM, van Helden PD. (2010) Detection of Mycobacterium kansasii infection in a rhesus macaque (Macaca mulatta) using a modified QuantiFERON-TB Gold assay. Vet Immunol Immunopathol 136:330-4. (IF=2.211)
  • Repsilber D, Kern S, Telaar A, Walzl G, Black GF, Selbig J, Parida SK, Kaufmann SHE, Jacobsen M. (2010) Biomarker discovery in heterogeneous tissue samples -taking the in-silico deconfounding approach. BMC Bioinformatics 11:27. (IF=4.108)
  • Roberts L, Passmore JS, Williamson C, Little F, Bebell LM, Mlisanac K, Burgers WA, van Loggerenberg F, Walzl G, Djoba Siawaya JF, Abdool Karim Q and Abdool Karim SS. (2010) Plasma cytokine levels during acute HIV-1 infection predict HIV disease progression. AIDS  24(6):819–831. (IF=5.334)
  • Sester M, Giehl C, McNerney R, Kampmann B, Walzl G, Cuchí P, Wingfield C, Lange C, Migliori GB, Kritski AL, Meyerhans A; European Network for global cooperation in the field of AIDS and TB (EUCO-Net). (2010) Challenges and perspectives for improved management of HIV/Mycobacterium tuberculosis co-infection. Eur Respir J. 36(6):1242-1247.  (IF=5.076)
  • Stefan DC, Dippenaar A, Detjen AK, Schaaf HS, Marais BJ, Kriel B, Loebenberg L, Walzl G, Hesseling AC. (2010) Interferon-gamma release assays for the detection of Mycobacterium tuberculosis infection in children with cancer. Int J Tuberc Lung Dis. 14(6): 689-694. (IF=2.442)
  • Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, Zumla A. (2010) Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet 375:1920-37. (IF=29.443)
    Wright CA, Bamford C, Prince Y, Vermaak A, Hoek KGP, Marais BJ, Warren RM. (2010) Mycobacterial transport medium for routine culture of fine needle aspiration biopsies. Arch Dis Child 95:48-50. (IF=2.620)
  • Wright CA, Hoek KGP, Marais BJ, van Helden PD, Warren RM. (2010) Combining fine-needle aspiration biopsy (FNAB) and high-resolution melt analysis to reduce diagnostic delay in Mycobacterial lymphadenitis. Diagn Cytopathol 38:482-8. (IF=1.215)

Non Peer-Reviewed Articles (Total: 3)

  • Marais BJ, van Helden PD, Donald PR. (2010) Adequacy of 6-month follow-up in chemotherapy trials. Int J Tuberc Lung Dis 14:927; author reply 928. (IF=2.442)
  • Van Helden PD. (2010) Is bigger better? EMBO Reports 11:408.
  • Van Helden PD. (2010) Managing Academic Research. EMBO Reports 11:648.

Published Abstracts (Total: 0)

Technical Reports (Total: 0)

Products / Artefacts / Patents (Total: 0)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 89)

  • Abrahams GL, Wei H, Ciulli A, Sherman DR, Abell C, Barry CE III, Boshoff HIM,  Mizrahi V. New tools for tuberculosis drug discovery: Development of novel screening strains of M. tuberculosis and application in target-based approaches. Plenary lecture presented at the Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, NM, 14-19 February 2010.
  • Wei AH, Silvestre HL, Wen S, Abrahams GL, Sherman DR, Barry CE III, Boshoff HIM, Ciulli A, Blundell TL, Abell C. Application of fragment-based approaches to the discovery of new agents against Mycobacterium tuberculosis. Plenary lecture presented at the  Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, New Mexico, 13-19 February, 2010
  • Mizrahi V. Early-stage drug discovery. Plenary lecture presented at the NIH/HHMI/SA MRC US-Southern Africa Joint Research Forum on Tuberculosis, Pretoria, 1-2 March 2010.
  • Mizrahi V. Summary of the NIH/HHMI/SA MRC US-Southern Africa Joint Research Forum on Tuberculosis. Talk presented at the Research Forum (2 March 2010), and at the following Institute of Medicine- Academy of Science of South Africa Workshop on the Emerging threat of drug-resistant tuberculosis: Global and local challenges and solutions, Pretoria, 3-4 March 2010.
  • Abrahams GL, Mizrahi V. Target-based whole-cell screening. Talk presented at the IMTB Annual Meeting, Martha’s Vineyard, MA, 5-6 May 2010.
  • Mizrahi V. Taking advantage of stress: DNA damage, mutagenesis and the evolution of drug resistance in M. tuberculosis. Division U Plenary Lecture, presented at the 110th General Meeting of the American Society for Microbiology, San Diego, CA. 24-28 May 2010.
  • Cholo MC, Cockeran, R., Mizrahi, V., van Rensburg, E.J., Stoker, N.G., and Anderson, R. Evaluation of the Relative Efficiencies and Preferential Utilization at Various Stages During Growth of the Trk and Kdp Potassium Transporters of Mycobacterium Tuberculosis. Talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Koch A, Mizrahi V, Warner DF. The fitness cost of drug resistance mutations in mycobacteria. Invited talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Gordhan BG. The role of DNA glycosylases in mutagenesis and adaptation to stress in mycobacteria. Invited talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Kana BD.  From TB Pathogenesis to Drug Development. Plenary lecture presented at the 2nd SA TB Conference, Durban, 2-5 June 2010
  • Warner DF.  A novel mechanism of damage-induced mutagenesis in Mycobacterium tuberculosis. Invited talk presented to the National TB Reference Laboratory, National Institute for Communicable Diseases, Johannesburg, 19 July 2010.
  • Warner DF. Taking advantage of stress: DNA damage-induced mutagenesis in Mycobacterium tuberculosis.  Invited talk presented to a visiting delegation led by Prof. Ronald Atlas under the People To People Citizen Ambassador Programme, University of the Witwatersrand, 21 July 2010.
  • Mizrahi V. TB Drug Discovery Challenges from a Mycobacterial Physiology Perspective. Invited talk presented at the Scientific Advisory Committee meeting of the Global Alliance for TB Drug Development, New York, 28 July 2010.
  • Mizrahi V. Taking Advantage of Stress: DNA damage, mutagenesis and the evolution of drug resistance in M. tuberculosis. Plenary Lecture delivered at the UKZN College of Health Sciences Research Symposium, Durban, 15 September 2010.
  • Ahidjo BA, Gordhan BG, Mizrahi V. Nuclease-like VapC toxins from mycobacterium tuberculosis differentially inhibit mycobacterial growth and are specifically neutralised by their cognate antitoxins. Invited talk presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010. [Winner, Best Young Researcher Award, Infectious Diseases category]
  • Koch A, Mizrahi V, Warner DF. The fitness cost of rifampicin resistance in mycobacteria. Invited talk presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Koch A, Mizrahi V, Warner DF. The physiological implications of drug resistance in Mycobacteria. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Ndwandwe DE, Mizrahi V, Warner DF. A novel mutasome in Mycobacterium tuberculosis. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Moosa A, Mizrahi V, Warner DF. The metabolism of vitamin B12 in Mycobacterium tuberculosis: role of alternate B12 cofactors. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Naran K, Veale R, Mizrahi V, Warner DF. Characterisation of the anti-mycobacterial effect of a Pseudomonas-derived activity. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Abrahams GL, Wei H, Ciulli A, Sherman DR. Abell C, Barry CE III, Boshoff HIM,  Mizrahi V. New tools for tuberculosis drug discovery: Development of novel screening strains of M. tuberculosis and application in target-based approaches. Poster lecture presented at the Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, NM, 14-19 February 2010.
  • Kana BD, Mizrahi V, Gordhan, BG. The role of resuscitation promoting factors in drug susceptibility and stress adaptation of Mycobacterium tuberculosis. Poster presented at the Gordon Research Conference on Bacterial Cell Surfaces, Colby Sawyer College, NH, USA, 27 June – 2 July 2010.
  • Koch A, Mizrahi V, Warner DF. The fitness cost of rifampicin resistance in mycobacteria. Poster presented at the FEBS-EMBO Lecture Course on Host-Microbe Interactions, Spetses, Greece, 3-10 September 2010. [Winner, Best Poster Prize]
  • Naran K, Mizrahi V, Warner DF. A Pseudomonas-derived extract that inhibits growth of Mycobacterium tuberculosis Poster presented at the FEBS-EMBO Lecture Course on Host-Microbe Interactions, Spetses, Greece, 3-10 September 2010.
  • Moosa A, Mizrahi V, Warner DF. A role for a mycobacterium-specific protein family in vitamin B12 biosynthesis in Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010. [Winner, Best Poster Prize, Infectious Diseases category]
  • Naran K, Mizrahi V, Warner DF. A Pseudomonas-derived extract that inhibits growth of Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Narandes N, Mizrahi V, Kana BD. Functional characterisation of molybdopterin synthase-encoding genes in mycobacteria. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Ndwandwe DE, Mizrahi V, Warner DF. A novel mutasome in Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Ahmadou Ahidjo B, Kuhnert D, Machowski EE, Gordhan BG, Abrahams GL, Mizrahi V. VapC toxins from Mycobacterium tuberculosis differentially inhibit mycobacterial growth and are specifically neutralised by their cognate antitoxins. Poster presented at the NITD Symposium on Tuberculosis 2010, Yaounde, Cameroon, 11-15 October 2010.
  • Mapela L, Kana BD. Characterisation of resuscitation promoting factors in M. smegmatis. Poster presented at the MRC Research Day, Cape Town, 14 October 2010.
  • Narrandes N, Mizrahi V, Kana BD. Functional characterization of molybdopterin synthase-encoding genes in mycobacteria. Poster presented at the MRC Research Day, Cape Town, 14 October 2010.
  • Lucas LA, Möller M, Nebel A, Van Helden PD, Schreiber S, Hoal EG. The role of Toll like receptors in genetic host susceptibility to tuberculosis. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Muleya V. Structural Characterisation of the Interaction Between RBBP6 and the Multifunctional Protein YB-1.  Talk presented at the 10th Astrazeneca Health Sciences Research Day. University of Stellenbosch, 25 February 2010.
  • Ngombane N, Ottenhoff THM, Kidd M, Walzl G, Black G. The predictive role of cytokine expression patterns of recently exposed tuberculin skin test negative TB contacts in the Western Cape Province. Talk presented at the 10th AstraZeneca Health Sciences Research Day, University of Stellenbosch, South Africa, 25 February 2010.
  • Salie M, Möller M, Van Helden PD, Schreiber S, Hoal EG, Nebel A, Hofmann S. A 3’UTR polymorphism in ANXA11 is associated with pulmonary tuberculosis in the South African coloured population. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Wagman C, Möller M, Hoal EG. Marco haplotypes and susceptibility to tuberculosis. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Warren RM. Molecular and Genetic epidemiology of TB in South Africa. Invited talk presented at the US- Southern Africa Joint research forum on Tuberculosis. Roode Valley Country Lodge, Pretoria, South Africa, 1-2 March 2010.
  • Warren RM; Genetic analysis of drug resistant strains in South Africa. Invited stalk at the ASSAF meeting on MDR-TB, Pretoria, 3-4 March 2010.
  • De Vos M. Rifampicin induces differential protein expression in Mycobacterium tuberculosis. Invited talk at the 2nd TB Conference on forging strategic partnerships to Fight TB and HIV. Durban, South Africa, 2-3 June 2010.
  • Van Helden PD. Quantitative first- and second-line drug susceptibility testing (DST) on MGIT 960 using BD EpiCenter Software. Invited talk presented at the 2nd TB Conference on Forging strategic partnerships to Fight TB and HIV. Durban, South Africa, 2-3 June 2010.
  • Black PA, Warren RM, Louw GE, Van Helden PD, Victor TC. Identification of novel mechanisms of drug resistance in Mycobacteria byTransposon Mutagenesis. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • De Vos M, Warren RM, Johnson R, Van Helden PD, Ndimba BK, Victor RC. Rifampicin induces differential protein expression in Mycobacterium Tuberculosis. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Hoal EG.  The History in our Genes: the Complex Structure of the South African Coloured Population.  Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Macingwana L, Baker B, Ngwane A, Van Helden PD, Cotton M, Wiid IJF. Investigation of synergistic effect of Isoniazid, Sulfamethoxazole and Trimethoprim as potential first-line combination drug regimen against Mtb. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Streicher EM, Gey Van Pittius NC, De Kock M, Van Helden PD, Victor TC, Warren RM. Acquisition of resistance to anti-Tuberculosis drugs may compromise the utility of genetic based drug resistance tests. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Tait M, Sirgel F, Trollip A, Warren RM, Coetzee G, Victor TC. Cross-resistance compromises the use of Capreomycin in the treatment of XDR-TB. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Hoek KGP, Wright CA, Marais BJ, Van Helden PD, Warren RM. High-resolution melt analysis for the detection of Mycobacterial Lymphadenitis using Whatman Fta® Elute cards. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Warren RM. Recent findings in antibiotic resistance in TB. Joint IPCAN/IFIC. Invited talk presented at Spier, Stellenbosch, South Africa, 29 August - 1 September 2010.
  • Warren RM. Molecular Epidemiology and the Genesis of Drug-Resistant TB. Talk presented at the Workshop on Drug-Resistant TB: Current Practise, Controversies, and Clinical Challenges. University of Cape Town, Cape Town, South Africa, 3-5 September 2010.
  • Diacon AH. Emerging drugs for the management of drug-resistant TB. Talk presented at the Workshop on Drug-Resistant TB: Current Practise, Controversies, and Clinical Challenges. University of Cape Town, Cape Town, South Africa, 3-5 September 2010.
  • Ronacher K, Kleynhans L, Ehlers L, Walzl G. Medroxyprogesterone-acetate alters cytokine expression in response to mycobacterium bovis BCG in vitro and in PBMCs of contraceptive users. Talk presented at the SATBAT Meeting, Johannesburg, South Africa, 12 October 2010.
  • Ronacher K, Kleynhans L, Ehlers L, Walzl G. Medroxyprogesterone-acetate alters cytokine expression in response to mycobacterium bovis BCG in vitro and in PBMCs of contraceptive users. Talk presented at the South African Immunology Society Conference. Lanseria, South Africa, 5-8 Dec 2010.
  • Walzl G, Chegou NN, Loxton A, Ronacher K. Biomarkers for different forms of tuberculosis. Talk presented at the South African Immunology Society Conference. Lanseria, South Africa, 5-8 December 2010.
  • De Souza GA, Fortuin S, Aguilar D, Hernandez-Pando R, McEvoy CRE, Van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG. Using a label-free proteomic method to identify differentially abundant proteins in closely related hypo and hyper-virulent clinical Mycobacterium tuberculosis Beijing isolates. Talk presented at the Analitika International Conference on Analytical Science, Stellenbosch, South Africa, 5 - 9 December 2010.
  • Gey van Pittius NC. Tuberculosis in South Africa- problem setting - epidemiology data and current trends. Invited talk presented at Koch-Metschnikow Forum Fourth Scientific Symposium on the occasion of World Tuberculosis Day 2010. Berlin, Germany, March 22-23 2010.
  • Warren RM, Gey van Pittius NC, Streicher EM, Louw GE, Calver A, Hernando-Pandez R, De Vos M, Tait M, Trollip A, Chiota V, Hanekom M, Coetzee G, Van der Spuy G, Muller B, Victor TC, Van Helden PD. Mycobacterium tuberculosis adaptation: answers or questions. Invited talk presented at the TB Adapt meeting. Rio De Janeiro, Brazil, 25-26 May 2010.
  • Warren RM, Gey van Pittius NC, Streicher EM, Louw GE, Calver A, Hernando-Pandez R, De Vos M, Tait M, Trollip A, Chiota V, Hanekom M, Coetzee G, Van der Spuy G, Muller B, Victor TC van Helden PD. A compilation of recent findings in molecular epidemiology and diagnostics of tuberculosis in South Africa. Talk presented at the IV National Tuberculosis Meeting/ 1st Brazilian STOP TB Partners’ Forum. Rio De Janeiro, Brazil, 26 - 29 May 2010.
  • Gey van Pittius NC. Tuberculosis in South Africa - from a treatable disease to total drug resistance. Invited talk presented at the Institute of Medicine (IOM) of the USA and the Russian Academy of Medical Sciences (RAMS) Forum on Drug Discovery, Development, and Translation. Moscow, Russia, 26-27 May 2010.
  • Victor TC, Streicher EM, Tait M, Johnson R, Van der Spuy GD, Gey van Pittius NC, Theron D, Bosman M, Coetzee GJ, Van Helden PD, Chabula-Nxiweni EM, Trollip A, Warren RM. Molecular insights Into the XDR-TB Epidemic in the Western And Eastern Cape Regions of South Africa. Invited talk presented at the ASM conference. San Diego, USA, 23-27 May 2010.
  • Louw GE, Warren RM, Gey van Pittius NC, McEvoy CRE, Murray M, Van Helden PD, Victor TC. Activated efflux pumps define the level of Rifampicin resistance in M. tuberculosis. Invited talk presented at the ASM Conference, San Diego, USA, 23-27 May 2010.
  • Victor TC. Molecular insights Into the XDR-TB Epidemic in the Western and Eastern Cape Regions of South Africa. Invited speaker at the Global Forum for infectious diseases. University California, San Diego, USA, 23-27 May 2010.
  • Van Helden. Susceptibility testing of Mycobacterium tuberculosis: The why and how in the developing world. Invited talk presented at the 50th Interscience conference on antimicrobial agents and chemotherapy. Boston, USA, 12-15 September 2010.
  • Victor TC. Insights into MDR- and XDR-TB in South Africa. Invited talk presented at a meeting to discuss the application of genetics- and genomics-based tools for diagnosis, drug susceptibility testing and epidemiology. Tunis, Tunisia, 20-22 September 2010.
  • Diacon AH. TMC-207 versus placebo plus OBT for the treatment of MDR-TB: a prospective clinical trial. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Van Helden PD. Microbial genetic determinants of TB infection and disease progression. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Walzl G. Biomarkers for use in clinical trials. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Stead M. Frequency and clinical outcome of mixed infections in South African TB patients. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • De Vos M, Warren RM, Johnson R, Van Helden PD, Ndimba BK, Victor TC. RIF induces diferential protein expression in Mycobacterium tuberculosis. Poster presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Ngwane A, Wiid IJF, Van Helden PD. Elucidation of the mechanism of action of a novel furanone based antituberculosis compound (F1082). Poster presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Fortuin S. A label-free method identified differentially abundant proteins in related M. tuberculosis Beijing strains, Poster presented at the ESF 4th conference on Functional Genomics and Disease, Dresden, Germany, 14–17 April 2010.
  • Bruiners N, Gey Van Pittius NC, Warren RM. Investigation Of Complement C1q Polymorphisms In A Cohort Of Tuberculosis Infected Patients. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Dramowski A, Morsheimer MM, Jordaan A, Victor TC, Donald PR, Schaaf HS. Rifampicin Mono-Resistant Mycobacterium Tuberculosis Disease: An Increasing Trend Among Children In Cape Town. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Fang Z, Gey Van Pittius NC, Van Helden PD. Elucidation Of The Substrates Of Mycosin 3, An Essential Protease of M.Tuberculosis. Poster presented at the 54th Academic Year Day, University Of Stellenbosch, South Africa, 11-12 August 2010.
  • Friedrich SO, Nabeta P, Boehme C, Venter A, Diacon AH.  Direct comparison of GeneXpert with smear microscopy, solid and liquid culture for sputum monitoring of tuberculosis (TB) patients. Poster presented at the  54th Annual Academic Day, Stellenbosch University, South Africa, 11-12 August 2010.
  • Grobbelaar M, Louw GE, Warren RM, Gey Van Pittius NC, Van Helden PD, Victor TC. New hope for treatment of drug resistant Tuberculosis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Kirsten CJ, Hayward D, Wiid IJF, Van Helden PD. Identification of the Transcriptional Regulator of Nitrogen Related Genes In Mycobacterium Smegmatis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Lucas LA, Möller M, Nebel A, Van Helden PD, Schreiber S, Hoal EG. Genetic Variants of Toll- Like Receptors 2 & 8 And Their Possible Role In Susceptibility To Pulmonary Tuberculosis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Newton-Foot M, Warren RM, Van Helden PD, Gey Van Pittius NC. The Mycobacterium Tuberculosis Esx-3 Secretion System Interactome. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Phalane KG, Esterhuyse MM, Hoal EG. Evaluation of Differential Dna Methylation Levels In The Interleukin 8 (Il8) Gene Of Tuberculosis Infected Cases And Controls. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Seepe PM, Warren RM, Johnson R, Van Helden PD, Louw GE, Victor TC. Differential Expression Of Genes In Clinical Strains of Mycobacterium Tuberculosis In Response to Isoniazid. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Siame KK, Gey Van Pittius NC, Warren RM. Investigating the Genome Variation and Evolution of Group 1 M. Tuberculosis Strains. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Steyn NL, Smit M, Newton-Foot M, Gey Van Pittius NC. The Construction of Genetic Knock-Outs of the Esx Secretion Systems In Mycobacterium Smegmatis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Viljoen AJ, Kirsten C, Hayward D, Wiid IJF, Van Helden PD. Regulation of the Nitrogen Assimilatory Enzymes, Glutamine Synthetase And Glutamate Dehydrogenase In Mycobacterium Smegmatis And M. Bovis BCG. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Seepe PM, Johnson R, Louw GE, Van Helden PD, Warren RM, Victor TC. Differential expression of genes in clinical strains of Mycobacterium Tuberculosis in response to isoniazid. Poster presented at the MRC Research Day, MRC Conference Centre, Cape Town, South Africa, 14-15 October 2010.
  • De Souza GA, Fortuin S, Aguilar D, Hernandez-Pando R, McEvoy CRE, Van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG. A label-free method identified differentially abundant proteins in related M. tuberculosis Beijing strains. Poster presented at the ESF 4th conference on Functional Genomics and Disease, Dresden, Germany, 14 – 17 April 2010.
  • Möller M, Flachsbart F, Till A, Thye T, Horstmann R, Meyer C, Osei I, Van Helden PD, Hoal EG, Schreiber S, Nebel A, Franke A. A functional haplotype in the 3’UTR of TNFRSF1B is associated with TB in two African populations. Poster presented at the EMBO|EMBL Symposium Human Variation: Cause and Consequence, Heidelberg, Germany, 20-23 June 2010.
  • Hoal EG, Cobat A, Gallant C, Simkin L, Black GF, Stanley K, Hughes J, Doherty T, Hanekom W, Eley B, Beyers N, Jaïs J, Boland-Auge A, Van Helden PD, Casanova J, Abel L, Alcaïs A, Schurr E. A Genome-Wide Linkage Search for Genetic Loci Controlling Resistance to Tuberculosis (1001/W). Poster presented at the 60th Annual Meeting of The American Society of Human Genetics, Washington DC, USA, 2-6 November 2010.
  • Möller M, De Wit E, Delport W, Harmant C, Rugamika CE, Quach H, Meintjes A, Balanovsky O, Zaporozhchenko V, Bormans C, Van Helden PD, Seioghe C, Behar DM, Hoal EG. The History in our genes: The complex structure of the South African Coloured population. Poster presented at the ASHG 60th Annual Meeting in Washington DC, USA, 2-6 November 2010.
  • Salie M, Möller M, Van Helden PD, Schreiber S, Hoal EG, Nebel A, Hofmann S. A 3’UTR polymorphism in ANXA11 is associated with pulmonary tuberculosis in the South African coloured population. Poster presented at the ASHG 60th Annual Meeting in Washington DC, USA, 2-6 November 2010. 

