Research Outputs for 2010

Books / Chapters in Books (Total: 2)

  • McEvoy CRE, Warren RM, van Helden PD. 2010. Molecular Methods and Their Application in TB Epidemiology. In: Schaaf HS, Zumla AI (ed.) Tuberculosis. A Comprehensive Clinical Reference. Elsevier Publications. pp 28 – 37.
  • Dheda K, Warren RM, Zumla A, Grobusch MP. 2010. Extensively drug-resistant tuberculosis: epidemiology and management challenges. Infect Dis Clin North Am 24:705-725.

Articles in Peer-Reviewed Journals (Total: 46)

  • Warner DF, Ndwandwe DE, Abrahams GL, Kana BD, Machowski EE, Venclovas C, Mizrahi V. (2010) Essential roles for imuA'- and imuB-encoded accessory factors in DnaE2-dependent mutagenesis in Mycobacterium tuberculosis. Proc Natl Acad Sci USA. 107(29):13093-13098. (IF=10.312)
  • Kana BD, Mizrahi V, Gordhan BG. (2010) Depletion of resuscitation-promoting factors has limited impact on the drug susceptibility of Mycobacterium tuberculosis. J Antimicrob Agents Chemother. 65(8):1583-1585 (IF=4.294)
  • Ioerger TR, Feng Y, Ganesula K, Chen X, Dobos KM, Fortune S, Jacobs WR Jr, Mizrahi V, Parish T, Rubin E, Sassetti C, Sacchettini JC. (2010) Variation among genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories. J Bacteriol. 192(14):3645-3653. (IF=4.035)
  • Kana BD, Abrahams GL, Sung N, Warner DF, Gordhan BG, Machowski EE, Tsenova L, Sacchettini JC, Stoker NG, Kaplan G, Mizrahi V. (2010) Role of the DinB homologs Rv1537 and Rv3056 in Mycobacterium tuberculosis. J Bacteriol. 192(8):2220-2227. (IF=4.035)
  • Capron M, Mizrahi V. (2010) Highlights from the first meeting of the Europe-Africa frontier research conference series infectious diseases: from basic to translational research. FEMS Immunol Med Microbiol. 58(1):1-2. (IF=2.352)
  • Kana BD, Mizrahi V. (2010) Resuscitation-promoting factors as lytic enzymes for bacterial growth and signalling. FEMS Immunol Med Microbiol. 58(1):39-50. (IF=2.352)
  • Aguilar D, Hanekom M, Mata D, Gey van Pittius NC, van Helden PD, Warren RM, Hernandez-Pando R. (2010) Mycobacterium tuberculosis strains with the Beijing genotype demonstrate variability in virulence associated with transmission. Tuberculosis (Edinb) 90:319-25. (IF=2.986)
  • Alexander KA, Laver PN, Michel AL, Williams M, van Helden PD, Warren RM, Gey van Pittius NC. (2010) Novel Mycobacterium tuberculosis complex pathogen, M. mungi. Emerg Infect Dis 16:1296-9. (IF=6.497)
  • Calver AD, Falmer AA, Murray M, Strauss OJ, Streicher EM, Hanekom M, Liversage T, Masibi M, van Helden PD, Warren RM, Victor TC. (2010) Emergence of increased resistance and extensively drug-resistant tuberculosis despite treatment adherence, South Africa. Emerg Infect Dis 16:264-271. (IF=6.497)
  • Cilliers K, Labadarios D, Schaaf HS, Willemse M, Maritz JS, Werely CJ, Hussey G, Donald PR. (2010) Pyridoxal-5-phosphate plasma concentrations in children receiving tuberculosis chemotherapy including isoniazid. Acta Paediatr. 99(5):705-710 (IF=1.995)
  • Cobat A, Gallant CJ, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Hoal EG, Alcaïs A, Schurr E. (2010) High heritability of antimycobacterial immunity in an area of hyperendemicity for tuberculosis disease. J Infect Dis 201:15-9. (IF=5.698)
  • De Souza GA, Fortuin S, Aguilar D, Hernandez Pando R, McEvoy CRE, van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG (2010). Using a label-free proteomic method to identify differentially abundant proteins in closely related hypo- and hyper-virulent clinical Mycobacterium tuberculosis Beijing isolates. Mol. Cell Proteomics. 9: 2414-2423. (IF=9.606)
  • Demers A-M, Boulle A, Warren RM, Verver S, van Helden PD, Behr MA, Coetzee D. (2010) Use of simulated sputum specimens to estimate the specificity of laboratory-diagnosed tuberculosis. Int J Tuberc Lung Dis 14:1016-23. (IF=2.442)
  • Demers A-M, Mostowy S, Coetzee D, Warren RM, van Helden PD, Behr MA. (2010) Mycobacterium africanum is not a major cause of human tuberculosis in Cape Town, South Africa. Tuberculosis (Edinb) 90:143-4. (IF=2.986)