Other Relevant Outputs (including honours and awards to staff)

  1. Prof. Mizrahi delivered the Division U Mycobacteriology Lecture at the 110th Meeting of the American Society for Microbiology in San Diego, on 24th May 2010. This honour is given to a scientist who has made a significant contribution to the field of mycobacteriology, and is accompanied by the award of a certificate to mark the occasion.
  2. Prof. Mizrahi was appointed to three-year terms on the Board of Directors and the Scientific Advisory Board of K-RITH, UKZN.
  3. Dr. Bavesh Kana was appointed as the new Head of the Wits University node of the CBTBR, effective 1 January 2011.
  4. Eileen Hoal-van Helden received the SU Rector’s award for Excellence in Research in November 2010
  5. Warren, RM. Obtained a B2 rating by the NRF (2010)
  6. Gey van Pittius, NC was elected as a member of the Academy of Science of South Africa (ASSAf)
  7. Specialist diagnostic service for suspect extra-pulmonary TB cases
  8. Hospital medical specialist clinical services, eg pulmonology and genetics
  9. Advice to National Dept Agriculture on GMOs and veterinary TB
  10. Seegene (Korea): We have been approached and are currently in a collaborative venture with Seegene, Inc. to design and improve M. tuberculosis diagnostics.  Seegene is a molecular diagnostic company based in Korea well known for its pioneering R & D activities and novel technologies. Seegene, Inc. has continued to develop innovative technology platforms, ACP™ (Annealing Control Primer), DPO™ (Dual Priming Oligonucleotide), and READ™ (Real Amplicon Detection). With these cutting-edge molecular diagnostic technologies applied to diagnostic kits and various practical approaches, Seegene has enhanced the sensitivity and specificity of PCR (polymerase chain reaction) up to unprecedented level. This advance enables Seegene Inc. to provide multiplex PCR products by which multiple target genes of pathogens can be simultaneously detected, saving time and cost.
  11. AID (Germany): Evaluation of a genetic drug susceptibility test.
  12. Hain Life Sciences (Germany): Evaluation of the MDRTBsl genetic drug susceptibility test.
2009

Books / Chapters in Books (Total: 2)

  • Kana BD, Machowski EE, Rubin H, Mizrahi V. (2009) Electron transport and respiration. In: Mycobacterium: Genomics and Molecular Biology (Parish T & Brown A, eds.) Horizon Press, ch. 3, pp. 35-64.
  • McEvoy CRE, Warren RM, van Helden PD. 2009. Molecular Methods and Their Application in TB Epidemiology. In: Schaaf HS, Zumla AI (ed.) Tuberculosis. A Comprehensive Clinical Reference. Elsevier Publications. pp 28 – 37.

Articles in Peer-Reviewed Journals (Total: 41)

  • Mowa MB, Warner DF, Kaplan G, Kana BD, Mizrahi V. (2009) Function and regulation of class I ribonucleotide reductase-encoding genes in mycobacteria. J Bacteriol. 191(3):985-995. (IF=3.636)
  • Kana BD, Bonazzi M, Calzavara-Silva CE. (2009) The molecular and cellular basis of infection - perspectives from the first advanced summer school in Africa. IUBMB Life 61:85-90. (IF=2.364)
  • Abdallah AM, Verboom T, Weerdenburg E, Gey van Pittius NC, Mahasha PW, Jiménez C, Parra M, Cadieux N, Brennan MJ, Appelmelk BJ, Bitter W. (2009) PPE and PE_PGRS proteins of Mycobacterium marinum are transported via the type VII secretion system ESX-5, Mol. Microbiol.73(3):329–340. (IF=5.213)
  • Bezuidenhout J, Roberts T, Muller L, van Helden P, Walzl G.(2009) Pleural tuberculosis in patients with early HIV infection is associated with increased TNF-alpha expression and necrosis in granulomas. PLoS ONE. 4(1):e4228. (IF unavailable)
  • Bitter W, Houben ENG, Bottai D, Brodin P, Brown EJ, Cox JS, Derbyshire K, Fortune SM, Gao L-Y, Liu J, Gey van Pittius NC, Pym AS, Rubin EJ, Sherman DR, Cole ST, Brosch R. (2009) Systematic genetic nomenclature for type VII secretion systems, PLoS Pathogens Oct;5(10):e1000507 (IF=9.125)
  • Black GF, Thiel BA, Ota M, Parida SK, Adegbola R, Boom WH, Dockrell HM, Franken KLMC, Friggen AH, Hill PC, Klein MR, Lalor MK, Mayanja H, Schoolnik G, Stanley K, Weldingh K, Kaufmann SHE, Walzl G, Ottenhoff THM, The GCGH Biomarkers for TB Consortium. (2009) Immunogenicity of novel DosR regulon-encoded candidate antigens of Mycobacterium tuberculosis in three high-burden populations in Africa. Clin Vaccine Immunol. 16(8):1203-1212. (IF=2.237)
  • Chaoui I, Sabouni R, Kourout M, Jordaan AM, Lahlou O, Elouad R, Akrim M, Victor TC, Elmzibri M. (2009) Analysis of Isoniazid, Streptomycin and Ethambutol resistance in Mycobacterium tuberculosis isolates from Morocco. J. Infect. Dev. Ctries. 2009; 3(4):278-284. (IF unavailable)
  • Chegou NN, Black GF, Kidd M, van Helden PD, Walzl G. (2009) Host markers in Quantiferon supernatants differentiate active TB from latent TB infection: preliminary report. BMC Pulmonary Medicine. 9:21. (IF unavailable)
  • Cobat A, Gallant CJ, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Jaïs JP, Boland-Auge A, van Helden P, Casanova JL, Abel L, Hoal EG, Schurr E, Alcaïs A. (2009) Two loci control tuberculin skin test reactivity in an area hyperendemic for tuberculosis. J Exp Med. 206(12):2583-2591. (IF=15.219)
  • Detjen AK, Loebenberg L, Grewal HM, Stanley K, Gutschmidt A, Kruger C, Du PN, Kidd M, Beyers N, Walzl G, Hesseling AC. (2009) Short-term Reproducibility of a Commercial Interferon-gamma Release Assay. Clin. Vaccine Immunol. 16(8):1170-1175. (IF=2.237)
  • Diacon AH, Pym A, Grobusch M, Patientia R, Rustomjee R, Page-Shipp L, Pistorius C, Krause R, Bogoshi M, Churchyard G, Venter A, Allen J, Palomino JC, De Marez T, van Heeswijk RPG, Lounis N, Meyvisch P, Verbeeck J, Parys W, de Beule K, Andries K, Mc Neeley DF. (2009) The Diarylquinoline TMC207 for Multidrug-Resistant Tuberculosis. New Engl. J. Med. 360(23):2397-2405. (IF=50.017)
  • Djoba Siawaya JF, Beyers, N, van Helden PD, Walzl G. (2009) Differential Cytokine Secretion and Early Treatment Response in Patients with Pulmonary Tuberculosis. Clin. Exp. Immunol. 156(1):69-77. (IF=2.853)
  • Djoba Siawaya JF, Chegou N, Van Den Heuvel MM, Diacon AH, Beyers N, Van Helden PD, Walzl G.(2009) Differential cytokinee/chemokines and kl-6 profiles in patients with different forms of tuberculosis. Cytokine 47(2):132-136. (IF=2.214)
  • Donald PR, van Helden PD. (2009) The Global Burden of Tuberculosis - Combating Drug Resistance in Difficult Times. New Engl. J. Med. 360(23):2393-2395. (IF=50.017)
  • Espie IW, Hlokwe TM, Gey van Pittius NC, Lane E, Tordiffe ASW, Michel AL, Müller A, Kotze A, and van Helden PD. (2009) A case of pulmonary infection due to Mycobacterium bovis in a Black Rhinoceros (Diceros bicornis minor) in South Africa. J. Wildlife Dis. 45(4):1187-1193 (IF=1.330)
  • Hayward D, van Helden PD, Wiid I. (2009) Glutamine synthetase sequence evolution in the mycobacteria and their use as molecular markers for Actinobacteria speciation. BMC Evolution. Biol. 9:48. (IF=4.050)
  • Hesseling AC, Mandalakas AM, Kirchner LH, Chegou NN, Marais BJ, Zhu X, Black GF, Stanley K, Beyers N, Walzl G. (2009) Highly Discordant T-Cell Responses In Individuals With Recent Household Tuberculosis Exposure. Thorax 64(10):840-846. (IF=7.069)
  • Hoek KG, Schaaf HS, Gey van Pittius NC, van Helden PD, Warren RM. (2009) Resistance to pyrazinamide and ethambutol compromises MDR/XDR-TB treatment. S. Afr. Med. J. 99(11), 785-787. (IF unavailable)
  • Kagina BM, Abel B, Bowmaker M, Scriba TJ, Gelderbloem S, Smit E, Erasmus M, Nene N, Walzl G, Black G, Hussey GD, Hesseling AC, Hanekom WA. (2009) Delaying BCG vaccination from birth to 10 weeks of age may result in an enhanced memory CD4 T cell response. Vaccine.  27(40): 5488-5495. (IF=3.298)
  • Louw GE, Warren RM, Gey van Pittius NC, McEvoy CR, Van Helden PD, Victor TC. (2009) A balancing act: Efflux/influx in mycobacterial drug resistance, Antimicrob. Agents Ch. 53(8):3181-3189. (IF=4.716)
  • Louw GE, Warren RM, van Helden PD, Victor TC. (2009) Rv2629 191A/C nucleotide change is not associated with RIF resistance in Mycobacterium tuberculosis. Clin. Chem. Lab. Med. 47(4):500-501. (IF=1.888)
  • Marais BJ, Hesseling AC, Schaaf HS, Gie RP, van Helden PD, Warren RM. (2009) Mycobacterium tuberculosis transmission is not related to household genotype in a highly endemic setting. J. Clin. Microbiol. 74(5):1338-1343. (IF=3.945)
  • Marcotty T, Matthys F, Godfroid J, Rigouts L, Ameni G, Gey van Pittius NC, Kazwala R, Muma J, Van Helden PD, Walravens K, De Klerk LM, Geoghegan C, Mbotha D, Otte M, Amenu K, Abu Samra N, Botha C, Ekron M, Jenkins A, Jori F, Kriek N, McCrindle C, Michel A, Morar D, Roger F, Thys E, and Van den Bossche P. (2009) Zoonotic tuberculosis and brucellosis in Africa: neglected zoonoses or minor public health issues? Ann. Trop. Med. Parasit. 103(5):401-411. (IF=1.652)
  • McEvoy CRE, van Helden PD, Warren RM, Gey van Pittius NC. Evidence for a rapid rate of molecular evolution at the hypervariable and immunogenic Mycobacterium tuberculosis PPE38 gene region. BMC Evolution. Biol. 9:237. (IF=4.050)
  • McEvoy CRE, Warren RM, van Helden PD, Gey van Pittius NC. (2009) Multiple, independent, identical IS6110 insertions in Mycobacterium tuberculosis PPE genes. Tuberculosis 89:439-442. (IF=1.758)
  • McIlleron H, Willemse M, Werely CJ, Hussey GD, Schaaf HS, Smith PJ, Donald PR. (2009) Isoniazid Plasma Concentrations in a Cohort of South African Children with Tuberculosis: Implications for International Pediatric Dosing Guidelines. Clin. Infect. Dis. 48(11):1547-1553. (IF=8.266)
  • Michel AL, Coetzee ML, Keet DF, Mare L, Warren RM, Cooper D, Bengis RG, Kremer K, van Helden PD. (2009) Molecular epidemiology of Mycobacterium bovis isolates from free ranging wildlife in South African game reserves. Vet. Microbiol. 133: 335–343. (IF=2.370)
  • Möller M, Nebel A, Valentonyte R, van Helden P, Schreiber S, Hoal E. (2009)  Investigation of chromosome 17 candidate genes in susceptibility to TB in a South African population. Tuberculosis 89:189-194. (IF=1.758)
  • Müller B, Hilty M, Berg S, Garcia-Pelayo MC, Dale J, Boschiroli ML, Cadmus S, Naré B, Ngandolo R, Godreuil S, Diguimbaye-Djaibé C, Kazwala R, Bonfoh B, Njanpop-Lafourcade BM, Sahraoui N, Guetarni D, Aseffa A, Mekonnen MH, Razanamparany VR, Ramarokoto H, Djønne B, Oloya J, Machado A, Mucavele C, Skjerve E, Portaels F, Rigouts L, Michel A, Müller A, Källenius G, van Helden PD, Hewinson RG, Zinsstag J, Gordon SV, SmithNH. (2009) African 1; An Epidemiologically Important Clonal Complex of Mycobacterium bovis Dominant in Mali, Nigeria, Cameroon and Chad. J Bacteriol. 191(6):1951-1960. (IF=3.636)
  • Nusbaum C, Ohsumi TK, Gomez J, Aquadro J, Victor TC, Warren RM, Hung DT, Birren BW, Lander ES, Jaffe DB. (2009) Sensitive and specific polymorphism discovery in bacteria using massively parallel sequencing. Nature Methods. 6(1):67-69. (IF=13.651)
  • Onajole OK, Govender K, Govender P, van Helden PD, Kruger HG, Maguire GEM, Muthusamy K, Pillay M, Wiid I, Govender T. (2009) Pentacyclo-undecane derived cyclic tetra-amines: Synthesis and Evaluation as potent anti-tuberculosis agents. Eur. J. Med. Chem. 4297–4305. (IF=2.882)
  • Parsons SD, Gous TA, de Villiers C, Warren RM, Seier JV, van Helden PD. (2009) Detection of Mycobacterium tuberculosis infection in chacma baboons (Papio ursinus) using the QuantiFERON-TB Gold (In-Tube Method) assay. J. Med Primatol 38:411-417.  (IF=1.047)
  • Schaaf HS, Victor TC, Venter A, Brittle W, Hesseling AC, Marais BJ, van Helden PD, Donald PR. (2009) Ethionamide cross- and co-resistance in children with isoniazid-resistant tuberculosis. Int. J. Tuberc. Lung. D. 13(11):1355-1359. (IF=2.304)
  • Uys PW, van Helden PD, Hargrove JW. (2009) Tuberculosis reinfection rate as a proportion of total infection rate correlates with the logarithm of the incidence rate – a mathematical model.  J Roy Soc Interface. 6(30):11-15. (IF=3.621)
  • Uys PW, Warren R, van Helden PD, Murray M, Victor TC. (2009) The Potential of Rapid Diagnosis for Controlling Drug-Susceptible and Drug-Resistant TB in High Prevalence Communities. J Clin Microbiol. 47(5):1484-1490. (IF=3.945)
  • Van der Spuy GD, Kremer K, Ndabambi SL, Beyers N, Dunbar R, Marais BJ, van Helden PD, Warren RM.  (2009) Changing Mycobacterium tuberculosis population highlights clade-specific pathogenic characteristics. Tuberculosis. 89(2)120-125. (IF=1.758)
  • Van der Spuy GD, van Helden PD, Warren RM. (2009). Effect of study duration on the interpretation of tuberculosis molecular epidemiology investigations. Tuberculosis. 89:238-242. (IF=1.758)
  • Van der Spuy GD, Warren RM, van Helden PD. (2009) The role of molecular epidemiology in low-income, high-burden countries. Int.J.Tuberc.Lung Dis. 13:419-420. (IF=2.304)
  • Warren RM, Streicher EM, Gey van Pittius NC, Marais BJ, van der Spuy GD, Victor TC, Sirgel F, Donald PR, van Helden PD. (2009) The clinical relevance of Mycobacterial pharmacogenetics. Tuberculosis. 89(3):199-202. (IF=1.758)
  • Wright CA, Hesseling AC, Bamford C, Bergess SM, Warren RM, Marais BJ. (2009) Fine Needle Aspiration Biopsy-A first line diagnostic procedure in paediatric tuberculosis? Int. J. Tuberc. Lung. D. 13:1373-1379. (IF=2.304)
  • Wright CA, Warren RM, Marais BJ. (2009) Fine Needle Aspiration Biopsy-A (FNAB) – an undervalued diagnostic modality in paediatric mycobacterial disease. Int J Tuberc Lung D. 13:1467-1475. (IF=2.304)

Non Peer-Reviewed Articles (Total: 2)

  • Marais BJ, Parker SK, Verver S, Van Rie A, Warren RM. (2009) Primary and Postprimary or Reactivation Tuberculosis: Time to Revise Confusing Terminology? Am. J. Roentgenol. (Letter) 192:W10361-803X/019X1924-W1. (IF=2.940)
  • van der Spuy GD, Warren RM, van Helden PD. (2009) "The role of molecular epidemiology in low-income (high-burden) countries" (Editorial), Int. J. Tuberc Lung D.  13(4):419-420. (IF=2.304)

Published Abstracts (Total: 0)

Technical Reports (Total: 1)

  • IAEA (Int Atomic Energy Agency): SAF6008 Progress report on drug resistance project for SA (June 2009).