  • Dheda K, Shean K, Zumla A, Badri M, Streicher EM, Page-Shipp L, Willcox P, John M-A, Reubenson G, Govindasamy D, Wong M, Padanilam X, Dziwiecki A, van Helden PD, Siwendu S, Jarand J, Menezes CN, Burns A, Victor TC, Warren RM, Grobusch MP, van der Walt M, Kvasnovsky C. (2010) Early treatment outcomes and HIV status of patients with extensively drug-resistant tuberculosis in South Africa: a retrospective cohort study. Lancet 375:1798-807. (IF=29.443)
  • Dheda K, Warren RM, Zumla A, Grobusch MP. 2010. Extensively drug-resistant tuberculosis: epidemiology and management challenges. Infect Dis Clin North Am 24:705-725. (IF=2.290)
  • Diacon AH, Dawson R, Hanekom M, Narunsky K, Maritz SJ, Venter A, Donald PR, Van Niekerk C, Whitney K, Rouse DJ, Laurenzi MW, Ginsberg AM, Spigelman MK. (2010) Early bactericidal activity and pharmacokinetics of PA-824 in smear-positive Tuberculosis patients. Antimicrob Agents Chemother. 54(8): 3402-3407. (IF=4.294)
  • Diacon AH, Maritz JS, Venter A, Van Helden PD, Andries K, McNeeley DF, Donald PR. (2010) Time to detection of the growth of Mycobacterium tuberculosis in MGIT 960 for determining the early bactericidal activity of antituberculosis agents. Eur J Clin Microbiol Infect Dis. 29 (12): 1561-5. (IF=2.560)
  • Gallant CJ, Cobat A, Hoal EG, Schurr E. (2010) Quantifying latent TB infection: The dilemma continues. Chest. 138: 460-461. (IF=5.442)
  • Gallant CJ, Cobat A, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Alcaïs A, Hoal EG, Schurr E. (2010) Impact of age and sex on mycobacterial immunity in an area of high tuberculosis incidence. Int J Tuberc Lung Dis 14:952-9. (IF=2.442)
  • Gallant CJ, Cobat A, Simkin L, Black GF, Stanley K, Hughes J, Doherty TM, Hanekom WA, Eley B, Beyers N, Jaïs J-P, van Helden PD, Abel L, Alcaïs A, Hoal EG, Schurr E. (2010) Tuberculin skin test and in vitro assays provide complementary measures of antimycobacterial immunity in children and adolescents. Chest 137:1071-7. (IF=5.442)
  • Govender L, Abel B, Hughes EJ, Scriba TJ, Kagina BM, de Kock M, Walzl G, Black G, Rosenkrands I, Hussey GD, Mahomed H, Andersen P, Hanekom WA. (2010) Higher human CD4 T cell response to novel Mycobacterium tuberculosis latency associated antigens Rv2660 and Rv2659 in latent infection compared with tuberculosis disease. Vaccine 29(1): 51-57. (IF=3.508)
  • Harper CJ, Hayward D, Kidd M, Wiid I, van Helden PD. (2010) Glutamate dehydrogenase and glutamine synthetase are regulated in response to nitrogen availability in Myocbacterium smegmatis. BMC Microbiol 10:138. (IF=3.329)
  • Hesseling AC, Marais BJ, Kirchner HL, Mandalakas AM, Brittle W, Victor TC, Warren RM, Schaaf HS. (2010) Mycobacterial genotype is associated with disease phenotype in children. Int J Tuberc Lung Dis 14:1252-8. (IF=2.442)
  • Hesseling AC, Walzl G, Enarson DA, Carroll NM, Duncan K, Lukey PT, Lombard C, Donald PR, Lawrence KA, Gie RP, van Helden PD, Beyers N. (2010) Baseline sputum time to detection predicts month two culture conversion and relapse in non-HIV-infected patients. Int J Tuberc Lung Dis 14:560-70. (IF=2.442)
  • Human H, Hagen CM, de Jong G, Harris T, Lombard D, Christiansen M, Bardien S. (2010) Investigation of mitochondrial sequence variants associated with aminoglycoside-induced ototoxicity in South African TB patients on aminoglycosides. Biochem Biophys Res Commun 393:751-6. (IF=2.270)
  • Innes S, Schaaf HS, Hoek KGP, Rabie H, Cotton MF. (2010) Unsuspected fatal drug-resistant tuberculosis in a closely monitored child: a plea for improved source-case tracing and drug susceptibility testing. South Afr J Epidemiol Infect. 25(2): 30-32. (IF not available)
  • Ioerger TR, Feng Y, Chen X, Dobos KM, Victor TC, Streicher EM, Warren RM, Gey van Pittius NC, Van Helden PD, Sacchettini JC. (2010) The Non-clonality of Drug Resistance in Beijing-genotype Isolates of Mycobacterium tuberculosis from the Western Cape of South Africa. BMC Genomics. 11: 670. (IF=4.179)
  • Johnson R, Warren RM, van der Spuy GD, Gey van Pittius NC, Theron D, Streicher EM, Bosman M, Coetzee GJ, van Helden PD, Victor TC. (2010) Drug-resistant tuberculosis epidemic in the Western Cape driven by a virulent Beijing genotype strain. Int J Tuberc Lung Dis 14:119-21. (IF=2.442)
  • Koegelenberg CFN, Bolliger CT, Theron J, Walzl G, Wright CA, Louw M, Diacon AH. (2010) Direct comparison of the diagnostic yield of ultrasound-assisted Abrams and Tru-Cut needle biopsies for pleural tuberculosis. Thorax 65:857-62. (IF=6.906)
  • Mallard K, McNerney R, Crampin AC, Houben R, Ndlovu R, Munthali L, Warren RM, French N, Glynn JR. (2010) Molecular detection of mixed infections of Mycobacterium tuberculosis in sputum samples from patients in Karonga District, Malawi. J Clin Microbiol. 48 (12): 4512-4518. (IF=4.126)
  • Michel AL, Müller B, van Helden PD. (2010) Mycobacterium bovis at the animal-human interface: a problem, or not? Vet Microbiol 140:371-381.
  • Möller M, de Wit E, Hoal EG. (2010) Past, present and future directions in human genetic susceptibility to tuberculosis. FEMS Immunol Med Microbiol 58:3-26. (IF=2.352)
  • Möller M, Flachsbart F, Till A, Thye T, Horstmann RD, Meyer CG, Osei I, van Helden PD, Hoal EG, Schreiber S, Nebel A, Franke A. (2010) A functional haplotype in the 3'untranslated region of TNFRSF1B is associated with tuberculosis in two African populations. Am J Respir Crit Care Med 181:388-93. (IF=9.881)
  • Möller M, Hoal EG. (2010) Current findings, challenges and novel approaches in human genetic susceptibility to tuberculosis. Tuberculosis (Edinb) 90:71-83. (IF=2.986)
  • Möller M, Nebel A, van Helden PD, Schreiber S, Hoal EG. (2010) Analysis of eight genes modulating interferon gamma and human genetic susceptibility to tuberculosis: a case-control association study. BMC Infect Dis 10:154. (IF=2.715)
  • Muyoyeta M, de Haas PEW, Mueller DH, van Helden PD, Mwenge L, Schaap A, Kruger C, Gey van Pittius NC, Lawrence K, Beyers N, Godfrey-Faussett P, Ayles H. (2010) Evaluation of the Capilia TB assay for culture confirmation of Mycobacterium tuberculosis infections in Zambia and South Africa. J Clin Microbiol 48:3773-5. (IF=4.126)
  • Onajole OK, Govender P, van Helden PD, Kruger HG, Maguire GEM, Wiid I,  Govender T. (2010) Synthesis and evaluation of SQ109 analogues as potential anti-tuberculosis candidates. Eur J Med Chem 45:2075-9. (IF=3.363)
  • Parsons SDC, de Villiers C, Gey van Pittius NC, Warren RM, van Helden PD. (2010) Detection of Mycobacterium kansasii infection in a rhesus macaque (Macaca mulatta) using a modified QuantiFERON-TB Gold assay. Vet Immunol Immunopathol 136:330-4. (IF=2.211)
  • Repsilber D, Kern S, Telaar A, Walzl G, Black GF, Selbig J, Parida SK, Kaufmann SHE, Jacobsen M. (2010) Biomarker discovery in heterogeneous tissue samples -taking the in-silico deconfounding approach. BMC Bioinformatics 11:27. (IF=4.108)
  • Roberts L, Passmore JS, Williamson C, Little F, Bebell LM, Mlisanac K, Burgers WA, van Loggerenberg F, Walzl G, Djoba Siawaya JF, Abdool Karim Q and Abdool Karim SS. (2010) Plasma cytokine levels during acute HIV-1 infection predict HIV disease progression. AIDS  24(6):819–831. (IF=5.334)
  • Sester M, Giehl C, McNerney R, Kampmann B, Walzl G, Cuchí P, Wingfield C, Lange C, Migliori GB, Kritski AL, Meyerhans A; European Network for global cooperation in the field of AIDS and TB (EUCO-Net). (2010) Challenges and perspectives for improved management of HIV/Mycobacterium tuberculosis co-infection. Eur Respir J. 36(6):1242-1247.  (IF=5.076)
  • Stefan DC, Dippenaar A, Detjen AK, Schaaf HS, Marais BJ, Kriel B, Loebenberg L, Walzl G, Hesseling AC. (2010) Interferon-gamma release assays for the detection of Mycobacterium tuberculosis infection in children with cancer. Int J Tuberc Lung Dis. 14(6): 689-694. (IF=2.442)
  • Wallis RS, Pai M, Menzies D, Doherty TM, Walzl G, Perkins MD, Zumla A. (2010) Biomarkers and diagnostics for tuberculosis: progress, needs, and translation into practice. Lancet 375:1920-37. (IF=29.443)
    Wright CA, Bamford C, Prince Y, Vermaak A, Hoek KGP, Marais BJ, Warren RM. (2010) Mycobacterial transport medium for routine culture of fine needle aspiration biopsies. Arch Dis Child 95:48-50. (IF=2.620)
  • Wright CA, Hoek KGP, Marais BJ, van Helden PD, Warren RM. (2010) Combining fine-needle aspiration biopsy (FNAB) and high-resolution melt analysis to reduce diagnostic delay in Mycobacterial lymphadenitis. Diagn Cytopathol 38:482-8. (IF=1.215)