Products / Artefacts / Patents (Total: 0)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 103)

  • Warner DF, Mowa MB, Machowski EE, Kaplan G, Kana BD, Mizrahi V. Novel and vulnerable pathways in DNA metabolism. Plenary lecture, Keystone Symposium on Tuberculosis: Biology, Pathology & Therapy, Keystone, CO, 25-30 January 2009.
  • Kana BD.  Resuscitation promoting factors in M. tuberculosis. Human Genetics Seminar Series, NHLS Braamfontein, 19 February 2009.
  • Warner DF. Physiology of M. tuberculosis – Chinks in the armour. Invited lecture, SATBAT conference on the Molecular Epidemiology of TB, Sunnyside Park Hotel, Johannesburg, 26-27 March 2009.
  • Mizrahi V. Survival, subversion and stress response strategies in Mycobacterium tuberculosis: Implication for Drug Discovery. Plenary lecture presented at the ESF Europe-Africa Frontier Research Conference on Infectious Diseases: From Basic to Translational Research, Cape Winelands, 4-9 April 2009.
  • Mizrahi V. Survival, subversion and stress response strategies of M. tuberculosis: Implications for TB drug discovery. Plenary lecture presented at the 109th General Meeting of the American Society for Microbiology, Philadelphia, 18-22 May 2009.
  • Kana BD. Resuscitation promoting factors in Mycobacterium tuberculosis. Lecture presented at the MRC TB Colloquium, Cape Town 3-4 June 2009.
  • Warner DF. Mycobacterium and mechanisms of mutagenesis. Lecture presented at the MRC TB Colloquium, Cape Town 3-4 June 2009.
  • Mizrahi V. Panel Member: TB, Where We Stand and Where We Can Go. Global Health Research Congress, Seattle, USA, 15-18 June 2009.
  • Kana BD, Gordhan BG, Downing KJ, Sung N, Vostroktunova G, Machowski EE, Tsenova L, Young M, Kaplreylants A, Kaplan G, Mizrahi V. Resuscitation promoting factors are required for the virulence of Mycobacterium tuberculosis but are collectively dispensable for growth in vitro. Invited talk presented at the Third Congress of European Microbiologists, hosted by the Federation of European Microbiological Societies (FEMS), Gothenberg, Sweden, 28 June – 2 July 2009.
  • Kana BD, Abrahams GL, Sung N, Warner DF, Gordhan BG, Machowski EE, Tsenova L, Sacchettini J, Stoker NG, Kaplan G, Mizrahi V. Role of the Y-family polymerase-encoding dinB homologues in Mycobacterium tuberculosis. Poster presented at the Third Congress of European Microbiologists, hosted by the Federation of European Microbiological Societies (FEMS), Gothenberg, Sweden, 28 June – 2 July 2009.
  • Moosa A, Mizrahi V, Warner DF. The metabolism of vitamin B12 in Mycobacterium tuberculosis: the role of pseudovitamin B12. Talk presented at the SATBAT Meeting, Lung Institute, UCT, 18 July 2009.
  • Williams M, Kana BD, Mizrahi V. Analysis of molybdopterin cofactor biosynthetic genes in mycobacteria. Invited talk presented at the 6th Gordon Research Conference on Molybdenum & Tungsten Enzymes, Lucca, Italy, 5-10 July 2009.
  • Gordhan BG. Improving the budget approval process for improved purchasing power and maintenance management. Invited talk presented a talk at the Intelligent Laboratory Management Conference, Rosebank, Johannesburg, 27 July 2009.
  • Warner DF. Transport and function of vitamin B12 in mycobacteria. Invited talk presented at the EPFL, Lausanne, Switzerland, 31 July 2009.
  • Moosa A, Mizrahi V, Warner DF. The metabolism of vitamin B12 in Mycobacterium tuberculosis: role of alternate B12 cofactors. Invited short talk presented at the Gordon Research Conference on Vitamin B12 and Corphins, 2-7 August 2009.
  • Mizrahi V. Target identification and validation. Invited talk presented at the CSIR/ICS-UNIDO Workshop on the Advanced Design and Development of Potential Drugs Against Tuberculosis. Pretoria, 3-5 August 2009.
  • Mizrahi V. Novel Tuberculosis Drug Target Identification and Validation. Haemolotogy & Molecular Medicine Seminar, University of the Witwatersrand, Johannesburg, 12 August 2009.
  • Abrahams GL, Mizrahi V. Target-based whole-cell screening. Talk presented at the Annual Meeting of the IMTB Consortium, Magdalen College, Oxford, 15-16 August 2009.
  • Kana BD.  Resuscitation-promoting factors in M. tuberculosis: Invited talk presented at the Imperial College of London, 24 August 2009.
  • Kana BD. Resuscitation-promoting factors in Mycobacterium tuberculosis - Wake Up TB. Invited talk presented at the MRC Career Awards Symposium, Medical Research Council, Cape Town, 19 October 2009.
  • Moosa A, Mizrahi V, Warner DF. Mechanisms of vitamin B12 transport and regulation in mycobacteria. Invited talk presented at the 2nd Postgraduate Cross-Faculty Symposium, University of the Witwatersrand, 20-21 October 2009. [Awarded Second prize in Science Faculty Oral Presentation category]
  • Abrahams GL, Mowa MB, Magwira C, Savvi S, Warner DF, Kana BD, Mizrahi V. Novel TB drug target identification and validation. Talk presented at Human Genetics Seminar Series, University of the Witwatersrand & NHLS, Johannesburg, 26 November 2009.
  • Mizrahi V. The DST/NRF Centre of Excellence for Biomedical TB Research. The Centres of Excellence Annual Function, STIAS, Stellenbosch, 26 November 2009.
  • Abrahams GL, Mowa MB, Magwira C, Savvi S, Warner DF, Kana BD, Mizrahi V. Novel TB drug target identification and validation. Invited talk presented at AstraZeneca India. Bangalore, India, 9 December 2009.
  • Warren RM. Laboratory Based Research at the Faculty of Health Sciences. Invited talk presented to the Harvard Delegation. Stellenbosch University. Stellenbosch. South Africa, January 2009
  • Walzl G. The possible implementation of Quantiferon TB by the Department of Health. Invited Talk at the National Department of Health / Pro-Gen Diagnostics Workshop. Johannesburg, South Africa, 02 March 2009.
  • McEvoy CRE, Cloete REA, Gey van Pittius NC. Mycobacterium tuberculosis PE and PPE gene family genetic polymorphisms: antigenic variation or evolution? Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Newton-Foot M, Steyn A, Sampson S, Warren RM, van Helden PD, Gey van Pittius NC. The M. tuberculosis ESX-3 secretion system interactome Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Warren RM. XDR-TB: a molecular epidemiological perspective. Invited talk at the World TB Day. 2 Military Hospital ,Cape Town, South Africa, 02 March 2009.
  • Warren RM. South African Molecular Epidemiological Data. Invited talk presented at the SATBAT Workshop - Promoting Innovative TB Research Through Training. Johannesburg, South Africa, 26 March 2009.
  • Hoal EG. Host genetic susceptibility to tuberculosis. Talk presented at European Science Foundation: Europe-Africa Frontier Research Conference, Infectious Diseases: From Basic to Translational Research. Somerset West, South Africa,  4-9 April 2009.
  • Hoal EG. Whole Genome Scans in Tuberculosis. Talk presented at 13th South African Society for Human Genetics Congress. Stellenbosch, South Africa, 7 April 2009.
  • van Helden PD. Science versus dogmas in combating the TB scourge. Invited talk presented at the Royal Society of South Africa meeting held in Cape Town, South Africa, 15 April 2009.
  • McEvoy CRE. Science in the media: opinions from a practicing scientist. Invited talk at the Stellenbosch university Honours journalism class. Stellenbosch, South Africa, 22 May 2009.
  • Gey van Pittius NC. Evolution, duplication and variation of the PE and PPE multigene families of Mycobacterium tuberculosis . Invited talk presented at the 109th General Meeting of the American Society for Microbiology (ASM)  Philadelphia, Pennsylvania, USA, 17-21 May 2009.
  • van Helden PD. Keynote Address presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Victor TC. Mechanisms of drug-resistance. Talk presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Warren RM. Genetic Epidemiology of TB in the Western Cape. Talk presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Chegou NN. Differentiate active TB from Latent TB infection: preliminary report. Talk presented at the 2nd Global Symposium on Interferon Gamma Release Assays. Dubrovnik, Croatia, 30 May-01 June 2009.
  • van Helden PD. Molecular epidemiology: role for transmission of MDR/XDR.  Talk presented at the WHO Catalysing HIV/TB research: innovation, funding and networking meeting. Cape Town, South Africa, 18-19 July 2009.
  • Hoek KGP, Gey van Pittius NC, van Helden PD, Warren RM. Single closed tube detection of Drug-resistance in mycobacterium tuberculosis complex. Talk presented at the NIH ICORTA South Africa TB Aids Training Program meeting. Cape Town, South Africa, 18 July 2009.
  • Harper CJ, Hayward D, Wiid I, Van Helden PD. Elucidation Of The Physiological Roles Of Glutamine Synthetase And Glutamate Dehydrogenase In Mycobacterium Smegmatis. Talk presented at the 52nd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Salie M, Hoal EG, Möller M. An Investigation Of Purinergic P2x7 Receptor And Toll-Like Receptor 1 In Susceptibility To Human Pulmonary Tuberculosis. Talk presented at the 52nd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • McEvoy CRE. The Mycobacterium tuberculosis Genome: Insights into Evolution, Epidemiology and Phylogeography. Invited talk at the University of Cape Town Department of Molecular and Cellular Biology Seminar Series. Cape Town, South Africa, 14 August 2009.
  • Gey van Pittius N.C. Tuberculosis Research at the Faculty of Health Sciences of the University of Stellenbosch. Invited talk presented at the Centre for Aids Research (CFAR) Colorado Tuberculosis Workshop. Fort Collins, Colorado, USA, 09 September 2009.
  • McEvoy CRE. Extensive analysis of PE and PPE gene variation in Mycobacterium tuberculosis. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Warren RM. A label-free method identified differentially abundant proteins in related Mycobacterium tuberculosis Beijing genotype. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Warren RM. Adaptaion of Mycobacterium tuberculosis gene expression in response to the evolution of rifampicin-resistance. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Hanekom M. Increased bacterial load is associated with strains of the Mycobacterium tuberculosis Beijing genotype. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Hanekom M. Mixed Mycobacterium tuberculosis infection in patients resident in a high HIV prevalence setting. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Gey van Pittius NC. The evolution of genus Mycobacterium and variation in the M. tuberculosis complex. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Victor TC. Beijing genotype Mycobacterium tuberculosis strains drive the drug-resistant tuberculosis epidemic in the Western and Eastern Cape regions of Soth Africa. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Streicher EM. XDR-TB: Acquisition of ofloxacin Resistance during treatment. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Hoek KGP. Genotypic based diagnosis of drug-resistance in Mycobacterium tuberculosis complex and treatment implications. Invited talk at the TBadapt 3rd meeting on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resisitance. Cape Town, South Africa, 16-18 September 2009.
  • Van Helden PD. TB Transmission between Wild and domestic Animals in South Africa. Invited talk at the 49th Interscience Conference on Antimicrobial Agents and Chemotherapy held in San Francisco, California, USA,12-15 September 2009.
  • Van Helden PD. TB Transmission between Wild and domestic Animals in South Africa. Invited talk at the University of California, Berkeley, USA 17 September 2009.
  • Walzl G. Bio-signatures for TB: how close are we? Thaler lecture, Mini-Symposium on TB Biomarkers and Development of New Vaccines hosted by the Aeras Global TB Vaccine Initiative. Washington DC, USA, 21 September, 2009.
  • Van Helden PD. TB Genomics and TB/HIV in Africa. Invited talk presented at the Keystone Symposia on Overcoming the Crisis of TB and AIDS. Arusha, Tanzania, 20-25 October 2009.
  • Warren RM. Adaptation of Mycobacterium tuberculosis gene expression in response to the evolution of rifampicin-resistance. Invited talk presented at the TB Adapt Meeting, Stellenbosch. November 2009.
  • Warren RM. TB Adapt South Africa. Invited talk presented at the TB Adapt Meeting, Stellenbosch. November 2009.
  • van Helden PD. The DST/NRF Centre of Excellence for Biomedical TB Research. The Centres of Excellence Annual Function, STIAS, Stellenbosch, 26 November 2009.
  • Walzl G. How do complex cytokine patterns improve our understanding of protection against TB? South African Immunology Society Conference, Cape Town, South Africa, 9-11 December 2009.
  • Warren RM.  Molecular Epidemiology of drug resistant tuberculosis. Invited talk presented at the DAAD Summer School, Stellenbosch, South Africa, December 2009.
  • Gey van Pittius NC. The evolution of the genus Mycobacterium and virulence in the M. tuberculosis complex. Invited talk presented at the DAAD Summer School, Stellenbosch, South Africa, December 2009.
  • Moosa A, Mizrahi V, Warner DF. The metabolism of vitamin B12 in M. tuberculosis: the role of alternate B12 cofactors. Poster presented at the MRC TB Colloquium, Cape Town 3-4 June 2009.
  • Naran K, Mizrahi V, Warner DF. Characterisation of the antimycobacterial effect of a Pseudomonas-derived activity. Poster presented at the MRC TB Colloquium, Cape Town 3-4 June 2009.
  • Ndwandwe DE, Warner DF, Mizrahi V. Mechanisms of mutagenesis in M. tuberculosis: Structural and functional characterization of the polymerase accessory factors encoded by Rv3394c and Rv3395c. Poster presented at the MRC TB Colloquium, Cape Town 3-4 June 2009.
  • Naran K, Veale R, Mizrahi V, Warner DF. Characterization of the antimycobacterial effect of a Pseudomonas-derived activity. Poster presented at the 2nd Postgraduate Cross-Faculty Symposium, University of the Witwatersrand, 20-21 October 2009.
  • Ndwandwe DE, Venclovas Č, Mizrahi V, Warner DF. Mechanisms of mutagenesis in Mycobacterium tuberculosis: structural and functional characterization of the DNA polymerase accessory factors encoded by Rv3394c and Rv3395c. Poster presented at the 2nd Postgraduate Cross-Faculty Symposium, University of the Witwatersrand, 20-21 October 2009.
  • Bharuthram A, Mizrahi V, Gordhan BG. Construction and phenotypic characterization of M. smegmatis mutants deficient in DNA glycosylases. Poster presented at the 2nd Postgraduate Cross-Faculty Symposium, University of the Witwatersrand, 20-21 October 2009.
  • Mapela L, Kana BD. Characterization of resuscitation promoting factors in Mycobacterium smegmatis. Poster presented at the 2nd Postgraduate Cross-Faculty Symposium, University of the Witwatersrand, 20-21 October 2009.
  • Black GF, Loxton A, Ottenhoff TH, Haks M, Franken KLMC, Stanley K, Chegou N, Parida SK, Kaufmann SHE, Walzl G and the GC6-74 Consortium. Immunogenicity of novel M. tuberculosis antigens, cytokine profiles and gene expression patterns hold promise as markers of disease activity. Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Cliff J, Cho J-E, Whittaker J, van Helden P, Beyers N, Walzl G, Dockrell H. Identification of biomarkers to predict tuberculosis treatment outcome by transcriptomic analysis of peripheral blood. Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Govender L, Abel B. Hughes J, de Kock M, Choice T, Walzl G, Black G, Andersen P, Mohamed H, Hussey G, Hanekom W. Persons latently infected with M. tuberculosis have T cell responses to the novel antigens Rv2669 and Rv2660, whereas patients with active disease do not. Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Nene N, Walzl G, Ottenhoff T, Franken K, Stanley K, Parida SK, Kaufmann S, Black G. Immunity to tuberculous and non-tuberculous mycobacteria in adult TB contacts in the Western Cape. Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • McEvoy CRE, Cloete REA, Gey van Pittius NC, Mycobacterium tuberculosis PE and PPE gene family genetic polymorphisms: antigenic variation or evolution? Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Newton-Foot M, Steyn A, Sampson S, Warren RM., van Helden PD, Gey van Pittius NC, The M. tuberculosis ESX-3 secretion system interactome Poster presented at the Keystone Symposium on Tuberculosis: Biology, Pathology and Therapy, Keystone, USA, January 2009.
  • Hemmings SMJ, Tait M, Martin L, Aitken L, De Wit E, Black G, Hoal EG, Walzl G, Seedat S. Genetic markers associated with posttraumatic stress disorder in tuberculosis household contacts: a preliminary study. Biological Psychiatry Congress 2009, Arabella, Kleinmond.
  • Parsons SDC. Early detection of tuberculosis in chacma baboons (Papio ursinus) using the QuantiFERON-TB Gold assay. Poster presented at the International Meeting on Emerging Diseases. Vienna, Austria, 13-16 February 2009.
  • Chegou NN. Host markers in Quantiferon Supernatants differentiate active TB from Latent TB infection: preliminary report. Poster presented at the ESF Europe-Africa Frontier Research Conference on Infectious Diseases: From Basic to Translational Research. Cape Town, South Africa, 4-9 April 2009.
  • Fortuin S. Characterisation of Phosphoproteins in Mycobacterium tuberculosis. Poster presented at the ESF Europe-Africa Frontier Research Conference on Infectious Diseases: From Basic to Translational Research. Cape Town, South Africa, 4-9 April 2009.
  • Bester M, Warren RM, Johnson R, van Helden PD, Ndimba BK, Victor TC. Mechanisms that determine the level of rifampicin resistance in Mycobacterium tuberculosis. Poster presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Hoek KGP, Gey van Pittius NC, van Helden PD, Warren RM. Single tube detection of ethambutol resistance in mycobacterium tuberculosis complex. Poster presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Louw GE, Warren RM, Gey van Pittius NC, van Helden PD, Victor TC. New hope for treatment of drug resistant tuberculosis. Poster presented at the MRC TB Colloquium. Cape Town, South Africa, 3-4 June 2009.
  • Bruiners N, Gey van Pittius NC, Warren RM. Investigating the human M. tuberculosis interactome to identify the host targets of ESAT-6 and CFP-10. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Fang Z, Van Helden PD, Gey van Pittius NC. Elucidation of the substrates of mycosin 3, an essential protease of Mycobacterium tuberculosis. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Hoek KGP, Gey van Pittius NC, Van Helden PD, Warren RM. Single tube detection of ethambutol resistance in Mycobacterium tuberculosis complex. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Kruger C, Warren R, Gey van Pittius NC. Determining the incidence of non-tuberculous mycobacteria in environmental settings. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Louw GE, Warren RM, Gey van Pittius NC, Van Helden PD, New hope for treatment of drug resistant tuberculosis. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Newton-Foot M, Smit M, Gey van Pittius NC. The construction of genetic knockouts of the antigen secretion systems ESX-3 and -4 in Mycobacterium smegmatis. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009. 
  • Streicher EM, Warren RM, De Kock M, Bosman M, Gey van Pittius NC, Van Helden PD, Victor TC. Extensively drug-resistant tuberculosis (XDR-TB) in the Western Cape region of South Africa: acquisition vs transmission. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Van der Merwe RG, Gey van Pittius NC, Van Helden PD. Isolation and characterization of South African mycobacteriophages. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Hoek KGP, Schaaf HS, Gey van Pittius NC, Van Helden PD, Warren RM. Incidence of resistance to pyrazinamide and ethambutol compromises treatment regimens for MDR/XDR-TB treatment. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Mcevoy CRE, Van Helden PD, Warren RM, Gey Van Pittius NC. Rapid rate of molecular evolution at the hypervariable and immunogenic Mycobacterium tuberculosis PPE38 gene region. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Mlamla ZC, Louw GE, Warren R, Gey Van Pittius NC, Van Helden P, Victor T, Hoornenborg E, Taljaard JJ, Hoffmann R, Morkar J, Jordaan HF. Improving methods for genotypic drug resistance testing in Mycobacterium tuberculosis. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Hanekom M, Van De Berg D, Streicher EM, Cox H, Mcdermid C, Victor TC, Van Helden PD, Warren RW. Mixed Mycobacterium Tuberculosis Infection In Patients Isolates Resident In A High Hiv Prevalence Setting. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Hayward D, Harper CJ, Wiid I, Van Helden PD. Differences In Glutamine/ Glutamate Metabolism May Influence Mycobacterial Survival. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Kleynhans L, Mcconnell A, Walzl G. The Impact Of The Steroid Hormones Medroxyprogesterone Acetate, Cortisol And Progesterone On Protective Immunity To Tuberculosis. Poster presented at the 53rd Academic Year Day, University of Stellenbosch, 12-13 August 2009.
  • Louw GE, Warren RM, Gey van Pittius NC, van Helden PD, Victor TC. New hope for treatment of drug resistant tuberculosis. Poster presented at the Keystone Symposia on Overcoming the Crisis of TB and AIDS. Arusha, Tanzania, 20-25 October 2009.
  • Streicher EM, Gey van Pittius NC, Bosman M, van Helden PD, Victor TC, Warren RM. Acquisition of ofloxacin Resistance during treatment of Multidrug-Resistant Tuberculosis. Poster presented at the Keystone Symposia on Overcoming the Crisis of TB and AIDS. Arusha, Tanzania, 20-25 October 2009.
  • Prach L, Kirby J, Schelle M, Warren RM, van Helden PD, Keasling J, Alber T. Structure and biosynthesis of novel diterpines in Mycobacterium tuberculosis. Poster presented at the Keystone Symposia on Overcoming the Crisis of TB and AIDS. Arusha, Tanzania, 20-25 October 2009.
  • Wangh LJ, Rice J, Rahman L, Kreisworth B, Kramer FR, Warren RM. Construction of a highly Multiplexed LATE-PCR Assay for M(X)Dr-TB. Poster presented at the Keystone Symposia on Overcoming the Crisis of TB and AIDS. Arusha, Tanzania, 20-25 October 2009.
  • Loxton A, Roberts T, Black G, Walzl G. Regulatory T-cells and high levels of FOXP3 mRNA leads to decreased immune responses in HIV-TB co-infection. Poster presented at the AIDS Vaccine 2009 Conference. Paris, France, 19-23 October 2009.