Non Peer-Reviewed Articles (Total: 3)

  • Marais BJ, van Helden PD, Donald PR. (2010) Adequacy of 6-month follow-up in chemotherapy trials. Int J Tuberc Lung Dis 14:927; author reply 928. (IF=2.442)
  • Van Helden PD. (2010) Is bigger better? EMBO Reports 11:408.
  • Van Helden PD. (2010) Managing Academic Research. EMBO Reports 11:648.

Published Abstracts (Total: 0)

Technical Reports (Total: 0)

Products / Artefacts / Patents (Total: 0)

Conferences/Meetings Attended & Invited Talks/Seminars Presented (Total: 89)

  • Abrahams GL, Wei H, Ciulli A, Sherman DR, Abell C, Barry CE III, Boshoff HIM,  Mizrahi V. New tools for tuberculosis drug discovery: Development of novel screening strains of M. tuberculosis and application in target-based approaches. Plenary lecture presented at the Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, NM, 14-19 February 2010.
  • Wei AH, Silvestre HL, Wen S, Abrahams GL, Sherman DR, Barry CE III, Boshoff HIM, Ciulli A, Blundell TL, Abell C. Application of fragment-based approaches to the discovery of new agents against Mycobacterium tuberculosis. Plenary lecture presented at the  Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, New Mexico, 13-19 February, 2010
  • Mizrahi V. Early-stage drug discovery. Plenary lecture presented at the NIH/HHMI/SA MRC US-Southern Africa Joint Research Forum on Tuberculosis, Pretoria, 1-2 March 2010.
  • Mizrahi V. Summary of the NIH/HHMI/SA MRC US-Southern Africa Joint Research Forum on Tuberculosis. Talk presented at the Research Forum (2 March 2010), and at the following Institute of Medicine- Academy of Science of South Africa Workshop on the Emerging threat of drug-resistant tuberculosis: Global and local challenges and solutions, Pretoria, 3-4 March 2010.
  • Abrahams GL, Mizrahi V. Target-based whole-cell screening. Talk presented at the IMTB Annual Meeting, Martha’s Vineyard, MA, 5-6 May 2010.
  • Mizrahi V. Taking advantage of stress: DNA damage, mutagenesis and the evolution of drug resistance in M. tuberculosis. Division U Plenary Lecture, presented at the 110th General Meeting of the American Society for Microbiology, San Diego, CA. 24-28 May 2010.
  • Cholo MC, Cockeran, R., Mizrahi, V., van Rensburg, E.J., Stoker, N.G., and Anderson, R. Evaluation of the Relative Efficiencies and Preferential Utilization at Various Stages During Growth of the Trk and Kdp Potassium Transporters of Mycobacterium Tuberculosis. Talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Koch A, Mizrahi V, Warner DF. The fitness cost of drug resistance mutations in mycobacteria. Invited talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Gordhan BG. The role of DNA glycosylases in mutagenesis and adaptation to stress in mycobacteria. Invited talk presented at the 2nd SA TB Conference, Durban, 2-5 June 2010.
  • Kana BD.  From TB Pathogenesis to Drug Development. Plenary lecture presented at the 2nd SA TB Conference, Durban, 2-5 June 2010
  • Warner DF.  A novel mechanism of damage-induced mutagenesis in Mycobacterium tuberculosis. Invited talk presented to the National TB Reference Laboratory, National Institute for Communicable Diseases, Johannesburg, 19 July 2010.
  • Warner DF. Taking advantage of stress: DNA damage-induced mutagenesis in Mycobacterium tuberculosis.  Invited talk presented to a visiting delegation led by Prof. Ronald Atlas under the People To People Citizen Ambassador Programme, University of the Witwatersrand, 21 July 2010.
  • Mizrahi V. TB Drug Discovery Challenges from a Mycobacterial Physiology Perspective. Invited talk presented at the Scientific Advisory Committee meeting of the Global Alliance for TB Drug Development, New York, 28 July 2010.
  • Mizrahi V. Taking Advantage of Stress: DNA damage, mutagenesis and the evolution of drug resistance in M. tuberculosis. Plenary Lecture delivered at the UKZN College of Health Sciences Research Symposium, Durban, 15 September 2010.
  • Ahidjo BA, Gordhan BG, Mizrahi V. Nuclease-like VapC toxins from mycobacterium tuberculosis differentially inhibit mycobacterial growth and are specifically neutralised by their cognate antitoxins. Invited talk presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010. [Winner, Best Young Researcher Award, Infectious Diseases category]
  • Koch A, Mizrahi V, Warner DF. The fitness cost of rifampicin resistance in mycobacteria. Invited talk presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Koch A, Mizrahi V, Warner DF. The physiological implications of drug resistance in Mycobacteria. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Ndwandwe DE, Mizrahi V, Warner DF. A novel mutasome in Mycobacterium tuberculosis. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Moosa A, Mizrahi V, Warner DF. The metabolism of vitamin B12 in Mycobacterium tuberculosis: role of alternate B12 cofactors. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Naran K, Veale R, Mizrahi V, Warner DF. Characterisation of the anti-mycobacterial effect of a Pseudomonas-derived activity. Talk presented at the 3rd Postgraduate Cross-Faculty Symposium, Wits University, 27 October 2010.
  • Abrahams GL, Wei H, Ciulli A, Sherman DR. Abell C, Barry CE III, Boshoff HIM,  Mizrahi V. New tools for tuberculosis drug discovery: Development of novel screening strains of M. tuberculosis and application in target-based approaches. Poster lecture presented at the Keystone Symposium on Antibiotics and Resistance: Challenges and Solutions, Santa Fe, NM, 14-19 February 2010.
  • Kana BD, Mizrahi V, Gordhan, BG. The role of resuscitation promoting factors in drug susceptibility and stress adaptation of Mycobacterium tuberculosis. Poster presented at the Gordon Research Conference on Bacterial Cell Surfaces, Colby Sawyer College, NH, USA, 27 June – 2 July 2010.
  • Koch A, Mizrahi V, Warner DF. The fitness cost of rifampicin resistance in mycobacteria. Poster presented at the FEBS-EMBO Lecture Course on Host-Microbe Interactions, Spetses, Greece, 3-10 September 2010. [Winner, Best Poster Prize]
  • Naran K, Mizrahi V, Warner DF. A Pseudomonas-derived extract that inhibits growth of Mycobacterium tuberculosis Poster presented at the FEBS-EMBO Lecture Course on Host-Microbe Interactions, Spetses, Greece, 3-10 September 2010.
  • Moosa A, Mizrahi V, Warner DF. A role for a mycobacterium-specific protein family in vitamin B12 biosynthesis in Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010. [Winner, Best Poster Prize, Infectious Diseases category]
  • Naran K, Mizrahi V, Warner DF. A Pseudomonas-derived extract that inhibits growth of Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Narandes N, Mizrahi V, Kana BD. Functional characterisation of molybdopterin synthase-encoding genes in mycobacteria. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Ndwandwe DE, Mizrahi V, Warner DF. A novel mutasome in Mycobacterium tuberculosis. Poster presented at the Faculty of Health Sciences Research Day and Postgraduate Expo, University of the Witwatersrand, 22 September 2010.
  • Ahmadou Ahidjo B, Kuhnert D, Machowski EE, Gordhan BG, Abrahams GL, Mizrahi V. VapC toxins from Mycobacterium tuberculosis differentially inhibit mycobacterial growth and are specifically neutralised by their cognate antitoxins. Poster presented at the NITD Symposium on Tuberculosis 2010, Yaounde, Cameroon, 11-15 October 2010.
  • Mapela L, Kana BD. Characterisation of resuscitation promoting factors in M. smegmatis. Poster presented at the MRC Research Day, Cape Town, 14 October 2010.
  • Narrandes N, Mizrahi V, Kana BD. Functional characterization of molybdopterin synthase-encoding genes in mycobacteria. Poster presented at the MRC Research Day, Cape Town, 14 October 2010.
  • Lucas LA, Möller M, Nebel A, Van Helden PD, Schreiber S, Hoal EG. The role of Toll like receptors in genetic host susceptibility to tuberculosis. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Muleya V. Structural Characterisation of the Interaction Between RBBP6 and the Multifunctional Protein YB-1.  Talk presented at the 10th Astrazeneca Health Sciences Research Day. University of Stellenbosch, 25 February 2010.
  • Ngombane N, Ottenhoff THM, Kidd M, Walzl G, Black G. The predictive role of cytokine expression patterns of recently exposed tuberculin skin test negative TB contacts in the Western Cape Province. Talk presented at the 10th AstraZeneca Health Sciences Research Day, University of Stellenbosch, South Africa, 25 February 2010.
  • Salie M, Möller M, Van Helden PD, Schreiber S, Hoal EG, Nebel A, Hofmann S. A 3’UTR polymorphism in ANXA11 is associated with pulmonary tuberculosis in the South African coloured population. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Wagman C, Möller M, Hoal EG. Marco haplotypes and susceptibility to tuberculosis. Talk presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Warren RM. Molecular and Genetic epidemiology of TB in South Africa. Invited talk presented at the US- Southern Africa Joint research forum on Tuberculosis. Roode Valley Country Lodge, Pretoria, South Africa, 1-2 March 2010.
  • Warren RM; Genetic analysis of drug resistant strains in South Africa. Invited stalk at the ASSAF meeting on MDR-TB, Pretoria, 3-4 March 2010.
  • De Vos M. Rifampicin induces differential protein expression in Mycobacterium tuberculosis. Invited talk at the 2nd TB Conference on forging strategic partnerships to Fight TB and HIV. Durban, South Africa, 2-3 June 2010.
  • Van Helden PD. Quantitative first- and second-line drug susceptibility testing (DST) on MGIT 960 using BD EpiCenter Software. Invited talk presented at the 2nd TB Conference on Forging strategic partnerships to Fight TB and HIV. Durban, South Africa, 2-3 June 2010.
  • Black PA, Warren RM, Louw GE, Van Helden PD, Victor TC. Identification of novel mechanisms of drug resistance in Mycobacteria byTransposon Mutagenesis. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • De Vos M, Warren RM, Johnson R, Van Helden PD, Ndimba BK, Victor RC. Rifampicin induces differential protein expression in Mycobacterium Tuberculosis. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Hoal EG.  The History in our Genes: the Complex Structure of the South African Coloured Population.  Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Macingwana L, Baker B, Ngwane A, Van Helden PD, Cotton M, Wiid IJF. Investigation of synergistic effect of Isoniazid, Sulfamethoxazole and Trimethoprim as potential first-line combination drug regimen against Mtb. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Streicher EM, Gey Van Pittius NC, De Kock M, Van Helden PD, Victor TC, Warren RM. Acquisition of resistance to anti-Tuberculosis drugs may compromise the utility of genetic based drug resistance tests. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Tait M, Sirgel F, Trollip A, Warren RM, Coetzee G, Victor TC. Cross-resistance compromises the use of Capreomycin in the treatment of XDR-TB. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Hoek KGP, Wright CA, Marais BJ, Van Helden PD, Warren RM. High-resolution melt analysis for the detection of Mycobacterial Lymphadenitis using Whatman Fta® Elute cards. Talk presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Warren RM. Recent findings in antibiotic resistance in TB. Joint IPCAN/IFIC. Invited talk presented at Spier, Stellenbosch, South Africa, 29 August - 1 September 2010.