Conferences / Meetings Hosted (Total 2)

  • The Stellenbosch University node held an International TB conference focusing on the Effect of Genetic Variation in Mtb on Vaccine Escape and the Acquisition of Drug Resistance, at Lanzerac wine estate between the 16th and 18th of September 2009.
  • The Stellenbosch University node and the Desmond Tutu TB Centre held a workshop with colleagues from George Washington University, Debra Benator, Fred Gordin and Donna Conwell from 24 to 27 February 2009 to work on a joint strategy for a SU application to the CDC-sponsored TB Trials Consortium (TBTC). This collaborative effort brings together a strong group of adult and paediatric TB and HIV researchers at the faculty in a joint consortium called TB SUN. TB SUN will aim to join larger collaborative TBTC international activities to implement trials of new TB therapies where most needed, including in children.

Other Relevant Outputs (including honours and awards to staff)

  1. Mizrahi V. Elected into Fellowship of the American Academy of Microbiology in January 2009 and inaugurated into the Academy at a function that took place in Philadelphia during the General Meeting of the American Society for Microbiology. The Academy, the honorific leadership group within the American Society for Microbiology, recognizes excellence, originality, and creativity in the microbiological sciences, and election to fellowship is a mark of distinction.
  2. Mizrahi, V. Awarded an A2 rating by the NRF
  3. Mizrahi V. Appointed to a three-year term on the Scientific Advisory Committee of the Global Alliance for TB Drug Development, which is based in New York. She attended SAC meetings in New York in June and December.
  4. Kana B. Awarded a Career Development Award from the MRC. He is the second member of the Wits node to receive this honour
  5. Kana B. Stage 1 application for the Friedel Sellschop Award successful and invited to submit a proposal for an individual research grant to the URC. This is the premier award from Wits for young researchers.
  6. Kana, BD. Rated as C3 category researcher by the NRF
  7. Van Helden, PD. Academy of Science of South Africa’s (ASSAf) Science-for-Society Gold Medal
  8. Van Helden, PD. Featured in an article on Thompson Reuters’ Sciencewatch, citing him as the 4th highest ranked scientist in the World in the field of tuberculosis
  9. Gey van Pittius, NC. Innovation Fund - Patent Incentive Fund Award (2009)
  10. Gey van Pittius, NC. Health Sciences Faculty Award for Outstanding Research (June 2009)
  11. Gey van Pittius, NC. Rated as Y1 category researcher by the NRF
  12. Gordhan, BG. Rated as C2 category researcher by the NRF
  13. Warner, DF. Rates as Y1 category researcher by the NRF
  14. Walzl G. Received the Rector’s Award for Outstanding Research in 2009
  15. Warren, RM. Rated as B2 category researcher by the NRF
2007

Books / Chapters in Books (Total: 1)

  • Mizrahi V. 2007. Mobilising the best brains to deal with poverty and health in Africa: Challenges and opportunities in the world of science. In: Biotechnology and Health: South Africa’s aspirations in health-related biotechnology (J. Chataway & W. James, eds.), Van Schaik Publishers, Pretoria, South Africa, ch. 8, pp. 42-44.

Articles in Peer-Reviewed Journals (Total: 31)

  • Machowski EE, Barichievy S, Böttger EC, Durbach SI, Mizrahi V. (2007) In vitro analysis of rates and spectra of mutations in a polymorphic region of the Rv1046 PE_PGRS gene of Mycobacterium tuberculosis. J. Bacteriol. 189:2190-2195. (IF=3.993)
  • Warner DF, Savvi S, Mizrahi V, Dawes SS. (2007) A riboswitch regulates expression of the coenzyme B12-dependent methionine synthase in Mycobacterium tuberculosis: implications for differential methionine synthase function in strains H37Rv and CDC1551. J. Bacteriol. 189:3655-3659. (IF=3.993)
  • Kana BD, Gordhan BG, Downing KJ, Sung N, Vostroktunova G, Machowski EE, Tsenova L, Young, M, Kapreylants A, Kaplan G, Mizrahi V. (2007) The resuscitation-promoting factors of Mycobacterium tuberculosis are required for virulence and resuscitation from dormancy but are collectively dispensable for growth in vitro. Mol. Microbiol. Dec 21 [Epub ahead of print] doi:10.1111/j.1365-2958.2007.06078.x (IF=5.634)
  • Biketov S, Potapov V, Kombarova T, Downing KJ, Kana BD, Kaprelyants AS. (2007) The role of resuscitation-promoting factors in resuscitation and reactivation of Mycobacterium tuberculosis during intraperitoneal infection in mice. BMC Infect Dis. 7:146. (IF=1.898)
  • Warner DF, Mizrahi V. (2007) The survival kit of Mycobacterium tuberculosis. Nat. Med. 13:282-284. (IF=28.588)
  • Victor TC, Streicher EM, Kewley C, Jordaan AM, van der Spuy GD,  Bosman M, Louw H, Murray M, Young D, van Helden PD, Warren RM (2007) Spread of an emerging Mycobacterium tuberculosis drug-resistant strain in the Western Cape of South Africa. Int. J. Tub. Lung Dis. 11:195-201. (IF=2.035)
  • Maree F, Hesseling AC, Schaaf HS, Marais BJ, Beyers N, van Helden P, Warren RN, Schoeman JF. (2007) Absence of an association between Mycobacterium tuberculosis genotype and clinical features in children with tuberculous meningitis. Pediatr. Infect. Dis. 26:8-13. (IF=3.215)
  • Möller M, Nebel A, Kwiatkowski R, van Helden PD, Hoal EG, Schreiber S. (2007) Host susceptibility to tuberculosis: CARD15 polymorphisms in a South African population. Mol. Cell Probes. 21:148-151. (IF=2.016)
  • Mistry R, Cliff JM, Clayton CL, Beyers N, Mohammed YS, Wilson PA, Dockrell HM, Wallace DM, van Helden PD, Duncan K, Lukey PT. (2007) Gene-Expression Patterns in Whole Blood Identify Subjects at Risk for Recurrent Tuberculosis. J. Infect. Dis. 195:357-365. (IF=5.363)
  • Streicher EM, Victor TC, van der Spuy G, Sola C, Rastogi N, van Helden PD, Warren RM. (2007) Spoligotype Signatures in the Mycobacterium tuberculosis Complex.  J. Clin. Microbiol. 45:237-240. (IF=3.445)
  • Babb C, Hanekom-Keet EH, van Helden PD, Hoal EG (2007) SP110 polymorphisms are not associated with pulmonary tuberculosis in a South African population.  Hum. Genet. 8 121:521-522. (IF=3.662)
  • Schaaf HS, Victor TC, Engelke E, Brittle W, Marais BJ, Hesseling AC, van Helden PD, Donald PR. (2007) Minimal inhibitory concentration of isoniazid in isoniazid-resistant Mycobacterium tuberculosis isolates from children.  Eur. J. Clin. Microbiol. Infect. Dis. 26:203-205. (IF=2.33)
  • Babb C, van der Merwe L, Beyers N, Pheiffer C, Walzl G, Duncan K, van Helden P and Hoal EG.  (2007) Vitamin D receptor gene polymorphisms and sputum conversion time in pulmonary tuberculosis patients.  Tuberculosis (Edinb). 87:295-302. (IF=3.425)
  • Barreiro LB, Neyrolles O, Babb C, van Helden PD, Giquel B, Hoal E, Quintana-Murci L. (2007) Length variation of DC-SIGN and L-SIGN neck -region has no impact on tuberculosis susceptibility. Human Immunology 68:106-112. (IF=2.605)
  • Roberts T, Beyers N, Aguirre A, Walzl G. (2007)  Immunosuppression during active tuberculosis is characterized by decreased interferon – gamma Production and CD25 Expression with elevated forkhead Box P3, Transforming Growth Factor – and interleukin-mRNA levels.  J Infect Dis. 195:870-878. (IF=5.363)
  • Möller M, Kwiatkowski R, Nebel A, van Helden PD, Hoal EG, Schreiber S. (2007) Allelic variation in BTNL2 and susceptibility to tuberculosis in a South African population.  Microbes Infect. 9:522-528. (IF=3.127)
  • Diacon AH, Patientia RF, Venter A, van Helden PD, Smith PJ, McIlleron H, Maritz JS, Donald PR. (2007) Early bactericidal activity of high-dose rifampin in patients with pulmonary tuberculosis evidenced by positive sputum smears. Antimicrob. Agents Chemother. 51:2994-2996. (IF=4.153)
  • Donald PR, Parkin DP, Seifart HI, Schaaf HS, van Helden PD, Werely CJ, Sirgel FA, Venter A, Maritz JS. (2007) The influence of dose and N-acetyltransferase-2 (NAT2) genotype and phenotype on the pharmacokinetics and pharmacodynamics of isoniazid. Eur. J. Clin. Pharmacol. 63:633-639. (IF=2.029)
  • Hanekom M, van der Spuy GD, van Pittius NC, McEvoy CR, Ndabambi SL, Victor TC, Hoal EG, van Helden PD, Warren RM. (2007) Evidence that the Spread of Mycobacterium tuberculosis Strains with the Beijing Genotype Is Human Population Dependent. J. Clin. Microbiol. 45:2263-2266. (IF=3.445)
  • Hanekom M, van der Spuy GD, Streicher E, Ndabambi SL, McEvoy CR, Kidd M, Beyers N, Victor TC, van Helden PD, Warren RM. (2007) A recently evolved sublineage of the Mycobacterium tuberculosis Beijing strain family is associated with an increased ability to spread and cause disease. J. Clin. Microbiol. 45:1483-1490. (IF=3.445)
  • Chihota V, Apers L, Mungofa S, Kasongo W, Nyoni IM, Tembwe R, Mbulo G, Tembo M, Streicher EM, van der Spuy GD, Victor TC, van Helden P, Warren RM. (2007) Predominance of a single genotype of Mycobacterium tuberculosis in regions of Southern Africa. Int. J. Tuberc. Lung Dis. 11:311-318. (IF=2.035)
  • Veenstra H, Crous I, Brahmbhatt S, Lukey P, Beyers N, van Helden PD, Walzl G. (2007) Changes in the kinetics of intracellular IFN-gamma production in TB patients during treatment. Clin. Immunol. 124:336-344. (IF=3.606)
  • Djoba Siawaya JF, Ruhwald M, Eugen-Olsen J, Walzl G. (2007) Correlates for disease progression and prognosis during concurrent HIV/TB infection. Int. J. Infect. Dis. 11:289-299. (IF=2.062)
  • Werely CJ, Donald PR, van Helden PD. (2007) NAT2 polymorphisms and their influence on the pharmacology and toxicity of isoniazid in TB patients. Personalized Medicine 4:123-131. (IF not available)
  • McEvoy CR, Falmer AA, van Pittius NC, Victor TC, van Helden PD, Warren RM. (2007) The role of IS6110 in the evolution of Mycobacterium tuberculosis. Tuberculosis (Edinb). 87:393-404. (IF=3.425)
  • Michel AL, de Klerk LM, van Pittius NC, Warren RM, van Helden PD: (2007) Bovine tuberculosis in African buffaloes: Observations regarding Mycobacterium bovis shedding into water and exposure to environmental mycobacteria. BMC Vet. Res. 3:23. (IF not available)
  • Uys PW, Warren RM, van Helden PD. (2007) A Threshold Value for the Time Delay to TB Diagnosis. PLoS ONE Aug 22 2(1):e757. (IF not available)
  • Bourn W, Meyer Y, Stutz H, Warren RM, Williamson A, van Helden PD. (2007) Creation and characterisation of a high-copy-number version of the pAL5000 mycobacterial replicon. Tuberculosis (Edinb). 87:481-488. (IF=3.425)
  • Abdallah AM, Gey van Pittius NC, Champion PAD, Cox J, Luirink J, Vandenbroucke-Grauls CMJE, Appelmerk BJ, Bitter W. (2007) Type VII Secretion – Mycobacterium shows the way. Nat. Rev. Microbiol.  5:883-891. (IF=15.845)
  • Mitchison DA, Jindani A, Davies GR, Sirgel F (2007) Isoniazid activity is terminated by bacterial persistence. J. Infect. Dis. 195:1872-1872. (IF=5.363)
  • Todorov SD, Brink M, Guigas C, Schillinger U, Wiid I, Wachsman M, Holzapfel W, Dicks L (2007) Boza, a natural source of probiotic lactic acid bacteria. J. Appl. Microbiol. Aug 1 [Epub ahead of print] (IF=2.206)

Non Peer-Reviewed Articles (Total: 2)

  • Walzl G, Mistry R, Dockrell HM, van Helden PD, Cliff JM. 2007. Letter to the Editor. J. Infect. Dis. 196, 649-650. (Reply to Davies et. al. 2007, Biomarkers and surrogate end points in clinical trials of tuberculosis treatment. J Infec Dis. 196:649–650.) (IF=5.363)
  • Hanekom M, van der Spuy GD, van Pittius NC, McEvoy CR, Ndabambi SL, Victor TC, Hoal EG, van Helden PD, Warren RM. (2007) Letter to the Editor. Journal of Clinical Microbiology 45: 4092-4093 (Reply to Mokrouso I. and Narvskaya O. Designationof major mycobacterial interspersed repetitive-unit types within Mycobacterium tuberculosis Beijing genotype, an important point. Letter to the Editor. J. Clin. Microbiol. 45: 4092-4093.)(IF=3.445)

Published Abstracts (Total: 19)

  • Babb C, Nel H, van Helden PD, Hoal EG.  (2007) Rantes-403A promoter polymorphism is associated with TB in a South African coloured population. Int. J. Tuberc. Lung Dis. 11:S75.
  • Diacon AH, Kawasaki M, Venter A, van Helden PD, Donald PR, Matsumoto M. (2007) Ex-vivo activity of anti-tuberculosis agents in sputum from patients with untreated smear-positive. Int. J. Tuberc. Lung Dis. 11:S62.
  • Diacon AH, Patientia RF, Mclleron H, Smith P, Venter A, Maritz S, van Helden PD, Donald PR.  (2007) Early bactericidal activity and bioavailability of high dosage rifampicin in smear-positive pulmonary TB.  Int. J. Tuberc. Lung Dis. 11:S162.
  • Gey van Pittius NC, Warren RM, van Helden PD. (2007) Non-tuberculous mycobacteria (NTM) isolated from a low HIV community with a high incidence of tuberculosis. Int. J. Tuberc. Lung Dis. 11:S166.
  • Kruger C, Pheiffer C, Beyers N, Godfrey-Faussett P, Ayles H, van Helden PD, Lawrence K, Botha T. (2007) Non-tuberculous mycobacteria recovered during a prevalence survey in areas with high TB prevalence in RSA. Int. J. Tuberc. Lung Dis. 11:S69.
  • Uys PW, Warren RM, van Helden PD.  (2007) A threshold value for the time delay to TB diagnosis. Int. J. Tuberc. Lung Dis. 11:S109.
  • Victor T, Calver A, Falmer A, Strauss O, Streicher E, Louw G, Johnson R, Trollip A, Jordaan A, van Helden PD, Warren R. (2007) Molecular epidemiology of drug-resistant tuberculosis in areas of high HIV prevalence. Int. J. Tuberc. Lung Dis. 11:S48.
  • Hanekom M, McEvoy CRE, Gey van Pittius NC, van der Spuy GD, Ndabambi SL, Victor TC, van Helden PD, Warren RM. (2007) Population structure M. tuberculosis in different settings defines the usefulness of MIRU-VNTR typing. Int. J. Tuberc. Lung Dis. 11:S114.
  • Hanekom M, van der Spuy GD, Streicher E, Gey van Pittius NC, Ndabambi SL, McEvoy CRE, Kidd M, Hoal EG, Beyers N, Victor TC, van Helden PD. (2007) Beijing strains: increased pathogenecity in different hosts and different geographical settings. Int. J. Tuberc. Lung Dis. 11(11) S115.
  • C Johnson R, Warren R, Falmer A, Murray M, van Helden PD, Victor T.  (2007) Certain clusters within the M.tuberculosis Beijing lineage spread more readily. Int. J. Tuberc. Lung Dis. 11:S120
  • Louw GE, Warren RM, van Helden PD, Victor TC. (2007) Efflux pump inhibitors modulate rifampicin resistance in Mycobacterium tuberculosis. Int. J. Tuberc. Lung Dis. 11:S120.
  • Möller M, Kwiatkowski R, Nebel A, van Helden PD, Hoal EG, Schreiber S. (2007) Human genetic susceptibility to TB: the investigation of candidate genes influencing intereron gamma levels. Int. J. Tuberc. Lung Dis. 11: S74.
  • Mardarowicz IH, Wiid I, Pheiffer C, Warren R, van Helden PD. (2007) The Prevalence of airborne Mycobacterium tuberculosis in health care settings in a high incidence area. Int. J. Tuberc. Lung Dis.  11:S129.
  • Strauss OJ, Warren RM, Jordaan A, Streicher EM, Hanekom M, Falmer AA, Albert H, Trollip A, Hoosain E, van Helden PD. (2007) Spread of atypical MDR Beijing strains among re-treatment tuberculosis cases In Port Elizabeth, South Africa. Int. J. Tuberc. Lung Dis. 11:S121.
  • Streicher EM, Gey van Pittius NC, van der Spuy G, Dale JW, Beyers N, van Helden PD, Victor TC, Warren RM.  Towards the understanding the evolution of principle genetic group 2 strains of Mycobacterium tuberculosis.(2007) Int. J. Tuberc. Lung Dis. 11:S122.
  • Chaoui I, Sabouni R, Jordaan A, Kourout M, Akrim M, Elmzibri M, Victor TC. (2007) Mutations responsible for Mycobacterium tuberculosis drug resistance in Morocco.  Int. J. Tuberc. Lung Dis. 11:S265.
  • Mardarowicz IH, Wiid I, Pheiffer C, Warren R, van Helden PD, Diacon A, Botha T, Trao VT.  (2007) The prevalence of airborne Mycobacterium tuberculosis in health care settings in a high incidence area.  Int. J. Tuberc. Lung Dis. 11:S129.
  • Donald P, Cilliers K, Willemse M, Lebadarios D, Schaaf H, Maritz J, Werely C. (2007) Pyridoxine serum concentrations in children hospitalised with tuberculosis.  Int. J. Tuberc. Lung Dis. 11:S225.
  • Hesseling AC, Mandalakas AM, Chegou N, Kirchner HL, Zhu X, Black G, Stanley K, Beyers N, Marais BJ, Walzl G.  (2007) The impact of M.tuberculosis exposure, age and HIV infection on T-Cell assays to detect M. tuberculosis infection. Int. J. Tuberc. Lung Dis. 11:S225.