  • Warren RM. Molecular Epidemiology and the Genesis of Drug-Resistant TB. Talk presented at the Workshop on Drug-Resistant TB: Current Practise, Controversies, and Clinical Challenges. University of Cape Town, Cape Town, South Africa, 3-5 September 2010.
  • Diacon AH. Emerging drugs for the management of drug-resistant TB. Talk presented at the Workshop on Drug-Resistant TB: Current Practise, Controversies, and Clinical Challenges. University of Cape Town, Cape Town, South Africa, 3-5 September 2010.
  • Ronacher K, Kleynhans L, Ehlers L, Walzl G. Medroxyprogesterone-acetate alters cytokine expression in response to mycobacterium bovis BCG in vitro and in PBMCs of contraceptive users. Talk presented at the SATBAT Meeting, Johannesburg, South Africa, 12 October 2010.
  • Ronacher K, Kleynhans L, Ehlers L, Walzl G. Medroxyprogesterone-acetate alters cytokine expression in response to mycobacterium bovis BCG in vitro and in PBMCs of contraceptive users. Talk presented at the South African Immunology Society Conference. Lanseria, South Africa, 5-8 Dec 2010.
  • Walzl G, Chegou NN, Loxton A, Ronacher K. Biomarkers for different forms of tuberculosis. Talk presented at the South African Immunology Society Conference. Lanseria, South Africa, 5-8 December 2010.
  • De Souza GA, Fortuin S, Aguilar D, Hernandez-Pando R, McEvoy CRE, Van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG. Using a label-free proteomic method to identify differentially abundant proteins in closely related hypo and hyper-virulent clinical Mycobacterium tuberculosis Beijing isolates. Talk presented at the Analitika International Conference on Analytical Science, Stellenbosch, South Africa, 5 - 9 December 2010.
  • Gey van Pittius NC. Tuberculosis in South Africa- problem setting - epidemiology data and current trends. Invited talk presented at Koch-Metschnikow Forum Fourth Scientific Symposium on the occasion of World Tuberculosis Day 2010. Berlin, Germany, March 22-23 2010.
  • Warren RM, Gey van Pittius NC, Streicher EM, Louw GE, Calver A, Hernando-Pandez R, De Vos M, Tait M, Trollip A, Chiota V, Hanekom M, Coetzee G, Van der Spuy G, Muller B, Victor TC, Van Helden PD. Mycobacterium tuberculosis adaptation: answers or questions. Invited talk presented at the TB Adapt meeting. Rio De Janeiro, Brazil, 25-26 May 2010.
  • Warren RM, Gey van Pittius NC, Streicher EM, Louw GE, Calver A, Hernando-Pandez R, De Vos M, Tait M, Trollip A, Chiota V, Hanekom M, Coetzee G, Van der Spuy G, Muller B, Victor TC van Helden PD. A compilation of recent findings in molecular epidemiology and diagnostics of tuberculosis in South Africa. Talk presented at the IV National Tuberculosis Meeting/ 1st Brazilian STOP TB Partners’ Forum. Rio De Janeiro, Brazil, 26 - 29 May 2010.
  • Gey van Pittius NC. Tuberculosis in South Africa - from a treatable disease to total drug resistance. Invited talk presented at the Institute of Medicine (IOM) of the USA and the Russian Academy of Medical Sciences (RAMS) Forum on Drug Discovery, Development, and Translation. Moscow, Russia, 26-27 May 2010.
  • Victor TC, Streicher EM, Tait M, Johnson R, Van der Spuy GD, Gey van Pittius NC, Theron D, Bosman M, Coetzee GJ, Van Helden PD, Chabula-Nxiweni EM, Trollip A, Warren RM. Molecular insights Into the XDR-TB Epidemic in the Western And Eastern Cape Regions of South Africa. Invited talk presented at the ASM conference. San Diego, USA, 23-27 May 2010.
  • Louw GE, Warren RM, Gey van Pittius NC, McEvoy CRE, Murray M, Van Helden PD, Victor TC. Activated efflux pumps define the level of Rifampicin resistance in M. tuberculosis. Invited talk presented at the ASM Conference, San Diego, USA, 23-27 May 2010.
  • Victor TC. Molecular insights Into the XDR-TB Epidemic in the Western and Eastern Cape Regions of South Africa. Invited speaker at the Global Forum for infectious diseases. University California, San Diego, USA, 23-27 May 2010.
  • Van Helden. Susceptibility testing of Mycobacterium tuberculosis: The why and how in the developing world. Invited talk presented at the 50th Interscience conference on antimicrobial agents and chemotherapy. Boston, USA, 12-15 September 2010.
  • Victor TC. Insights into MDR- and XDR-TB in South Africa. Invited talk presented at a meeting to discuss the application of genetics- and genomics-based tools for diagnosis, drug susceptibility testing and epidemiology. Tunis, Tunisia, 20-22 September 2010.
  • Diacon AH. TMC-207 versus placebo plus OBT for the treatment of MDR-TB: a prospective clinical trial. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Van Helden PD. Microbial genetic determinants of TB infection and disease progression. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Walzl G. Biomarkers for use in clinical trials. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • Stead M. Frequency and clinical outcome of mixed infections in South African TB patients. Invited talk presented at the 41st Union World Conference on Lung Health. Berlin, Germany, 11-15 November 2010.
  • De Vos M, Warren RM, Johnson R, Van Helden PD, Ndimba BK, Victor TC. RIF induces diferential protein expression in Mycobacterium tuberculosis. Poster presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Ngwane A, Wiid IJF, Van Helden PD. Elucidation of the mechanism of action of a novel furanone based antituberculosis compound (F1082). Poster presented at the 10th AstraZeneca Health Sciences Research Day. University of Stellenbosch, South Africa, 25 February 2010.
  • Fortuin S. A label-free method identified differentially abundant proteins in related M. tuberculosis Beijing strains, Poster presented at the ESF 4th conference on Functional Genomics and Disease, Dresden, Germany, 14–17 April 2010.
  • Bruiners N, Gey Van Pittius NC, Warren RM. Investigation Of Complement C1q Polymorphisms In A Cohort Of Tuberculosis Infected Patients. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Dramowski A, Morsheimer MM, Jordaan A, Victor TC, Donald PR, Schaaf HS. Rifampicin Mono-Resistant Mycobacterium Tuberculosis Disease: An Increasing Trend Among Children In Cape Town. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Fang Z, Gey Van Pittius NC, Van Helden PD. Elucidation Of The Substrates Of Mycosin 3, An Essential Protease of M.Tuberculosis. Poster presented at the 54th Academic Year Day, University Of Stellenbosch, South Africa, 11-12 August 2010.
  • Friedrich SO, Nabeta P, Boehme C, Venter A, Diacon AH.  Direct comparison of GeneXpert with smear microscopy, solid and liquid culture for sputum monitoring of tuberculosis (TB) patients. Poster presented at the  54th Annual Academic Day, Stellenbosch University, South Africa, 11-12 August 2010.
  • Grobbelaar M, Louw GE, Warren RM, Gey Van Pittius NC, Van Helden PD, Victor TC. New hope for treatment of drug resistant Tuberculosis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Kirsten CJ, Hayward D, Wiid IJF, Van Helden PD. Identification of the Transcriptional Regulator of Nitrogen Related Genes In Mycobacterium Smegmatis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Lucas LA, Möller M, Nebel A, Van Helden PD, Schreiber S, Hoal EG. Genetic Variants of Toll- Like Receptors 2 & 8 And Their Possible Role In Susceptibility To Pulmonary Tuberculosis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Newton-Foot M, Warren RM, Van Helden PD, Gey Van Pittius NC. The Mycobacterium Tuberculosis Esx-3 Secretion System Interactome. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Phalane KG, Esterhuyse MM, Hoal EG. Evaluation of Differential Dna Methylation Levels In The Interleukin 8 (Il8) Gene Of Tuberculosis Infected Cases And Controls. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Seepe PM, Warren RM, Johnson R, Van Helden PD, Louw GE, Victor TC. Differential Expression Of Genes In Clinical Strains of Mycobacterium Tuberculosis In Response to Isoniazid. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Siame KK, Gey Van Pittius NC, Warren RM. Investigating the Genome Variation and Evolution of Group 1 M. Tuberculosis Strains. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Steyn NL, Smit M, Newton-Foot M, Gey Van Pittius NC. The Construction of Genetic Knock-Outs of the Esx Secretion Systems In Mycobacterium Smegmatis. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Viljoen AJ, Kirsten C, Hayward D, Wiid IJF, Van Helden PD. Regulation of the Nitrogen Assimilatory Enzymes, Glutamine Synthetase And Glutamate Dehydrogenase In Mycobacterium Smegmatis And M. Bovis BCG. Poster presented at the 54th Academic Year Day, University of Stellenbosch, South Africa, 11-12 August 2010.
  • Seepe PM, Johnson R, Louw GE, Van Helden PD, Warren RM, Victor TC. Differential expression of genes in clinical strains of Mycobacterium Tuberculosis in response to isoniazid. Poster presented at the MRC Research Day, MRC Conference Centre, Cape Town, South Africa, 14-15 October 2010.
  • De Souza GA, Fortuin S, Aguilar D, Hernandez-Pando R, McEvoy CRE, Van Helden PD, Koehler CJ, Thiede B, Warren RM, Wiker HG. A label-free method identified differentially abundant proteins in related M. tuberculosis Beijing strains. Poster presented at the ESF 4th conference on Functional Genomics and Disease, Dresden, Germany, 14 – 17 April 2010.
  • Möller M, Flachsbart F, Till A, Thye T, Horstmann R, Meyer C, Osei I, Van Helden PD, Hoal EG, Schreiber S, Nebel A, Franke A. A functional haplotype in the 3’UTR of TNFRSF1B is associated with TB in two African populations. Poster presented at the EMBO|EMBL Symposium Human Variation: Cause and Consequence, Heidelberg, Germany, 20-23 June 2010.
  • Hoal EG, Cobat A, Gallant C, Simkin L, Black GF, Stanley K, Hughes J, Doherty T, Hanekom W, Eley B, Beyers N, Jaïs J, Boland-Auge A, Van Helden PD, Casanova J, Abel L, Alcaïs A, Schurr E. A Genome-Wide Linkage Search for Genetic Loci Controlling Resistance to Tuberculosis (1001/W). Poster presented at the 60th Annual Meeting of The American Society of Human Genetics, Washington DC, USA, 2-6 November 2010.
  • Möller M, De Wit E, Delport W, Harmant C, Rugamika CE, Quach H, Meintjes A, Balanovsky O, Zaporozhchenko V, Bormans C, Van Helden PD, Seioghe C, Behar DM, Hoal EG. The History in our genes: The complex structure of the South African Coloured population. Poster presented at the ASHG 60th Annual Meeting in Washington DC, USA, 2-6 November 2010.
  • Salie M, Möller M, Van Helden PD, Schreiber S, Hoal EG, Nebel A, Hofmann S. A 3’UTR polymorphism in ANXA11 is associated with pulmonary tuberculosis in the South African coloured population. Poster presented at the ASHG 60th Annual Meeting in Washington DC, USA, 2-6 November 2010. 