Technical Reports (Total: 1)

  • IAEA Progress report on drug resistance project for South Africa (SAF6008).

Products / Artifacts / Patents

Patents

  • A South African patent (PA137469/P2) with the title “Bacterial secretion system and uses therefor” has been filed on the 05th of June 2007, under the name of Nicolaas Claudius Gey van Pittius, Robin Mark Warren and Paul David van Helden.
  • A South African Provisional Patent Application (2007/06915) with the title “A mixing device” has been developed during this period and was filed on the 17 August 2007, under the name of Robin Mark Warren, Kim Gilberte Pauline Hoek, Nicolaas Claudius Gey van Pittius, Paul David van Helden and Thomas Calldo Victor
  • A South African Provisional Patent Application (PA144935/P) with the title “Method for the detection of DNA sequence polymorphisms” has been developed during this period and was filed on the 17 August 2007, under the name of Robin Mark Warren, Kim Gilberte Pauline Hoek, Nicolaas Claudius Gey van Pittius, Paul David van Helden and Thomas Calldo Victor
  • A South African Provisional Patent with the title “Method for the detection and identification of non-tuberculous mycobacteria (NTM)” has been developed during this period.  This application still needs to be filed under the name of Nicolaas Claudius Gey van Pittius, Robin Mark Warren, Kim Gilberte Pauline Hoek and Paul David van Helden
  • A United States Provisional Patent Application with the title “DNA Array Specific for the PE and PPE gene families within Mycobacterium spp.” was filed on the 07th August 2007 under the names of Mazza, A., Brousseau, R., Mardassi, H., Gey van Pittius, N.C.

Products

  • Fluorescent microscope power supply: We have developed a prototype of a power independent fluorescence microscope. This microscope can be used in all settings for the diagnosis of TB through O-Auramine staining. The microscope has the following features: LED fluorescence, Hand generated power supply, Solar power supply, Battery power supply and Mains compatibility.We were offered a free booth at the IUATLD International Conference to display this prototype microscope in Cape Town (Nov 2007) as part of the exhibits.  Development of this item is being pursued with Innovus (SU) and the MRC Innovation Centre. A grant application to Optimus Foundation (Switzerland) and the MRC is engaged in talks with a BEE partner. We think that this item could revolutionise TB sputum smear diagnostics, particularly in the developing world.

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 89)

  • Mizrahi V. Session Chair and Invited Attendee, HHMI Investigators Meeting, Howard Hughes Medical Institute, Chevy Chase, MD, 21-24 January 2007
  • Kana BD, Gordhan BG, Sung N, Downing KJ, Machowski EE, Matsoso LG, Tsenova L, Young M, Kaplan G, Mizrahi V. The Role of Resuscitation Promoting Factors in the Virulence of Mycobacterium tuberculosis. Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Mizrahi V. Report-back on the Workshop on Training in the TB Basic Sciences held in Cape Town in June 2006. Invited talk and Session Chair at the Columbia University-southern African Fogarty AITRP Reunion, CAPRISA, University of KwaZulu-Natal, Durban, 1-3 February, 2007
  • Kana BD. Fogarty Trainee Report-back. Invited talk Presented at the Columbia University-southern African Fogarty AITRP Reunion, CAPRISA, University of KwaZulu-Natal, Durban, 1-3 February, 2007
  • Matsoso LG. Fogarty Trainee Report-back. Invited talk Presented at the Columbia University-southern African Fogarty AITRP Reunion, CAPRISA, University of KwaZulu-Natal, Durban, 1-3 February, 2007
  • Kana BD, Gordhan BG, Sung N, Downing KJ, Machowski EE, Matsoso LG, Tsenova L, Young M, Kaplan G, Mizrahi V. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis. Poster presented at the Keystone Symposium on Tuberculosis: From Lab Research to Field Trials. Vancouver, Canada, 20-25 March, 2007.
  • Gordhan BG. Invited talk on research and training activities conducted as an MRC Career Development Awardee. MRC Career Development Launch, Cape Town, 23 March, 2007.
  • Kana BD. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis: Waking TB Up. Invited talk presented at the Department of Genetics, University of Pretoria, 12 April, 2007.
  • Kana BD, Gordhan BG, Sung N, Downing KJ, Machowski EE, Matsoso LG, Tsenova L, Young M, Kaplan G, Mizrahi V. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis. Invited talk presented at the American Society for Biochemistry and Molecular Biology Annual Meeting, Washington D.C. 28 April – 2 May.
  • Savvi S, Mizrahi V, Dawes SS. 2007. The methylmalonyl CoA pathway is central in the metabolism of Mycobacterium tuberculosis. Poster presented at the 2nd ASM Conference on Integrating Metabolism and Genomics (IMAGE2), Montreal, Canada, April 30 – May 3, 2007.
  • Warner DF, Savvi S, Mizrahi V, Dawes SS. 2007. A riboswitch regulated expression of the coenzyme B12-dependent methionine synthase in Mycobacterium tuberculosis: implications for differential methionine synthase function in strains H37Rv and CDC1551. Invited talk presented at the the 2nd ASM Conference on Integrating Metabolism and Genomics (IMAGE2), Montreal, Canada, April 30 – May 3, 2007.
  • Warner DF. Vitamin B12 metabolism in Mycobacterium tuberculosis. Seminar presented to the Department of Physiology, University of the Witwatersrand, 8 May 2007.
  • Gordhan BG. Ensuring effective health and safety management in the laboratory. Invited talk presented at the 3rd Annual Laboratory Management Conference, Johannesburg, 21-22 May, 2007.
  • Kana BD. Good laboratory practice and accreditation in laboratories. Invited talk at the 3rd Annual Laboratory Management Conference, Johannesburg, 21-22 May, 2007 [Voted as best talk of the conference]
  • Gordhan BG. Panelist, Addressing Global Trends and Directions for Laboratory Management, Panel Discussion held at the 3rd Annual Laboratory Management Conference, Johannesburg, 21-22 May, 2007.
  • Kana BD. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis: Waking TB Up. Seminar presented to the Department of Molecular Medicine and Haematology, University of the Witwatersrand, 23 May, 2007
  • Kana BD, Gordhan BG, Sung N, Downing KJ, Machowski EE, Matsoso LG, Tsenova L, Young M, Kaplan G, Mizrahi V. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis. Seminar presented to the School of Molecular and Cell Biology, University of the Witwatersrand, 24 July, 2007
  • Warner DF, Kana BD, Machowski EE, Sung N, Brackin R, Gordhan BG, Dawes SS, Stoker NG, Kaplan G, Mizrahi V. Mutational mechanisms in mycobacteria: Implications for the evolution of drug resistance. Invited talk presented at the joint X IUBMB Conference and XXXVI Annual Meeting of the Brazilian Society for Biochemistry & Molecular Biology (SBBq), Salvador da Bahia, Brazil, 21-25 May 2007.
  • Mizrahi V. Key scientific issues pertaining to multi- and extensively drug-resistant tuberculosis. Invited lecture, Darwin Seminar Series, Africa Genome Education Institute, University of Cape Town, 6 June 2007.
  • Warner DF. Vitamin B12 metabolism in Mycobacterium tuberculosis. Seminar presented to the Department of Molecular Medicine and Physiology, University of the Witwatersrand, 6 June 2007.
  • Kana BD, Gordhan BG, Downing KJ, Sung N, Vostroktunova G, Machowski EE, Shleeva MO, Young N, Kaprelyants A, Kaplan G, Mizrahi V. The role of resuscitation promoting factors in growth and resuscitation of Mycobacterium tuberculosis. Plenary Lecture, Festschrift in Honour of Professor Lafras M. Steyn, University of Cape Town, 28 June, 2007
  • Mizrahi V. Key scientific issues pertaining to multi- and extensively drug-resistant tuberculosis. Invited lecture, Molecular Medicine and Hamatology Seminar Series, University of the Witwatersrand Medical School, 1 August, 2007
  • Kana BD. The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis. Invited talk presented at the School of Medicine, University of Leicester, Leicester, UK, 31 August, 2007
  • Gordhan BG, Kana BD, Sung N, Downing KJ, Machowski EE, Young M, Kaplan G, Mizrahi, V.  The role of the five resuscitation-promoting factors in the growth and resuscitation of Mycobacterium tuberculosis. Poster talk presented at the 47th Interscience Conference on Antimicrobial Agents and Chemotherapy, Chicago, USA, 17-20 September, 2007. [Winner, ICAAC Program Committee Award in the area of Pathogenesis and Microbial Diseases].
  • Kana BD, The role of resuscitation promoting factors in the virulence of Mycobacterium tuberculosis; Waking TB Up. Seminar presented at the Council for Scientific and Industrial Research, Modderfontein, 21 September, 2007
  • Mowa MB, Kana BD, Dawes SS, Kaplan G, Mizrahi, V. The role of alternate small subunits of class I ribonucleitide reductase in Mycobacterium tuberculosis. Poster presented at the BacPath9 Conference, Lorne, Victoria, Australia, 23-26 September, 2007.
  • Dawes SS. Identification of a vitamin B12 transporter of Mycobacterium smegmatis. Talk presented at the New Zealand TB Meeting, Malaghan Institute, Wellington, New Zealand, 8 October, 2007.
  • Mizrahi V. Key scientific issues pertaining to multi- and extensively drug-resistant tuberculosis. Invited lecture, Human Genetics Seminar Series, National health Laboratory Service & University of the Witwatersrand, 11 October 2007.
  • Ahmadou Ahidjo B, Machowski EE, Gordhan BG, Mizrahi, V. Functional characterization of VapBC toxin-antitoxins in mycobacteria. Talk presented at the MRC Research Day, MRC, Cape Town, 18 October, 2007.
  • Goosens V, Mizrahi V, Gordhan BG. The construction and phenotypic characterization of mycobacterial mutants defective in DNA glysolyases. Poster presented at the MRC Research Day, MRC, Cape Town, 18 October, 2007.
  • Gordhan BG. Ensuring Health and Safety Standards and Management in the Laboratory. Invited talk presented at the Laboratory Management Symposium, Johannesburg, 24-25 October, 2007.
  • Mizrahi V. Survival and Subversion Strategies of Mycobacterium tuberculosis: Lessons from Resuscitation and Genetic Adaptation to Stress. Inaugural Lecture, University of the Witwatersrand, 29 October, 2007
  • Babb C, van der Merwe L, Beyers N, Pheiffer C, Walzl G, Duncan K, van Helden P, Hoal E.
    Vitamin D receptor gene polymorphisms and sputum conversion time in pulmonary tuberculosis patients. Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Mitchell J, Gey van Pittius N, Warren R, Gomez J, McKinney JD, van Helden P, Ryffel B, Botha T. Reinfection dynamics of Mycobacterium tuberculosis in a THP1 human macrophage cell line. Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Gey van Pittius NC, Warren RM, van Helden PD. Non-tuberculous Mycobacteria (NTM) Isolated from a low HIV Community with a High Incidence of Tuberculosis. Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Hayward D, Kenyon C, van Helden P, Wiid I.  Unique distribution of glutamine synthetase in pathogenic mycobacteria.  Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Möller M, Kwiatkowski R, Nebel A, van Helden P, Hoal EG, Schreiber S.  Allelic variation in BTNL2 and susceptibility to tuberculosis in a South African population.  Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Streicher EM,  Gey van Pittius NC, van der Spuy GD, Beyers N, Dale JW, van Helden PD, Victor  TC, Warren RM. Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Hanekom M, van der Spuy GD, Streicher E, Gey van Pittius NC, Ndabambi SL, McEvoy CRE, Kidd M, Hoal EG, Beyers N, Victor TC, van Helden PD, Warren RM. Sublineages of the Mycobacterium tuberculosis Beijing strain family show increased ability to spread and cause disease in different host populations from different geographical settings.  Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Johnson R, Warren RW, Falmer AA, Strauss J, Cloete K, Murray M, Young D, van Helden PD, Victor TC. Cluster 220 is dominant amongst Beijing drug resistant strains in the Western Province of South Africa.  Poster presented at the Keystone Symposium on Tuberculosis: from Lab Research to Field Trials, Vancouver, Canada, 20-25 March 2007.
  • Warren RM. Lessons Learnt in Research. Invited speaker to the MRC World TB Day Programme, Cape Town, South Africa, 23 March 2007.
  • Van Helden PD. MDR and XDR TB: Invited speaker to the “Recrudescence of an old disease: Tuberculosis" Advanced training course, University of Naples, Naples, Italy, 02-05 May 2007.
  • Hoal EG. Human genetic susceptibility to tuberculosis: Invited speaker to the “Recrudescence of an old disease: Tuberculosis" Advanced training course, University of Naples, Naples, Italy, 02-05 May 2007.
  • Hoal EG, van Helden PD, Moller M. Alleleic variation in BTNL2 and susceptibility to tuberculosis in a South African population. EMBO conference on Genetics and Mechanisms of Susceptibility to Infectious diseases. Pasteur Institute, Paris, France, 21-24 November 2007.
  • Van Helden PD. The management of research institutes: Problems and challenges. Invited speaker to CREST/CHE Research Directors Forum, Somerset West, Cape Town, South Africa, 30-31 May 2007.
  • Warren RM. The epidemiology of M. tuberculosis strains with the Beijing genotype: a South African perspective Invited speaker to the TB in the 21st Century Consortium Meeting, Mexico City, Mexico, 2007.
  • Warren RM. Research at the Centre for Molecular and Cellular Biology. Invited speaker to PEPFAR –MRC, 2007.
  • Gey van Pittius NC. Comparative Genomics of M. tuberculosis Complex with a focus on variability of the PE-PPE families. Invited speaker at mini-symposium: “Role Of PE/PPE Gene In The Biology Of M. tuberculosis And Their Potential Role In Immune Evasion In Tuberculosis”, Division of International Medicine & Infectious Diseases,  Department of Medicine, Weill Medical College of Cornell University, New York, United States of America, March 2007.
  • Gey van Pittius NC. Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families. Invited presentation at the MRC/NHLS/WITS Molecular Mycobacteriology Research Unit and DST/NRF Centre for Biomedical TB Research, National Health Laboratory Service, Johannesburg, South Africa, 25 June 2007.
  • Gey van Pittius, NC. WOTRO Project Development Workshop, University of Stellenbosch, Tygerberg, South Africa.
  • Streicher EM, Dely C, Bester M, van Helden PD, Warren RM, Victor TC. XDR-TB in the Western Cape province of South Africa. Invited speaker to the South African Medical Research Council Research Day, Cape Town, South Africa, 18 October 2007.
  • Nene N, Gey van Pittius NC, Walzl G, Stanley K, van Helden PD, Black GF. Immunity to tuberculous and non-tuberculous mycobacteria in adult household contacts of Tb cases in the Western Cape.
  • Möller M, Kwiatkowski R, Nebel A, Van Helden PD, Hoal EG, Schreiber S. Human genetic susceptibility to tuberculosis: the investigation of candidate genes influencing interferon gamma levels. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • van Helden PD, Gey van Pittius NC, Warren RM, Michel A, Hlokwe T, Morar D, Godfroid J, Du Plessis EC, Bengis R. Pulmonary infection due to mycobacterium goodii in a spotted hyaena (crocuta crocuta) from South Africa. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Calver A, Falmer AA, Warren RM, Strauss OJ, Liversage T, Masibi MH, Victor TC, van Helden PD. Emergence of extensively drug resistant (XDR) tuberculosis in a well functioning control program. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Parsons SDC, Gous TA, Streicher EM, Gey van Pittius NC, Warren RM, van Helden PD. Mycobacterium tuberculosis infection in a stray dog. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Louw GE, Warren RM, van Helden PD, Victor TC. Efflux pump inhibitors modulate rifampicin resistance in Mycobacterium tuberculosis. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Truter E, Babb C, Nel H, van Helden PD, Hoal EG. Genetic susceptibility to tuberculosis: a candidate gene association study on rantes (CCL5). Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Hoek KGP, Warren RM, Walzl G, Bezuidenhout J, Aguilar LD, Orozco EH, Hernández Pando R, van Helden PD. In situ hybridisation vs in situ PCR in the study of Mycobacterium tuberculosis. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Johnson R, Warren R, Falmer A, Murray M, van Helden PD, Victor T. A sub lineage of the drug resistant beijing strain has a higher propensity to transmit and cause drug-resistant Tb. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Hanekom M, van der Spuy GD, Streicher E, Gey van Pittius NC, Ndabambi SL, Mcevoy CRE, Kidd M, Hoal EG, Beyers N, Victor TC, van Helden PD, Warren RM. Sublineages of the mycobacterium tuberculosis beijing strain family show increased ability to spread and cause disease in different host populations from different geographical settings. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Babb C, Van Der Merwe L, Beyers N, Pheiffer C, Walzl G, Duncan K, van Helden PD, Hoal EG. Vitamin D receptor gene polymorphisms and sputum conversion time in pulmonary tuberculosis patients. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Hayward D, Kenyon C, van Helden PD, Wiid I. Mycobacterium tuberculosis glutamine synthetase modification and export . Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Robinson ZL, van Helden PD, Gey van Pittius NC. Antigenicity of M. smegmatis esat-6. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Kruger C, Botha T, Beyers N, Van Helden P, Ayles H, Godfrey-Faussett P, Pheiffer C. Non-tuberculous mycobacteria recovered during a prevalence survey in areas with a high Tb prevalence in South Africa. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • McEvoy CRE, Gey van Pittius NC, van Helden PD, Warren RM. A method for the enrichment of m. tuberculosis genomic fragments containing 5-methylcytosine. Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Mitchell J, Gey van Pittius NC, Warren R, van Helden PD, Botha T. Reinfection dynamics of Mycobacterium tuberculosis in a THP1 human macrophage cell line.  Poster presented at the 51st Academic Year Day, University of Stellenbosch, 15 August 2007.
  • Hayward D, Wiid I, van Helden PD, Kenyon C. Mycobacterium tuberculosis glutamine synthetase modification and export. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa. 8-12 November 2007.
  • Hanekom M, McEvoy C.R.E., Gey van Pittius N.C., van der Spuy G.D., Ndabambi S.L., Victor T.C, van Helden P.D., Warren R.MStructure of M. tuberculosis in different settings defines the usefulness of MIRU-VNTR typing. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Hanekom M, van der Spuy GD, Streicher E, Gey van Pittius NC, Ndabambi SL, McEvoy CRE, Kidd M, Hoal EG, Beyers N, Victor TC, van Helden PD, Warren RM. Beijing Strains: increased pathogenicity in different hosts and different geographical settings. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa. 8-12 November 2007.
  • Strauss OJ, Warren RM, Jordaan A, Streicher EM, Hanekom M, Falmer AA, Albert H, Trollip A, Hoosain E, van Helden PD, Victor TC. Spread of atypical MDR Beijing strains among retreatment tuberculosis cases in Port Elizabeth, South Africa. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Uys P. Delays in diagnosing TB: Causes and consequences. Talk presented at the 38th Union World Conference on Lung Health. Cape Town, South Africa, 8-12 November 2007.
  • Hesseling AC, Mandalakas AM, Marais BJ, Chegou NN, Les Kirchner H, Zhu X, Black GF, Stanley K, Beyers N, Walzl G. ESAT-6: A highly sensitive marker of recent mycobacterium tuberculosis infection. Invited speaker to the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Victor TC, Calver A, Falmer A, Strauss O, Streicher E, Louw G, Johnson R, Trollip A, Jordaan A, van Helden PD, Warren R. Molecular epidemiology of Drug resistant tuberculosis in areas of high HIV prevalence. Invited speaker to the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Streicher EM, Gey van Pittius NC, van der Spuy G, Dale JW, Beyers N, van Helden PD, Victor TC, Warren RM. Towards the understanding the evolution of principle genetic group 2 strains of Mycobacterium tuberculosis.  Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Shean KP, Siwendu SN, Victor TC, Jordaan AM, Willcox PA. Multidrug-resistant tuberculosis (MDR-TB) outbreak investigation, in the Western Cape Province, South Africa. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Chaoui I, Sabouni R, Jordaan A, Kourout M, Akrim M, Elmzibri M, Victor TC. Mutations responsible for Mycobacterium tuberculosis drug resistance in Morocco. Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Louw GE, Warren RM, van Helden PD, Victor TC. Efflux pump inhibitors modulate rifampicin resistance in Mycobacterium tuberculosis.  Poster presented at the 38th Union World Conference on Lung Health, Cape Town, South Africa, 8-12 November 2007.
  • Warren R.M., Hoek K.G.P., Moolman-Smook H,  Carelse-Tofa K, Jordaan A, van der Spuy GD, Streicher EM, Victor TC, Gey van Pittius N.C., van Helden P.D. Rapid, Reliable and Easy Fluorometric Assay for Susceptibility testing of Rifampicin in Mycobacterium tuberculosis (FAST-RIF). Invited speaker to the South African Medical Research Council Research Day, Cape Town, South Africa, 18 October 2007.
  • Mandalakas AM, Hesseling AC, , Chegou NN, Les Kirchner H, Zhu X, Marais BJ, Stanley K, Beyers N, Black GF,  Walzl G. High level of discordant IGRA results in HIV-infected adults and children. Poster presented at the MSF- One day symposium on TB field diagnostics, Cape Town, South Africa, 07 November 2007.
  • Black GF, Thiel B, Walzl G, Boom H, Hill PC, Mayanja H, Stanley K, Chervenak K,  Franken K, Friggen A, Klein MR, Ota M, Ottenhoff THM, Parida S, Kaufamnn SHE, and the GC#6-74 Consortium. Immunogenicity testing of new potential diagnostic antigens  of Mycobacterium tuberculosis infection by whole blood IFN-g release assay in 3 distinct African populations. Poster presented at the MSF- One day symposium on TB field diagnostics, Cape Town, South Africa, 07 November 2007.
  • Walzl G, Black GF, Stanley K, Loxton A, Chegou NN, Golakai HJ, Nene N,  Roberts T, Beyers N, Franken K, Friggen A, Ottenhoff THM, Klein M, Thiel B, and the GCGH Biomarkers for TB Consortium. Immunogenicity testing of new potential vaccine targets against Mycobacterium tuberculosis  by whole blood assay in a population with high TB prevalence. Poster presented at the British Society of Immunology Conference. Glasgow, Scotland, 2007.
  • Walzl G. Immune tests for TB. Invited talk to the FIDSSA Conference, Spier Cape Town, 29-31 October 2007.
  • Walzl G. Stop TB Working Group on New TB Drugs: Biomarkers for TB treatment response. Invited talk to the ICC, Cape Town, 13 November 2007.
  • Walzl G. Stop TB Working Group on New TB Drugs: Bio banking. Invited talk to the Cullinan Hotel, Cape Town, 9 November 2007.
  • Nene N, Gey van Pittius NC, Walzl G, Stanley K, van Helden PD, Black GF..Immunity to tuberculous and non-tuberculous Mycobacteria in adult household contacts of TB Cases in the Western Cape. Invited speaker to the South African Medical Research Council Research Day, Cape Town, South Africa, 18 October 2007.
  • Warren RM, Streicher EM, Calver A, Mlambo C, Marais E, Falmer A, Stolk M, van Helden PD, Victor TC. XDR-TB: Cause for concern or over-reaction?  Centre for Infectious Diseases: Expert Opinion on Infectious Diseases Symposium, Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa, November 2007.
  • Loxton A. Attended: Keystone Symposia 2007 on Immunological memory 3-8 March 2007, Sante Fe (New Mexico, USA), Received: Global Health Travel Award
  • Uys P. Using Mathematical Models to understand TB Epidemics. Invited seminar at SACEMA, 2007.