Other Relevant Outputs (including honours and awards to staff)

  1. Prof. Mizrahi delivered the Division U Mycobacteriology Lecture at the 110th Meeting of the American Society for Microbiology in San Diego, on 24th May 2010. This honour is given to a scientist who has made a significant contribution to the field of mycobacteriology, and is accompanied by the award of a certificate to mark the occasion.
  2. Prof. Mizrahi was appointed to three-year terms on the Board of Directors and the Scientific Advisory Board of K-RITH, UKZN.
  3. Dr. Bavesh Kana was appointed as the new Head of the Wits University node of the CBTBR, effective 1 January 2011.
  4. Eileen Hoal-van Helden received the SU Rector’s award for Excellence in Research in November 2010
  5. Warren, RM. Obtained a B2 rating by the NRF (2010)
  6. Gey van Pittius, NC was elected as a member of the Academy of Science of South Africa (ASSAf)
  7. Specialist diagnostic service for suspect extra-pulmonary TB cases
  8. Hospital medical specialist clinical services, eg pulmonology and genetics
  9. Advice to National Dept Agriculture on GMOs and veterinary TB
  10. Seegene (Korea): We have been approached and are currently in a collaborative venture with Seegene, Inc. to design and improve M. tuberculosis diagnostics.  Seegene is a molecular diagnostic company based in Korea well known for its pioneering R & D activities and novel technologies. Seegene, Inc. has continued to develop innovative technology platforms, ACP™ (Annealing Control Primer), DPO™ (Dual Priming Oligonucleotide), and READ™ (Real Amplicon Detection). With these cutting-edge molecular diagnostic technologies applied to diagnostic kits and various practical approaches, Seegene has enhanced the sensitivity and specificity of PCR (polymerase chain reaction) up to unprecedented level. This advance enables Seegene Inc. to provide multiplex PCR products by which multiple target genes of pathogens can be simultaneously detected, saving time and cost.
  11. AID (Germany): Evaluation of a genetic drug susceptibility test.
  12. Hain Life Sciences (Germany): Evaluation of the MDRTBsl genetic drug susceptibility test.

Research


© CBTBR 2006 | Last updated: 17 August, 2015