Conferences / Meetings Hosted

  • Prof Mizrahi served as Chair of the 2007 Gordon Research Conference on Tuberculosis Drug Development, Magdalen College, Oxford, United Kingdom, 26-31 August 2007.
  • Prof G Walzl and Dr. Gillian Black were the co-hosts of the Annual meeting of the Bill and Melinda Gates Foundation sponsored GC6-74 project, which was held in Cape Town during October 2007,

Other Relevant Outputs (including honors and awards to staff)

  • Prof. Mizrahi was awarded the Order of the Mapungubwe: Silver from President Thabo Mbeki on 21 September, 2007. This award was given in recognition of excellent contributions in biochemistry and molecular biology and for contributing to the development of genetic tools for the validation of drug targets and vaccine candidates for tuberculosis.
  • Prof. Mizrahi served on the Jury for the 2008 L’ORÉAL-UNESCO For Women in Science Awards in France. 
  • Prof. Mizrahi was appointed to a three-year term on the Council of Scientific Advisors of the International Centre for Genetic Engineering and Biotechnology (ICGEB) from January 2008. She is the only South African and one of only two Council members from Africa.
  • Dr. Gordhan received an Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC) Program Committee Award in the area of Pathogenesis of Microbial Diseases. The award recognised the outstanding quality of her abstract and was one of six selected from a total of 1878 accepted abstracts. The award was presented to Dr. Gordhan at the 47th Annual ICAAC conference in Chicago in Sept. 2007.
  • Dr. Kana was awarded a Bill & Melinda Gates Global Travel Award to attend the Keystone Symposium on Tuberculosis: From Lab Research to Field Trials, Vancouver, 20-25 March 2007.
  • Dr. Gordhan’s Career Development Award from the MRC was renewed for a final (fourth) year.
  • Dr. Andreas Diacon, conducting clinical trials, received the SU Rector’s award for Excellence in Research.
  • Prof. Valerie Corfield received an award for the best scientific exhibition at the 51st Academic Year Day of the University of Stellenbosch in August 2007.
  • Development of a spin-off company affiliated with the CBTBR – Task Applied Science, cc.
    Owner & full time employee, Dr. A. Diacon, pulmonologist. Task Applied Science currently employs 1 F/T & 2 P/T clinicians, 3 nursing sisters, 1 F/T & 1 P/T clerical staff, 1 driver, 4 medical technologists and pays 1 specialist medical consultant fees. For 2008, it is possible that the number of paid staff will increase.
2006

Books / Chapters in Books

  • Warner DF, and Mizrahi V. 2006. M. tuberculosis Physiology. In: Tuberculosis Handbook, Vol. 1 (Kaufmann SHE & Rubin EJ, eds.), Wiley VCH. Submitted (invited book chapter).
  • Chihota VN, van Helden PD, Victor TC, and Warren RM. 2006. Molecular Epidemiology of Tuberculosis in South Africa:  Lessons learnt. In Molecular Epidemiology and Population Genetics of Tuberculosis, Eds. Academy of Science, Malaysia. pp 69-100.
  • van Helden PD, Moller M, Babb C, Warren RM, Walzl G, Uys P, and Hoal EG. 2006. TB epidemiology and Human Genetics. In Innate immunity to pulmonary infection. Wiley, Chichester (Novatis Foundation Symposium 279) pp 17-41.

Articles in peer-reviewed journals

Non peer-reviewed articles

Technical Reports

  • To IAEA ( Int Atomic Energy Agency): Report RAF/6.025-004:   Regional progress report on training, data Management and Quality assurance programmes on radionuclide based molecular techniques in the detection of drug resistant tuberculosis –  24 – 28 April 2006,  Addis Ababa, Ethiopia.
  • To IAEA ( Int Atomic Energy Agency): Final report RAF 6025: Isotopes for the control of drug resistant TB (Nairobi May 2006, 8 different African countries).

Products / Artifacts / Patents

  • Warren R, Gey van Pittius N, van Helden P. 2006 Single step method for the diagnosis and speciation of M tuberculosis, M africanum, M bovis and M bovis BCG. SA Provisional Patent 2006/00683.

Conferences / meetings attended

  • Gordhan, B.G. Effectively Managing the Waste Process - Handling, Storage, Transport and Disposal. Talk presented at Laboratory Management for Improved Performance conference, Park Hyatt, Johannesburg, 17-18 May 2006.
  • Mizrahi V. Chair/ Co-Organiser and Lab Head presenter, Workshop on Training in the TB Basic Sciences, Cape Town, 5-6 June 2006.
  • Dawes SS. CU-SA Fogarty AITRP Trainee feedback presentation, Workshop on Training in the TB Basic Sciences, Cape Town, 5-6 June 2006.
  • Kana, BD. CU-SA Fogarty AITRP Trainee feedback presentation, Workshop on Training in the TB Basic Sciences, Cape Town, 5-6 June 2006.
  • Mizrahi V. Beckman-Coulter Gold Medal Lecture, XXth Conference of the SA Society for Biochemistry and Molecular Biology, Pietermaritzburg, 2-5 July 2006
  • Gordhan BG, Smith DA, Kana BD, Bancroft G, Mizrahi, V. The carbon starvation-inducible genes Rv2557 and Rv2558 of Mycobacterium tuberculosis are not required for long-term survival under carbon starvation and for virulence in SCID mice. Talk presented at the XXth Conference of the SA Society for Biochemistry and Molecular Biology, Pietermaritzburg, 2-5 July 2006
  • Kana BD, Moon S, Warner DF, Gordhan BG, Machowski EE, Sacchettini J, Stoker NG, Kaplan G, Mizrahi V. Characterisation of Y-family DNA polymerases from Mycobacterium tuberculosis. Talk presented at the XXth Conference of the SA Society for Biochemistry and Molecular Biology, Pietermaritzburg, 2-5 July 2006
  • Mowa MB, Dawes SS, Mizrahi, V. Function of class Ic ribonucleotide reductase in Mycobacterium smegmatis. Poster presented at the XXth Conference of the SA Society for Biochemistry and Molecular Biology, Pietermaritzburg, 2-5 July, 2006
  • Savvi S, Mizrahi V, Dawes SS. Cobalamin dependent metabolic pathways and their contribution to virulence of Mycobacterium tuberculosis. Poster presented at the XXth Conference of the SA Society for Biochemistry and Molecular Biology, Pietermaritzburg, 2-5 July 2006
  • Warner DF, Machowski EE, Brackin R, Stoker NG, Boshoff HIM, Dawes SS, Mizrahi, V. Y-family polymerases in mycobacteria: roles in genetic adaptation and long-term survival. Invited talk presented at the 2006 Health Sciences Research Day, University of the Witwatersrand, 23 August, 2006. Winner: Best Oral Presentation Prize (Molecular and Cell Biology & Evolutionary Sciences section).
  • Warner DF, Savvi, S., Mizrahi, V., and Dawes, S.S. Vitamin B12-mediated inhibition of growth of Mycobacterium tuberculosis CDC1551. Poster presented at the Health Sciences Research Day, University of the Witwatersrand, 23 August 2006.
  • Mizrahi, V. The application of genetic tools to investigate molecular mechanisms of pathogenesis in Mycobacterium tuberculosis. Invited lecture presented at the EMBO/FEMS Spetsai Summer School on the Molecular Basis of Bacterial Virulence and Survival within Infected Hosts and in the Environment, Spetses, Greece, 4-15 September 2006
  • Mizrahi, V. Mechanisms of genetic adaptation in Mycobacterium tuberculosis: Implications for inter-strain variation and the evolution of drug resistance. Invited lecture presented at the EMBO/FEMS Spetsai Summer School on the Molecular Basis of Bacterial Virulence and Survival within Infected Hosts and in the Environment, Spetses, Greece, 4-15 September 2006
  • Warner DF, Machowski EE, Brackin R, Stoker NG, Boshoff HIM, Dawes SS, Mizrahi, V. Y family polymerases in mycobacteria: roles in genetic adaptation and long-term survival. Poster presented at the EMBO/FEMS Spetsai Summer School on the Molecular Basis of Bacterial Virulence and Survival within Infected Hosts and in the Environment, Spetses, Greece, 4-15 September 2006. Winner: Molecular Microbiology Best Poster Prize
  • Kana BD, Gordhan BG, Downing KJ, Sung N, Machowski EE, Matsoso LG, Shleeva M, Young M, Kaplan G, Kaprelyants AS, Mizrahi V. The role of resuscitation promoting-factor-like proteins in growth and resuscitation of Mycobacterium tuberculosis. Invited lecture presented at the International Scholars Meeting of the Howard Hughes Medical Institute, Janelia Farm Research Campus, VA, USA, 26-30 September, 2006
  • Savvi S, Mizrahi V, Dawes SS. Methylmalonyl CoA mutase (MCM) plays a nonredundant role in the metabolism of Mycobacterium tuberculosis. Talk presented at the Molecular and Cell Biology Group Symposium, University of the Witwatersrand, 5 October 2006.
  • Gordhan BG, Smith D, Kana BD, Bancroft G, Mizrahi, V. The carbon starvation-inducible genes Rv2557 and Rv2558 of Mycobacterium tuberculosis are not required for long-term survival under carbon starvation and for virulence in SCID mice. Talk presented at the Molecular and Cell Biology Group Symposium, University of the Witwatersrand, 5 October 2006.
  • Mizrahi, V. TB Research in the Post-Genomic Era. Invited lecture presented at Pandora’s Box: Implications of the Human Genome for Ordinary South Africans, Joint Faculty of Health Sciences and Health Graduates Association Symposium, University of the Witwatersrand, 25 November, 2006.
  • Gordhan BG, Boshoff HIM, Kana BD, Warner, Matsoso LG, Dawes SS, Downing KJ, Mizrahi, V. The application of genetic tools to investigate the molecular mechanisms of pathogenesis in Mycobacterium tuberculosis. Plenary lecture presented at the NICD Academic Day in Honour of the Official Opening of the NICD, National Institute for Communicable Diseases, Johannesburg, South Africa, 27-28 November, 2006.
  • van Helden PD. Antimicrobial Resistance in TuberculosisInternational Conference on Emerging Infectious Diseases. 19-22 March 2006. Atlanta, Georgia.
  • van Helden PD. Surrogate Markers to predict the outcome of anti-tuberculosis therapy. Third Annual Forum, EDCTP, 8-11 October 2006, Stockholm, Sweden.
  • Warren RM. TB Diagnosis: Past, Present and Future. Invited speaker TB day - 2 Military Hospital, March 2006.
  • Warren RM. PCR amplification. Invited speaker NHLS training on PCR, October 2006.
  • Warren RM. Role and Value of Molecular Investigation in TB Drug Resistance Surveys. Invited speaker at XDR-TB workshop – Johannesburg, September 2006.
  • Warren RM. Beijing in South Africa. TB in the 21st Century Consortium Meeting – Hanoi, Vietnam, February 2006.
  • Warren RM. Unlocking Epidemiological Secrets Through Comparative Genomics. Talk presented at the Bergen University, Norway, June 2006.
  • Gey van Pittius NC. Comparative Mycobacterial Genomics: uncovering the secrets of the genus Mycobacterium. Invited speaker, Department of Medical Microbiology, Vrije Universiteit Medical Centre, Amsterdam, Netherlands, April 2006.
  • Gey van Pittius NC. Environmental mycobacteria in a Western Cape population. Talk presented at the Cape Town TB Consortium Joint Symposium, Cape Town, March 2006.
  • Gey van Pittius NC. Non-tuberculous Mycobacteria (NTM) Isolated from a Community with a High Incidence of Tuberculosis: a Retrospective Study. 50th Academic Year day of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2006.
  • Gey van Pittius NC. Non-tuberculous Mycobacteria (NTM) Isolated from a Community with a High Incidence of Tuberculosis: a Retrospective Study”. Invited Oral Presentation, Royal Society/NRF Workshop on Cell wall lipids in Tuberculosis, Pretoria, South Africa, 29-30 June, 2006.
  • Gey van Pittius NC. Non-tuberculous Mycobacteria (NTM) Isolated from a Community with a High Incidence of Tuberculosis: a Retrospective Study”. Invited Oral Presentation at the XXth Conference of the South African Society of Biochemistry and Molecular Biology (SASBMB), Pietermaritzburg, South Africa, 2-5 July 2006
  • Victor TC, van Helden PD.TB Workshop with Department of Health and World Health Organisation, Johannesburg,  17-18 October 2006
  • Victor TC, van Helden PD. Workshop on Developing Demonstration Projects for Rapid Rifampicin Resistance Diagnostic Tools, Farm Inn Pretoria, 16 November 2006
  • Pheiffer C. Invited to the “Consortium to Respond Effectively against the Aids and Tuberculosis Epidemic” (CREATE) Investigator’s meeting held at Kwa Maritane Game Lodge in the North West Province, 18-19 September, 2006.
  • Gey van Pittius NC, van Helden PD. Invited talks, Livestock Health and Production Group Conference, Stellenbosch, August 2006
  • Uys PW, Warren R, van Helden PD, Murray M, Victor TC. The potential of rapid diagnosis for controlling drug-susceptible and drug-resistant TB in high prevalence communities. Poster presented at the TB and HIV Modelling Conference, Stellenbosch, November 2006.
  • Todorov SD, Wachsman MW, Wiid I, Brink M, Dicks LMT, Boza. A cereal-based beverage from Bulgaria is a source of probiotic lactic acid bacteria. Poster presented at the1st International symposium “Food, Veterinary and Medical Applications of Antimicrobial Peptides”. June 21st - 23rd 2006, ENITIAA, Nantes, France. Book of Abstracts, Section “Food and Medical Applications”, poster D-14, page 132.
  • Roberts T, Walzl G. Immune suppression of PBMC’S during active TB is restored by co-culture with cells obtained at treatment end. Poster presented at the1st joint meeting of European National Society of Immunology, Paris, France, 6-9 September 2006
  • Walzl G, Black GF, Stanley K, Loxton A, Chegou N, Golakai H, Nene N, Roberts T, Beyers N, Franken K, Friggen A, Ottenhoff T, Klein M and the Biomarkers4TB Consortium. Immunogenicity testing of new potential vaccine targets against Mycobacterium tuberculosis by whole blood assay in a population with high TB prevalence. TBV- TB vaccines for the World, 19-21 April 2006, Vienna, Austria.
  • Djoba Siawaya JF, Bapela NB, Veenstra H, Kidd M, Beyers N, van Helden P, Walzl G. Combined Immune Parameters and X-Ray Data in Early Prediction of Antituberculosis Therapy Response. Poster presented at the European Biomarkers Summit, Prague, Czech Republic, 26-27 September 2006
  • Hanekom M. Piecing Together The Evolutionary Puzzle Of The Mycobacterium Tuberculosis Beijing Strain Family. Poster presented at XXth Conference of the South African Society of Biochemistry and Molecular Biology (SASBMB), Pietermaritzburg, South Africa, July 2006.
  • Warren RM. Positive Association Between Site Of Disease And Mycobacterium Tuberculosis Strain In Children. Poster presented at the 37th Union World Conference on Lung Health of the International Union Against Tuberculosis and Lung Disease (IUATLD), Paris, France, 31 October - 4 November 2006
  • Mahasha PW, Machowski EE, Warren RM, van Helden PD, Gey van Pittius NC. The trafficking of the Mycobacterium tuberculosis PE and PPE proteins. Poster presented at the27th African Health Sciences Conference, Durban, South Africa, 3-7 December 2006

Conferences / meetings hosted

  • Prof. Mizrahi served as Chair and Co-Organiser of the Workshop on Training in the TB Basic Sciences, Vineyard Hotel, Cape Town, 5 June 2006.
  • In March 2006, the SU node co-hosted the Cape Town TB Consortium Joint Symposium with the Institute for Infectious Diseases and Molecular Medicine (IIDMM) from UCT. This one day symposium included 4 sessions and covered topics related to the epidemiology of TB, Public Health, immunology and clinical aspects of tuberculosis research. Profs. Walzl and Warren chaired two of the sections and seven oral presentations were given by members of the CBTBR. Presentations included results from research on M. tuberculosis evolution, environmental mycobacteria in the Western Cape population, allelic variations and TB susceptibility and new approaches in TB drug evaluation.

Other Relevant Outputs (including honors and awards to staff)

  • Development of a spin-off company affiliated with the CBTBR – Task Applied Science, cc.
    Owner and full time employee, Dr. A. Diacon, pulmonologist. Task Applied Science currently employs 1 F/T and 2 P/T clinicians, 3 nursing sisters, 1 F/T and 1 P/T clerical staff, 1 driver, 4 medical technologists and pays 1 specialist medical consultants fees. For 2007, it is possible that the paid staff will increase by a further 6-7 persons and support for a clinical fellow is also planned.
  • Prof. Mizrahi was awarded the Gold Medal of the South African Society for Biochemistry and Molecular Biology (SASBMB), which she received at the biannual conference of the SASBMB in Pietermaritzburg in July 2006. The Gold Medal is awarded in recognition of lifetime achievements in research and capacity development in the fields of biochemistry and molecular biology in the field. Prof. Mizrahi is the first woman to receive this medal.
  • Prof. Mizrahi was awarded the DST Distinguished Woman Scientist Award for 2006 from the Department of Science and Technology. This award forms part of the annual DST Women in Science awards and is awarded to a senior woman scientist who has made significant contributions to the development of Science and Technology in South Africa.
  • Prof. Mizrahi won the Shoprite-Checkers/SABC2 2006 Woman of the Year award in the category of Science & Technology.
  • Prof. Warren was awarded the 2006 Stellenbosch University Rector’s Award for Excellence in Research
  • Prof. Mizrahi was appointed to the Scientific Advisory Committee of the EU-funded New Medicines for TB Program (NM4TB), which is based at the Institut Pasteur (Paris). She was also appointed to a three-year terms on the Committee on Genomics and Discovery of the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR).
  • Prof. Mizrahi was appointed to the Editorial Advisory Panel of the Biochemical Journal, the flagship journal of the Biochemical Society (UK), with an impact factor of 4.224. She is one of two panel members from South Africa
  • Dr. Gordhan’s Career Development Award from the MRC was renewed for a third year.
  • Dr. Kana received a Global Health Travel Award to attend the Keystone Conference on Tuberculosis, 20-24 March 2007, Vancouver, Canada.
  • Profs. van Helden and Walzl were invited to attend special panel meeting of GATB in December to discuss biomarkers
  • Dr. Gey van Pittius was appointed to a grants committee of the NRF
  • Dr. F. Sirgel was contracted by the Global Alliance for TB Drug Development to assist in assessing potential clinical trial sites for the evaluation of candidate anti-tuberculosis medication (May to December 2006).

Software Developed

  • A visual basic/MySQL-based results, patient management and sample management database system for the Gates GC-674 international collaborative project. The system stores and validates laboratory results and clinical report data, tracks the storage and usage of clinical samples and schedules follow-up visits.
  • A visual basic/MySQL-based database system for managing TB patients and their clinical samples and data at a remote clinic site in Lawaaikamp, George. The system uses fingerprint biometrics to identify patients' and track their clinic visits. Sputum samples are barcoded and tracked as they are processed from the clinic, through the NHLS, and then to the molecular lab at Tygerberg. Lab results are uploaded directly to the clinic via ADSL.
  • Ongoing development of a visual basic/MS access-based database used for recording and analysing the clinical and laboratory results of a number of TB projects.
  • Ongoing development of a PHP/MySQL-based DNA sequencer online job submission and management system used for tracking job status, accounting and statistical analysis of sequencer throughput.
2005

JOURNAL PUBLICATIONS

  1. Downing KJ, Mischenko VV, Shleeva MO, Young D, Young M, Kaprelyants AS, Apt AS, Mizrahi V. 2005. Mutants of Mycobacterium tuberculosis lacking three of the five rpf-like genes are defective for growth in vivo and for resuscitation in vitro. Infect. Immun. 73:3038-3043.

  1. Gey van Pittius NC, Sampson SL, Warren RM, van Helden PD. 2005. Genome variation in Mycobacterium tuberculosis: pathogens genomics. S. Afr. J. Sci. 100:465-470.

  2. Kaufmann SHE, Cole ST, Mizrahi V, Rubin E, Nathan, C. 2005. Mycobacterium tuberculosis and the host response. J. Exp. Med. 201:1693-1697.

  1. Loots DT, Wiid IJ, Page BJ, Mienie LJ, van Helden PD. 2005. Melatonin prevents the free radical and MADD metabolic profiles induced by antituberculosis drugs in an animal model. J Pineal Res. 38:100-6.

  2. Machowski EE, Dawes SS, Mizrahi V. 2005. TB tools to tell the tale: molecular genetic methods for mycobacterial research. Int. J. Biochem. Cell Biol. 37:54-68.

  1. Mardassi H, Namouchi N, Haltiti R, Zarrouk M, Mhenni B, Karboul A, Khabouchi N, Gey van Pittius NC, Streicher EM, Rauzier J, Gicquel B, Dellagi K. 2005. Tuberculosis due to Resistant Haarlem Strain, Tunisia. Emerging Infectious Disease.11(6):957-961.

  2. Mostowy S, Inwald J, Gordon S, Martin C, Warren RM, Kremer K, Cousins D, Behr MA. 2005. Revisiting the Evolution of Mycobacterium bovis. Journal of Bacteriology.  187:6386-6395.

  1. Matsoso LG, Kana BD, Crellin PK, Lea-Smith D, Peolis A, Powell D, Dawes SS, Rubin H, Coppel RL, Mizrahi, V. 2005. Function of the cytochrome bc1-aa3 branch of the respiratory network in mycobacteria and network adaptation occurring in response to its disruption. J. Bacteriol. 187:6300-6307.

  2. Pheiffer C, Betts JC, Flynn HR, Lukey PT, van Helden PD. 2005. Protein expression by a Beijing strain differs from that of another clinical isolate and Mycobacterium tuberculosis H37Rv. Microbiology. 151:1139-1150.

  1. Schaaf HS, Krook S, Hollemans DW, Warren RM, Donald PR, Hesseling AC. 2005. Recurrent culture-confirmed tuberculosis in human immunodeficiency virus-infected children. Pediatr Infect Dis J. 24(8):685-91.

  2. Schaaf HS, Parkin DP, Seifart HI, Werely CJ, Hesseling PB, van Helden PD, Maritz JS, Donald PR. 2005. Isoniazid pharmacokinetics in children treated for respiratory tuberculosis. Arch. Dis. Child. 90:614-618.

  1. Shi L, Sohaskey CD, Kana BD, Dawes SS, North RJ, Mizrahi V, Gennaro ML. 2005. Changes in energy metabolism in Mycobacterium tuberculosis as revealed by transcriptional profiling during mouse lung infection and under in vitro conditions affecting aerobic respiration. Proc. Natl. Acad. Sci. USA. 102:15629-15634.

  2. Sholto-Douglas-Vernon C, Sandy J, Victor TC, Sim E, van Helden PD. 2005. Mutational and expression analysis of that and its response to isoniazid. Journal of Medical Microbiology. 54:1-9.

  1. Stopforth A, Tredoux A, Crouch A, van Helden P, Sandra P. 2005. A rapid method of diagnosing pulmonary tuberculosis using stir bar sorptive extraction-thermal desorption-gas chromatography-mass spectrometry. J Chromatogr A. 1071:135-9.

  2. van Rie A, Victor TC, Richardson M, Johnson R, van der Spuy GD, Murray EJ, Beyers N, Gey van Pittius NC, van Helden PD, Warren RM. 2005. Reinfection and mixed infection cause changing Mycobacterium tuberculosis drug-resistance patterns. Am J Respir Crit Care Med 1;172(5):636-42.

  1. Verver S, Warren RM, Beyers N, Richardson M, van der Spuy GD, Borgdorff MW, Enarson DA, Behr MA, van Helden PD. 2005. Rate of Reinfection Tuberculosis after Successful Treatment is Higher than Rate of New Tuberculosis. Am J Respir Crit Care Med. 171:1430-1435.

  2. Walzl G, Beyers N, van Helden P. 2005. TB: a partnership for the benefit of research and community. Trans R Soc Trop Med Hyg. 99 Suppl:515-519.

BOOKS

  • Chihota, VN, van Helden P, Victor TC, Warren RM. 2005 Molecular Epidemiology of Tuberculosis in South Africa:  Lessons learnt. In Molecular Epidemiology of tuberculosis, Eds. Academy of Science, Malaysia.

BOOK CHAPTERS

  • Mizrahi V, Buckstein M, Rubin H. 2005. Nucleic Acid Metabolism. In: Tuberculosis and the Tubercle Bacillus (Cole ST, Eisenach K, McMurray D, Jacobs WR Jr, eds), American Society for Microbiology Press, Washington DC, ch. 23, pp. 369-379.  ISBN: 1-55581-295-3.

  1. Brennan, MJ, Gey van Pittius NC, Espitia C. 2005. The PE and PPE multigene families of the Mycobacteria, In Tuberculosis and the Tubercle Bacillus (Cole ST, Eisenach K, McMurray D, Jacobs WR Jr, eds), American Society for Microbiology Press, Washington DC, ch. 33.  ISBN: 1-55581-295-3.

  2. Johnson R, Streicher EM, Louw GE, Warren RM, van Helden PD, Victor TC. 2005. Drug Resistance in Mycobacterium tuberculosis. In Mycobacterial Molecular Microbiology. Eds Horizon Scientific Press. (Chapter 5, Pages 169-197).

ABSTRACTS

  1. Fenhalls G, Stevens L, Sauk-Schubert C, Bezuidenhout J, Mustafa T, Litzky LA, Rubin H, Avarbock D, van Helden P, Lukey PT, Warren R, Walzl G.  Ziehl Neelsen and culture negative, yet metabolically active mycobacteria in sarcoid granulomas suggest that sarcoidosis is a persistent form of tuberculosis.  Blackwell Publishing Ltd, Immunology, 113, Supplement 1, 64.

CONFERENCES- INTERNATIONAL

  1. Bopape MC, Boshoff HI, Steel HC, Cockeran R, Matlola NM, Downing KJ, Mizrahi V, Anderson R. Phospholipases C and potassium transport knockout mutants of Mycobacterium tuberculosis to probe the molecular targets of antimicrobial action of clofazimine. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  1. Cynamon M, Sklaney M, Warren R, Victor T. Activities of isoniazid against isoniazid-resistant M. tuberculosis in a mouse model. The European Society of Mycobacteriology, Istanbul, Turkey. 2005.

  2. Dawes SS, Thomsen M, Freeman MF, Mizrahi V. The dual nature of Rv2228c, an Rnase H-CobC fusion? Poster presented at the Gordon Research Conference on vitamin B12 and corphins, Queens College, Oxford, UK, 8-13 September 2005.

  1. Gey van Pittius, NC, Botha, J, Madubanya, L, Van Helden, PD, and Warren, RM, Regulation of the Immunopathologically-important ESAT-6 Gene Cluster Operons. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  2. Gordhan BG, Smith DA, Bancroft GJ, Mizrahi V. Construction and phenotypic characterization of Mycobacterium tuberculosis mutants defective in genes induced in response to carbon starvation. Invited talk presented at the Conference on Functional Genomics for Novel Vaccine and Drug Design against Mycobacterial Infection, Department of Biotechnology, Indian Institute for Technology, Karagpur, India, 9-12 February 2005.

  1. Hayward D, Wiid I. Oxidative stress in Tuberculosis disease and treatment. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  2. Kana BD, Gordhan BG, Tsenova L, Sung N, Freeman S, Ellison E, Sacchettinin JC, Stoker NG, Mizrahi V. Characterization of a Y-family DNA polymerase from M. tuberculosis. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  1. Kana BD, Gordhan BG, Tsenova L, Sung N, Freeman S, Ellison E, Sacchettinin JC, Stoker NG, Mizrahi V. Characterization of a Y-family DNA polymerase from M. tuberculosis. Poster presented at the NITD Symposium on Tuberculosis, Bagamoyo, Tanzania, 16-20 October 2005

  2. Machowski EE, Warner DF, Barichievy S, Brackin R, Durbach SI, Kana BD, Springer B, Stoker NG and Mizrahi V. The development and application of reporter systems for assessing mutation rates in mycobacteria. Poster presented at the Joint Meeting of the International Union of Microbiological Sciences (IUMS 2005), San Fransisco, 23-28 July 2005. 

  1. Matsoso LG, Kana BD, Crellin PK, Lea-Smith DJ, Powell D, Dawes SS, Rubin H, Coppel RL, Mizrahi V. Function of the cytochrome bc1-aa3 branch of the respiratory network in mycobacteria and network adaptation occurring in response to its disruption. Poster presented at the Sixth International Conference on the Pathogenesis of Mycobacterial Infections, Stockholm, Sweden, 28-30 June 2005.

  2. Mizrahi V. Mutational mechanisms in mycobacteria. Invited lecture presented at the Joint Meeting of the International Union of Microbiological Sciences, IUMS 2005 Conference, San Fransisco, 23-28 July 2005.

  1. Mizrahi V. Post-Genomic Tuberculosis Research in South Africa - Lessons in Scientific Capacity Development. Invited lecture in honor of Ten years Post-Democracy, London School of Hygiene & Tropical Medicine, London, UK, 12 May 2005.

  2. Mizrahi, V. Participant at the Symposium on Science and Development to mark the 60th Anniversary of the CSIR, Pretoria, 28 September - 1 October, 2005.

  3. Mizrahi, V. The establishment and application of DNA microarray technology in the MMRU. Invited talk presented at the capar Microarray Technology Workshop, IIDMM, University of Cape Town, 25 October 2005.

  1. Mizrahi, V. Invited participant, Novartis Foundation Symposium on Innate Immunity in the Lung, Institute for Infectious Disease and Molecular Medicine, University of Cape Town, 27-30 November 2005.

  2. Mizrahi, V. Pathogenesis mechanisms in M. tuberculosis. Invited lecture presented at the EMBO/ Novartis Foundation/ ICGEB/ DST Workshop on Innate Immunity in the Lung, Institute for Infectious Disease and Molecular Medicine, University of Cape Town, 27-30 November 2005.

  1. Mizrahi, V. The application of molecular genetics in validating new TB drug targets. Plenary lecture presented at the IIDMM Grand Opening Conference: Understanding the Major Infectious Diseases of Africa, Cape Town, 22-24 March 2005.

  2. Mizrahi V. The role of error-prone DNA polymerases in damage tolerance and genetic adaptation in mycobacteria. Invited lecture, Dunn School of Pathology, Oxford University, Oxford, UK, 13 May 2005.

  1. Mizrahi, V. The challenges of applying new scientific tools to address the problem of TB in Africa. Plenary Address presented at the NITD Symposium on Tuberculosis, Bagamoyo, Tanzania, 16-20 October, 2005.

  2. Papavinasasundaram KG, Ko M, Chan BHY, Mizrahi V, Davis EO, Av-Gay Y. Roles of protein serine/ threonine kinases in M. tuberculosis physiology and virulence. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  1. Pheiffer C. Protein expression by a Beijing strain differs from that of another clinical  isolate and Mycobacterium tuberculosis H37Rv. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  2. Roberts T, Muller L and Walzl G. Phenotypic and functional characterization of immune suppressive T cells during Tuberculosis. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  1. Shi L, Kana BD, Sohaskey C, North RJ, Mizrahi V, Gennaro ML. The transitions to chronic infection in mouse lung and hypoxia-induced growth arrest in vitro are accompanied by a shift in the respiratory pathways of Mycobacterium tuberculosis. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  2. Van Helden PD. TB in the 21st Century. Changing TB Paradigms. Oslo, Norway 15-19 June 2005.

  1. Van Helden PD. Molecular Risk Factors for TB. Inflammatory Diseases of Barrier Organs. University Hospital Schleswig-Holstein, Germany. 3-4 June 2005.

  2. Van Helden PD. A search for surrogate markers for TB drug trials .NITD Symposium on Tuberculosis, Bagamoyo, Tanzania, October, 2005.

  1. Van Helden PD, Warren R, Michel A, Gey van Pittius NC, Hoal EG. Molecular characterization of animal and human mycobacterial isolates. Tropical animal Health and Production: Tuberculosis at the livestock / wildlife / human interface. Utrecht, Netherlands.October 2005.

  2. Van Helden PD. Forward to the Future, Tuberculosis Initiatives, GSk House, London, UK. 2005.

  1. Van Helden PD, Moller M, Babb C, Warren R, Walzl G, Uys P, Hoal E. TB Epidemiology and Human Genetics. Novartis Symposium: Innate Immunity to Pulmonary Infection, Cape Town 2005.

  2. Victor TC, Streicher EM, Warren RM, Jordaan AM, van der Spuy G, Kewley MC, Louw H, Bosman M, Murray M, van Helden PD. Clonal expansion of a highly drug resistant strain causes widespread MD-TB in South Africa. Poster presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

  1. Warner DF, Kana BD, Machoski EE, Brackin R, Gordhan BG, Dawes SS, Kaplan G, Stoker NG, Mizrahi V. The role of error-prone DNA polymerases in damage tolerance and genetic adaptation in mycobacteria. Lecture presented at the HHMI International Scholars Annual Meeting, Mérida, Mexico, 23-25 June, 2005

  2. Warner DF, Kana BD, Machowski EE, Brackin R, Gordhan BG, Dawes SS, Kaplan G, Barry CE III, Boshoff HIM, Stoker NG, Mizrahi V. The role of error-prone DNA polymerases in damage tolerance and genetic adaptation in mycobacteria. Invited lecture presented at the 2005 Keystone Symposium on Tuberculosis: Integrating Host and Pathogen Biology, Whistler, British Columbia, Canada, 2-7 April 2005.

CONFERENCES- NATIONAL

  1. Gey van Pittius, NC, Sampson, SL, van Helden, PD, and Warren, RM, Comparative Mycobacterial Genomics: uncovering the secrets of the genus Mycobacterium. Molecules-R-US, the 19th Meeting of the South African Society of Biochemistry and Molecular Biology, Stellenbosch, South Africa, January 2005.

  1. Mizrahi, V. Facilitator and co-organiser of the DST Workshop on National Priorities for Tuberculosis Research in South Africa, Cape Town, 9-10 November 2005.

  2. Mizrahi, V. Invited participant, Novartis Foundation Symposium on Innate Immunity in the Lung, Institute for Infectious Disease and Molecular Medicine, University of Cape Town, 27-30 November 2005.

  1. Mizrahi, V. Participant at the Symposium on Science and Development to mark the 60th Anniversary of the CSIR, Pretoria, 28 September - 1 October, 2005.

  2. Mizrahi, V. Pathogenesis mechanisms in M. tuberculosis. Invited lecture presented at the EMBO/ Novartis Foundation/ ICGEB/ DST Workshop on Innate Immunity in the Lung, Institute for Infectious Disease and Molecular Medicine, University of Cape Town, 27-30 November 2005.

  1. Mizrahi, V. The establishment and application of DNA microarray technology in the MMRU. Invited talk presented at the capar Microarray Technology Workshop, IIDMM, University of Cape Town, 25 October 2005.

  2. Pheiffer C, Betts JC, Flynn HR, Lukey PT, van Helden P. Differential protein expression by Mycobacterium tuberculosis strains. 19th South African Society of Biochemistry and Molecular Biology (SASBMB) conference. Stellenbosch, 2005.

  1. van Rie, A, Victor, T, Richardson, M, Johnson, R, van der Spuy, G, Murray, M, Beyers, N, Gey van Pittius, NC, van Helden, PD, Warren, RM, Mixed Mycobacterium tuberculosis infection: Changing drug-resistance patterns through antibiotic selection. (Poster). Molecules-R-US, the 19th Meeting of the South African Society of Biochemistry and Molecular Biology, Stellenbosch, South Africa, January 2005.

COMPLETED DOCTORAL THESES

  • Sholto-Douglas-Vernon, C. Characterization of the mycobacterial N-acetyltransferase. Stellenbosch Univ, 2005.

  1. Pheiffer, C.  Investigation of Mycobacterium tuberculosis protein expression and analysis of humoral immune responses of TB patients. Stellenbosch Univ, 2005.

  2. Warner, DF.  DNA metabolism in mycobacteria. Univ. Witwatersrand, 2005.

COMPLETED MASTERS DISSERTATIONS

  • Brackin, R. Expression and function of the mutator DNA polymerase-encoding umuC-like genes in mycobacteria. Graduated with Distinction, Univ. Witwatersrand, 2005.

  • Barichievy, S. Mutation rates in mycobacterial hosts with altered mutator DNA polymerase activity.  Graduated with Distinction, Univ. Witwatersrand, 2005.

2004

JOURNAL PUBLICATIONS

  • Boshoff, HIM., Barry, C.E. III and Mizrahi, V. 2004. Mutational dynamics in Mycobacterium tuberculosis: Implications for the evolution of drug resistance. S. Afr. J. Sci. 100:471-474.

  • Cowley, S, Ko, M, Pick, N, Chow, R, Downing, KJ, Gordhan, BG, Betts, JC, Mizrahi, V, Smith, DA, Stokes, RW, and Av-Gay, Y. 2004. The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamine/glutamate levels and is important for growth in vivo. Mol. Microbiol. 52:1691-1702.

  1. Downing, KJ, Betts, JC, Young, DI, Kelly, F, McAdam, R., Young, M, and Mizrahi, V. 2004. Global expression profiling of strains harbouring null mutations reveals that the five rpf-like genes of Mycobacterium tuberculosis show functional redundancy. Tuberculosis 84:167-179.

  2. Kana, BD, and Mizrahi, V. 2004. Molecular genetics of Mycobacterium tuberculosis in relation to the discovery of novel drugs and vaccines. Tuberculosis 84:63-75.

  1. Springer, B, Sander, P, Sedlacek, L, Hardt, WD, Mizrahi, V, Schär and Böttger, EC. 2004. Lack of mismatch correction facilitates genome evolution in mycobacteria. Mol. Microbiol. 53:1601-1609.

  2. Warner, DF and Mizrahi, V. 2004. Mycobacterial genetics in target validation. Drug Discovery Today: Technologies 1:93-98.

  1. Apers L, Chihota V. Susceptibility of a series of routine sputum samples for antituberculosis chemotherapy in Gweru, Zimbabwe. SAMJ 2004; 94:282.

  2. Boshoff, HIM., Barry, CE III and Mizrahi, V. 2004. Mutational dynamics in Mycobacterium tuberculosis: Implications for the evolution of drug resistance. S. Afr. J. Sci. 100:471-474.

  3. Cowley, S, Ko, M, Pick, N, Chow, R, Downing, KJ, Gordhan, BG, Betts, JC, Mizrahi, V, Smith, DA, Stokes, RW, and Av-Gay, Y. 2004. The Mycobacterium tuberculosis protein serine/threonine kinase PknG is linked to cellular glutamine/ glutamate levels and is important for growth in vivo. Mol. Microbiol. 52:1691-1702.

  1. Donald PR, Sirgel FA, Venter A, Parkin DP, Siefart HI, Wal BW, Werely C, van Helden PD, Maritz JS.  2004  The Influence of Human N-Acetyltransferase Genotype on the Early Bactericidal Activity of Isoniazid.  Clin Infect Dis  39 (10):1425-30

  2. Downing, KJ. Betts, JC, Young, DI, Kelly, F, McAdam, RA, Young, M, and Mizrahi, V. 2004. Global expression profiling of strains harbouring null mutations reveals that the five rpf-like genes of Mycobacterium tuberculosis show functional redundancy. Tuberculosis. 84:167-179.

  1. Eilers PH, Van Soolingen D, Thi Ngoc Lan N, Warren RM, Borgdorff MW. 2004. Transposition Rates of Mycobacterium tuberculosis IS6110 Restriction Fragment Length Polymorphism Patterns. J Clin Microbiol 42(6):2461-4.

  2. Fenhalls G. 2004. The use of Modern Molecular Techniques to Study Tuberculosis Immunology at the Site of the Disease. Current Allergy & Clinical Immunology. 17(4):167-172.

  1. Gey van Pittius NC, Sampson SL, Warren RM, van Helden PD.  Genome variation in Mycobacterium tuberculosis. 2004. South African Journal of Science 100 Pathogen Genomics, 465-470.

  2. Githui WA, Jordaan AM, Juma ES, Kinyanjui P, Karimi FG, Kimwomi J, Meme H, Mumbi P, Streicher EM, Warren R, van Helden PD, Victor TC. 2004. Identification of MDR-TB Beijing/W and other Mycobacterium tuberculosis genotypes in Nairobi, Kenya. Int J Tuberc Lung Dis. 8(3): 352-360.

  1. Hayward D, Wiid I, van Helden PD. 2004. Differential expression of mycothiol pathway genes: Are they affected by antituberculosis drugs? IUBMB Life. 56(3): 131-138.

  2. Hesseling AC, Schaaf HS, Victor T, Beyers N, Marais BJ, Cotton MF, Wiid I, Gie RP, van Helden P, Warren RM. 2004. Resistant M. bovis BCG disease: Implications for the management of BCG disease in HIV-infected children. Ped Infect Dis J. 23(5):476-9

  1. Hoal EG, Lewis LA, Jamieson S, Tanzer F, Rossouw M, Victor T, Hillerman R, Beyers N, Blackwell JM, van Helden PD. 2004. SLC11A1 (NRAMP1) but not SLC11A2 (NRAMP2) polymorphisms are associated with susceptibility to tuberculosis in a high incidence community in South Africa. Int J Tuberc. Lung Dis. 2004. 8(12):1464-71.

  2. Hoal EG, Möller M. 2004. Host Genetics and Predisposition to Tuberculosis. Current Allergy & Clinical Immunology. 17(4):160-165.

  1. Kana, B.D. and Mizrahi V. 2004 Molecular genetics of Mycobacterium tuberculosis in relation to the discovery of novel drugs and vaccines. Tuberculosis. 84:63-75.

  2. Loots DT, Wiid IJ, Page BJ, Mienie LJ, van Helden PD. (2004)  Melatonin prevents the free radical and MADD metabolic profiles induced by antituberculosis drugs in an animal model. Journal of Pineal Research. 38(2):100-106.

  1. Mokrousov I, Bhanu NV, Suffys PN, Kadival GV, Yap S-F, Cho S-N, Jordaan AM, Narvskaya O, Singh UB, Gomes HM, Lee H, Kulkarni SP, Lim K-C, Khan BK, van Soolingen D, Victor TC, van Embden JDA. 200. Multicenter evaluation of reverse line blot assay for detection of drug resistance in Mycobacterium tuberculosis clinical isolates. J Microbiological Methods. 57(3):323-35.

  2. Nguyen D, Brassard P, Menzies D, Thibert L, Warren R, Mostowy S, Behr M. 2004. Genomic Characterization of an Endemic Mycobacterium tuberculosis Strain: Evolutionary and Epidemiologic Implications. J Clin Microbiol. 42(6):2573-80.

  1. Richardson M, van der Spuy G, Sampson SL, Beyers N, van Helden PD, Warren RM. 2004. Stability of polymorphic GC-rich repeat sequence-containing regions of Mycobacterium tuberculosis. J Clin. Microbiol. 42 (3): 1302-1304.

  2. Sampson SL, Richardson M, van Helden PD, Warren RM. 2004 IS6110-Mediated deletion polymorphism in isogenic strains of Mycobacterium tuberculosis. J Clin. Microbiol. 42 (2): 895-898.

  1. Schaaf HS, Parkin DP, Seifart HI, Werely C, Hesseling PB, van Helden PD, Maritz JS. Isoniazid Pharmacokinetics in children treated for respiratory tuberculosis. Arch. Dis. Child. 90: 614 - 618.

  2. Streicher EM, Warren RM, Kewley C, Simpson J, Rastogi N, Sola C, van der Spuy G, van Helden PD, Victor TC. 2004 Genotypic and phenotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from rural districts of the Western Cape Province of South Africa. J Clin. Microbiol. 12 (2): 891-894.

  1. Verver S, Warren RM, Munch Z, Richardson M, van der Spuy GD, Borgdorff MW, Behr MA, Beyers N, van Helden PD. 2004 Proportion of tuberculosis transmission that takes place in households in a high incidence area. The Lancet 363 (9404): 212-214.

  2. Verver S, Warren RM, Munch Z, Vynnycky E, van Helden PD, Richardson M, van der Spuy GD, Enarson DA, Borgdorff MW, Behr MA, Beyers N. 2004. Transmission of tuberculosis in a high incidence urban community in South Africa. In J Epidemiol 33(2): 351-357.

  1. Victor TC, de Haas PEW, Jordaan AM, van der Spuy G, Richardson M, van Soolingen D, van Helden PD, Warren R. 2004. Molecular characteristics and global spread of Mycobacterium tuberculosis with a Western Cape F11 genotype. J. Clin. Microbiol. 42 (2): 769-772.

  2. Walzl G, Hussell T, Nel H. 2004. Is the Hygiene Effect bad news for the Control of Infectious Diseases? Current Allergy & Clinical Immunology. 17(4):180-186.

  1. Walzl G. 2004. Addressing the Tuberculosis and Infectious Diseases Burden. Current Allergy & Clinical Immunology. 17(4):158. (Editorial).

  2. Warner, D.F. and Mizrahi, V. 2004. Mycobacterial genetics in target validation. Drug Discovery Today: Technologies 1:93-98.

  1. Warren RM, Victor TC, Streicher EM, Richardson M, Beyers N, Gey van Pittius NC, van Helden PD. 2004 Patients with active tuberculosis often have different strains in the same sputum specimen. Am J Respir Crit Care Med. 169: 610-614.

  2. Warren RM, Victor TC, Streicher EM, Richardson M, van der Spuy GD, Johnson R, Chihota VN, Locht C, Supply P, van Helden PD. 2004.  Clonal Expansion of a Globally Dissemnated Lineage of Mycobacterium tuberculosis with Low IS6110 Copy Numbers. J Clin. Microbiol, 42(12):5774-5782.

  3. Wiid I, Seaman T, Hoal EG, Benade AJS, Paul D van Helden. 2004).Total antioxidant levels are low during active TB and rise with anti-tuberculosis therapy. Research Communication. IUBMB Life 56(2): 101-106.

BOOK CHAPTERS

  • Van Helden PD, Warren RM, Uys P, van der Spuy GD, Victor TC. The molecular epidemiology of MDR-TB, In: Management of Multiple Drug-Resistant Infections. Publisher - Humana Press Inc. Totowa, New Jersey. 2004 (Edited by Stephen H. Gillespie). Part III: Mycobacteria, Chapter 13: 225-242.

  1. Brennan, M.J., Gey van Pittius, N.C., Espitia, C., The PE and PPE multigene families of the Mycobacteria, In “Tuberculosis”, 2nd edition, Publisher - ASM Press, Washinton, USA, 2004 (Edited by Stewart T. Cole, Kathleen D. Eisenach, David N. McMurray and William R. Jacobs, Jr.). Part X: Host Immune Responses and Antigenic Variation, Chapter 33.  ISBN: 1-55581-295-3.

ABSTRACTS

  • Beyers N, Michaelis I, Gie R, Schaaf S, Richardson M, Warren R, Fourie B, van Helden P. 2004. Transmission of tuberculosis (TB) to children in a high incidence area.  Int. J. Tuberc. Lung Dis. 5(11):S185.

REGISTERED PATENTS

  • High Copy-number Plasmid Replicon. Patent holder: SA MRC/Univeristy of Stellenbosch. Inventors: Bourne, W, van Helden PD, Warren RM, Jansen Y. Feb 2004.

CONFERENCES - INTERNATIONAL

  • Bhakta S, Sholto-Douglas Vernon C, Samson S, Mo MSN, Upton A, van Helden P, Victor T, Sim E. Localisation and expression in Mycobacteria of Arylamine N-acetylatransferase, a novel target for anti-tuberclar drugs. TB Conference in New Delhi,  2004

  1. Mizrahi V. DNA metabolism in mycobacteria. Plenary lecture presented at the ICGEB Tuberculosis Symposium, International Center for Genetic Engineering and Biotechnology, New Delhi, India, 15-17th November 2004.

  2. Mizrahi V. Mutational dynamics in Mycobacterium tuberculosis: implications for the evolution of drug resistance and strain variation. Invited lecture presented at the Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, 19th May 2004.

  1. Mizrahi V. The development and application of genetic tools for investigating molecular mechanisms in Mycobacterium tuberculosis. Lecture presented at the 3rd Wellcome Trust/ EMBO Workshop: AIDS and TB, The Way Ahead. University of Cape Town, 29 November - 6 December 2004.

  2. Ramoutar, R “The Induction of Cellular Immune Responses by Mycobacterial Antigens in a Family-based Study in South Africa” to be presented July in Montreal at the FOCIS/ICI 2004 Immunology Congress (Poster).

  1. Streicher EM, Warren RM, Jordaan AM, Johnson R, Engelke E, Kewley C, Bosman M, van der Spuy G, van Helden PD, Victor TC. Charisteristics of Micro-epidemics of Drug Resistant Mycobacterium Tuberculosis Isolates from Rural Districts of the Western Cape Province in South Africa. American Society for Microbiology, New Orleans, Louisiana, May 2004

  2. Victor T, Streicher L, Jordaan A, van Helden P, Johnson R, Warren R. Drug resistant tuberculosis in the Western Cape. Imperial College, London. May 2004.

  1. Walzl, G. American Society for Tropical Medicine and Hygiene Society Conference in Miami 7-11 November 2004.

  2. Walzl, G.  British Society of Immunology Annual Congress, Harrogate, UK, 7-10 Dec 2004.

  1. Warner DF, Machowski EE, Kana BD, Barichievy S, Brackin R, Stoker NG, Sacchettini JC, Boshoff HIM, Dawes SS, Mizrahi V. The individual and collective roles of mutator DNA polymerases in genetic adaptation and long-term survival of mycobacteria. Poster presented at the Annual International Scholars meeting of the Howard Hughes Medical Institute, Tallin, Estonia, 23-28th June 2004.

  2. Warner DF, Machowski EE, Kana BD, Barichievy S, Brackin R, Stoker NG, Boshoff HIM, Dawes SS, Mizrahi V. Y-family polymerases in mycobacteria: roles in genetic adaptation and long-term survival. Poster presented at the ASM Conference on DNA Repair and Mutagenesis: From Molecular Structure to Biological Consequences, Southampton,
CONFERENCES NATIONAL (SOUTH AFRICAN)
  1. Hoal van Helden EG.   Is Tuberculosis in the Genes? 18th Congress of the South African Genetics Society, Stellenbosch. 4 - 7 April 2004, Stellenbosch. Western Cape province.

  1. Chihota V, Apers L, Mungofa S, Kasongo W, Nyoni I, Ndabambi S, Streicher EM, Victor T, van Helden P, Warren R.  Predominance of the SAF1 Genotype of M. Tuberculosis in Regions of Southern Africa. 48th Academic Yearday of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2004.

  2. Dabbs, E.R. and Mowa MB. Cloning and characterization of a gene responsible for acrylamide bioremediation. MCBG Annual Symposium, University of the Witwatersrand, Johannesburg, 6th October 2004.

  1. Streicher EM, Warren RM, Jordaan AM, Johnson R, Engelke E, Kewley C, Simpson J, van der Spuy G, Sola C, Rastogi N, van Helden PD, Victor TC. Micro-epidemics of drug resistant Mycobacterium tuberculosis. 18th Congress of the South African Genetics Society, 4 - 7 April 2004, Stellenbosch. Western Cape province.

  2. Gey van Pittius NC, Sampson SL, Lee H, Kim Y, van Helden, PD, and Warren RM  The evolution of the Mycobacterium tuberculosis PE and PPE multigene families. 48th Academic Yearday of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2004.

  1. Gordhan BG, Smith DA, Mizrahi V. Construction and phenotypic characterization of Mycobacterium tuberculosis mutants defective in genes induced in response to carbon starvation. Poster presented at the Research Day, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 4th August 2004.

  2. Johnson R, Warren R, van Helden P, Streicher E, Victor T.  Molecular Characterization of the Drug Resistant Beijing / W-like Strain. 48th Academic Yearday of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2004.

  1. M Rossouw, H Nel, C Babb, P van Helden, E Hoal.  The Importance of Interferon-Gamma. 18th Congress of the South African Genetics Society, 4 - 7 April 2004, Stellenbosch. Western Cape province: Micro-epidemics of drug resistant Mycobacterium tuberculosis.

  2. Mizrahi V. Building a Center of Excellence in Biomedical Research in South Africa - Opportunities and Challenges. Special Address presented at the Research Day, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 4th August 2004.

  1. Mizrahi V. Taming the White Plague: Post-genomic tools for identifying and validating targets for new TB drugs and vaccines. Inaugural Distinguished Science Alumni Lecture, University of Cape Town, 27th October 2004.

  2. Johnson R, Jordaan AM, Pretorius L, Engelke E, Kewley C, Bosman M, van Helden PD, Warren R, and Victor TC.  Molecular Phenotyping: An Adjunct to Management of Drug Resistant - tuberculosis. 18th Congress of the South African Genetics Society, 4 - 7 April 2004, Stellenbosch. 

  1. Streicher EM, Warren RM, Jordaan AM, van der Spuy GD, Kewley Bosman M, Sola C, Rastogi N, van Helden PD, Victor TC.  Clonal Expansion of a highly drug resistant strain causes widespread MDR-TB in South Africa.  48th Academic Yearday of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2004.

  2. Roos T, van Helden P, Hoal E.  Association Between Surfactant Protein D Polymorphisms and Serum Concentration. (Poster).18th Congress of the South African Genetics Society, Stellenbosch, 4-7 April 2004. 

  3. Victor T.  Control of drug resistant Tuberculosis: a molecular approach.2004 4th Academic Day, SMLTSA, Tygerberg Branch, May 2004.

  1. Victor T. Micro-epidemics of drug resistant TB in local communities.National Tuberculosis control program task force meeting: February 2004.

  2. Van Helden PD. Genotype and Phenotype: From Bacteria to Humans. 18th Congress of the South African Genetics Society, Stellenbosch, 4-7 April 2004. 

  1. Van Rie A, Victor T, Richardson M, Johanson R, van der Spuy G, Murray E, Beyers N, van Helden P, Warren R.  Alternating Drug Resistance patterns during Tuberculosis treatment:  Acquisition, Reinfection or Multiple infection?   48th Academic Yearday of the Faculty of Health Sciences of the University of Stellenbosch, Tygerberg, August 2004.

  2. Warren R, van Rie A, Victor T, Gey van Pittius N, Richardson M, Streicher E, Johnson R, van der Spuy G, Beyers N, van Helden P.  Population structure of Mycobacterium Tuberculosis in the human host. Path Splash, FSASP 44th Annual Conference with SASCC, 4 - 7 July 2004, Stellenbosch. 

COMPLETED DOCTORAL THESES

  1. Adams JFA. Immune responses in a community with a high incidence of tuberculosis. Stellenbosch Univ. March 2004.

COMPLETED MASTERS DISSERTATIONS

  • Barnard, D. Risk Factors associated with the development of INH resistance in Beijing/W isolates.  Nucleotide sequence variation and expression levels of critical genes associated with cancer of the upper gastro-intestinal tract. Stellenbosch Univ. 2004.

  • Lundwall-Roos TA.  An Investigation into the Role of Collectins in Tuberculosis Infection. Stellenbosch Univ, 2004.

 


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